Microbacterium sp. Root180

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3036 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8LNY5|A0A0Q8LNY5_9MICO Elongation factor Tu OS=Microbacterium sp. Root180 OX=1736483 GN=tuf PE=3 SV=1
MM1 pKa = 7.39LAASALALVACAGPSVPPDD20 pKa = 3.14VDD22 pKa = 4.37FDD24 pKa = 4.47DD25 pKa = 5.14PLPGAALAAAVDD37 pKa = 4.12DD38 pKa = 4.27AVTAVGVSDD47 pKa = 3.72GDD49 pKa = 3.68LWPSCWSDD57 pKa = 4.42DD58 pKa = 4.12DD59 pKa = 4.9ALYY62 pKa = 10.64AANGDD67 pKa = 3.64GAGFGNEE74 pKa = 3.92FFDD77 pKa = 3.86IAVNRR82 pKa = 11.84ITGSPTAEE90 pKa = 4.63DD91 pKa = 3.74GLEE94 pKa = 4.13GEE96 pKa = 4.72VLAGGDD102 pKa = 4.18AIGQVWSGEE111 pKa = 3.95GFTRR115 pKa = 11.84KK116 pKa = 7.75PTGMLCTGGSLYY128 pKa = 10.93LAVQDD133 pKa = 5.01LRR135 pKa = 11.84LDD137 pKa = 3.78FNEE140 pKa = 4.7APAATIARR148 pKa = 11.84SDD150 pKa = 3.53DD151 pKa = 4.41HH152 pKa = 6.61GATWTWDD159 pKa = 3.09TSAPMFDD166 pKa = 4.46DD167 pKa = 5.44GVFTTVWFADD177 pKa = 3.54YY178 pKa = 10.77GKK180 pKa = 9.96EE181 pKa = 4.26GEE183 pKa = 4.49HH184 pKa = 6.95APDD187 pKa = 4.03PGYY190 pKa = 11.18AYY192 pKa = 10.31AYY194 pKa = 10.15GLDD197 pKa = 3.99GNWRR201 pKa = 11.84DD202 pKa = 4.13SFDD205 pKa = 5.09DD206 pKa = 3.75SAPDD210 pKa = 3.55PTEE213 pKa = 4.23LFLARR218 pKa = 11.84VPVDD222 pKa = 3.72SVQDD226 pKa = 3.4ASTWEE231 pKa = 4.74FYY233 pKa = 10.94AGEE236 pKa = 4.58PGDD239 pKa = 6.0AEE241 pKa = 4.37PTWTGDD247 pKa = 2.85ISAKK251 pKa = 10.38RR252 pKa = 11.84PVLVDD257 pKa = 3.21EE258 pKa = 4.88RR259 pKa = 11.84VVHH262 pKa = 6.48PGDD265 pKa = 3.56GAGEE269 pKa = 4.49DD270 pKa = 4.13GQTVGAQNLTVISQGGVTYY289 pKa = 10.53LPKK292 pKa = 10.15LDD294 pKa = 3.68RR295 pKa = 11.84YY296 pKa = 10.97VYY298 pKa = 9.7TSWTEE303 pKa = 3.73LTFEE307 pKa = 4.9FYY309 pKa = 10.64EE310 pKa = 5.07SPTPWGPWTLFLSEE324 pKa = 5.46DD325 pKa = 3.68FGPYY329 pKa = 9.29PWTDD333 pKa = 3.04EE334 pKa = 4.22RR335 pKa = 11.84FGGYY339 pKa = 7.8GTTIPSKK346 pKa = 10.55FLSDD350 pKa = 4.47DD351 pKa = 3.58NRR353 pKa = 11.84SMWVQSNVCPCAPAGMSSYY372 pKa = 10.45WFGLRR377 pKa = 11.84PLYY380 pKa = 9.77LAPVDD385 pKa = 3.7

Molecular weight: 41.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8LKR9|A0A0Q8LKR9_9MICO Cytidyltransferase OS=Microbacterium sp. Root180 OX=1736483 GN=ASD93_14290 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.09RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.71GRR40 pKa = 11.84TEE42 pKa = 4.13LSAA45 pKa = 4.86

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3036

0

3036

1003784

38

3110

330.6

35.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.769 ± 0.065

0.472 ± 0.009

6.407 ± 0.037

5.734 ± 0.041

3.208 ± 0.03

9.007 ± 0.048

1.939 ± 0.023

4.544 ± 0.031

1.803 ± 0.033

9.973 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.762 ± 0.02

1.831 ± 0.027

5.5 ± 0.029

2.639 ± 0.024

7.256 ± 0.052

5.46 ± 0.033

5.958 ± 0.051

9.122 ± 0.043

1.617 ± 0.021

1.998 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski