New Jersey polyomavirus-2013
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A024B5E8|A0A024B5E8_9POLY Small T antigen OS=New Jersey polyomavirus-2013 OX=1497391 PE=4 SV=1
MM1 pKa = 7.52 GGLLSSLVDD10 pKa = 4.08 MIVMATEE17 pKa = 4.39 LSAASGLTMEE27 pKa = 5.3 ALLTGEE33 pKa = 4.6 ALAALEE39 pKa = 4.41 TEE41 pKa = 4.83 VFSLMTVEE49 pKa = 4.22 GLSGIEE55 pKa = 4.47 ALAQLGWTAEE65 pKa = 4.19 QFSNMAFISTTFSNAIGYY83 pKa = 8.57 GVLFQTVSGISSLISAGIRR102 pKa = 11.84 LGTSVSSVNRR112 pKa = 11.84 HH113 pKa = 3.58 QTEE116 pKa = 4.11 QEE118 pKa = 3.9 LEE120 pKa = 4.31 TIFGKK125 pKa = 10.04 IAHH128 pKa = 7.44 LIHH131 pKa = 6.78 VNLAFHH137 pKa = 7.1 LNPFNWCEE145 pKa = 4.3 SIGGTVPTEE154 pKa = 4.06 FNFLSLDD161 pKa = 3.6 QLSKK165 pKa = 11.01 LGVVIEE171 pKa = 4.01 NGRR174 pKa = 11.84 WVIQTTPSFDD184 pKa = 3.41 PMFEE188 pKa = 3.97 SGILIEE194 pKa = 5.13 RR195 pKa = 11.84 YY196 pKa = 8.52 EE197 pKa = 4.33 PPGGAYY203 pKa = 9.28 QRR205 pKa = 11.84 ATPDD209 pKa = 2.54 WMLPLILRR217 pKa = 11.84 LNGASQEE224 pKa = 4.13 KK225 pKa = 9.44 TPVCPTKK232 pKa = 10.84
Molecular weight: 25.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.649
IPC2_protein 4.673
IPC_protein 4.546
Toseland 4.431
ProMoST 4.635
Dawson 4.469
Bjellqvist 4.622
Wikipedia 4.291
Rodwell 4.406
Grimsley 4.355
Solomon 4.469
Lehninger 4.418
Nozaki 4.584
DTASelect 4.635
Thurlkill 4.418
EMBOSS 4.317
Sillero 4.66
Patrickios 3.745
IPC_peptide 4.482
IPC2_peptide 4.66
IPC2.peptide.svr19 4.578
Protein with the highest isoelectric point:
>tr|A0A024B5E3|A0A024B5E3_9POLY Minor capsid protein VP2 OS=New Jersey polyomavirus-2013 OX=1497391 GN=VP2 PE=3 SV=1
MM1 pKa = 7.51 EE2 pKa = 5.14 KK3 pKa = 10.45 VLEE6 pKa = 4.4 KK7 pKa = 10.38 SDD9 pKa = 4.07 KK10 pKa = 10.99 EE11 pKa = 3.97 MLIEE15 pKa = 3.83 LLGIPRR21 pKa = 11.84 YY22 pKa = 10.05 AYY24 pKa = 10.75 GNFPIMKK31 pKa = 8.48 TAYY34 pKa = 9.07 KK35 pKa = 9.52 RR36 pKa = 11.84 ASKK39 pKa = 9.97 IYY41 pKa = 10.0 HH42 pKa = 6.58 PDD44 pKa = 3.14 KK45 pKa = 11.07 GGSSEE50 pKa = 5.93 KK51 pKa = 10.35 MMLLNSLWQKK61 pKa = 9.68 FQEE64 pKa = 4.35 GLIEE68 pKa = 4.05 VRR70 pKa = 11.84 DD71 pKa = 3.94 SEE73 pKa = 4.69 VCQVSFSDD81 pKa = 5.25 CYY83 pKa = 11.12 DD84 pKa = 3.38 SSLLKK89 pKa = 10.67 CCSPKK94 pKa = 10.18 VFHH97 pKa = 6.8 EE98 pKa = 4.25 LFLRR102 pKa = 11.84 SPQCLLKK109 pKa = 11.02 GPTSCSCITSCLYY122 pKa = 9.95 NQHH125 pKa = 6.21 RR126 pKa = 11.84 QIKK129 pKa = 9.16 LCGKK133 pKa = 9.4 KK134 pKa = 10.02 RR135 pKa = 11.84 SFLIPMAAPTSEE147 pKa = 4.72 RR148 pKa = 11.84 DD149 pKa = 3.59 MQAGAPQYY157 pKa = 11.37 LPMKK161 pKa = 10.05 SLTRR165 pKa = 11.84 EE166 pKa = 3.64 QIYY169 pKa = 8.24 TVMSPPSPPPLMKK182 pKa = 10.47 KK183 pKa = 8.82 MKK185 pKa = 10.08 PKK187 pKa = 10.63 VQDD190 pKa = 3.42 TTLSPLLPPQPLPPPLPPKK209 pKa = 9.99 KK210 pKa = 9.74 YY211 pKa = 8.83 PRR213 pKa = 11.84 RR214 pKa = 11.84 SVSLNSPRR222 pKa = 11.84 VRR224 pKa = 11.84 LHH226 pKa = 6.91 PEE228 pKa = 3.46 ALLQEE233 pKa = 4.98 EE234 pKa = 4.4 IQRR237 pKa = 11.84 LRR239 pKa = 11.84 EE240 pKa = 3.95 SLHH243 pKa = 4.73 QRR245 pKa = 11.84 EE246 pKa = 4.26 EE247 pKa = 4.18 EE248 pKa = 4.2 VLKK251 pKa = 10.65 IWMDD255 pKa = 4.58 LILTRR260 pKa = 11.84 KK261 pKa = 7.77 QALRR265 pKa = 11.84 AHH267 pKa = 6.16 HH268 pKa = 5.66 QNKK271 pKa = 9.12 KK272 pKa = 9.87 GKK274 pKa = 9.46 VLIVLLICLLVCLILSVTLYY294 pKa = 10.97 LAIKK298 pKa = 9.34 QQ299 pKa = 3.69
Molecular weight: 34.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.925
IPC2_protein 8.814
IPC_protein 8.682
Toseland 9.487
ProMoST 9.238
Dawson 9.75
Bjellqvist 9.516
Wikipedia 9.882
Rodwell 10.131
Grimsley 9.794
Solomon 9.78
Lehninger 9.75
Nozaki 9.75
DTASelect 9.428
Thurlkill 9.619
EMBOSS 9.926
Sillero 9.75
Patrickios 5.296
IPC_peptide 9.78
IPC2_peptide 8.317
IPC2.peptide.svr19 7.926
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2101
183
711
350.2
39.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.807 ± 0.546
2.713 ± 0.667
4.046 ± 0.841
6.901 ± 0.248
4.522 ± 0.879
5.997 ± 0.935
1.904 ± 0.137
5.045 ± 0.651
6.901 ± 1.071
10.9 ± 0.932
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.999 ± 0.309
3.808 ± 0.542
6.711 ± 1.101
4.522 ± 0.245
3.76 ± 0.345
8.425 ± 0.767
5.474 ± 0.668
5.474 ± 0.403
1.285 ± 0.308
2.808 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here