Amino acid dipepetide frequency for Mycolicibacterium insubricum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.756AlaAla: 21.756 ± 0.218	0.912AlaCys: 0.912 ± 0.027	9.102AlaAsp: 9.102 ± 0.106	8.183AlaGlu: 8.183 ± 0.1	3.324AlaPhe: 3.324 ± 0.056	13.049AlaGly: 13.049 ± 0.123	2.542AlaHis: 2.542 ± 0.046	5.109AlaIle: 5.109 ± 0.063	2.926AlaLys: 2.926 ± 0.065	13.334AlaLeu: 13.334 ± 0.13	2.898AlaMet: 2.898 ± 0.052	2.698AlaAsn: 2.698 ± 0.051	7.124AlaPro: 7.124 ± 0.099	3.843AlaGln: 3.843 ± 0.052	8.808AlaArg: 8.808 ± 0.088	5.502AlaSer: 5.502 ± 0.065	7.816AlaThr: 7.816 ± 0.084	12.178AlaVal: 12.178 ± 0.116	1.597AlaTrp: 1.597 ± 0.035	2.295AlaTyr: 2.295 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.093CysAla: 1.093 ± 0.034	0.084CysCys: 0.084 ± 0.006	0.557CysAsp: 0.557 ± 0.018	0.376CysGlu: 0.376 ± 0.016	0.233CysPhe: 0.233 ± 0.012	0.923CysGly: 0.923 ± 0.028	0.192CysHis: 0.192 ± 0.012	0.244CysIle: 0.244 ± 0.014	0.133CysLys: 0.133 ± 0.01	0.636CysLeu: 0.636 ± 0.024	0.14CysMet: 0.14 ± 0.01	0.193CysAsn: 0.193 ± 0.013	0.512CysPro: 0.512 ± 0.022	0.215CysGln: 0.215 ± 0.013	0.554CysArg: 0.554 ± 0.021	0.47CysSer: 0.47 ± 0.019	0.5CysThr: 0.5 ± 0.022	0.582CysVal: 0.582 ± 0.02	0.123CysTrp: 0.123 ± 0.01	0.2CysTyr: 0.2 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	8.307AspAla: 8.307 ± 0.092	0.489AspCys: 0.489 ± 0.02	4.472AspAsp: 4.472 ± 0.065	3.874AspGlu: 3.874 ± 0.064	1.812AspPhe: 1.812 ± 0.039	6.564AspGly: 6.564 ± 0.082	1.293AspHis: 1.293 ± 0.03	2.635AspIle: 2.635 ± 0.049	1.251AspLys: 1.251 ± 0.034	6.051AspLeu: 6.051 ± 0.076	0.969AspMet: 0.969 ± 0.027	1.364AspAsn: 1.364 ± 0.034	4.836AspPro: 4.836 ± 0.062	1.677AspGln: 1.677 ± 0.035	4.594AspArg: 4.594 ± 0.062	2.664AspSer: 2.664 ± 0.044	3.456AspThr: 3.456 ± 0.06	4.967AspVal: 4.967 ± 0.07	1.052AspTrp: 1.052 ± 0.03	1.53AspTyr: 1.53 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	5.992GluAla: 5.992 ± 0.088	0.361GluCys: 0.361 ± 0.016	2.422GluAsp: 2.422 ± 0.046	2.339GluGlu: 2.339 ± 0.044	1.896GluPhe: 1.896 ± 0.041	2.964GluGly: 2.964 ± 0.051	1.5GluHis: 1.5 ± 0.032	2.607GluIle: 2.607 ± 0.048	1.209GluLys: 1.209 ± 0.034	6.671GluLeu: 6.671 ± 0.077	1.027GluMet: 1.027 ± 0.028	1.117GluAsn: 1.117 ± 0.031	3.086GluPro: 3.086 ± 0.058	2.086GluGln: 2.086 ± 0.043	3.945GluArg: 3.945 ± 0.062	2.6GluSer: 2.6 ± 0.046	2.67GluThr: 2.67 ± 0.054	4.204GluVal: 4.204 ± 0.058	0.632GluTrp: 0.632 ± 0.022	1.091GluTyr: 1.091 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	3.971PheAla: 3.971 ± 0.063	0.309PheCys: 0.309 ± 0.016	2.478PheAsp: 2.478 ± 0.047	1.415PheGlu: 1.415 ± 0.038	0.941PhePhe: 0.941 ± 0.028	3.464PheGly: 3.464 ± 0.056	0.61PheHis: 0.61 ± 0.02	1.086PheIle: 1.086 ± 0.03	0.515PheLys: 0.515 ± 0.019	2.484PheLeu: 2.484 ± 0.053	0.455PheMet: 0.455 ± 0.018	0.795PheAsn: 0.795 ± 0.022	1.402PhePro: 1.402 ± 0.036	0.666PheGln: 0.666 ± 0.025	1.636PheArg: 1.636 ± 0.037	1.574PheSer: 1.574 ± 0.035	2.179PheThr: 2.179 ± 0.044	2.426PheVal: 2.426 ± 0.047	0.441PheTrp: 0.441 ± 0.018	0.689PheTyr: 0.689 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	10.815GlyAla: 10.815 ± 0.102	0.836GlyCys: 0.836 ± 0.03	5.22GlyAsp: 5.22 ± 0.073	4.365GlyGlu: 4.365 ± 0.059	3.055GlyPhe: 3.055 ± 0.051	8.1GlyGly: 8.1 ± 0.119	2.088GlyHis: 2.088 ± 0.036	4.341GlyIle: 4.341 ± 0.056	2.245GlyLys: 2.245 ± 0.047	8.905GlyLeu: 8.905 ± 0.105	2.213GlyMet: 2.213 ± 0.046	2.128GlyAsn: 2.128 ± 0.054	4.991GlyPro: 4.991 ± 0.065	2.785GlyGln: 2.785 ± 0.048	6.46GlyArg: 6.46 ± 0.072	5.332GlySer: 5.332 ± 0.067	5.788GlyThr: 5.788 ± 0.074	7.624GlyVal: 7.624 ± 0.075	1.715GlyTrp: 1.715 ± 0.037	2.596GlyTyr: 2.596 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	2.335HisAla: 2.335 ± 0.035	0.222HisCys: 0.222 ± 0.013	1.314HisAsp: 1.314 ± 0.035	0.933HisGlu: 0.933 ± 0.029	0.657HisPhe: 0.657 ± 0.021	2.043HisGly: 2.043 ± 0.039	0.637HisHis: 0.637 ± 0.025	0.812HisIle: 0.812 ± 0.021	0.337HisLys: 0.337 ± 0.016	2.032HisLeu: 2.032 ± 0.039	0.318HisMet: 0.318 ± 0.015	0.491HisAsn: 0.491 ± 0.015	1.684HisPro: 1.684 ± 0.034	0.628HisGln: 0.628 ± 0.022	2.011HisArg: 2.011 ± 0.045	1.017HisSer: 1.017 ± 0.03	1.251HisThr: 1.251 ± 0.033	1.425HisVal: 1.425 ± 0.033	0.369HisTrp: 0.369 ± 0.019	0.554HisTyr: 0.554 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.465IleAla: 6.465 ± 0.077	0.364IleCys: 0.364 ± 0.015	3.482IleAsp: 3.482 ± 0.058	2.46IleGlu: 2.46 ± 0.043	1.023IlePhe: 1.023 ± 0.031	4.575IleGly: 4.575 ± 0.063	0.721IleHis: 0.721 ± 0.02	1.545IleIle: 1.545 ± 0.039	0.905IleLys: 0.905 ± 0.028	3.078IleLeu: 3.078 ± 0.054	0.604IleMet: 0.604 ± 0.022	1.146IleAsn: 1.146 ± 0.033	2.454IlePro: 2.454 ± 0.042	0.871IleGln: 0.871 ± 0.025	2.702IleArg: 2.702 ± 0.042	2.224IleSer: 2.224 ± 0.041	3.015IleThr: 3.015 ± 0.048	3.529IleVal: 3.529 ± 0.049	0.477IleTrp: 0.477 ± 0.018	0.836IleTyr: 0.836 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	2.541LysAla: 2.541 ± 0.06	0.124LysCys: 0.124 ± 0.01	1.059LysAsp: 1.059 ± 0.03	0.877LysGlu: 0.877 ± 0.028	0.614LysPhe: 0.614 ± 0.024	1.448LysGly: 1.448 ± 0.038	0.437LysHis: 0.437 ± 0.019	0.991LysIle: 0.991 ± 0.03	0.639LysLys: 0.639 ± 0.031	2.141LysLeu: 2.141 ± 0.048	0.523LysMet: 0.523 ± 0.021	0.541LysAsn: 0.541 ± 0.02	1.434LysPro: 1.434 ± 0.034	0.661LysGln: 0.661 ± 0.025	1.495LysArg: 1.495 ± 0.04	1.11LysSer: 1.11 ± 0.032	1.386LysThr: 1.386 ± 0.041	1.828LysVal: 1.828 ± 0.042	0.287LysTrp: 0.287 ± 0.016	0.463LysTyr: 0.463 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	14.79LeuAla: 14.79 ± 0.13	0.827LeuCys: 0.827 ± 0.027	6.503LeuAsp: 6.503 ± 0.069	4.033LeuGlu: 4.033 ± 0.056	2.597LeuPhe: 2.597 ± 0.049	9.026LeuGly: 9.026 ± 0.085	2.028LeuHis: 2.028 ± 0.041	4.24LeuIle: 4.24 ± 0.059	1.669LeuLys: 1.669 ± 0.038	9.967LeuLeu: 9.967 ± 0.126	1.806LeuMet: 1.806 ± 0.036	2.186LeuAsn: 2.186 ± 0.048	5.862LeuPro: 5.862 ± 0.072	2.295LeuGln: 2.295 ± 0.047	7.424LeuArg: 7.424 ± 0.076	5.562LeuSer: 5.562 ± 0.059	6.705LeuThr: 6.705 ± 0.087	8.366LeuVal: 8.366 ± 0.099	1.225LeuTrp: 1.225 ± 0.034	1.639LeuTyr: 1.639 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	2.687MetAla: 2.687 ± 0.046	0.186MetCys: 0.186 ± 0.013	0.887MetAsp: 0.887 ± 0.027	0.725MetGlu: 0.725 ± 0.024	0.661MetPhe: 0.661 ± 0.026	1.479MetGly: 1.479 ± 0.036	0.398MetHis: 0.398 ± 0.018	0.954MetIle: 0.954 ± 0.026	0.437MetLys: 0.437 ± 0.018	2.039MetLeu: 2.039 ± 0.04	0.445MetMet: 0.445 ± 0.018	0.559MetAsn: 0.559 ± 0.022	1.226MetPro: 1.226 ± 0.029	0.524MetGln: 0.524 ± 0.021	1.446MetArg: 1.446 ± 0.03	1.511MetSer: 1.511 ± 0.03	1.856MetThr: 1.856 ± 0.037	1.607MetVal: 1.607 ± 0.04	0.249MetTrp: 0.249 ± 0.014	0.341MetTyr: 0.341 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.741AsnAla: 2.741 ± 0.052	0.213AsnCys: 0.213 ± 0.014	1.291AsnAsp: 1.291 ± 0.03	0.963AsnGlu: 0.963 ± 0.027	0.705AsnPhe: 0.705 ± 0.025	2.123AsnGly: 2.123 ± 0.055	0.484AsnHis: 0.484 ± 0.019	0.985AsnIle: 0.985 ± 0.03	0.508AsnLys: 0.508 ± 0.019	2.175AsnLeu: 2.175 ± 0.045	0.452AsnMet: 0.452 ± 0.018	0.621AsnAsn: 0.621 ± 0.026	1.933AsnPro: 1.933 ± 0.036	0.684AsnGln: 0.684 ± 0.023	1.637AsnArg: 1.637 ± 0.034	1.206AsnSer: 1.206 ± 0.033	1.46AsnThr: 1.46 ± 0.041	1.756AsnVal: 1.756 ± 0.039	0.402AsnTrp: 0.402 ± 0.017	0.602AsnTyr: 0.602 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	8.23ProAla: 8.23 ± 