Achromobacter phage vB_AxyP_19-32_Axy10
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A514CU38|A0A514CU38_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyP_19-32_Axy10 OX=2591041 GN=Axy10_027 PE=4 SV=1
MM1 pKa = 7.2 YY2 pKa = 10.48 SWLDD6 pKa = 3.62 KK7 pKa = 10.76 IIEE10 pKa = 4.12 EE11 pKa = 3.93 QAAYY15 pKa = 11.03 YY16 pKa = 11.16 GMTPEE21 pKa = 4.12 KK22 pKa = 10.22 WEE24 pKa = 3.99 QLKK27 pKa = 10.01 EE28 pKa = 3.61 AAIQYY33 pKa = 9.58 EE34 pKa = 4.39 EE35 pKa = 4.56 EE36 pKa = 4.23 NPHH39 pKa = 5.02 VV40 pKa = 3.79
Molecular weight: 4.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.131
IPC2_protein 4.495
IPC_protein 4.253
Toseland 4.139
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.012
Rodwell 4.113
Grimsley 4.062
Solomon 4.164
Lehninger 4.113
Nozaki 4.304
DTASelect 4.317
Thurlkill 4.139
EMBOSS 4.037
Sillero 4.355
Patrickios 1.952
IPC_peptide 4.177
IPC2_peptide 4.355
IPC2.peptide.svr19 4.28
Protein with the highest isoelectric point:
>tr|A0A514CU26|A0A514CU26_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyP_19-32_Axy10 OX=2591041 GN=Axy10_037 PE=4 SV=1
MM1 pKa = 7.31 TRR3 pKa = 11.84 RR4 pKa = 11.84 IDD6 pKa = 3.17 HH7 pKa = 6.38 WNGNRR12 pKa = 11.84 NEE14 pKa = 4.3 AFYY17 pKa = 10.9 SSHH20 pKa = 6.06 PTRR23 pKa = 11.84 LQEE26 pKa = 4.85 IRR28 pKa = 11.84 DD29 pKa = 3.74 RR30 pKa = 11.84 TNNQIPFNWKK40 pKa = 7.06 TRR42 pKa = 11.84 KK43 pKa = 9.34 CVCCKK48 pKa = 10.14 RR49 pKa = 11.84 SRR51 pKa = 11.84 SLTQFNGDD59 pKa = 2.98 SATCKK64 pKa = 9.98 RR65 pKa = 11.84 CPPEE69 pKa = 3.73 KK70 pKa = 10.66
Molecular weight: 8.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.272
IPC2_protein 9.194
IPC_protein 9.37
Toseland 10.277
ProMoST 9.984
Dawson 10.379
Bjellqvist 10.072
Wikipedia 10.511
Rodwell 10.73
Grimsley 10.409
Solomon 10.452
Lehninger 10.438
Nozaki 10.394
DTASelect 10.014
Thurlkill 10.292
EMBOSS 10.657
Sillero 10.335
Patrickios 10.57
IPC_peptide 10.467
IPC2_peptide 9.458
IPC2.peptide.svr19 8.115
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
83
0
83
23004
40
3437
277.2
30.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.255 ± 0.635
0.696 ± 0.11
5.847 ± 0.132
6.508 ± 0.227
3.525 ± 0.151
6.894 ± 0.191
1.987 ± 0.185
5.382 ± 0.275
6.282 ± 0.226
8.081 ± 0.228
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.286 ± 0.119
4.804 ± 0.168
4.656 ± 0.234
4.704 ± 0.255
4.977 ± 0.182
5.56 ± 0.164
5.856 ± 0.255
6.877 ± 0.274
1.504 ± 0.209
3.321 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here