Amino acid dipepetide frequency for Streptococcus phage Javan389

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.19AlaAla: 1.19 ± 0.454
0.17AlaCys: 0.17 ± 0.099
4.588AlaAsp: 4.588 ± 0.458
4.588AlaGlu: 4.588 ± 0.728
2.464AlaPhe: 2.464 ± 0.424
4.758AlaGly: 4.758 ± 1.033
0.595AlaHis: 0.595 ± 0.252
5.438AlaIle: 5.438 ± 0.791
7.477AlaLys: 7.477 ± 0.896
6.373AlaLeu: 6.373 ± 0.818
2.039AlaMet: 2.039 ± 0.47
4.673AlaAsn: 4.673 ± 0.659
1.275AlaPro: 1.275 ± 0.347
2.464AlaGln: 2.464 ± 0.511
2.549AlaArg: 2.549 ± 0.404
5.948AlaSer: 5.948 ± 0.988
3.909AlaThr: 3.909 ± 0.796
4.333AlaVal: 4.333 ± 0.712
0.935AlaTrp: 0.935 ± 0.354
3.144AlaTyr: 3.144 ± 0.501
0.0AlaXaa: 0.0 ± 0.0
Cys
0.17CysAla: 0.17 ± 0.11
0.0CysCys: 0.0 ± 0.0
0.51CysAsp: 0.51 ± 0.226
0.17CysGlu: 0.17 ± 0.112
0.085CysPhe: 0.085 ± 0.081
0.85CysGly: 0.85 ± 0.437
0.17CysHis: 0.17 ± 0.128
0.255CysIle: 0.255 ± 0.151
1.02CysLys: 1.02 ± 0.434
0.34CysLeu: 0.34 ± 0.162
0.17CysMet: 0.17 ± 0.119
0.085CysAsn: 0.085 ± 0.077
0.34CysPro: 0.34 ± 0.173
0.255CysGln: 0.255 ± 0.194
0.425CysArg: 0.425 ± 0.303
0.255CysSer: 0.255 ± 0.213
0.34CysThr: 0.34 ± 0.157
0.34CysVal: 0.34 ± 0.162
0.085CysTrp: 0.085 ± 0.084
0.085CysTyr: 0.085 ± 0.09
0.0CysXaa: 0.0 ± 0.0
Asp
3.484AspAla: 3.484 ± 0.796
0.51AspCys: 0.51 ± 0.223
4.333AspAsp: 4.333 ± 0.777
4.843AspGlu: 4.843 ± 1.015
3.654AspPhe: 3.654 ± 0.411
4.673AspGly: 4.673 ± 0.653
0.765AspHis: 0.765 ± 0.263
5.098AspIle: 5.098 ± 0.846
5.268AspLys: 5.268 ± 0.741
5.438AspLeu: 5.438 ± 0.652
1.699AspMet: 1.699 ± 0.275
2.974AspAsn: 2.974 ± 0.529
2.209AspPro: 2.209 ± 0.467
1.444AspGln: 1.444 ± 0.383
2.379AspArg: 2.379 ± 0.538
4.079AspSer: 4.079 ± 0.511
3.909AspThr: 3.909 ± 0.7
4.503AspVal: 4.503 ± 0.636
0.85AspTrp: 0.85 ± 0.252
3.144AspTyr: 3.144 ± 0.474
0.0AspXaa: 0.0 ± 0.0
Glu
5.013GluAla: 5.013 ± 0.751
0.51GluCys: 0.51 ± 0.242
3.229GluAsp: 3.229 ± 0.656
5.863GluGlu: 5.863 ± 0.925
2.889GluPhe: 2.889 ± 0.598
3.484GluGly: 3.484 ± 0.484
0.85GluHis: 0.85 ± 0.303
5.693GluIle: 5.693 ± 0.867
5.098GluLys: 5.098 ± 0.96
8.497GluLeu: 8.497 ± 0.986
2.209GluMet: 2.209 ± 0.448
3.569GluAsn: 3.569 ± 0.441
1.614GluPro: 1.614 ± 0.313
2.634GluGln: 2.634 ± 0.462
3.059GluArg: 3.059 ± 0.721
2.974GluSer: 2.974 ± 0.531
4.333GluThr: 4.333 ± 0.638
4.163GluVal: 4.163 ± 0.648
0.935GluTrp: 0.935 ± 0.28
3.144GluTyr: 3.144 ± 0.474
0.0GluXaa: 0.0 ± 0.0
Phe
2.379PheAla: 2.379 ± 0.524
0.34PheCys: 0.34 ± 0.149
4.079PheAsp: 4.079 ± 0.461
2.209PheGlu: 2.209 ± 0.375
1.444PhePhe: 1.444 ± 0.33
3.654PheGly: 3.654 ± 0.651
0.51PheHis: 0.51 ± 0.191
2.719PheIle: 2.719 ± 0.48
3.144PheLys: 3.144 ± 0.551
3.144PheLeu: 3.144 ± 0.531
0.85PheMet: 0.85 ± 0.244
2.464PheAsn: 2.464 ± 0.526
0.425PhePro: 0.425 ± 0.166
0.68PheGln: 0.68 ± 0.23
1.36PheArg: 1.36 ± 0.367
2.889PheSer: 2.889 ± 0.615
2.719PheThr: 2.719 ± 0.489
2.294PheVal: 2.294 ± 0.33
0.68PheTrp: 0.68 ± 0.244
2.124PheTyr: 2.124 ± 0.417
0.0PheXaa: 0.0 ± 0.0
Gly
4.079GlyAla: 4.079 ± 1.107
0.255GlyCys: 0.255 ± 0.198
3.654GlyAsp: 3.654 ± 0.553
2.634GlyGlu: 2.634 ± 0.394
3.569GlyPhe: 3.569 ± 0.445
4.418GlyGly: 4.418 ± 0.942
1.275GlyHis: 1.275 ± 0.366
5.438GlyIle: 5.438 ± 0.977
7.052GlyLys: 7.052 ± 0.787
6.882GlyLeu: 6.882 ± 0.761
1.869GlyMet: 1.869 ± 0.397
3.909GlyAsn: 3.909 ± 0.917
0.51GlyPro: 0.51 ± 0.183
1.784GlyGln: 1.784 ± 0.417
2.294GlyArg: 2.294 ± 0.483
3.994GlySer: 3.994 ± 0.867
4.079GlyThr: 4.079 ± 0.735
3.824GlyVal: 3.824 ± 0.662
1.02GlyTrp: 1.02 ± 0.317
3.229GlyTyr: 3.229 ± 0.504
0.0GlyXaa: 0.0 ± 0.0
His
1.36HisAla: 1.36 ± 0.372
0.085HisCys: 0.085 ± 0.094
1.529HisAsp: 1.529 ± 0.451
1.02HisGlu: 1.02 ± 0.283
0.68HisPhe: 0.68 ± 0.281
0.85HisGly: 0.85 ± 0.311
0.255HisHis: 0.255 ± 0.145
1.19HisIle: 1.19 ± 0.28
1.614HisLys: 1.614 ± 0.437
0.68HisLeu: 0.68 ± 0.248
0.34HisMet: 0.34 ± 0.142
0.595HisAsn: 0.595 ± 0.229
0.425HisPro: 0.425 ± 0.2
0.51HisGln: 0.51 ± 0.197
0.68HisArg: 0.68 ± 0.293
0.935HisSer: 0.935 ± 0.302
0.85HisThr: 0.85 ± 0.186
1.19HisVal: 1.19 ± 0.326
0.17HisTrp: 0.17 ± 0.135
0.68HisTyr: 0.68 ± 0.237
0.0HisXaa: 0.0 ± 0.0
Ile
5.438IleAla: 5.438 ± 0.781
0.425IleCys: 0.425 ± 0.183
5.268IleAsp: 5.268 ± 0.794
5.013IleGlu: 5.013 ± 0.828
2.124IlePhe: 2.124 ± 0.48
3.569IleGly: 3.569 ± 0.516
1.444IleHis: 1.444 ± 0.278
4.418IleIle: 4.418 ± 0.615
7.477IleLys: 7.477 ± 0.808
5.098IleLeu: 5.098 ± 0.648
1.529IleMet: 1.529 ± 0.362
4.928IleAsn: 4.928 ± 0.665
3.144IlePro: 3.144 ± 0.687
2.294IleGln: 2.294 ± 0.336
2.719IleArg: 2.719 ± 0.617
4.418IleSer: 4.418 ± 0.61
4.079IleThr: 4.079 ± 0.76
3.824IleVal: 3.824 ± 0.63
0.595IleTrp: 0.595 ± 0.187
2.634IleTyr: 2.634 ± 0.582
0.0IleXaa: 0.0 ± 0.0
Lys
7.137LysAla: 7.137 ± 0.758
0.595LysCys: 0.595 ± 0.331
5.013LysAsp: 5.013 ± 0.632
7.987LysGlu: 7.987 ± 1.172
3.484LysPhe: 3.484 ± 0.855
4.673LysGly: 4.673 ± 0.751
1.444LysHis: 1.444 ± 0.393
6.798LysIle: 6.798 ± 0.93
7.477LysLys: 7.477 ± 0.965
6.967LysLeu: 6.967 ± 0.723
1.954LysMet: 1.954 ± 0.397
5.353LysAsn: 5.353 ± 0.913
2.124LysPro: 2.124 ± 0.373
4.163LysGln: 4.163 ± 0.482
2.719LysArg: 2.719 ± 0.445
5.013LysSer: 5.013 ± 0.696
5.608LysThr: 5.608 ± 0.937
5.438LysVal: 5.438 ± 0.933
1.954LysTrp: 1.954 ± 0.374
3.314LysTyr: 3.314 ± 0.621
0.0LysXaa: 0.0 ± 0.0
Leu
6.628LeuAla: 6.628 ± 0.952
0.425LeuCys: 0.425 ± 0.209
5.948LeuAsp: 5.948 ± 0.753
5.693LeuGlu: 5.693 ± 1.089
2.294LeuPhe: 2.294 ± 0.412
4.673LeuGly: 4.673 ± 0.733
1.36LeuHis: 1.36 ± 0.454
4.503LeuIle: 4.503 ± 0.628
9.517LeuLys: 9.517 ± 0.881
6.882LeuLeu: 6.882 ± 1.16
1.869LeuMet: 1.869 ± 0.338
6.033LeuAsn: 6.033 ± 0.683
3.144LeuPro: 3.144 ± 0.719
3.229LeuGln: 3.229 ± 0.556
2.719LeuArg: 2.719 ± 0.577
5.948LeuSer: 5.948 ± 0.792
6.798LeuThr: 6.798 ± 0.789
4.503LeuVal: 4.503 ± 0.614
0.51LeuTrp: 0.51 ± 0.2
2.294LeuTyr: 2.294 ± 0.457
0.0LeuXaa: 0.0 ± 0.0
Met
1.529MetAla: 1.529 ± 0.382
0.085MetCys: 0.085 ± 0.08
0.68MetAsp: 0.68 ± 0.266
1.529MetGlu: 1.529 ± 0.367
1.19MetPhe: 1.19 ± 0.22
1.784MetGly: 1.784 ± 0.358
0.17MetHis: 0.17 ± 0.147
1.529MetIle: 1.529 ± 0.27
2.209MetLys: 2.209 ± 0.412
1.954MetLeu: 1.954 ± 0.445
0.765MetMet: 0.765 ± 0.271
2.379MetAsn: 2.379 ± 0.503
1.02MetPro: 1.02 ± 0.251
0.595MetGln: 0.595 ± 0.202
1.105MetArg: 1.105 ± 0.312
1.869MetSer: 1.869 ± 0.406
1.954MetThr: 1.954 ± 0.35
1.275MetVal: 1.275 ± 0.316
0.255MetTrp: 0.255 ± 0.167
0.68MetTyr: 0.68 ± 0.278
0.0MetXaa: 0.0 ± 0.0
Asn
6.373AsnAla: 6.373 ± 1.031
0.17AsnCys: 0.17 ± 0.137
4.079AsnAsp: 4.079 ± 0.584
3.229AsnGlu: 3.229 ± 0.599
2.294AsnPhe: 2.294 ± 0.466
6.033AsnGly: 6.033 ± 1.093
1.105AsnHis: 1.105 ± 0.4
4.079AsnIle: 4.079 ± 0.562
5.013AsnLys: 5.013 ± 0.645
3.739AsnLeu: 3.739 ± 0.526
1.36AsnMet: 1.36 ± 0.291
3.484AsnAsn: 3.484 ± 0.7
1.869AsnPro: 1.869 ± 0.457
3.144AsnGln: 3.144 ± 0.482
1.869AsnArg: 1.869 ± 0.374
4.588AsnSer: 4.588 ± 0.629
3.144AsnThr: 3.144 ± 0.557
2.634AsnVal: 2.634 ± 0.544
0.935AsnTrp: 0.935 ± 0.333
2.209AsnTyr: 2.209 ± 0.455
0.0AsnXaa: 0.0 ± 0.0
Pro
1.784ProAla: 1.784 ± 0.293
0.17ProCys: 0.17 ± 0.125
2.124ProAsp: 2.124 ± 0.469
2.379ProGlu: 2.379 ± 0.499
1.105ProPhe: 1.105 ± 0.352
1.02ProGly: 1.02 ± 0.31
0.255ProHis: 0.255 ± 0.129
2.124ProIle: 2.124 ± 0.363
2.719ProLys: 2.719 ± 0.472
2.039ProLeu: 2.039 ± 0.522
0.765ProMet: 0.765 ± 0.239
1.529ProAsn: 1.529 ± 0.257
0.68ProPro: 0.68 ± 0.246
1.699ProGln: 1.699 ± 0.434
1.105ProArg: 1.105 ± 0.38
1.869ProSer: 1.869 ± 0.377
2.039ProThr: 2.039 ± 0.413
1.784ProVal: 1.784 ± 0.528
0.34ProTrp: 0.34 ± 0.155
0.765ProTyr: 0.765 ± 0.287
0.0ProXaa: 0.0 ± 0.0
Gln
3.484GlnAla: 3.484 ± 0.579
0.255GlnCys: 0.255 ± 0.139
1.529GlnAsp: 1.529 ± 0.441
2.634GlnGlu: 2.634 ± 0.51
1.105GlnPhe: 1.105 ± 0.37
2.974GlnGly: 2.974 ± 0.447
0.85GlnHis: 0.85 ± 0.301
2.549GlnIle: 2.549 ± 0.493
2.974GlnLys: 2.974 ± 0.477
3.484GlnLeu: 3.484 ± 0.479
1.19GlnMet: 1.19 ± 0.292
1.954GlnAsn: 1.954 ± 0.324
1.19GlnPro: 1.19 ± 0.272
1.614GlnGln: 1.614 ± 0.461
0.935GlnArg: 0.935 ± 0.262
1.869GlnSer: 1.869 ± 0.343
1.954GlnThr: 1.954 ± 0.375
2.124GlnVal: 2.124 ± 0.534
0.595GlnTrp: 0.595 ± 0.234
1.529GlnTyr: 1.529 ± 0.292
0.0GlnXaa: 0.0 ± 0.0
Arg
1.529ArgAla: 1.529 ± 0.374
0.425ArgCys: 0.425 ± 0.222
2.634ArgAsp: 2.634 ± 0.534
2.804ArgGlu: 2.804 ± 0.57
1.444ArgPhe: 1.444 ± 0.412
1.699ArgGly: 1.699 ± 0.371
0.68ArgHis: 0.68 ± 0.275
2.804ArgIle: 2.804 ± 0.528
2.804ArgLys: 2.804 ± 0.544
3.654ArgLeu: 3.654 ± 0.59
0.85ArgMet: 0.85 ± 0.266
2.464ArgAsn: 2.464 ± 0.547
0.765ArgPro: 0.765 ± 0.395
1.529ArgGln: 1.529 ± 0.352
1.36ArgArg: 1.36 ± 0.365
1.614ArgSer: 1.614 ± 0.419
1.869ArgThr: 1.869 ± 0.436
1.954ArgVal: 1.954 ± 0.383
0.85ArgTrp: 0.85 ± 0.305
1.36ArgTyr: 1.36 ± 0.285
0.0ArgXaa: 0.0 ± 0.0
Ser
4.248SerAla: 4.248 ± 0.596
0.34SerCys: 0.34 ± 0.202
4.418SerAsp: 4.418 ± 0.657
4.928SerGlu: 4.928 ± 0.656
2.634SerPhe: 2.634 ± 0.592
5.183SerGly: 5.183 ± 0.816
1.19SerHis: 1.19 ± 0.438
4.843SerIle: 4.843 ± 0.64
5.778SerLys: 5.778 ± 1.019
5.098SerLeu: 5.098 ± 0.557
1.699SerMet: 1.699 ± 0.377
4.418SerAsn: 4.418 ± 0.788
1.954SerPro: 1.954 ± 0.478
1.869SerGln: 1.869 ± 0.332
2.124SerArg: 2.124 ± 0.556
4.163SerSer: 4.163 ± 0.747
3.569SerThr: 3.569 ± 0.708
3.569SerVal: 3.569 ± 0.543
0.85SerTrp: 0.85 ± 0.249
2.124SerTyr: 2.124 ± 0.496
0.0SerXaa: 0.0 ± 0.0
Thr
5.353ThrAla: 5.353 ± 0.769
0.085ThrCys: 0.085 ± 0.087
3.059ThrAsp: 3.059 ± 0.493