0.106	0.304ProCys: 0.304 ± 0.014	4.677ProAsp: 4.677 ± 0.066	3.84ProGlu: 3.84 ± 0.061	1.585ProPhe: 1.585 ± 0.035	6.411ProGly: 6.411 ± 0.086	1.118ProHis: 1.118 ± 0.029	2.156ProIle: 2.156 ± 0.045	1.258ProLys: 1.258 ± 0.032	4.887ProLeu: 4.887 ± 0.067	1.277ProMet: 1.277 ± 0.031	1.353ProAsn: 1.353 ± 0.039	3.423ProPro: 3.423 ± 0.08	1.826ProGln: 1.826 ± 0.042	3.472ProArg: 3.472 ± 0.047	3.123ProSer: 3.123 ± 0.064	3.457ProThr: 3.457 ± 0.057	5.429ProVal: 5.429 ± 0.069	0.852ProTrp: 0.852 ± 0.026	1.155ProTyr: 1.155 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	3.314GlnAla: 3.314 ± 0.063	0.206GlnCys: 0.206 ± 0.014	1.148GlnAsp: 1.148 ± 0.029	1.016GlnGlu: 1.016 ± 0.026	0.904GlnPhe: 0.904 ± 0.025	1.852GlnGly: 1.852 ± 0.041	0.694GlnHis: 0.694 ± 0.025	1.502GlnIle: 1.502 ± 0.034	0.582GlnLys: 0.582 ± 0.022	3.363GlnLeu: 3.363 ± 0.055	0.643GlnMet: 0.643 ± 0.024	0.636GlnAsn: 0.636 ± 0.025	1.788GlnPro: 1.788 ± 0.04	1.214GlnGln: 1.214 ± 0.035	2.658GlnArg: 2.658 ± 0.045	1.428GlnSer: 1.428 ± 0.031	1.586GlnThr: 1.586 ± 0.035	2.471GlnVal: 2.471 ± 0.047	0.514GlnTrp: 0.514 ± 0.018	0.63GlnTyr: 0.63 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	8.428ArgAla: 8.428 ± 0.093	0.575ArgCys: 0.575 ± 0.024	4.239ArgAsp: 4.239 ± 0.056	3.674ArgGlu: 3.674 ± 0.052	2.361ArgPhe: 2.361 ± 0.044	5.278ArgGly: 5.278 ± 0.073	1.691ArgHis: 1.691 ± 0.038	3.592ArgIle: 3.592 ± 0.048	1.437ArgLys: 1.437 ± 0.036	7.331ArgLeu: 7.331 ± 0.084	1.79ArgMet: 1.79 ± 0.036	1.698ArgAsn: 1.698 ± 0.037	4.035ArgPro: 4.035 ± 0.06	1.993ArgGln: 1.993 ± 0.045	6.625ArgArg: 6.625 ± 0.094	3.82ArgSer: 3.82 ± 0.055	4.248ArgThr: 4.248 ± 0.06	5.483ArgVal: 5.483 ± 0.064	1.3ArgTrp: 1.3 ± 0.036	1.899ArgTyr: 1.899 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	6.968SerAla: 6.968 ± 0.076	0.421SerCys: 0.421 ± 0.019	3.056SerAsp: 3.056 ± 0.051	2.304SerGlu: 2.304 ± 0.045	1.591SerPhe: 1.591 ± 0.034	5.677SerGly: 5.677 ± 0.077	0.977SerHis: 0.977 ± 0.025	2.016SerIle: 2.016 ± 0.045	1.055SerLys: 1.055 ± 0.035	4.461SerLeu: 4.461 ± 0.058	1.316SerMet: 1.316 ± 0.036	1.13SerAsn: 1.13 ± 0.029	3.089SerPro: 3.089 ± 0.06	1.38SerGln: 1.38 ± 0.033	3.398SerArg: 3.398 ± 0.047	2.924SerSer: 2.924 ± 0.048	3.327SerThr: 3.327 ± 0.049	4.436SerVal: 4.436 ± 0.057	0.949SerTrp: 0.949 ± 0.028	1.268SerTyr: 1.268 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	8.714ThrAla: 8.714 ± 0.095	0.44ThrCys: 0.44 ± 0.016	4.033ThrAsp: 