Amino acid dipepetide frequency for Microbacterium sp. Root180

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.355AlaAla: 21.355 ± 0.236	0.67AlaCys: 0.67 ± 0.026	8.753AlaAsp: 8.753 ± 0.11	8.161AlaGlu: 8.161 ± 0.112	4.108AlaPhe: 4.108 ± 0.073	12.433AlaGly: 12.433 ± 0.142	2.463AlaHis: 2.463 ± 0.061	6.209AlaIle: 6.209 ± 0.084	2.592AlaLys: 2.592 ± 0.05	14.194AlaLeu: 14.194 ± 0.16	2.74AlaMet: 2.74 ± 0.058	2.234AlaAsn: 2.234 ± 0.059	6.893AlaPro: 6.893 ± 0.104	3.741AlaGln: 3.741 ± 0.073	9.373AlaArg: 9.373 ± 0.129	7.656AlaSer: 7.656 ± 0.099	7.487AlaThr: 7.487 ± 0.101	11.987AlaVal: 11.987 ± 0.139	2.059AlaTrp: 2.059 ± 0.048	2.588AlaTyr: 2.588 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.665CysAla: 0.665 ± 0.028	0.047CysCys: 0.047 ± 0.007	0.307CysAsp: 0.307 ± 0.017	0.244CysGlu: 0.244 ± 0.017	0.159CysPhe: 0.159 ± 0.013	0.495CysGly: 0.495 ± 0.025	0.111CysHis: 0.111 ± 0.011	0.177CysIle: 0.177 ± 0.012	0.061CysLys: 0.061 ± 0.009	0.409CysLeu: 0.409 ± 0.018	0.068CysMet: 0.068 ± 0.01	0.099CysAsn: 0.099 ± 0.011	0.279CysPro: 0.279 ± 0.017	0.12CysGln: 0.12 ± 0.011	0.321CysArg: 0.321 ± 0.019	0.276CysSer: 0.276 ± 0.016	0.317CysThr: 0.317 ± 0.02	0.398CysVal: 0.398 ± 0.02	0.075CysTrp: 0.075 ± 0.009	0.094CysTyr: 0.094 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	9.693AspAla: 9.693 ± 0.121	0.251AspCys: 0.251 ± 0.016	4.589AspAsp: 4.589 ± 0.089	4.626AspGlu: 4.626 ± 0.074	1.769AspPhe: 1.769 ± 0.044	6.489AspGly: 6.489 ± 0.099	1.207AspHis: 1.207 ± 0.038	2.535AspIle: 2.535 ± 0.051	0.989AspLys: 0.989 ± 0.033	6.311AspLeu: 6.311 ± 0.089	0.885AspMet: 0.885 ± 0.029	0.893AspAsn: 0.893 ± 0.029	4.451AspPro: 4.451 ± 0.083	1.442AspGln: 1.442 ± 0.039	4.637AspArg: 4.637 ± 0.079	2.579AspSer: 2.579 ± 0.054	2.79AspThr: 2.79 ± 0.061	5.706AspVal: 5.706 ± 0.089	0.975AspTrp: 0.975 ± 0.032	1.254AspTyr: 1.254 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	7.435GluAla: 7.435 ± 0.099	0.22GluCys: 0.22 ± 0.016	2.933GluAsp: 2.933 ± 0.057	3.143GluGlu: 3.143 ± 0.054	1.866GluPhe: 1.866 ± 0.047	4.325GluGly: 4.325 ± 0.069	1.346GluHis: 1.346 ± 0.04	2.734GluIle: 2.734 ± 0.059	1.273GluLys: 1.273 ± 0.039	6.535GluLeu: 6.535 ± 0.094	0.848GluMet: 0.848 ± 0.032	1.203GluAsn: 1.203 ± 0.04	3.191GluPro: 3.191 ± 0.062	1.94GluGln: 1.94 ± 0.051	5.226GluArg: 5.226 ± 0.094	2.987GluSer: 2.987 ± 0.046	3.071GluThr: 3.071 ± 0.058	4.83GluVal: 4.83 ± 0.078	1.009GluTrp: 1.009 ± 0.031	1.224GluTyr: 1.224 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	4.618PheAla: 4.618 ± 0.077	0.144PheCys: 0.144 ± 0.014	2.406PheAsp: 2.406 ± 0.051	1.897PheGlu: 1.897 ± 0.046	1.167PhePhe: 1.167 ± 0.039	3.383PheGly: 3.383 ± 0.064	0.561PheHis: 0.561 ± 0.026	1.184PheIle: 1.184 ± 0.044	0.402PheLys: 0.402 ± 0.023	2.913PheLeu: 2.913 ± 0.062	0.426PheMet: 0.426 ± 0.019	0.642PheAsn: 0.642 ± 0.03	1.464PhePro: 1.464 ± 0.039	0.818PheGln: 0.818 ± 0.03	1.904PheArg: 1.904 ± 0.047	1.73PheSer: 1.73 ± 0.042	2.166PheThr: 2.166 ± 0.056	3.031PheVal: 3.031 ± 0.054	0.552PheTrp: 0.552 ± 0.028	0.675PheTyr: 0.675 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	11.028GlyAla: 11.028 ± 0.151	0.53GlyCys: 0.53 ± 0.026	5.43GlyAsp: 5.43 ± 0.077	5.15GlyGlu: 5.15 ± 0.075	3.392GlyPhe: 3.392 ± 0.063	7.932GlyGly: 7.932 ± 0.125	1.783GlyHis: 1.783 ± 0.05	4.937GlyIle: 4.937 ± 0.073	1.943GlyLys: 1.943 ± 0.055	8.774GlyLeu: 8.774 ± 0.1	1.994GlyMet: 1.994 ± 0.05	1.553GlyAsn: 1.553 ± 0.045	3.868GlyPro: 3.868 ± 0.066	2.323GlyGln: 2.323 ± 0.055	6.368GlyArg: 6.368 ± 0.09	5.208GlySer: 5.208 ± 0.087	5.589GlyThr: 5.589 ± 0.096	8.224GlyVal: 8.224 ± 0.099	1.796GlyTrp: 1.796 ± 0.048	2.241GlyTyr: 2.241 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.407HisAla: 2.407 ± 0.054	0.098HisCys: 0.098 ± 0.009	1.283HisAsp: 1.283 ± 0.04	1.124HisGlu: 1.124 ± 0.032	0.561HisPhe: 0.561 ± 0.024	1.934HisGly: 1.934 ± 0.054	0.544HisHis: 0.544 ± 0.025	0.7HisIle: 0.7 ± 0.031	0.269HisLys: 0.269 ± 0.016	1.917HisLeu: 1.917 ± 0.052	0.292HisMet: 0.292 ± 0.017	0.323HisAsn: 0.323 ± 0.016	1.544HisPro: 1.544 ± 0.046	0.435HisGln: 0.435 ± 0.022	1.519HisArg: 1.519 ± 0.046	0.922HisSer: 0.922 ± 0.029	0.975HisThr: 0.975 ± 0.032	1.804HisVal: 1.804 ± 0.048	0.31HisTrp: 0.31 ± 0.019	0.426HisTyr: 0.426 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.273IleAla: 7.273 ± 0.096	0.202IleCys: 0.202 ± 0.013	3.521IleAsp: 3.521 ± 0.069	2.907IleGlu: 2.907 ± 0.056	1.182IlePhe: 1.182 ± 0.05	4.736IleGly: 4.736 ± 0.081	0.741IleHis: 0.741 ± 0.029	1.715IleIle: 1.715 ± 0.053	0.695IleLys: 0.695 ± 0.028	3.8IleLeu: 3.8 ± 0.075	0.605IleMet: 0.605 ± 0.028	0.827IleAsn: 0.827 ± 0.03	2.606IlePro: 2.606 ± 0.051	0.941IleGln: 0.941 ± 0.033	2.626IleArg: 2.626 ± 0.057	2.269IleSer: 2.269 ± 0.048	2.609IleThr: 2.609 ± 0.055	4.912IleVal: 4.912 ± 0.075	0.509IleTrp: 0.509 ± 0.022	0.761IleTyr: 0.761 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	2.266LysAla: 2.266 ± 0.053	0.061LysCys: 0.061 ± 0.007	1.069LysAsp: 1.069 ± 0.035	0.892LysGlu: 0.892 ± 0.033	0.431LysPhe: 0.431 ± 0.022	1.506LysGly: 1.506 ± 0.048	0.378LysHis: 0.378 ± 0.02	0.827LysIle: 0.827 ± 0.035	0.658LysLys: 0.658 ± 0.03	1.684LysLeu: 1.684 ± 0.04	0.311LysMet: 0.311 ± 0.017	0.419LysAsn: 0.419 ± 0.021	1.101LysPro: 1.101 ± 0.035	0.58LysGln: 0.58 ± 0.022	1.439LysArg: 1.439 ± 0.042	1.024LysSer: 1.024 ± 0.037	1.231LysThr: 1.231 ± 0.031	1.52LysVal: 1.52 ± 0.046	0.224LysTrp: 0.224 ± 0.017	0.409LysTyr: 0.409 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	14.784LeuAla: 14.784 ± 0.144	0.488LeuCys: 0.488 ± 0.021	6.701LeuAsp: 6.701 ± 0.104	5.489LeuGlu: 5.489 ± 0.083	3.007LeuPhe: 3.007 ± 0.067	8.975LeuGly: 8.975 ± 0.108	1.763LeuHis: 1.763 ± 0.047	4.333LeuIle: 4.333 ± 0.087	1.52LeuLys: 1.52 ± 0.044	9.627LeuLeu: 9.627 ± 0.128	1.615LeuMet: 1.615 ± 0.044	1.643LeuAsn: 1.643 ± 0.049	5.374LeuPro: 5.374 ± 0.078	2.462LeuGln: 2.462 ± 0.052	7.411LeuArg: 7.411 ± 0.103	5.65LeuSer: 5.65 ± 0.08	6.204LeuThr: 6.204 ± 0.088	9.51LeuVal: 9.51 ± 0.133	1.446LeuTrp: 1.446 ± 0.036	1.732LeuTyr: 1.732 ± 0.043	0.001LeuXaa: 0.001 ± 0.001
Met	2.114MetAla: 2.114 ± 0.046	0.097MetCys: 0.097 ± 0.009	0.821MetAsp: 0.821 ± 0.03	0.617MetGlu: 0.617 ± 0.023	0.502MetPhe: 0.502 ± 0.022	1.255MetGly: 1.255 ± 0.038	0.331MetHis: 0.331 ± 0.02	0.817MetIle: 0.817 ± 0.027	0.362MetLys: 0.362 ± 0.021	1.859MetLeu: 1.859 ± 0.041	0.335MetMet: 0.335 ± 0.022	0.431MetAsn: 0.431 ± 0.019	1.231MetPro: 1.231 ± 0.039	0.51MetGln: 0.51 ± 0.023	1.365MetArg: 1.365 ± 0.04	1.413MetSer: 1.413 ± 0.04	1.721MetThr: 1.721 ± 0.04	1.316MetVal: 1.316 ± 0.036	0.236MetTrp: 0.236 ± 0.016	0.286MetTyr: 0.286 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.382AsnAla: 2.382 ± 0.052	0.104AsnCys: 0.104 ± 0.011	1.067AsnAsp: 1.067 ± 0.034	0.919AsnGlu: 0.919 ± 0.034	0.579AsnPhe: 0.579 ± 0.023	1.798AsnGly: 1.798 ± 0.055	0.332AsnHis: 0.332 ± 0.02	0.778AsnIle: 0.778 ± 0.031	0.338AsnLys: 0.338 ± 0.021	1.797AsnLeu: 1.797 ± 0.046	0.301AsnMet: 0.301 ± 0.019	0.413AsnAsn: 0.413 ± 0.024	1.521AsnPro: 1.521 ± 0.043	0.527AsnGln: 0.527 ± 0.028	1.144AsnArg: 1.144 ± 0.036	0.906AsnSer: 0.906 ± 0.031	1.079AsnThr: 1.079 ± 0.04	1.575AsnVal: 1.575 ± 0.042	0.298AsnTrp: 0.298 ± 0.02	0.451AsnTyr: 0.451 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	7.671ProAla: 7.671 ± 0.11	0.176ProCys: 0.176 ± 0.012	4.181ProAsp: 4.181 ± 0.066	3.871ProGlu: 3.871 ± 0.063	1.813ProPhe: 1.813 ± 0.037	5.125ProGly: 5.125 ± 0.076	1.114ProHis: 1.114 ± 0.031	2.246ProIle: 2.246 ± 0.044	0.922ProLys: 0.922 ± 0.037	4.905ProLeu: 4.905 ± 0.073	0.903ProMet: 0.903 ± 0.029	0.966ProAsn: 0.966 ± 0.032	2.5ProPro: 2.5 ± 0.063	1.552ProGln: 1.552 ± 0.037	3.484ProArg: 3.484 ± 0.067	3.105ProSer: 3.105 ± 0.058	3.396ProThr: 3.396 ± 0.075	5.024ProVal: 5.024 ± 0.071	0.949ProTrp: 0.949 ± 0.033	1.096ProTyr: 1.096 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.363GlnAla: 3.363 ± 0.056	0.101GlnCys: 0.101 ± 0.01	1.265GlnAsp: 1.265 ± 0.032	1.252GlnGlu: 1.252 ± 0.035	0.858GlnPhe: 0.858 ± 0.027	1.951GlnGly: 1.951 ± 0.046	0.593GlnHis: 0.593 ± 0.025	1.273GlnIle: 1.273 ± 0.038	0.549GlnLys: 0.549 ± 0.025	3.088GlnLeu: 3.088 ± 0.056	0.474GlnMet: 0.474 ± 0.022	0.577GlnAsn: 0.577 ± 0.022	1.437GlnPro: 1.437 ± 0.044	1.058GlnGln: 1.058 ± 0.044	2.361GlnArg: 2.361 ± 0.05	1.428GlnSer: 1.428 ± 0.046	1.488GlnThr: 1.488 ± 0.041	2.208GlnVal: 2.208 ± 0.053	0.515GlnTrp: 0.515 ± 0.021	0.554GlnTyr: 0.554 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	9.157ArgAla: 9.157 ± 0.129	0.294ArgCys: 0.294 ± 0.019	4.467ArgAsp: 4.467 ± 0.062	4.486ArgGlu: 4.486 ± 0.08	2.452ArgPhe: 2.452 ± 0.05	5.664ArgGly: 5.664 ± 0.089	1.617ArgHis: 1.617 ± 0.044	3.471ArgIle: 3.471 ± 0.059	1.281ArgLys: 1.281 ± 0.037	7.285ArgLeu: 7.285 ± 0.11	1.681ArgMet: 1.681 ± 0.041	1.254ArgAsn: 1.254 ± 0.033	3.724ArgPro: 3.724 ± 0.071	1.828ArgGln: 1.828 ± 0.049	6.656ArgArg: 6.656 ± 0.107	3.957ArgSer: 3.957 ± 0.061	4.193ArgThr: 4.193 ± 0.071	6.341ArgVal: 6.341 ± 0.095	1.219ArgTrp: 1.219 ± 0.038	1.535ArgTyr: 1.535 ± 0.044	0.001ArgXaa: 0.001 ± 0.001
Ser	7.234SerAla: 7.234 ± 0.101	0.234SerCys: 0.234 ± 0.017	3.317SerAsp: 3.317 ± 0.057	2.601SerGlu: 2.601 ± 0.048	1.925SerPhe: 1.925 ± 0.04	5.745SerGly: 5.745 ± 0.081	1.014SerHis: 1.014 ± 0.035	2.585SerIle: 2.585 ± 0.051	0.96SerLys: 0.96 ± 0.034	5.301SerLeu: 5.301 ± 0.083	1.083SerMet: 1.083 ± 0.032	0.987SerAsn: 0.987 ± 0.032	3.18SerPro: 3.18 ± 0.061	1.344SerGln: 1.344 ± 0.039	3.699SerArg: 3.699 ± 0.07	3.15SerSer: 3.15 ± 0.069	3.406SerThr: 3.406 ± 0.071	4.778SerVal: 4.778 ± 0.082	0.945SerTrp: 0.945 ± 0.033	1.108SerTyr: 1.108 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	7.789ThrAla: 7.789 ± 0.115	0.292ThrCys: 0.292 ± 0.017	3.689ThrAsp: 3.689 ± 0.064	2.942ThrGlu: 2.942 ± 0.057	1.866ThrPhe: 1.866 ± 0.046	5.773ThrGly: 5.773 ± 0.088	1.108ThrHis: 1.108 ± 0.038	2.867ThrIle: 2.867 ± 0.061	1.06ThrLys: 1.06 ± 0.033	5.962ThrLeu: 5.962 ± 0.092	0.967ThrMet: 0.967 ± 0.03	1.118ThrAsn: 1.118 ± 0.048	3.901ThrPro: 3.901 ± 0.082	1.439ThrGln: 1.439 ± 0.039	3.729ThrArg: 3.729 ± 0.06	3.251ThrSer: 3.251 ± 0.065	3.795ThrThr: 3.795 ± 0.084	5.875ThrVal: 5.875 ± 0.11	0.961ThrTrp: 0.961 ± 0.035	1.198ThrTyr: 1.198 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	12.253ValAla: 12.253 ± 0.139	0.518ValCys: 0.518 ± 0.025	5.942ValAsp: 5.942 ± 0.094	5.164ValGlu: 5.164 ± 0.089	3.051ValPhe: 3.051 ± 0.052	7.339ValGly: 7.339 ± 0.088	1.741ValHis: 1.741 ± 0.044	4.481ValIle: 4.481 ± 0.074	1.55ValLys: 1.55 ± 0.048	9.402ValLeu: 9.402 ± 0.103	1.433ValMet: 1.433 ± 0.039	1.829ValAsn: 1.829 ± 0.045	4.895ValPro: 4.895 ± 0.077	2.226ValGln: 2.226 ± 0.049	6.388ValArg: 6.388 ± 0.083	4.983ValSer: 4.983 ± 0.074	5.846ValThr: 5.846 ± 0.097	9.281ValVal: 9.281 ± 0.11	1.312ValTrp: 1.312 ± 0.04	1.586ValTyr: 1.586 ± 0.038	0.001ValXaa: 0.001 ± 0.001
Trp	1.714TrpAla: 1.714 ± 0.042	0.087TrpCys: 0.087 ± 0.009	0.919TrpAsp: 0.919 ± 0.032	0.733TrpGlu: 0.733 ± 0.027	0.661TrpPhe: 0.661 ± 0.029	1.238TrpGly: 1.238 ± 0.039	0.356TrpHis: 0.356 ± 0.019	0.827TrpIle: 0.827 ± 0.034	0.258TrpLys: 0.258 ± 0.015	1.805TrpLeu: 1.805 ± 0.049	0.393TrpMet: 0.393 ± 0.02	0.459TrpAsn: 0.459 ± 0.024	0.766TrpPro: 0.766 ± 0.031	0.582TrpGln: 0.582 ± 0.026	1.272TrpArg: 1.272 ± 0.036	1.044TrpSer: 1.044 ± 0.033	1.072TrpThr: 1.072 ± 0.038	1.245TrpVal: 1.245 ± 0.036	0.425TrpTrp: 0.425 ± 0.025	0.315TrpTyr: 0.315 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.493TyrAla: 2.493 ± 0.05	0.108TyrCys: 0.108 ± 0.012	1.416TyrAsp: 1.416 ± 0.039	1.121TyrGlu: 1.121 ± 0.038	0.733TyrPhe: 0.733 ± 0.027	1.969TyrGly: 1.969 ± 0.046	0.284TyrHis: 0.284 ± 0.018	0.743TyrIle: 0.743 ± 0.033	0.348TyrLys: 0.348 ± 0.017	2.163TyrLeu: 2.163 ± 0.057	0.266TyrMet: 0.266 ± 0.016	0.437TyrAsn: 0.437 ± 0.023	1.072TyrPro: 1.072 ± 0.033	0.526TyrGln: 0.526 ± 0.02	1.642TyrArg: 1.642 ± 0.037	1.06TyrSer: 1.06 ± 0.036	1.146TyrThr: 1.146 ± 0.033	1.657TyrVal: 1.657 ± 0.041	0.354TyrTrp: 0.354 ± 0.018	0.445TyrTyr: 0.445 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3036 proteins (1003784 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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