3.824ThrGlu: 3.824 ± 0.551
2.889ThrPhe: 2.889 ± 0.4
5.013ThrGly: 5.013 ± 0.873
1.105ThrHis: 1.105 ± 0.373
4.588ThrIle: 4.588 ± 0.741
4.673ThrLys: 4.673 ± 0.634
4.503ThrLeu: 4.503 ± 0.672
1.105ThrMet: 1.105 ± 0.211
3.909ThrAsn: 3.909 ± 0.69
1.444ThrPro: 1.444 ± 0.365
2.209ThrGln: 2.209 ± 0.326
2.039ThrArg: 2.039 ± 0.433
4.928ThrSer: 4.928 ± 0.82
4.588ThrThr: 4.588 ± 0.795
3.994ThrVal: 3.994 ± 0.578
0.935ThrTrp: 0.935 ± 0.36
2.464ThrTyr: 2.464 ± 0.505
0.0ThrXaa: 0.0 ± 0.0
Val
4.163ValAla: 4.163 ± 0.463
0.17ValCys: 0.17 ± 0.134
4.418ValAsp: 4.418 ± 0.843
4.758ValGlu: 4.758 ± 0.859
2.209ValPhe: 2.209 ± 0.313
3.909ValGly: 3.909 ± 0.712
0.765ValHis: 0.765 ± 0.42
3.229ValIle: 3.229 ± 0.573
4.079ValLys: 4.079 ± 0.65
4.333ValLeu: 4.333 ± 0.535
1.02ValMet: 1.02 ± 0.289
3.994ValAsn: 3.994 ± 0.537
1.869ValPro: 1.869 ± 0.61
2.294ValGln: 2.294 ± 0.489
1.444ValArg: 1.444 ± 0.412
4.248ValSer: 4.248 ± 0.666
4.163ValThr: 4.163 ± 0.55
3.314ValVal: 3.314 ± 0.542
0.51ValTrp: 0.51 ± 0.253
2.549ValTyr: 2.549 ± 0.458
0.0ValXaa: 0.0 ± 0.0
Trp
0.68TrpAla: 0.68 ± 0.263
0.085TrpCys: 0.085 ± 0.09
1.105TrpAsp: 1.105 ± 0.359
0.85TrpGlu: 0.85 ± 0.247
0.34TrpPhe: 0.34 ± 0.149
0.935TrpGly: 0.935 ± 0.298
0.255TrpHis: 0.255 ± 0.131
0.595TrpIle: 0.595 ± 0.186
1.444TrpLys: 1.444 ± 0.364
1.699TrpLeu: 1.699 ± 0.436
0.17TrpMet: 0.17 ± 0.123
0.935TrpAsn: 0.935 ± 0.248
0.51TrpPro: 0.51 ± 0.284
0.595TrpGln: 0.595 ± 0.225
0.51TrpArg: 0.51 ± 0.274
0.935TrpSer: 0.935 ± 0.392
0.68TrpThr: 0.68 ± 0.232
0.85TrpVal: 0.85 ± 0.255
0.085TrpTrp: 0.085 ± 0.079
0.255TrpTyr: 0.255 ± 0.176
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.889TyrAla: 2.889 ± 0.529
0.85TyrCys: 0.85 ± 0.276
3.059TyrAsp: 3.059 ± 0.742
2.804TyrGlu: 2.804 ± 0.43
2.209TyrPhe: 2.209 ± 0.443
1.614TyrGly: 1.614 ± 0.371
0.595TyrHis: 0.595 ± 0.266
2.549TyrIle: 2.549 ± 0.532
2.379TyrLys: 2.379 ± 0.466
4.163TyrLeu: 4.163 ± 0.588
1.02TyrMet: 1.02 ± 0.238
1.784TyrAsn: 1.784 ± 0.48
1.784TyrPro: 1.784 ± 0.411
1.784TyrGln: 1.784 ± 0.485
1.699TyrArg: 1.699 ± 0.348
2.464TyrSer: 2.464 ± 0.488
2.124TyrThr: 2.124 ± 0.549
1.529TyrVal: 1.529 ± 0.312
0.425TyrTrp: 0.425 ± 0.181
1.529TyrTyr: 1.529 ± 0.426
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (11770 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski