Stenotrophomonas panacihumi
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3403 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R0AWU8|A0A0R0AWU8_9GAMM D-alanine--D-alanine ligase OS=Stenotrophomonas panacihumi OX=676599 GN=ddl PE=3 SV=1
MM1 pKa = 7.75 SDD3 pKa = 3.08 ATATTYY9 pKa = 10.8 RR10 pKa = 11.84 NWMCVVCGFIYY21 pKa = 10.52 RR22 pKa = 11.84 EE23 pKa = 3.76 ADD25 pKa = 3.38 GLPEE29 pKa = 4.09 EE30 pKa = 5.64 GIAPGTRR37 pKa = 11.84 WEE39 pKa = 5.53 DD40 pKa = 3.7 IPDD43 pKa = 3.14 TWTCPDD49 pKa = 3.91 CGVTKK54 pKa = 10.72 DD55 pKa = 4.17 DD56 pKa = 4.16 FEE58 pKa = 4.44 MMEE61 pKa = 3.98 IDD63 pKa = 3.61
Molecular weight: 7.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.77
IPC_protein 3.694
Toseland 3.49
ProMoST 3.783
Dawson 3.694
Bjellqvist 3.923
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.414
Solomon 3.656
Lehninger 3.617
Nozaki 3.834
DTASelect 4.024
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.821
Patrickios 0.693
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|A0A0R0AZN3|A0A0R0AZN3_9GAMM GTPase Era OS=Stenotrophomonas panacihumi OX=676599 GN=era PE=3 SV=1
MM1 pKa = 6.94 QRR3 pKa = 11.84 HH4 pKa = 5.79 APSPSHH10 pKa = 6.93 SPDD13 pKa = 3.12 PRR15 pKa = 11.84 VLRR18 pKa = 11.84 AVRR21 pKa = 11.84 QVALAGLALVLVWPAARR38 pKa = 11.84 GYY40 pKa = 8.32 SQWLGWGPLWLLGMPMTAWWALYY63 pKa = 10.04 RR64 pKa = 11.84 FRR66 pKa = 11.84 LPRR69 pKa = 11.84 WRR71 pKa = 11.84 VRR73 pKa = 11.84 RR74 pKa = 11.84 VPGARR79 pKa = 11.84 AMRR82 pKa = 11.84 RR83 pKa = 11.84 GPQARR88 pKa = 11.84 RR89 pKa = 11.84 RR90 pKa = 11.84 TAPRR94 pKa = 11.84 RR95 pKa = 11.84 GIAGLLARR103 pKa = 11.84 AAA105 pKa = 3.94
Molecular weight: 11.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.392
IPC2_protein 11.067
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.828
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.144
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3403
0
3403
1150310
30
3972
338.0
36.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.286 ± 0.066
0.752 ± 0.013
5.804 ± 0.029
5.341 ± 0.038
3.36 ± 0.028
8.739 ± 0.051
2.242 ± 0.026
3.926 ± 0.03
2.62 ± 0.037
10.866 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.117 ± 0.02
2.47 ± 0.035
5.415 ± 0.038
4.034 ± 0.028
7.592 ± 0.048
4.998 ± 0.036
4.852 ± 0.04
7.528 ± 0.037
1.644 ± 0.021
2.412 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here