Sulfurimonas autotrophica (strain ATCC BAA-671 / DSM 16294 / JCM 11897 / OK10)
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2155 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E0UPK6|E0UPK6_SULAO HI0933 family protein OS=Sulfurimonas autotrophica (strain ATCC BAA-671 / DSM 16294 / JCM 11897 / OK10) OX=563040 GN=Saut_0549 PE=4 SV=1
MM1 pKa = 7.8 LSTILEE7 pKa = 4.16 SLYY10 pKa = 11.15 LKK12 pKa = 10.61 VLVNIVVQRR21 pKa = 11.84 EE22 pKa = 4.16 STLVHH27 pKa = 6.84 IEE29 pKa = 3.97 LCSKK33 pKa = 10.48 KK34 pKa = 10.81 GIVDD38 pKa = 3.68 EE39 pKa = 4.86 VSTEE43 pKa = 4.1 FNTTLLNNQMYY54 pKa = 10.53 DD55 pKa = 4.12 FISEE59 pKa = 4.4 YY60 pKa = 10.25 IKK62 pKa = 10.32 EE63 pKa = 4.2 SPYY66 pKa = 10.49 FYY68 pKa = 10.54 ISILDD73 pKa = 3.56 TSIVQGAIPTCEE85 pKa = 4.03 KK86 pKa = 11.02 NKK88 pKa = 10.34 LSYY91 pKa = 11.06 YY92 pKa = 9.9 YY93 pKa = 9.84 DD94 pKa = 3.75 LSMSEE99 pKa = 4.43 YY100 pKa = 10.88 KK101 pKa = 11.01 CFDD104 pKa = 3.82 NKK106 pKa = 10.08 WNFYY110 pKa = 6.47 TAKK113 pKa = 10.58 NDD115 pKa = 2.63 IHH117 pKa = 7.64 QIEE120 pKa = 4.79 RR121 pKa = 11.84 EE122 pKa = 4.08 YY123 pKa = 11.24 EE124 pKa = 4.15 DD125 pKa = 4.07 IGIDD129 pKa = 3.51 MIFSPFVILANFFQDD144 pKa = 4.65 KK145 pKa = 10.46 INNNLAMYY153 pKa = 10.84 ALVQDD158 pKa = 4.29 GFISVAVFEE167 pKa = 4.5 NSQLLYY173 pKa = 10.91 AEE175 pKa = 4.84 HH176 pKa = 7.88 LDD178 pKa = 3.61 MLTGDD183 pKa = 3.94 EE184 pKa = 5.11 NEE186 pKa = 5.44 DD187 pKa = 3.15 ILLNEE192 pKa = 4.92 NIEE195 pKa = 4.1 EE196 pKa = 4.69 DD197 pKa = 3.87 LDD199 pKa = 4.34 LDD201 pKa = 4.54 SGIDD205 pKa = 3.98 LEE207 pKa = 6.32 DD208 pKa = 4.23 IDD210 pKa = 5.62 VDD212 pKa = 3.74 EE213 pKa = 5.59 GDD215 pKa = 4.05 INSLDD220 pKa = 3.63 EE221 pKa = 4.89 FGDD224 pKa = 3.92 IEE226 pKa = 5.35 DD227 pKa = 4.9 LDD229 pKa = 4.03 SLEE232 pKa = 5.79 DD233 pKa = 3.48 IDD235 pKa = 5.45 EE236 pKa = 4.67 FSDD239 pKa = 3.5 NKK241 pKa = 10.78 DD242 pKa = 3.43 VEE244 pKa = 4.45 EE245 pKa = 4.28 EE246 pKa = 4.46 LYY248 pKa = 10.86 EE249 pKa = 4.17 SDD251 pKa = 5.1 KK252 pKa = 11.08 ILPEE256 pKa = 4.32 DD257 pKa = 4.08 EE258 pKa = 4.09 EE259 pKa = 5.18 NEE261 pKa = 4.18 AFNEE265 pKa = 4.18 DD266 pKa = 3.7 YY267 pKa = 11.08 QRR269 pKa = 11.84 FSLMQSAVANFYY281 pKa = 10.8 RR282 pKa = 11.84 DD283 pKa = 3.3 DD284 pKa = 3.9 KK285 pKa = 11.05 YY286 pKa = 11.28 EE287 pKa = 3.91 SRR289 pKa = 11.84 FIEE292 pKa = 3.91 NVYY295 pKa = 10.35 IADD298 pKa = 4.0 CAGVTNDD305 pKa = 3.17 LKK307 pKa = 11.12 KK308 pKa = 10.8 YY309 pKa = 10.39 LEE311 pKa = 4.12 EE312 pKa = 3.91 EE313 pKa = 4.13 MFFNVYY319 pKa = 7.61 VRR321 pKa = 11.84 RR322 pKa = 11.84 ADD324 pKa = 3.69 LGVEE328 pKa = 4.11 VCEE331 pKa = 4.01 LAKK334 pKa = 10.23 MEE336 pKa = 5.07 LGLL339 pKa = 4.78
Molecular weight: 39.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.617
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.643
Grimsley 3.528
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.088
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.935
Patrickios 1.38
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.828
Protein with the highest isoelectric point:
>tr|E0UPP8|E0UPP8_SULAO Peptidase M23 OS=Sulfurimonas autotrophica (strain ATCC BAA-671 / DSM 16294 / JCM 11897 / OK10) OX=563040 GN=Saut_0591 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.32 RR14 pKa = 11.84 THH16 pKa = 6.0 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.32 NGRR28 pKa = 11.84 NIINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.99 GRR39 pKa = 11.84 KK40 pKa = 8.87 KK41 pKa = 10.21 LTVV44 pKa = 3.1
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2155
0
2155
672986
30
1662
312.3
35.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.079 ± 0.053
0.858 ± 0.021
5.685 ± 0.033
6.933 ± 0.063
4.868 ± 0.043
5.729 ± 0.056
2.039 ± 0.021
8.356 ± 0.057
8.715 ± 0.06
9.749 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.693 ± 0.023
5.311 ± 0.047
2.971 ± 0.034
3.195 ± 0.03
3.265 ± 0.038
6.261 ± 0.043
5.121 ± 0.046
6.357 ± 0.041
0.731 ± 0.018
4.084 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here