Hasllibacter halocynthiae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Hasllibacter

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2723 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0X870|A0A2T0X870_9RHOB Phospho-2-dehydro-3-deoxyheptonate aldolase OS=Hasllibacter halocynthiae OX=595589 GN=BCF33_0740 PE=3 SV=1
MM1 pKa = 7.58KK2 pKa = 10.24KK3 pKa = 10.2LATATVLSASVAMPALAGGLDD24 pKa = 3.56QPVIAPVIVPPAPIAYY40 pKa = 9.19APQWTGGFIGASVGYY55 pKa = 9.71IDD57 pKa = 5.34SDD59 pKa = 4.02GNLVSVVTDD68 pKa = 3.52ADD70 pKa = 3.79GGTTEE75 pKa = 4.63VNDD78 pKa = 3.49VDD80 pKa = 5.27LSGDD84 pKa = 3.29GLTYY88 pKa = 10.78GLRR91 pKa = 11.84AGYY94 pKa = 10.1DD95 pKa = 3.34YY96 pKa = 11.14QFRR99 pKa = 11.84NGAVLGGVLQYY110 pKa = 11.47DD111 pKa = 4.32RR112 pKa = 11.84LDD114 pKa = 3.87LDD116 pKa = 3.73IGISDD121 pKa = 3.93EE122 pKa = 4.16QVDD125 pKa = 3.54IDD127 pKa = 3.82EE128 pKa = 4.92SANSVLRR135 pKa = 11.84AGLRR139 pKa = 11.84GGYY142 pKa = 9.34GAGANLFYY150 pKa = 10.6LTGGWARR157 pKa = 11.84LDD159 pKa = 3.6TDD161 pKa = 3.61VSGDD165 pKa = 3.2GDD167 pKa = 4.25GYY169 pKa = 10.91FAGLGYY175 pKa = 10.58EE176 pKa = 4.5RR177 pKa = 11.84LVTDD181 pKa = 3.62NVSVGIEE188 pKa = 3.93ALYY191 pKa = 11.0HH192 pKa = 6.03EE193 pKa = 5.69FDD195 pKa = 4.75DD196 pKa = 5.23FDD198 pKa = 4.17NADD201 pKa = 3.71VEE203 pKa = 4.52VDD205 pKa = 3.21ATTVGLNVNYY215 pKa = 10.45RR216 pKa = 11.84FF217 pKa = 3.79

Molecular weight: 22.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0X3N9|A0A2T0X3N9_9RHOB Beta-barrel assembly complex subunit BamF OS=Hasllibacter halocynthiae OX=595589 GN=BCF33_2435 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.21RR41 pKa = 11.84LSAA44 pKa = 3.93

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2723

0

2723

841267

30

3421

308.9

33.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.748 ± 0.072

0.774 ± 0.012

5.989 ± 0.042

6.475 ± 0.043

3.307 ± 0.03

10.087 ± 0.045

1.891 ± 0.021

3.981 ± 0.035

1.934 ± 0.035

10.155 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.454 ± 0.024

1.777 ± 0.022

5.908 ± 0.044

2.276 ± 0.022

8.414 ± 0.062

4.061 ± 0.029

4.825 ± 0.041

7.682 ± 0.039

1.476 ± 0.019

1.788 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski