Rhizobium straminoryzae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5046 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A549TD17|A0A549TD17_9RHIZ Uncharacterized protein OS=Rhizobium straminoryzae OX=1387186 GN=FNA46_07900 PE=4 SV=1
MM1 pKa = 7.74EE2 pKa = 4.63EE3 pKa = 4.74FGWFCPGIGYY13 pKa = 7.26WQSISWPDD21 pKa = 3.38DD22 pKa = 3.3EE23 pKa = 5.32TRR25 pKa = 11.84AAYY28 pKa = 9.09PPGTVQVPLKK38 pKa = 10.39PMPTTEE44 pKa = 4.29YY45 pKa = 10.59IDD47 pKa = 3.76WTWSGSEE54 pKa = 4.07WIGVPRR60 pKa = 11.84PAEE63 pKa = 3.9PAPP66 pKa = 3.75

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A549TH24|A0A549TH24_9RHIZ ABC transporter permease subunit OS=Rhizobium straminoryzae OX=1387186 GN=FNA46_02725 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.22GGRR28 pKa = 11.84KK29 pKa = 9.06VLSARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5046

0

5046

1624162

28

2824

321.9

34.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.328 ± 0.045

0.774 ± 0.012

5.588 ± 0.031

5.587 ± 0.034

3.829 ± 0.025

8.423 ± 0.032

2.046 ± 0.018

5.422 ± 0.025

3.276 ± 0.027

10.404 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.573 ± 0.018

2.623 ± 0.018

5.023 ± 0.025

3.21 ± 0.021

7.046 ± 0.035

5.615 ± 0.028

5.388 ± 0.021

7.356 ± 0.027

1.263 ± 0.014

2.224 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski