Vibrio phage phi 1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Pacinivirus; Vibrio virus phi1

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5HE27|A0A0B5HE27_9CAUD Uncharacterized protein OS=Vibrio phage phi 1 OX=1589297 GN=SBVP1_0092 PE=4 SV=1
MM1 pKa = 7.9DD2 pKa = 5.71LDD4 pKa = 5.05EE5 pKa = 6.37IITDD9 pKa = 3.89LSIGEE14 pKa = 4.19LQQHH18 pKa = 5.45GWFMGTLDD26 pKa = 3.77VNNKK30 pKa = 9.4RR31 pKa = 11.84KK32 pKa = 10.17LIACINIGLTEE43 pKa = 5.18LYY45 pKa = 10.27TRR47 pKa = 11.84FPLLTRR53 pKa = 11.84EE54 pKa = 3.94LTLIQVEE61 pKa = 4.42GRR63 pKa = 11.84TLYY66 pKa = 9.79PIKK69 pKa = 10.87VEE71 pKa = 3.93NTLVQGEE78 pKa = 4.31LPSYY82 pKa = 10.48NKK84 pKa = 10.8YY85 pKa = 9.84IDD87 pKa = 3.77YY88 pKa = 11.09VLDD91 pKa = 3.64YY92 pKa = 10.16PFTGDD97 pKa = 4.56LIRR100 pKa = 11.84VLSVYY105 pKa = 10.55DD106 pKa = 3.57EE107 pKa = 4.58EE108 pKa = 5.35GNEE111 pKa = 3.86IRR113 pKa = 11.84LNDD116 pKa = 3.43WTACPVVATPAMDD129 pKa = 3.56VLEE132 pKa = 4.51VPQPVVGDD140 pKa = 3.84ALFVIYY146 pKa = 9.17QAKK149 pKa = 9.39HH150 pKa = 5.16PKK152 pKa = 9.18IDD154 pKa = 3.62EE155 pKa = 4.65SNTTLYY161 pKa = 10.91LPLHH165 pKa = 6.59LKK167 pKa = 10.01PLLLAYY173 pKa = 9.64VAYY176 pKa = 9.8RR177 pKa = 11.84VYY179 pKa = 11.0SGGTTQEE186 pKa = 4.55HH187 pKa = 6.08IMLANTMLQKK197 pKa = 10.76FEE199 pKa = 4.83LLCTQQINLGTEE211 pKa = 3.48NSNDD215 pKa = 3.28YY216 pKa = 11.06DD217 pKa = 4.96RR218 pKa = 11.84NIKK221 pKa = 9.84PCMGGWVV228 pKa = 3.29

Molecular weight: 25.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5GYC5|A0A0B5GYC5_9CAUD Uncharacterized protein OS=Vibrio phage phi 1 OX=1589297 GN=SBVP1_0013 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.53LIPNQRR8 pKa = 11.84NVIVKK13 pKa = 8.69YY14 pKa = 10.26GRR16 pKa = 11.84TIRR19 pKa = 11.84LHH21 pKa = 6.75KK22 pKa = 10.48GFNPNEE28 pKa = 3.56YY29 pKa = 9.48RR30 pKa = 11.84HH31 pKa = 5.88WYY33 pKa = 9.29KK34 pKa = 10.72GKK36 pKa = 9.99QVVFYY41 pKa = 10.41YY42 pKa = 10.3EE43 pKa = 3.99INN45 pKa = 3.35

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

110

0

110

19985

37

3240

181.7

20.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.38 ± 0.428

0.906 ± 0.185

5.439 ± 0.159

7.135 ± 0.339

4.103 ± 0.187

5.354 ± 0.244

1.786 ± 0.222

6.64 ± 0.296

8.036 ± 0.261

9.167 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.587 ± 0.122

6.33 ± 0.19

3.698 ± 0.174

4.098 ± 0.217

3.923 ± 0.132

6.095 ± 0.213

6.57 ± 0.236

6.315 ± 0.34

1.186 ± 0.134

4.253 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski