Mycoplasma mucosicanis
Average proteome isoelectric point is 7.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 686 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A507SQ54|A0A507SQ54_9MOLU F0F1 ATP synthase subunit beta OS=Mycoplasma mucosicanis OX=458208 GN=E1I18_02405 PE=3 SV=1
MM1 pKa = 7.85 LLRR4 pKa = 11.84 NEE6 pKa = 4.08 LKK8 pKa = 10.4 EE9 pKa = 4.04 YY10 pKa = 10.31 QDD12 pKa = 3.92 QINVLNMKK20 pKa = 9.93 DD21 pKa = 3.53 KK22 pKa = 9.94 KK23 pKa = 9.54 TEE25 pKa = 4.03 DD26 pKa = 3.21 EE27 pKa = 4.28 VQEE30 pKa = 4.22 LEE32 pKa = 4.22 NIIKK36 pKa = 10.26 IKK38 pKa = 10.67 EE39 pKa = 4.01 DD40 pKa = 3.29 LQDD43 pKa = 3.49 YY44 pKa = 11.1 LNILEE49 pKa = 5.06 IYY51 pKa = 10.66 SEE53 pKa = 4.3 FKK55 pKa = 10.5 PVQTQDD61 pKa = 3.07 ALDD64 pKa = 3.43 WDD66 pKa = 4.22 KK67 pKa = 11.28 EE68 pKa = 4.23 LKK70 pKa = 10.57 EE71 pKa = 4.67 FEE73 pKa = 4.59 NNPNALHH80 pKa = 5.79 QLNDD84 pKa = 3.64 EE85 pKa = 4.47 VKK87 pKa = 10.19 SFQDD91 pKa = 4.24 EE92 pKa = 3.92 IDD94 pKa = 3.93 ALNQKK99 pKa = 7.37 TDD101 pKa = 3.51 KK102 pKa = 11.01 SDD104 pKa = 3.52 EE105 pKa = 4.48 DD106 pKa = 3.88 NEE108 pKa = 4.16 QLKK111 pKa = 9.47 TIQEE115 pKa = 4.16 AKK117 pKa = 10.62 SNLEE121 pKa = 3.73 EE122 pKa = 4.34 FIIALEE128 pKa = 4.24 VYY130 pKa = 10.32 KK131 pKa = 10.43 DD132 pKa = 3.76 YY133 pKa = 11.54 KK134 pKa = 11.13 NSLEE138 pKa = 4.11 TDD140 pKa = 3.19 SDD142 pKa = 4.15 RR143 pKa = 11.84 DD144 pKa = 3.63 WQSLLDD150 pKa = 4.22 SIKK153 pKa = 10.68 ASQQAIEE160 pKa = 5.13 EE161 pKa = 4.32 LTQDD165 pKa = 3.05 IAKK168 pKa = 10.32 LKK170 pKa = 10.26 QQLQQQ175 pKa = 3.16
Molecular weight: 20.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.452
IPC2_protein 4.228
IPC_protein 4.19
Toseland 3.999
ProMoST 4.304
Dawson 4.139
Bjellqvist 4.291
Wikipedia 4.024
Rodwell 4.012
Grimsley 3.91
Solomon 4.139
Lehninger 4.088
Nozaki 4.24
DTASelect 4.418
Thurlkill 4.024
EMBOSS 4.037
Sillero 4.291
Patrickios 3.999
IPC_peptide 4.139
IPC2_peptide 4.279
IPC2.peptide.svr19 4.218
Protein with the highest isoelectric point:
>tr|A0A507SQM3|A0A507SQM3_9MOLU Elongation factor Ts OS=Mycoplasma mucosicanis OX=458208 GN=tsf PE=3 SV=1
MM1 pKa = 7.56 AHH3 pKa = 6.1 TKK5 pKa = 10.67 AGGSTRR11 pKa = 11.84 NGRR14 pKa = 11.84 DD15 pKa = 2.93 SRR17 pKa = 11.84 GQRR20 pKa = 11.84 LGIKK24 pKa = 10.22 LGDD27 pKa = 3.89 GQFCTAGSIIFRR39 pKa = 11.84 QRR41 pKa = 11.84 GTKK44 pKa = 9.65 IFPGTNVGIGKK55 pKa = 9.82 DD56 pKa = 3.4 DD57 pKa = 3.48 TLYY60 pKa = 11.6 ALINGYY66 pKa = 9.67 VKK68 pKa = 10.61 FEE70 pKa = 3.73 KK71 pKa = 10.27 RR72 pKa = 11.84 RR73 pKa = 11.84 NRR75 pKa = 11.84 TYY77 pKa = 11.25 ASVYY81 pKa = 6.31 EE82 pKa = 3.95 QRR84 pKa = 11.84 IVVSKK89 pKa = 11.39 NNN91 pKa = 3.37
Molecular weight: 10.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.897
IPC_protein 10.657
Toseland 10.76
ProMoST 10.452
Dawson 10.862
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 11.155
Grimsley 10.921
Solomon 10.965
Lehninger 10.935
Nozaki 10.745
DTASelect 10.57
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 10.906
IPC_peptide 10.979
IPC2_peptide 9.487
IPC2.peptide.svr19 8.431
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
686
0
686
257547
30
3675
375.4
42.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.708 ± 0.084
0.583 ± 0.026
5.814 ± 0.091
6.622 ± 0.096
5.235 ± 0.099
4.671 ± 0.086
1.68 ± 0.033
8.967 ± 0.109
10.082 ± 0.116
9.399 ± 0.085
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.668 ± 0.048
7.342 ± 0.103
2.756 ± 0.055
3.816 ± 0.058
3.18 ± 0.055
6.819 ± 0.064
5.072 ± 0.051
5.707 ± 0.078
0.925 ± 0.031
3.954 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here