Streptococcus phage Javan389

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AEK2|A0A4D6AEK2_9CAUD SGNH_hydro domain-containing protein OS=Streptococcus phage Javan389 OX=2548143 GN=Javan389_0049 PE=4 SV=1
MM1 pKa = 7.47SVTKK5 pKa = 10.37EE6 pKa = 3.97DD7 pKa = 3.83VISALNLDD15 pKa = 4.02EE16 pKa = 6.56SDD18 pKa = 4.96DD19 pKa = 4.41LSLLPLYY26 pKa = 10.39LKK28 pKa = 10.44SANTYY33 pKa = 9.72VINAIGKK40 pKa = 8.8EE41 pKa = 3.8IEE43 pKa = 4.69GYY45 pKa = 9.77PFYY48 pKa = 10.82TLADD52 pKa = 3.74VEE54 pKa = 5.12SEE56 pKa = 3.74FDD58 pKa = 3.45TAVIALTGTYY68 pKa = 7.56YY69 pKa = 10.34TYY71 pKa = 10.74RR72 pKa = 11.84IAVTDD77 pKa = 3.73TQSYY81 pKa = 9.22QMDD84 pKa = 3.74YY85 pKa = 9.68TLNSIVGQLRR95 pKa = 11.84GLYY98 pKa = 10.07ASYY101 pKa = 10.93LEE103 pKa = 4.3EE104 pKa = 4.32VEE106 pKa = 4.64NGQAVSTVV114 pKa = 2.96

Molecular weight: 12.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AB36|A0A4D6AB36_9CAUD Transcriptional activator OS=Streptococcus phage Javan389 OX=2548143 GN=Javan389_0027 PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84LLVFKK7 pKa = 10.17EE8 pKa = 4.21VSLEE12 pKa = 4.13EE13 pKa = 3.72VWKK16 pKa = 10.24EE17 pKa = 3.45IKK19 pKa = 10.5GYY21 pKa = 8.16EE22 pKa = 4.11GKK24 pKa = 10.71YY25 pKa = 9.01EE26 pKa = 3.78VSNFGRR32 pKa = 11.84VRR34 pKa = 11.84SFDD37 pKa = 3.21KK38 pKa = 11.06VLYY41 pKa = 9.89LKK43 pKa = 10.73NSSFIHH49 pKa = 6.41KK50 pKa = 10.26GRR52 pKa = 11.84IIKK55 pKa = 9.46LRR57 pKa = 11.84PGKK60 pKa = 9.03TSPYY64 pKa = 9.92LLASLSSNGVRR75 pKa = 11.84KK76 pKa = 10.05VKK78 pKa = 10.19LVHH81 pKa = 6.54RR82 pKa = 11.84LVAEE86 pKa = 4.17AFIDD90 pKa = 4.12NPDD93 pKa = 3.86LKK95 pKa = 11.11PEE97 pKa = 4.11VNHH100 pKa = 6.47KK101 pKa = 10.99NGVKK105 pKa = 9.6TDD107 pKa = 3.6NKK109 pKa = 10.51ASNLEE114 pKa = 4.18WVTSSEE120 pKa = 4.19NKK122 pKa = 8.1QHH124 pKa = 6.77ALKK127 pKa = 9.51TLPRR131 pKa = 11.84KK132 pKa = 7.91TLTSTNKK139 pKa = 10.1SGFSGVSLHH148 pKa = 6.83KK149 pKa = 10.43STGKK153 pKa = 8.28WQASLTHH160 pKa = 6.22NGKK163 pKa = 10.07RR164 pKa = 11.84MYY166 pKa = 10.55VGIYY170 pKa = 9.69RR171 pKa = 11.84SPKK174 pKa = 8.08EE175 pKa = 3.57AHH177 pKa = 6.67KK178 pKa = 10.95ALLHH182 pKa = 6.21RR183 pKa = 11.84KK184 pKa = 9.03EE185 pKa = 4.32EE186 pKa = 4.38LYY188 pKa = 9.18KK189 pKa = 10.49TT190 pKa = 3.89

Molecular weight: 21.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11770

39

1496

222.1

25.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.89 ± 0.503

0.595 ± 0.15

6.525 ± 0.294

6.975 ± 0.543

4.01 ± 0.185

6.576 ± 0.549

1.563 ± 0.154

7.043 ± 0.325

8.921 ± 0.428

8.496 ± 0.378

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.455 ± 0.187

6.143 ± 0.317

2.829 ± 0.162

3.679 ± 0.217

3.509 ± 0.305

6.5 ± 0.377

6.27 ± 0.364

5.743 ± 0.189

1.351 ± 0.13

3.925 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski