Martelella sp. BGMRC2036
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4394 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A506U505|A0A506U505_9RHIZ Cytochrome bo(3) ubiquinol oxidase subunit 4 OS=Martelella sp. BGMRC2036 OX=2590451 GN=cyoD PE=3 SV=1
MM1 pKa = 7.11 LQRR4 pKa = 11.84 RR5 pKa = 11.84 EE6 pKa = 4.11 LRR8 pKa = 11.84 LLPPSLSISKK18 pKa = 10.72 SMTGPHH24 pKa = 6.62 LNLEE28 pKa = 4.25 YY29 pKa = 10.35 RR30 pKa = 11.84 RR31 pKa = 11.84 SYY33 pKa = 11.01 DD34 pKa = 4.17 YY35 pKa = 11.33 EE36 pKa = 4.24 DD37 pKa = 3.6 QPEE40 pKa = 4.43 DD41 pKa = 3.72 KK42 pKa = 10.98 SIDD45 pKa = 3.17 RR46 pKa = 11.84 GTRR49 pKa = 11.84 DD50 pKa = 3.2 WIAAEE55 pKa = 4.01 QPKK58 pKa = 10.11 VYY60 pKa = 10.53 HH61 pKa = 6.84 FIFTVGDD68 pKa = 3.54 DD69 pKa = 3.59 RR70 pKa = 11.84 LKK72 pKa = 10.34 VTGTDD77 pKa = 3.45 FDD79 pKa = 4.15 TGFLADD85 pKa = 4.02 MRR87 pKa = 11.84 SGNDD91 pKa = 4.97 FVTTWDD97 pKa = 3.72 NPYY100 pKa = 10.8 LPASTFWMGSGNDD113 pKa = 3.49 VANVCGSSPTSDD125 pKa = 3.2 EE126 pKa = 5.2 DD127 pKa = 3.45 EE128 pKa = 5.1 DD129 pKa = 4.62 EE130 pKa = 4.17 EE131 pKa = 4.58 VTRR134 pKa = 11.84 VLCDD138 pKa = 4.51 DD139 pKa = 4.95 GDD141 pKa = 5.0 DD142 pKa = 4.88 GDD144 pKa = 5.75 DD145 pKa = 4.37 GDD147 pKa = 5.94 DD148 pKa = 3.4 IVNITGGGYY157 pKa = 11.0 ANVFGDD163 pKa = 4.57 DD164 pKa = 4.56 GNDD167 pKa = 3.7 TLCVQQNGAFKK178 pKa = 10.78 SGVISLTGGAGYY190 pKa = 8.14 DD191 pKa = 3.5 TFLVTQHH198 pKa = 6.84 PSSSYY203 pKa = 11.07 NLPTSTGPASIGIFVTDD220 pKa = 3.12 VLQDD224 pKa = 3.47 VLGWVPGVGLATHH237 pKa = 7.18 AAAALISTTTMAARR251 pKa = 11.84 QSTRR255 pKa = 11.84 FLNEE259 pKa = 3.15 FSGAMDD265 pKa = 4.03 GQDD268 pKa = 2.83 GSVNIPQGGDD278 pKa = 3.17 TYY280 pKa = 11.78 VSIEE284 pKa = 4.47 DD285 pKa = 3.92 FDD287 pKa = 4.39 PLEE290 pKa = 4.52 DD291 pKa = 4.25 QLVQTYY297 pKa = 8.29 LHH299 pKa = 6.08 NTNGMEE305 pKa = 4.06 QYY307 pKa = 10.14 SWQSQDD313 pKa = 3.17 VASDD317 pKa = 3.38 NFSIAFKK324 pKa = 10.98 SDD326 pKa = 3.01 GTNVSSVIHH335 pKa = 6.12 VDD337 pKa = 3.28 PAIVEE342 pKa = 4.25 TLRR345 pKa = 11.84 GIEE348 pKa = 4.14 DD349 pKa = 3.19 AHH351 pKa = 6.96 GEE353 pKa = 4.18 RR354 pKa = 11.84 GSLPASDD361 pKa = 5.79 AEE363 pKa = 4.35 MKK365 pKa = 10.9 NKK367 pKa = 9.93 ILTSMVDD374 pKa = 3.37 SIVNIKK380 pKa = 10.62 KK381 pKa = 10.51 EE382 pKa = 3.99 GDD384 pKa = 3.48 DD385 pKa = 4.65 LYY387 pKa = 11.17 IMLQGNGGYY396 pKa = 10.31 DD397 pKa = 3.72 KK398 pKa = 11.33 QPVDD402 pKa = 3.49 TDD404 pKa = 3.67 QVEE407 pKa = 4.54 SALSAMQSSSSDD419 pKa = 3.46 LSSLIANMHH428 pKa = 6.46 NGDD431 pKa = 3.46 QYY433 pKa = 11.91 YY434 pKa = 11.1 LLGNTPVKK442 pKa = 10.64 LGGPSTDD449 pKa = 2.94 GATNITLGDD458 pKa = 3.49 NDD460 pKa = 4.93 SNVMFAGTTQDD471 pKa = 2.58 WVTDD475 pKa = 4.22 KK476 pKa = 11.35 NGHH479 pKa = 5.0 TSALEE484 pKa = 3.88 DD485 pKa = 3.66 HH486 pKa = 7.52 DD487 pKa = 4.02 GTFYY491 pKa = 10.7 PSQHH495 pKa = 5.43 MTSTMFGMGGDD506 pKa = 4.05 DD507 pKa = 3.79 IIYY510 pKa = 10.33 GSFGEE515 pKa = 4.31 DD516 pKa = 2.51 CVYY519 pKa = 11.1 AGDD522 pKa = 4.24 GNDD525 pKa = 4.16 MISTGAGNDD534 pKa = 3.98 YY535 pKa = 10.98 VWGDD539 pKa = 3.25 AGDD542 pKa = 4.91 DD543 pKa = 4.14 YY544 pKa = 11.6 ICGGRR549 pKa = 11.84 GDD551 pKa = 3.94 DD552 pKa = 3.93 TLFGGDD558 pKa = 3.7 GKK560 pKa = 10.75 DD561 pKa = 2.8 IFSFDD566 pKa = 3.83 AEE568 pKa = 4.43 SGHH571 pKa = 7.46 DD572 pKa = 3.86 IIRR575 pKa = 11.84 DD576 pKa = 3.81 FTPGEE581 pKa = 3.99 DD582 pKa = 4.03 KK583 pKa = 10.79 IALVADD589 pKa = 3.32 NWDD592 pKa = 3.79 ALQSQIDD599 pKa = 3.79 TSNANVPVITLNDD612 pKa = 3.65 GSTVAVTLSNGQGLSEE628 pKa = 4.06 NDD630 pKa = 3.86 FYY632 pKa = 12.11 YY633 pKa = 10.29 MGANTALVFGHH644 pKa = 6.96 TGTEE648 pKa = 4.38 GNDD651 pKa = 3.58 TIYY654 pKa = 11.28 DD655 pKa = 3.71 DD656 pKa = 3.92 TSIVALRR663 pKa = 11.84 GLSGDD668 pKa = 3.75 DD669 pKa = 3.95 TIHH672 pKa = 7.53 CEE674 pKa = 3.51 EE675 pKa = 5.49 GYY677 pKa = 10.85 NSGVFGDD684 pKa = 4.98 DD685 pKa = 3.84 GNDD688 pKa = 3.2 TIYY691 pKa = 11.22 ASNCSSGGFSGGAGDD706 pKa = 4.82 DD707 pKa = 3.85 RR708 pKa = 11.84 IFAGSAGGVFFGDD721 pKa = 3.75 AGDD724 pKa = 4.15 DD725 pKa = 3.65 SLYY728 pKa = 11.06 GGSGEE733 pKa = 4.92 DD734 pKa = 4.18 DD735 pKa = 5.18 FYY737 pKa = 11.5 FQDD740 pKa = 3.95 GFGHH744 pKa = 5.5 DD745 pKa = 3.93 TIYY748 pKa = 11.16 GFAASTSMVANVYY761 pKa = 9.3 EE762 pKa = 4.62 PKK764 pKa = 10.75 DD765 pKa = 3.67 KK766 pKa = 10.71 IALQGVSGLSDD777 pKa = 3.41 WEE779 pKa = 4.15 AVSNQITYY787 pKa = 10.18 TDD789 pKa = 4.58 LPDD792 pKa = 3.78 SGYY795 pKa = 10.88 CAIINDD801 pKa = 3.75 GHH803 pKa = 7.17 GNEE806 pKa = 4.32 IDD808 pKa = 3.36 IMLATGTQLVEE819 pKa = 3.93 SDD821 pKa = 4.72 FIFAA825 pKa = 5.1
Molecular weight: 87.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.666
IPC2_protein 3.694
IPC_protein 3.757
Toseland 3.516
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.427
Solomon 3.77
Lehninger 3.732
Nozaki 3.872
DTASelect 4.19
Thurlkill 3.579
EMBOSS 3.732
Sillero 3.884
Patrickios 1.214
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|A0A506U755|A0A506U755_9RHIZ Dephospho-CoA kinase OS=Martelella sp. BGMRC2036 OX=2590451 GN=coaE PE=3 SV=1
MM1 pKa = 6.51 STKK4 pKa = 9.4 RR5 pKa = 11.84 TYY7 pKa = 10.43 QPSKK11 pKa = 9.73 LVRR14 pKa = 11.84 KK15 pKa = 9.15 RR16 pKa = 11.84 RR17 pKa = 11.84 HH18 pKa = 4.42 GFRR21 pKa = 11.84 ARR23 pKa = 11.84 MATKK27 pKa = 10.27 GGRR30 pKa = 11.84 AVLAARR36 pKa = 11.84 RR37 pKa = 11.84 TRR39 pKa = 11.84 GRR41 pKa = 11.84 KK42 pKa = 9.13 RR43 pKa = 11.84 LSAA46 pKa = 4.03
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4394
0
4394
1450704
24
4640
330.2
35.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.964 ± 0.045
0.825 ± 0.011
5.942 ± 0.035
5.609 ± 0.034
4.064 ± 0.025
8.496 ± 0.068
1.974 ± 0.019
5.832 ± 0.025
3.549 ± 0.027
9.997 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.739 ± 0.019
2.906 ± 0.022
4.822 ± 0.031
3.01 ± 0.021
6.304 ± 0.043
5.842 ± 0.041
5.449 ± 0.034
7.058 ± 0.034
1.222 ± 0.017
2.397 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here