Mesotoga sp. SC_4PWA21
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1273 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A328INC4|A0A328INC4_9BACT HTH gntR-type domain-containing protein OS=Mesotoga sp. SC_4PWA21 OX=1487952 GN=DS67_04335 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.13 KK3 pKa = 10.28 KK4 pKa = 10.74 SLIYY8 pKa = 10.34 LLSLMVIATLFLFSACFRR26 pKa = 11.84 LEE28 pKa = 3.56 PSFTVEE34 pKa = 4.54 LEE36 pKa = 4.21 TTRR39 pKa = 11.84 EE40 pKa = 4.2 LIPVLNLSEE49 pKa = 4.31 YY50 pKa = 10.61 SLTVTVGEE58 pKa = 4.13 GSYY61 pKa = 10.91 VEE63 pKa = 5.25 LSADD67 pKa = 3.3 TGLVSEE73 pKa = 4.91 MKK75 pKa = 10.52 VAIAGGKK82 pKa = 10.07 AEE84 pKa = 4.19 IARR87 pKa = 11.84 LFTEE91 pKa = 4.88 SSAKK95 pKa = 10.12 EE96 pKa = 3.66 LTVSVKK102 pKa = 10.23 NASGNEE108 pKa = 3.85 IAKK111 pKa = 8.84 KK112 pKa = 9.1 TIALAGKK119 pKa = 8.56 EE120 pKa = 4.18 AEE122 pKa = 4.19 FAIADD127 pKa = 4.11 LATDD131 pKa = 5.07 GIYY134 pKa = 10.13 IPEE137 pKa = 4.24 EE138 pKa = 3.85 PAEE141 pKa = 4.24 GEE143 pKa = 3.94 PLEE146 pKa = 5.48 FYY148 pKa = 11.18 SLDD151 pKa = 4.01 CIRR154 pKa = 11.84 GKK156 pKa = 6.83 TTPEE160 pKa = 4.02 RR161 pKa = 11.84 IVILLDD167 pKa = 3.36 LEE169 pKa = 4.83 DD170 pKa = 3.9 RR171 pKa = 11.84 GGYY174 pKa = 9.29 ILLAGYY180 pKa = 9.47 GWTWEE185 pKa = 4.65 DD186 pKa = 3.64 YY187 pKa = 10.86 LVWLEE192 pKa = 4.64 AGSPDD197 pKa = 3.84 PEE199 pKa = 4.16 EE200 pKa = 4.37 AASLLPTHH208 pKa = 7.07 RR209 pKa = 11.84 AIDD212 pKa = 3.49 KK213 pKa = 10.93 CDD215 pKa = 3.34 GDD217 pKa = 4.0 KK218 pKa = 11.2 LFNAVTGKK226 pKa = 10.5 YY227 pKa = 9.78 IYY229 pKa = 10.52 VLTMSKK235 pKa = 10.35 QDD237 pKa = 3.86 LKK239 pKa = 11.16 PGVGDD244 pKa = 4.81 SGTTDD249 pKa = 3.61 PALGAHH255 pKa = 6.35 EE256 pKa = 4.57 FDD258 pKa = 4.09 EE259 pKa = 4.61 GTIVDD264 pKa = 4.66 LEE266 pKa = 4.24 AFPGEE271 pKa = 3.47 NWLFVEE277 pKa = 4.26 WQGDD281 pKa = 4.11 VVGSTVLIDD290 pKa = 3.47 GDD292 pKa = 4.1 KK293 pKa = 10.85 EE294 pKa = 4.08 VTAVFTPIEE303 pKa = 4.17 HH304 pKa = 6.9 LLEE307 pKa = 4.22 IVNYY311 pKa = 9.06 FPQGGNVDD319 pKa = 3.66 VAAEE323 pKa = 4.12 NVDD326 pKa = 4.48 LDD328 pKa = 4.36 YY329 pKa = 11.68 LPMVPQGAEE338 pKa = 4.3 LILTAVPSKK347 pKa = 10.3 NWRR350 pKa = 11.84 FVSWEE355 pKa = 3.96 LPYY358 pKa = 11.13 DD359 pKa = 3.72 HH360 pKa = 6.63 STKK363 pKa = 10.3 NPEE366 pKa = 4.36 YY367 pKa = 9.57 FTMSAPATLKK377 pKa = 10.92 VYY379 pKa = 8.27 FAPIEE384 pKa = 4.19 GKK386 pKa = 10.68 YY387 pKa = 9.69 PLTVGKK393 pKa = 10.3 IGTGEE398 pKa = 4.36 GNTTPVPGIYY408 pKa = 10.23 NFNPGAEE415 pKa = 4.34 VPLTASAASGSRR427 pKa = 11.84 FDD429 pKa = 3.21 RR430 pKa = 11.84 WFVDD434 pKa = 3.43 GLEE437 pKa = 4.09 QAEE440 pKa = 4.47 GAKK443 pKa = 10.41 SIVVTIDD450 pKa = 2.89 GTTTAMANFIKK461 pKa = 10.45 LWTLNVDD468 pKa = 3.42 KK469 pKa = 11.1 VGEE472 pKa = 4.34 GTTDD476 pKa = 3.65 PEE478 pKa = 4.83 PGSHH482 pKa = 6.5 ILDD485 pKa = 3.54 MGTKK489 pKa = 8.46 KK490 pKa = 9.01 TLTAKK495 pKa = 9.22 ATAGWRR501 pKa = 11.84 FDD503 pKa = 2.97 RR504 pKa = 11.84 WEE506 pKa = 3.86 VDD508 pKa = 3.45 GVPVEE513 pKa = 5.25 DD514 pKa = 4.22 EE515 pKa = 4.99 DD516 pKa = 4.85 GSITITVNADD526 pKa = 3.51 TQATAYY532 pKa = 9.92 FIKK535 pKa = 9.43 QWKK538 pKa = 9.24 VIVDD542 pKa = 3.62 KK543 pKa = 11.47 VGEE546 pKa = 4.32 GTTAPEE552 pKa = 3.71 SGIYY556 pKa = 10.08 DD557 pKa = 4.47 DD558 pKa = 4.68 GTLLTFVATPADD570 pKa = 3.28 GWRR573 pKa = 11.84 FDD575 pKa = 2.94 RR576 pKa = 11.84 WVINGEE582 pKa = 3.92 EE583 pKa = 4.17 FLTEE587 pKa = 3.84 EE588 pKa = 4.21 VANYY592 pKa = 9.22 PIVEE596 pKa = 4.32 DD597 pKa = 4.09 TLATAHH603 pKa = 7.17 FIKK606 pKa = 10.49 QYY608 pKa = 8.94 TLTVNVIGEE617 pKa = 4.37 GTTDD621 pKa = 3.67 PEE623 pKa = 4.69 EE624 pKa = 4.31 GTHH627 pKa = 6.84 IYY629 pKa = 10.72 DD630 pKa = 3.17 VDD632 pKa = 3.93 TVVPLEE638 pKa = 4.04 ASEE641 pKa = 4.18 AFGWKK646 pKa = 8.89 FDD648 pKa = 3.31 KK649 pKa = 10.67 WVVNGNEE656 pKa = 4.24 TTDD659 pKa = 3.32 NPTEE663 pKa = 3.94 VLINADD669 pKa = 3.76 SEE671 pKa = 4.32 ATAVFTQLKK680 pKa = 9.12 PEE682 pKa = 4.27 VVVLDD687 pKa = 3.93 APKK690 pKa = 10.05 YY691 pKa = 9.52 ACGTKK696 pKa = 10.19 KK697 pKa = 9.43 FTVLVSITYY706 pKa = 10.3 ADD708 pKa = 3.5 DD709 pKa = 3.59 VKK711 pKa = 11.39 FEE713 pKa = 4.45 FEE715 pKa = 5.24 DD716 pKa = 3.93 GTTTIIDD723 pKa = 3.54 VEE725 pKa = 4.64 EE726 pKa = 4.23 INGTKK731 pKa = 9.35 IYY733 pKa = 9.59 EE734 pKa = 4.39 VEE736 pKa = 4.18 VEE738 pKa = 4.25 APSVPGPIPLIKK750 pKa = 10.44 ALTVTASNDD759 pKa = 3.24 AGEE762 pKa = 4.72 TIVSKK767 pKa = 10.31 PVSVLEE773 pKa = 4.83 DD774 pKa = 3.2 DD775 pKa = 3.93 HH776 pKa = 6.73 TPPEE780 pKa = 4.89 IGDD783 pKa = 4.01 FYY785 pKa = 11.93 ADD787 pKa = 4.45 LEE789 pKa = 4.73 VGCDD793 pKa = 3.33 ATGTTLYY800 pKa = 10.79 FDD802 pKa = 5.04 FFCDD806 pKa = 3.49 PCKK809 pKa = 10.88 DD810 pKa = 5.33 DD811 pKa = 3.54 VDD813 pKa = 4.16 FWDD816 pKa = 3.99 FEE818 pKa = 4.61 KK819 pKa = 11.21 NEE821 pKa = 4.4 RR822 pKa = 11.84 IEE824 pKa = 4.05 VSHH827 pKa = 7.07 GYY829 pKa = 9.66 IDD831 pKa = 3.19 WGKK834 pKa = 9.18 PLDD837 pKa = 3.98 AAPYY841 pKa = 9.94 IEE843 pKa = 4.95 GGRR846 pKa = 11.84 CGYY849 pKa = 9.34 SGKK852 pKa = 9.98 VEE854 pKa = 4.16 WVFEE858 pKa = 4.98 DD859 pKa = 3.74 IDD861 pKa = 4.62 CEE863 pKa = 4.49 TVMATVTIRR872 pKa = 11.84 DD873 pKa = 3.45 YY874 pKa = 11.39 CGNEE878 pKa = 3.93 NQSGLDD884 pKa = 3.71 NGPLGPVDD892 pKa = 3.55 NVYY895 pKa = 10.89 PEE897 pKa = 5.39 LISFKK902 pKa = 10.74 QIGGFDD908 pKa = 3.83 CDD910 pKa = 3.61 DD911 pKa = 4.07 PEE913 pKa = 6.22 IIFEE917 pKa = 4.07 FSIEE921 pKa = 4.19 EE922 pKa = 3.9 NCLCEE927 pKa = 4.44 VEE929 pKa = 5.39 LVSNCACDD937 pKa = 3.39 WEE939 pKa = 4.82 YY940 pKa = 11.39 EE941 pKa = 4.14 LSDD944 pKa = 3.81 PATKK948 pKa = 10.25 LGEE951 pKa = 4.03 SGRR954 pKa = 11.84 IVTTYY959 pKa = 9.23 TATVTMLARR968 pKa = 11.84 EE969 pKa = 4.4 DD970 pKa = 3.59 CTDD973 pKa = 3.34 YY974 pKa = 11.45 SFKK977 pKa = 11.11 LLFADD982 pKa = 4.11 CCGHH986 pKa = 6.91 DD987 pKa = 4.93 GEE989 pKa = 5.4 SGWITVSKK997 pKa = 10.51 DD998 pKa = 3.0 LRR1000 pKa = 11.84 APRR1003 pKa = 11.84 IMMHH1007 pKa = 6.44 GFLSSDD1013 pKa = 4.17 LYY1015 pKa = 10.87 TPSLNTIGDD1024 pKa = 3.76 VVISVIGIINPITKK1038 pKa = 9.9 EE1039 pKa = 3.8 FLDD1042 pKa = 3.57 IDD1044 pKa = 4.06 LFEE1047 pKa = 5.65 DD1048 pKa = 3.71 VLPINLNDD1056 pKa = 3.54 ILGSFQSVMDD1066 pKa = 5.07 NIDD1069 pKa = 4.53 LVPMDD1074 pKa = 3.65 QASIINAEE1082 pKa = 4.11 LEE1084 pKa = 4.19 DD1085 pKa = 5.0 LISMVEE1091 pKa = 4.31 DD1092 pKa = 3.45 YY1093 pKa = 11.59 LMPCNADD1100 pKa = 2.89 QGYY1103 pKa = 6.52 YY1104 pKa = 8.37 TWMVWDD1110 pKa = 4.61 DD1111 pKa = 3.86 CLKK1114 pKa = 9.58 EE1115 pKa = 4.14 TEE1117 pKa = 4.5 ILYY1120 pKa = 10.63 SDD1122 pKa = 4.73 TICNTGKK1129 pKa = 10.46 KK1130 pKa = 9.64 VEE1132 pKa = 4.54 EE1133 pKa = 4.21 EE1134 pKa = 4.27 TQPGYY1139 pKa = 10.75 LVGYY1143 pKa = 9.25 QRR1145 pKa = 11.84 VEE1147 pKa = 3.8 LCGLDD1152 pKa = 4.04 CEE1154 pKa = 5.06 TIQATLTAYY1163 pKa = 10.1 DD1164 pKa = 3.37 EE1165 pKa = 4.96 CGNKK1169 pKa = 9.77 SEE1171 pKa = 4.86 ADD1173 pKa = 3.25 ASKK1176 pKa = 10.97 SIDD1179 pKa = 3.37 NEE1181 pKa = 3.98 APVIIDD1187 pKa = 4.62 DD1188 pKa = 4.81 FFVDD1192 pKa = 3.88 YY1193 pKa = 10.92 DD1194 pKa = 3.62 KK1195 pKa = 11.22 CTDD1198 pKa = 3.07 IATISWDD1205 pKa = 3.39 ATDD1208 pKa = 4.1 GSGEE1212 pKa = 4.16 VLVVIAADD1220 pKa = 3.69 WGTMDD1225 pKa = 3.86 QMSFGIVLDD1234 pKa = 3.72 GTIIDD1239 pKa = 3.99 VGATPTGQTVWNIPPINGEE1258 pKa = 4.11 WVTVTMWVFDD1268 pKa = 3.74 RR1269 pKa = 11.84 CADD1272 pKa = 3.8 CTGVCIDD1279 pKa = 5.03 IEE1281 pKa = 4.49 EE1282 pKa = 4.57 GWLEE1286 pKa = 4.9 DD1287 pKa = 4.24 IEE1289 pKa = 4.73 GCLGINMSKK1298 pKa = 7.86 EE1299 pKa = 3.84 TRR1301 pKa = 11.84 EE1302 pKa = 3.84 IFVDD1306 pKa = 3.57 NMSPEE1311 pKa = 4.02 ATFTLTGSCTDD1322 pKa = 3.29 TEE1324 pKa = 4.46 LLVEE1328 pKa = 3.93 YY1329 pKa = 10.33 GIYY1332 pKa = 10.18 EE1333 pKa = 4.16 GNLAEE1338 pKa = 4.93 FEE1340 pKa = 4.5 LLFEE1344 pKa = 4.62 VFDD1347 pKa = 4.14 SLEE1350 pKa = 4.07 SSTVVASEE1358 pKa = 3.72 VRR1360 pKa = 11.84 TNKK1363 pKa = 9.4 VDD1365 pKa = 3.66 GQLLGAFEE1373 pKa = 4.18 YY1374 pKa = 11.1 LEE1376 pKa = 4.53 YY1377 pKa = 11.05 DD1378 pKa = 4.51 DD1379 pKa = 5.86 DD1380 pKa = 4.18 TLMWTGSVTFDD1391 pKa = 4.56 VDD1393 pKa = 4.64 GLDD1396 pKa = 3.72 CQLVRR1401 pKa = 11.84 VTLTATDD1408 pKa = 3.0 NCGNVDD1414 pKa = 3.97 SVSKK1418 pKa = 9.51 EE1419 pKa = 3.95 TTIDD1423 pKa = 3.33 NVVPEE1428 pKa = 4.6 FEE1430 pKa = 4.05 WSIVGVDD1437 pKa = 3.42 EE1438 pKa = 5.13 AGCITEE1444 pKa = 5.56 DD1445 pKa = 3.96 SFSLTWLATDD1455 pKa = 4.13 GSNYY1459 pKa = 10.15 YY1460 pKa = 10.44 DD1461 pKa = 3.26 ITIEE1465 pKa = 4.2 ASWTGQVRR1473 pKa = 11.84 RR1474 pKa = 11.84 TRR1476 pKa = 11.84 EE1477 pKa = 3.83 SEE1479 pKa = 4.15 EE1480 pKa = 3.62 NSGAVEE1486 pKa = 4.18 FNFDD1490 pKa = 4.19 LDD1492 pKa = 4.1 CVDD1495 pKa = 3.64 VEE1497 pKa = 5.12 FIFTIRR1503 pKa = 11.84 DD1504 pKa = 3.37 GCSDD1508 pKa = 3.71 CGYY1511 pKa = 9.07 TNVATEE1517 pKa = 4.29 SKK1519 pKa = 10.47 FYY1521 pKa = 10.48 TRR1523 pKa = 11.84 DD1524 pKa = 3.22 SIAPEE1529 pKa = 4.01 VEE1531 pKa = 3.57 LDD1533 pKa = 4.99 AEE1535 pKa = 4.41 ISDD1538 pKa = 4.32 CATEE1542 pKa = 5.39 VVFDD1546 pKa = 3.89 WSATDD1551 pKa = 3.25 TCLDD1555 pKa = 3.53 EE1556 pKa = 4.19 VALYY1560 pKa = 10.61 LRR1562 pKa = 11.84 LEE1564 pKa = 4.5 DD1565 pKa = 4.49 CLTGEE1570 pKa = 4.34 TTLLDD1575 pKa = 3.63 TLVATGTYY1583 pKa = 10.33 DD1584 pKa = 4.23 FSLLTIEE1591 pKa = 4.44 GTVTAILTATDD1602 pKa = 3.31 TCGNSATDD1610 pKa = 3.56 SVAFKK1615 pKa = 10.91 VDD1617 pKa = 4.28 TIAPRR1622 pKa = 11.84 IDD1624 pKa = 2.82 VDD1626 pKa = 3.41 IYY1628 pKa = 11.03 LDD1630 pKa = 3.84 GVPVVDD1636 pKa = 6.27 DD1637 pKa = 5.02 EE1638 pKa = 4.76 IGCFNASNTTITFVATATDD1657 pKa = 3.93 CCLFGYY1663 pKa = 10.0 DD1664 pKa = 3.39 WEE1666 pKa = 4.4 YY1667 pKa = 11.25 LPEE1670 pKa = 4.75 EE1671 pKa = 4.16 IADD1674 pKa = 3.96 CFTAVSTEE1682 pKa = 4.11 LTVSATQFAIIVDD1695 pKa = 5.11 FISNGATGTICPVTVTAAATDD1716 pKa = 3.79 CCGNGSIWVTEE1727 pKa = 4.26 FCFDD1731 pKa = 3.3 NVLPGLEE1738 pKa = 3.81 NFTFNSKK1745 pKa = 7.02 TQIVKK1750 pKa = 9.73 IDD1752 pKa = 3.63 FNEE1755 pKa = 4.12 DD1756 pKa = 3.27 VKK1758 pKa = 11.63 VGDD1761 pKa = 4.4 DD1762 pKa = 3.37 ATAALYY1768 pKa = 10.37 VWLDD1772 pKa = 3.44 PTNAPTGTDD1781 pKa = 3.16 LSAPVFSDD1789 pKa = 3.4 FNKK1792 pKa = 9.02 WQLKK1796 pKa = 10.11 ADD1798 pKa = 4.05 YY1799 pKa = 9.44 DD1800 pKa = 4.12 SSAITPYY1807 pKa = 10.73 SEE1809 pKa = 4.39 TIKK1812 pKa = 11.13 DD1813 pKa = 2.98 SDD1815 pKa = 3.46 IAVGVEE1821 pKa = 3.95 SGTPYY1826 pKa = 10.55 YY1827 pKa = 10.19 QLQGGVWYY1835 pKa = 10.05 GLSLEE1840 pKa = 4.92 GIVDD1844 pKa = 3.54 KK1845 pKa = 11.16 CGNPAGEE1852 pKa = 4.07 INIVGLAFDD1861 pKa = 4.75 HH1862 pKa = 7.18 APAPKK1867 pKa = 10.02 FF1868 pKa = 3.43
Molecular weight: 204.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.795
IPC_protein 3.834
Toseland 3.617
ProMoST 3.961
Dawson 3.808
Bjellqvist 3.948
Wikipedia 3.706
Rodwell 3.656
Grimsley 3.516
Solomon 3.808
Lehninger 3.757
Nozaki 3.91
DTASelect 4.126
Thurlkill 3.643
EMBOSS 3.719
Sillero 3.948
Patrickios 1.151
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.834
Protein with the highest isoelectric point:
>tr|A0A328INZ0|A0A328INZ0_9BACT Uncharacterized protein OS=Mesotoga sp. SC_4PWA21 OX=1487952 GN=DS67_02395 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.16 QPSRR9 pKa = 11.84 VKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 THH16 pKa = 6.49 GFLVRR21 pKa = 11.84 SRR23 pKa = 11.84 TVGGRR28 pKa = 11.84 KK29 pKa = 9.06 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.61 GRR39 pKa = 11.84 KK40 pKa = 8.77 RR41 pKa = 11.84 LSVV44 pKa = 3.2
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1273
0
1273
375127
41
2008
294.7
33.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.696 ± 0.064
0.849 ± 0.026
5.43 ± 0.058
7.657 ± 0.072
4.971 ± 0.059
7.276 ± 0.054
1.526 ± 0.029
7.547 ± 0.047
5.746 ± 0.069
10.086 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.611 ± 0.031
3.785 ± 0.037
3.741 ± 0.035
2.279 ± 0.031
5.476 ± 0.058
7.433 ± 0.059
4.926 ± 0.052
7.476 ± 0.049
1.063 ± 0.028
3.427 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here