Torque teno Leptonychotes weddellii virus-2
Average proteome isoelectric point is 5.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z2RW16|A0A1Z2RW16_9VIRU ORF3 OS=Torque teno Leptonychotes weddellii virus-2 OX=2012677 PE=4 SV=1
MM1 pKa = 7.52 ALSNKK6 pKa = 8.91 YY7 pKa = 10.0 RR8 pKa = 11.84 LAPYY12 pKa = 10.42 SKK14 pKa = 10.86 GPLRR18 pKa = 11.84 LPTSFRR24 pKa = 11.84 EE25 pKa = 4.17 TSTQTDD31 pKa = 3.32 ASKK34 pKa = 10.92 KK35 pKa = 10.38 ALFEE39 pKa = 4.01 EE40 pKa = 4.47 LLALIEE46 pKa = 4.89 QDD48 pKa = 5.69 SQPCWCQRR56 pKa = 11.84 EE57 pKa = 4.06 SDD59 pKa = 4.31 LDD61 pKa = 4.01 LEE63 pKa = 4.71 SEE65 pKa = 4.33 TDD67 pKa = 3.42 SEE69 pKa = 4.79 RR70 pKa = 11.84 SIEE73 pKa = 3.97 FLGSSWEE80 pKa = 4.14 DD81 pKa = 3.38 EE82 pKa = 4.32 EE83 pKa = 5.44 NPGGEE88 pKa = 4.57 GPPLVPPEE96 pKa = 5.46 GGAQWPSSFRR106 pKa = 11.84 FF107 pKa = 3.43
Molecular weight: 12.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.495
IPC2_protein 4.38
IPC_protein 4.253
Toseland 4.101
ProMoST 4.355
Dawson 4.19
Bjellqvist 4.342
Wikipedia 4.05
Rodwell 4.101
Grimsley 4.024
Solomon 4.19
Lehninger 4.139
Nozaki 4.304
DTASelect 4.406
Thurlkill 4.113
EMBOSS 4.062
Sillero 4.355
Patrickios 3.897
IPC_peptide 4.19
IPC2_peptide 4.355
IPC2.peptide.svr19 4.276
Protein with the highest isoelectric point:
>tr|A0A1Z2RVZ1|A0A1Z2RVZ1_9VIRU ORF2 OS=Torque teno Leptonychotes weddellii virus-2 OX=2012677 PE=4 SV=1
MM1 pKa = 7.33 ARR3 pKa = 11.84 FWRR6 pKa = 11.84 RR7 pKa = 11.84 WRR9 pKa = 11.84 KK10 pKa = 9.17 RR11 pKa = 11.84 PRR13 pKa = 11.84 IWRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 YY19 pKa = 8.83 RR20 pKa = 11.84 SYY22 pKa = 11.13 RR23 pKa = 11.84 PFRR26 pKa = 11.84 RR27 pKa = 11.84 WSHH30 pKa = 5.41 RR31 pKa = 11.84 RR32 pKa = 11.84 WGRR35 pKa = 11.84 RR36 pKa = 11.84 HH37 pKa = 5.82 RR38 pKa = 11.84 RR39 pKa = 11.84 HH40 pKa = 5.44 FRR42 pKa = 11.84 HH43 pKa = 6.44 RR44 pKa = 11.84 YY45 pKa = 7.57 AAVRR49 pKa = 11.84 YY50 pKa = 8.91 CPSRR54 pKa = 11.84 RR55 pKa = 11.84 HH56 pKa = 5.63 KK57 pKa = 10.76 KK58 pKa = 10.09 LVVSGWEE65 pKa = 3.72 PLGNVCATTTVCSEE79 pKa = 3.93 AKK81 pKa = 9.45 PYY83 pKa = 10.49 KK84 pKa = 10.04 ILEE87 pKa = 4.55 CKK89 pKa = 9.99 EE90 pKa = 4.54 SEE92 pKa = 4.12 IQQVANQMNKK102 pKa = 9.88 DD103 pKa = 3.9 GEE105 pKa = 4.61 WNGSWGHH112 pKa = 5.88 HH113 pKa = 4.66 WFSFAGLLARR123 pKa = 11.84 SQYY126 pKa = 10.45 FFNTFSTDD134 pKa = 2.52 WRR136 pKa = 11.84 SYY138 pKa = 11.45 DD139 pKa = 3.47 YY140 pKa = 11.4 LQFLGGYY147 pKa = 8.17 VWLPRR152 pKa = 11.84 QMYY155 pKa = 9.2 LDD157 pKa = 2.99 WMFYY161 pKa = 10.2 IDD163 pKa = 5.95 YY164 pKa = 10.6 DD165 pKa = 4.03 LQDD168 pKa = 3.45 KK169 pKa = 8.54 VTGEE173 pKa = 3.96 KK174 pKa = 9.37 MDD176 pKa = 3.38 MYY178 pKa = 10.71 KK179 pKa = 10.55 NSKK182 pKa = 7.07 TWFHH186 pKa = 6.57 PAILLNRR193 pKa = 11.84 RR194 pKa = 11.84 GGIIVGSPHH203 pKa = 6.95 RR204 pKa = 11.84 FGIKK208 pKa = 9.3 TMFRR212 pKa = 11.84 RR213 pKa = 11.84 LKK215 pKa = 10.45 VKK217 pKa = 10.43 PPANWEE223 pKa = 4.01 GLYY226 pKa = 10.44 NLPDD230 pKa = 3.96 ALHH233 pKa = 6.68 YY234 pKa = 10.85 IFFHH238 pKa = 6.37 WVWTWIDD245 pKa = 3.77 LEE247 pKa = 4.21 QSFMDD252 pKa = 3.99 SDD254 pKa = 3.87 WVKK257 pKa = 10.56 HH258 pKa = 5.48 IRR260 pKa = 11.84 SGDD263 pKa = 3.18 IGSQKK268 pKa = 9.97 PCEE271 pKa = 4.1 DD272 pKa = 3.75 VPWFLGGIEE281 pKa = 4.35 TKK283 pKa = 10.57 QPTVQQGFDD292 pKa = 3.68 KK293 pKa = 10.62 RR294 pKa = 11.84 ASWVDD299 pKa = 3.32 RR300 pKa = 11.84 EE301 pKa = 4.47 KK302 pKa = 11.33 YY303 pKa = 9.63 CCKK306 pKa = 9.94 KK307 pKa = 10.23 QRR309 pKa = 11.84 NFSDD313 pKa = 3.33 PMDD316 pKa = 3.88 NYY318 pKa = 10.61 GRR320 pKa = 11.84 WGPFCPYY327 pKa = 10.3 VYY329 pKa = 10.84 GMPYY333 pKa = 9.18 NQSRR337 pKa = 11.84 SLWFKK342 pKa = 10.47 YY343 pKa = 10.42 KK344 pKa = 10.69 FFFKK348 pKa = 10.83 LSGDD352 pKa = 3.6 SVYY355 pKa = 10.58 RR356 pKa = 11.84 RR357 pKa = 11.84 PPTSEE362 pKa = 4.15 AEE364 pKa = 4.23 EE365 pKa = 4.37 LVPEE369 pKa = 5.41 APGQQQNGPKK379 pKa = 9.39 QQIPSSSILKK389 pKa = 10.36 RR390 pKa = 11.84 PLTPSDD396 pKa = 4.04 ILPGDD401 pKa = 4.43 LDD403 pKa = 3.83 SDD405 pKa = 4.26 GCIKK409 pKa = 10.38 EE410 pKa = 4.21 SPLRR414 pKa = 11.84 RR415 pKa = 11.84 IISSHH420 pKa = 5.37 RR421 pKa = 11.84 TGQPTLLVPKK431 pKa = 10.12 RR432 pKa = 11.84 VRR434 pKa = 11.84 FRR436 pKa = 11.84 LGKK439 pKa = 9.56 RR440 pKa = 11.84 DD441 pKa = 3.61 RR442 pKa = 11.84 QRR444 pKa = 11.84 KK445 pKa = 5.01 IHH447 pKa = 6.85 RR448 pKa = 11.84 ILRR451 pKa = 11.84 QLMGGRR457 pKa = 11.84 RR458 pKa = 11.84 EE459 pKa = 4.4 SGGGRR464 pKa = 11.84 PPPGPPRR471 pKa = 11.84 RR472 pKa = 11.84 RR473 pKa = 11.84 GPMALQLPLLAHH485 pKa = 6.31 LHH487 pKa = 6.26 ILPQQ491 pKa = 3.68
Molecular weight: 58.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 9.706
IPC_protein 10.379
Toseland 10.452
ProMoST 10.16
Dawson 10.613
Bjellqvist 10.321
Wikipedia 10.818
Rodwell 10.877
Grimsley 10.687
Solomon 10.672
Lehninger 10.628
Nozaki 10.452
DTASelect 10.306
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.54
Patrickios 10.511
IPC_peptide 10.672
IPC2_peptide 9.311
IPC2.peptide.svr19 8.566
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
779
73
491
194.8
22.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.979 ± 0.782
2.182 ± 0.649
5.52 ± 0.678
5.392 ± 1.935
5.006 ± 0.407
8.986 ± 2.194
2.696 ± 0.733
4.236 ± 0.531
5.648 ± 0.5
8.858 ± 2.767
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.439 ± 0.398
2.182 ± 0.441
6.932 ± 1.444
4.236 ± 0.912
8.858 ± 2.829
7.574 ± 1.655
3.979 ± 0.92
3.723 ± 0.798
3.723 ± 0.702
3.851 ± 0.628
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here