4.033 ± 0.061	3.366ThrGlu: 3.366 ± 0.062	1.782ThrPhe: 1.782 ± 0.035	6.676ThrGly: 6.676 ± 0.085	1.152ThrHis: 1.152 ± 0.029	2.313ThrIle: 2.313 ± 0.041	1.219ThrLys: 1.219 ± 0.035	5.843ThrLeu: 5.843 ± 0.065	1.191ThrMet: 1.191 ± 0.032	1.239ThrAsn: 1.239 ± 0.033	4.088ThrPro: 4.088 ± 0.059	1.425ThrGln: 1.425 ± 0.034	3.785ThrArg: 3.785 ± 0.05	2.984ThrSer: 2.984 ± 0.054	3.95ThrThr: 3.95 ± 0.07	6.34ThrVal: 6.34 ± 0.068	0.813ThrTrp: 0.813 ± 0.027	1.214ThrTyr: 1.214 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	11.822ValAla: 11.822 ± 0.1	0.692ValCys: 0.692 ± 0.021	5.715ValAsp: 5.715 ± 0.068	4.17ValGlu: 4.17 ± 0.06	2.55ValPhe: 2.55 ± 0.044	6.919ValGly: 6.919 ± 0.08	1.683ValHis: 1.683 ± 0.04	4.012ValIle: 4.012 ± 0.057	1.657ValLys: 1.657 ± 0.041	9.096ValLeu: 9.096 ± 0.096	1.549ValMet: 1.549 ± 0.038	2.093ValAsn: 2.093 ± 0.044	4.702ValPro: 4.702 ± 0.061	2.036ValGln: 2.036 ± 0.041	5.643ValArg: 5.643 ± 0.069	4.596ValSer: 4.596 ± 0.063	5.782ValThr: 5.782 ± 0.065	8.209ValVal: 8.209 ± 0.094	1.029ValTrp: 1.029 ± 0.028	1.621ValTyr: 1.621 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.591TrpAla: 1.591 ± 0.036	0.179TrpCys: 0.179 ± 0.012	0.8TrpAsp: 0.8 ± 0.023	0.586TrpGlu: 0.586 ± 0.022	0.49TrpPhe: 0.49 ± 0.022	0.986TrpGly: 0.986 ± 0.027	0.364TrpHis: 0.364 ± 0.017	0.641TrpIle: 0.641 ± 0.023	0.273TrpLys: 0.273 ± 0.014	1.765TrpLeu: 1.765 ± 0.041	0.314TrpMet: 0.314 ± 0.015	0.41TrpAsn: 0.41 ± 0.018	0.82TrpPro: 0.82 ± 0.025	0.623TrpGln: 0.623 ± 0.02	1.21TrpArg: 1.21 ± 0.027	0.924TrpSer: 0.924 ± 0.026	0.9TrpThr: 0.9 ± 0.029	1.174TrpVal: 1.174 ± 0.033	0.346TrpTrp: 0.346 ± 0.018	0.33TrpTyr: 0.33 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.433TyrAla: 2.433 ± 0.044	0.218TyrCys: 0.218 ± 0.013	1.402TyrAsp: 1.402 ± 0.035	0.922TyrGlu: 0.922 ± 0.026	0.757TyrPhe: 0.757 ± 0.023	2.047TyrGly: 2.047 ± 0.047	0.462TyrHis: 0.462 ± 0.02	0.695TyrIle: 0.695 ± 0.023	0.344TyrLys: 0.344 ± 0.017	2.492TyrLeu: 2.492 ± 0.047	0.286TyrMet: 0.286 ± 0.015	0.541TyrAsn: 0.541 ± 0.02	1.343TyrPro: 1.343 ± 0.034	0.718TyrGln: 0.718 ± 0.022	1.99TyrArg: 1.99 ± 0.043	1.171TyrSer: 1.171 ± 0.029	1.237TyrThr: 1.237 ± 0.028	1.541TyrVal: 1.541 ± 0.036	0.356TyrTrp: 0.356 ± 0.018	0.535TyrTyr: 0.535 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4200 proteins (1350420 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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