Anaerotruncus colihominis DSM 17241
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4421 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B0PC25|B0PC25_9FIRM NLPA lipoprotein OS=Anaerotruncus colihominis DSM 17241 OX=445972 GN=ANACOL_02334 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.34 KK3 pKa = 9.75 ILALTLALCMLFTSMVGCSSGSDD26 pKa = 3.09 TTAAGTGSNTSTEE39 pKa = 4.2 AGTTQPDD46 pKa = 3.37 TGDD49 pKa = 3.58 AEE51 pKa = 4.52 ASEE54 pKa = 4.96 EE55 pKa = 4.03 PTEE58 pKa = 4.3 TNDD61 pKa = 5.4 DD62 pKa = 3.74 PTADD66 pKa = 3.77 LQVSEE71 pKa = 5.06 DD72 pKa = 3.44 APTYY76 pKa = 10.91 VMVSKK81 pKa = 9.46 TLSDD85 pKa = 3.51 PVFIDD90 pKa = 3.14 MYY92 pKa = 9.96 IGFRR96 pKa = 11.84 EE97 pKa = 4.15 FCEE100 pKa = 4.53 SIGANCMYY108 pKa = 10.11 RR109 pKa = 11.84 GSDD112 pKa = 3.13 EE113 pKa = 3.91 PTAEE117 pKa = 4.26 KK118 pKa = 10.13 EE119 pKa = 4.01 IEE121 pKa = 4.57 IITQLMAQGVDD132 pKa = 3.35 GLAVIAADD140 pKa = 4.51 FDD142 pKa = 3.94 ALEE145 pKa = 4.38 PVLTQAMGQGIAVVTFDD162 pKa = 4.3 SAANPDD168 pKa = 3.35 SRR170 pKa = 11.84 QLHH173 pKa = 5.48 VEE175 pKa = 3.81 QASIDD180 pKa = 3.87 LVGRR184 pKa = 11.84 DD185 pKa = 3.59 QMKK188 pKa = 10.32 SALEE192 pKa = 4.13 IIGGPGAEE200 pKa = 3.96 GTVGILSAQPEE211 pKa = 4.68 SQLHH215 pKa = 6.44 ADD217 pKa = 3.47 WCNAMLKK224 pKa = 10.0 EE225 pKa = 4.53 VEE227 pKa = 4.79 DD228 pKa = 4.04 NPEE231 pKa = 3.79 DD232 pKa = 3.8 FANVTVLPIAYY243 pKa = 10.05 GDD245 pKa = 4.17 DD246 pKa = 4.29 LPDD249 pKa = 3.88 KK250 pKa = 10.4 STTEE254 pKa = 3.92 AQAMLQNYY262 pKa = 8.99 PDD264 pKa = 3.76 IDD266 pKa = 4.32 VIISPTTVGILSAAKK281 pKa = 9.83 VIQDD285 pKa = 3.67 MGSEE289 pKa = 4.24 CKK291 pKa = 9.13 VTGVGLPSEE300 pKa = 4.16 MAPYY304 pKa = 9.72 IEE306 pKa = 5.9 SGICYY311 pKa = 10.06 DD312 pKa = 3.82 CYY314 pKa = 11.06 LWNPYY319 pKa = 9.58 DD320 pKa = 3.58 QGYY323 pKa = 8.72 LAAASVNSISTGEE336 pKa = 4.09 STGAVGDD343 pKa = 3.94 TVTAGRR349 pKa = 11.84 LGEE352 pKa = 4.22 YY353 pKa = 9.03 TVEE356 pKa = 4.1 EE357 pKa = 5.03 YY358 pKa = 11.3 YY359 pKa = 11.04 DD360 pKa = 3.91 GGTQVLLGDD369 pKa = 4.81 PIRR372 pKa = 11.84 FTKK375 pKa = 10.33 EE376 pKa = 3.45 NIGEE380 pKa = 4.18 YY381 pKa = 10.36 KK382 pKa = 10.75 DD383 pKa = 4.21 LFF385 pKa = 4.8
Molecular weight: 40.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.745
IPC_protein 3.745
Toseland 3.541
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.643
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.05
Thurlkill 3.579
EMBOSS 3.656
Sillero 3.859
Patrickios 0.693
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.775
Protein with the highest isoelectric point:
>tr|B0P7H2|B0P7H2_9FIRM Uncharacterized protein OS=Anaerotruncus colihominis DSM 17241 OX=445972 GN=ANACOL_00703 PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.12 KK3 pKa = 9.97 FHH5 pKa = 5.45 VTRR8 pKa = 11.84 KK9 pKa = 7.37 TVLTIVGVTLPLTAAAILLPRR30 pKa = 11.84 LKK32 pKa = 10.11 RR33 pKa = 11.84 SRR35 pKa = 11.84 RR36 pKa = 11.84 ARR38 pKa = 11.84 SVRR41 pKa = 11.84 AA42 pKa = 3.42
Molecular weight: 4.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.038
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.501
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.237
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.083
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4421
0
4421
1126561
39
2434
254.8
28.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.958 ± 0.061
1.725 ± 0.02
5.623 ± 0.039
6.35 ± 0.04
4.03 ± 0.026
7.799 ± 0.041
1.772 ± 0.018
6.24 ± 0.043
4.781 ± 0.035
9.487 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.929 ± 0.02
3.402 ± 0.025
4.23 ± 0.031
3.545 ± 0.024
5.958 ± 0.043
5.674 ± 0.036
5.224 ± 0.029
6.895 ± 0.037
0.969 ± 0.015
3.408 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here