Mesotoga sp. SC_4PWA21

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Kosmotogales; Kosmotogaceae; Mesotoga; unclassified Mesotoga

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A328INC4|A0A328INC4_9BACT HTH gntR-type domain-containing protein OS=Mesotoga sp. SC_4PWA21 OX=1487952 GN=DS67_04335 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.13KK3 pKa = 10.28KK4 pKa = 10.74SLIYY8 pKa = 10.34LLSLMVIATLFLFSACFRR26 pKa = 11.84LEE28 pKa = 3.56PSFTVEE34 pKa = 4.54LEE36 pKa = 4.21TTRR39 pKa = 11.84EE40 pKa = 4.2LIPVLNLSEE49 pKa = 4.31YY50 pKa = 10.61SLTVTVGEE58 pKa = 4.13GSYY61 pKa = 10.91VEE63 pKa = 5.25LSADD67 pKa = 3.3TGLVSEE73 pKa = 4.91MKK75 pKa = 10.52VAIAGGKK82 pKa = 10.07AEE84 pKa = 4.19IARR87 pKa = 11.84LFTEE91 pKa = 4.88SSAKK95 pKa = 10.12EE96 pKa = 3.66LTVSVKK102 pKa = 10.23NASGNEE108 pKa = 3.85IAKK111 pKa = 8.84KK112 pKa = 9.1TIALAGKK119 pKa = 8.56EE120 pKa = 4.18AEE122 pKa = 4.19FAIADD127 pKa = 4.11LATDD131 pKa = 5.07GIYY134 pKa = 10.13IPEE137 pKa = 4.24EE138 pKa = 3.85PAEE141 pKa = 4.24GEE143 pKa = 3.94PLEE146 pKa = 5.48FYY148 pKa = 11.18SLDD151 pKa = 4.01CIRR154 pKa = 11.84GKK156 pKa = 6.83TTPEE160 pKa = 4.02RR161 pKa = 11.84IVILLDD167 pKa = 3.36LEE169 pKa = 4.83DD170 pKa = 3.9RR171 pKa = 11.84GGYY174 pKa = 9.29ILLAGYY180 pKa = 9.47GWTWEE185 pKa = 4.65DD186 pKa = 3.64YY187 pKa = 10.86LVWLEE192 pKa = 4.64AGSPDD197 pKa = 3.84PEE199 pKa = 4.16EE200 pKa = 4.37AASLLPTHH208 pKa = 7.07RR209 pKa = 11.84AIDD212 pKa = 3.49KK213 pKa = 10.93CDD215 pKa = 3.34GDD217 pKa = 4.0KK218 pKa = 11.2LFNAVTGKK226 pKa = 10.5YY227 pKa = 9.78IYY229 pKa = 10.52VLTMSKK235 pKa = 10.35QDD237 pKa = 3.86LKK239 pKa = 11.16PGVGDD244 pKa = 4.81SGTTDD249 pKa = 3.61PALGAHH255 pKa = 6.35EE256 pKa = 4.57FDD258 pKa = 4.09EE259 pKa = 4.61GTIVDD264 pKa = 4.66LEE266 pKa = 4.24AFPGEE271 pKa = 3.47NWLFVEE277 pKa = 4.26WQGDD281 pKa = 4.11VVGSTVLIDD290 pKa = 3.47GDD292 pKa = 4.1KK293 pKa = 10.85EE294 pKa = 4.08VTAVFTPIEE303 pKa = 4.17HH304 pKa = 6.9LLEE307 pKa = 4.22IVNYY311 pKa = 9.06FPQGGNVDD319 pKa = 3.66VAAEE323 pKa = 4.12NVDD326 pKa = 4.48LDD328 pKa = 4.36YY329 pKa = 11.68LPMVPQGAEE338 pKa = 4.3LILTAVPSKK347 pKa = 10.3NWRR350 pKa = 11.84FVSWEE355 pKa = 3.96LPYY358 pKa = 11.13DD359 pKa = 3.72HH360 pKa = 6.63STKK363 pKa = 10.3NPEE366 pKa = 4.36YY367 pKa = 9.57FTMSAPATLKK377 pKa = 10.92VYY379 pKa = 8.27FAPIEE384 pKa = 4.19GKK386 pKa = 10.68YY387 pKa = 9.69PLTVGKK393 pKa = 10.3IGTGEE398 pKa = 4.36GNTTPVPGIYY408 pKa = 10.23NFNPGAEE415 pKa = 4.34VPLTASAASGSRR427 pKa = 11.84FDD429 pKa = 3.21RR430 pKa = 11.84WFVDD434 pKa = 3.43GLEE437 pKa = 4.09QAEE440 pKa = 4.47GAKK443 pKa = 10.41SIVVTIDD450 pKa = 2.89GTTTAMANFIKK461 pKa = 10.45LWTLNVDD468 pKa = 3.42KK469 pKa = 11.1VGEE472 pKa = 4.34GTTDD476 pKa = 3.65PEE478 pKa = 4.83PGSHH482 pKa = 6.5ILDD485 pKa = 3.54MGTKK489 pKa = 8.46KK490 pKa = 9.01TLTAKK495 pKa = 9.22ATAGWRR501 pKa = 11.84FDD503 pKa = 2.97RR504 pKa = 11.84WEE506 pKa = 3.86VDD508 pKa = 3.45GVPVEE513 pKa = 5.25DD514 pKa = 4.22EE515 pKa = 4.99DD516 pKa = 4.85GSITITVNADD526 pKa = 3.51TQATAYY532 pKa = 9.92FIKK535 pKa = 9.43QWKK538 pKa = 9.24VIVDD542 pKa = 3.62KK543 pKa = 11.47VGEE546 pKa = 4.32GTTAPEE552 pKa = 3.71SGIYY556 pKa = 10.08DD557 pKa = 4.47DD558 pKa = 4.68GTLLTFVATPADD570 pKa = 3.28GWRR573 pKa = 11.84FDD575 pKa = 2.94RR576 pKa = 11.84WVINGEE582 pKa = 3.92EE583 pKa = 4.17FLTEE587 pKa = 3.84EE588 pKa = 4.21VANYY592 pKa = 9.22PIVEE596 pKa = 4.32DD597 pKa = 4.09TLATAHH603 pKa = 7.17FIKK606 pKa = 10.49QYY608 pKa = 8.94TLTVNVIGEE617 pKa = 4.37GTTDD621 pKa = 3.67PEE623 pKa = 4.69EE624 pKa = 4.31GTHH627 pKa = 6.84IYY629 pKa = 10.72DD630 pKa = 3.17VDD632 pKa = 3.93TVVPLEE638 pKa = 4.04ASEE641 pKa = 4.18AFGWKK646 pKa = 8.89FDD648 pKa = 3.31KK649 pKa = 10.67WVVNGNEE656 pKa = 4.24TTDD659 pKa = 3.32NPTEE663 pKa = 3.94VLINADD669 pKa = 3.76SEE671 pKa = 4.32ATAVFTQLKK680 pKa = 9.12PEE682 pKa = 4.27VVVLDD687 pKa = 3.93APKK690 pKa = 10.05YY691 pKa = 9.52ACGTKK696 pKa = 10.19KK697 pKa = 9.43FTVLVSITYY706 pKa = 10.3ADD708 pKa = 3.5DD709 pKa = 3.59VKK711 pKa = 11.39FEE713 pKa = 4.45FEE715 pKa = 5.24DD716 pKa = 3.93GTTTIIDD723 pKa = 3.54VEE725 pKa = 4.64EE726 pKa = 4.23INGTKK731 pKa = 9.35IYY733 pKa = 9.59EE734 pKa = 4.39VEE736 pKa = 4.18VEE738 pKa = 4.25APSVPGPIPLIKK750 pKa = 10.44ALTVTASNDD759 pKa = 3.24AGEE762 pKa = 4.72TIVSKK767 pKa = 10.31PVSVLEE773 pKa = 4.83DD774 pKa = 3.2DD775 pKa = 3.93HH776 pKa = 6.73TPPEE780 pKa = 4.89IGDD783 pKa = 4.01FYY785 pKa = 11.93ADD787 pKa = 4.45LEE789 pKa = 4.73VGCDD793 pKa = 3.33ATGTTLYY800 pKa = 10.79FDD802 pKa = 5.04FFCDD806 pKa = 3.49PCKK809 pKa = 10.88DD810 pKa = 5.33DD811 pKa = 3.54VDD813 pKa = 4.16FWDD816 pKa = 3.99FEE818 pKa = 4.61KK819 pKa = 11.21NEE821 pKa = 4.4RR822 pKa = 11.84IEE824 pKa = 4.05VSHH827 pKa = 7.07GYY829 pKa = 9.66IDD831 pKa = 3.19WGKK834 pKa = 9.18PLDD837 pKa = 3.98AAPYY841 pKa = 9.94IEE843 pKa = 4.95GGRR846 pKa = 11.84CGYY849 pKa = 9.34SGKK852 pKa = 9.98VEE854 pKa = 4.16WVFEE858 pKa = 4.98DD859 pKa = 3.74IDD861 pKa = 4.62CEE863 pKa = 4.49TVMATVTIRR872 pKa = 11.84DD873 pKa = 3.45YY874 pKa = 11.39CGNEE878 pKa = 3.93NQSGLDD884 pKa = 3.71NGPLGPVDD892 pKa = 3.55NVYY895 pKa = 10.89PEE897 pKa = 5.39LISFKK902 pKa = 10.74QIGGFDD908 pKa = 3.83CDD910 pKa = 3.61DD911 pKa = 4.07PEE913 pKa = 6.22IIFEE917 pKa = 4.07FSIEE921 pKa = 4.19EE922 pKa = 3.9NCLCEE927 pKa = 4.44VEE929 pKa = 5.39LVSNCACDD937 pKa = 3.39WEE939 pKa = 4.82YY940 pKa = 11.39EE941 pKa = 4.14LSDD944 pKa = 3.81PATKK948 pKa = 10.25LGEE951 pKa = 4.03SGRR954 pKa = 11.84IVTTYY959 pKa = 9.23TATVTMLARR968 pKa = 11.84EE969 pKa = 4.4DD970 pKa = 3.59CTDD973 pKa = 3.34YY974 pKa = 11.45SFKK977 pKa = 11.11LLFADD982 pKa = 4.11CCGHH986 pKa = 6.91DD987 pKa = 4.93GEE989 pKa = 5.4SGWITVSKK997 pKa = 10.51DD998 pKa = 3.0LRR1000 pKa = 11.84APRR1003 pKa = 11.84IMMHH1007 pKa = 6.44GFLSSDD1013 pKa = 4.17LYY1015 pKa = 10.87TPSLNTIGDD1024 pKa = 3.76VVISVIGIINPITKK1038 pKa = 9.9EE1039 pKa = 3.8FLDD1042 pKa = 3.57IDD1044 pKa = 4.06LFEE1047 pKa = 5.65DD1048 pKa = 3.71VLPINLNDD1056 pKa = 3.54ILGSFQSVMDD1066 pKa = 5.07NIDD1069 pKa = 4.53LVPMDD1074 pKa = 3.65QASIINAEE1082 pKa = 4.11LEE1084 pKa = 4.19DD1085 pKa = 5.0LISMVEE1091 pKa = 4.31DD1092 pKa = 3.45YY1093 pKa = 11.59LMPCNADD1100 pKa = 2.89QGYY1103 pKa = 6.52YY1104 pKa = 8.37TWMVWDD1110 pKa = 4.61DD1111 pKa = 3.86CLKK1114 pKa = 9.58EE1115 pKa = 4.14TEE1117 pKa = 4.5ILYY1120 pKa = 10.63SDD1122 pKa = 4.73TICNTGKK1129 pKa = 10.46KK1130 pKa = 9.64VEE1132 pKa = 4.54EE1133 pKa = 4.21EE1134 pKa = 4.27TQPGYY1139 pKa = 10.75LVGYY1143 pKa = 9.25QRR1145 pKa = 11.84VEE1147 pKa = 3.8LCGLDD1152 pKa = 4.04CEE1154 pKa = 5.06TIQATLTAYY1163 pKa = 10.1DD1164 pKa = 3.37EE1165 pKa = 4.96CGNKK1169 pKa = 9.77SEE1171 pKa = 4.86ADD1173 pKa = 3.25ASKK1176 pKa = 10.97SIDD1179 pKa = 3.37NEE1181 pKa = 3.98APVIIDD1187 pKa = 4.62DD1188 pKa = 4.81FFVDD1192 pKa = 3.88YY1193 pKa = 10.92DD1194 pKa = 3.62KK1195 pKa = 11.22CTDD1198 pKa = 3.07IATISWDD1205 pKa = 3.39ATDD1208 pKa = 4.1GSGEE1212 pKa = 4.16VLVVIAADD1220 pKa = 3.69WGTMDD1225 pKa = 3.86QMSFGIVLDD1234 pKa = 3.72GTIIDD1239 pKa = 3.99VGATPTGQTVWNIPPINGEE1258 pKa = 4.11WVTVTMWVFDD1268 pKa = 3.74RR1269 pKa = 11.84CADD1272 pKa = 3.8CTGVCIDD1279 pKa = 5.03IEE1281 pKa = 4.49EE1282 pKa = 4.57GWLEE1286 pKa = 4.9DD1287 pKa = 4.24IEE1289 pKa = 4.73GCLGINMSKK1298 pKa = 7.86EE1299 pKa = 3.84TRR1301 pKa = 11.84EE1302 pKa = 3.84IFVDD1306 pKa = 3.57NMSPEE1311 pKa = 4.02ATFTLTGSCTDD1322 pKa = 3.29TEE1324 pKa = 4.46LLVEE1328 pKa = 3.93YY1329 pKa = 10.33GIYY1332 pKa = 10.18EE1333 pKa = 4.16GNLAEE1338 pKa = 4.93FEE1340 pKa = 4.5LLFEE1344 pKa = 4.62VFDD1347 pKa = 4.14SLEE1350 pKa = 4.07SSTVVASEE1358 pKa = 3.72VRR1360 pKa = 11.84TNKK1363 pKa = 9.4VDD1365 pKa = 3.66GQLLGAFEE1373 pKa = 4.18YY1374 pKa = 11.1LEE1376 pKa = 4.53YY1377 pKa = 11.05DD1378 pKa = 4.51DD1379 pKa = 5.86DD1380 pKa = 4.18TLMWTGSVTFDD1391 pKa = 4.56VDD1393 pKa = 4.64GLDD1396 pKa = 3.72CQLVRR1401 pKa = 11.84VTLTATDD1408 pKa = 3.0NCGNVDD1414 pKa = 3.97SVSKK1418 pKa = 9.51EE1419 pKa = 3.95TTIDD1423 pKa = 3.33NVVPEE1428 pKa = 4.6FEE1430 pKa = 4.05WSIVGVDD1437 pKa = 3.42EE1438 pKa = 5.13AGCITEE1444 pKa = 5.56DD1445 pKa = 3.96SFSLTWLATDD1455 pKa = 4.13GSNYY1459 pKa = 10.15YY1460 pKa = 10.44DD1461 pKa = 3.26ITIEE1465 pKa = 4.2ASWTGQVRR1473 pKa = 11.84RR1474 pKa = 11.84TRR1476 pKa = 11.84EE1477 pKa = 3.83SEE1479 pKa = 4.15EE1480 pKa = 3.62NSGAVEE1486 pKa = 4.18FNFDD1490 pKa = 4.19LDD1492 pKa = 4.1CVDD1495 pKa = 3.64VEE1497 pKa = 5.12FIFTIRR1503 pKa = 11.84DD1504 pKa = 3.37GCSDD1508 pKa = 3.71CGYY1511 pKa = 9.07TNVATEE1517 pKa = 4.29SKK1519 pKa = 10.47FYY1521 pKa = 10.48TRR1523 pKa = 11.84DD1524 pKa = 3.22SIAPEE1529 pKa = 4.01VEE1531 pKa = 3.57LDD1533 pKa = 4.99AEE1535 pKa = 4.41ISDD1538 pKa = 4.32CATEE1542 pKa = 5.39VVFDD1546 pKa = 3.89WSATDD1551 pKa = 3.25TCLDD1555 pKa = 3.53EE1556 pKa = 4.19VALYY1560 pKa = 10.61LRR1562 pKa = 11.84LEE1564 pKa = 4.5DD1565 pKa = 4.49CLTGEE1570 pKa = 4.34TTLLDD1575 pKa = 3.63TLVATGTYY1583 pKa = 10.33DD1584 pKa = 4.23FSLLTIEE1591 pKa = 4.44GTVTAILTATDD1602 pKa = 3.31TCGNSATDD1610 pKa = 3.56SVAFKK1615 pKa = 10.91VDD1617 pKa = 4.28TIAPRR1622 pKa = 11.84IDD1624 pKa = 2.82VDD1626 pKa = 3.41IYY1628 pKa = 11.03LDD1630 pKa = 3.84GVPVVDD1636 pKa = 6.27DD1637 pKa = 5.02EE1638 pKa = 4.76IGCFNASNTTITFVATATDD1657 pKa = 3.93CCLFGYY1663 pKa = 10.0DD1664 pKa = 3.39WEE1666 pKa = 4.4YY1667 pKa = 11.25LPEE1670 pKa = 4.75EE1671 pKa = 4.16IADD1674 pKa = 3.96CFTAVSTEE1682 pKa = 4.11LTVSATQFAIIVDD1695 pKa = 5.11FISNGATGTICPVTVTAAATDD1716 pKa = 3.79CCGNGSIWVTEE1727 pKa = 4.26FCFDD1731 pKa = 3.3NVLPGLEE1738 pKa = 3.81NFTFNSKK1745 pKa = 7.02TQIVKK1750 pKa = 9.73IDD1752 pKa = 3.63FNEE1755 pKa = 4.12DD1756 pKa = 3.27VKK1758 pKa = 11.63VGDD1761 pKa = 4.4DD1762 pKa = 3.37ATAALYY1768 pKa = 10.37VWLDD1772 pKa = 3.44PTNAPTGTDD1781 pKa = 3.16LSAPVFSDD1789 pKa = 3.4FNKK1792 pKa = 9.02WQLKK1796 pKa = 10.11ADD1798 pKa = 4.05YY1799 pKa = 9.44DD1800 pKa = 4.12SSAITPYY1807 pKa = 10.73SEE1809 pKa = 4.39TIKK1812 pKa = 11.13DD1813 pKa = 2.98SDD1815 pKa = 3.46IAVGVEE1821 pKa = 3.95SGTPYY1826 pKa = 10.55YY1827 pKa = 10.19QLQGGVWYY1835 pKa = 10.05GLSLEE1840 pKa = 4.92GIVDD1844 pKa = 3.54KK1845 pKa = 11.16CGNPAGEE1852 pKa = 4.07INIVGLAFDD1861 pKa = 4.75HH1862 pKa = 7.18APAPKK1867 pKa = 10.02FF1868 pKa = 3.43

Molecular weight:
204.27 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A328INZ0|A0A328INZ0_9BACT Uncharacterized protein OS=Mesotoga sp. SC_4PWA21 OX=1487952 GN=DS67_02395 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.16QPSRR9 pKa = 11.84VKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 6.49GFLVRR21 pKa = 11.84SRR23 pKa = 11.84TVGGRR28 pKa = 11.84KK29 pKa = 9.06VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.61GRR39 pKa = 11.84KK40 pKa = 8.77RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight:
5.24 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1273

0

1273

375127

41

2008

294.7

33.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.696 ± 0.064

0.849 ± 0.026

5.43 ± 0.058

7.657 ± 0.072

4.971 ± 0.059

7.276 ± 0.054

1.526 ± 0.029

7.547 ± 0.047

5.746 ± 0.069

10.086 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.611 ± 0.031

3.785 ± 0.037

3.741 ± 0.035

2.279 ± 0.031

5.476 ± 0.058

7.433 ± 0.059

4.926 ± 0.052

7.476 ± 0.049

1.063 ± 0.028

3.427 ± 0.046

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski