Amino acid dipepetide frequency for Mesotoga sp. SC_4PWA21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.406AlaAla: 5.406 ± 0.163
0.6AlaCys: 0.6 ± 0.039
3.207AlaAsp: 3.207 ± 0.1
4.353AlaGlu: 4.353 ± 0.118
3.466AlaPhe: 3.466 ± 0.105
5.308AlaGly: 5.308 ± 0.122
0.973AlaHis: 0.973 ± 0.051
5.502AlaIle: 5.502 ± 0.147
3.505AlaLys: 3.505 ± 0.107
7.283AlaLeu: 7.283 ± 0.162
1.957AlaMet: 1.957 ± 0.068
2.261AlaAsn: 2.261 ± 0.077
1.922AlaPro: 1.922 ± 0.071
1.469AlaGln: 1.469 ± 0.06
3.673AlaArg: 3.673 ± 0.107
4.75AlaSer: 4.75 ± 0.109
3.036AlaThr: 3.036 ± 0.1
5.571AlaVal: 5.571 ± 0.153
0.621AlaTrp: 0.621 ± 0.04
2.098AlaTyr: 2.098 ± 0.077
0.0AlaXaa: 0.0 ± 0.0
Cys
0.456CysAla: 0.456 ± 0.04
0.091CysCys: 0.091 ± 0.016
0.491CysAsp: 0.491 ± 0.04
0.597CysGlu: 0.597 ± 0.042
0.429CysPhe: 0.429 ± 0.035
0.845CysGly: 0.845 ± 0.054
0.179CysHis: 0.179 ± 0.023
0.544CysIle: 0.544 ± 0.041
0.453CysLys: 0.453 ± 0.039
0.722CysLeu: 0.722 ± 0.043
0.165CysMet: 0.165 ± 0.019
0.328CysAsn: 0.328 ± 0.03
0.475CysPro: 0.475 ± 0.039
0.203CysGln: 0.203 ± 0.023
0.501CysArg: 0.501 ± 0.04
0.685CysSer: 0.685 ± 0.041
0.464CysThr: 0.464 ± 0.038
0.491CysVal: 0.491 ± 0.039
0.117CysTrp: 0.117 ± 0.021
0.259CysTyr: 0.259 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
3.202AspAla: 3.202 ± 0.094
0.544AspCys: 0.544 ± 0.06
2.663AspAsp: 2.663 ± 0.09
4.121AspGlu: 4.121 ± 0.118
3.132AspPhe: 3.132 ± 0.095
3.799AspGly: 3.799 ± 0.11
0.912AspHis: 0.912 ± 0.049
3.857AspIle: 3.857 ± 0.106
2.58AspLys: 2.58 ± 0.078
6.083AspLeu: 6.083 ± 0.14
1.216AspMet: 1.216 ± 0.057
2.021AspAsn: 2.021 ± 0.072
2.674AspPro: 2.674 ± 0.094
1.256AspGln: 1.256 ± 0.061
3.172AspArg: 3.172 ± 0.108
3.951AspSer: 3.951 ± 0.116
2.402AspThr: 2.402 ± 0.078
3.679AspVal: 3.679 ± 0.103
0.661AspTrp: 0.661 ± 0.045
2.373AspTyr: 2.373 ± 0.084
0.0AspXaa: 0.0 ± 0.0
Glu
5.196GluAla: 5.196 ± 0.132
0.488GluCys: 0.488 ± 0.035
3.879GluAsp: 3.879 ± 0.1
6.939GluGlu: 6.939 ± 0.189
3.319GluPhe: 3.319 ± 0.08
4.897GluGly: 4.897 ± 0.105
1.013GluHis: 1.013 ± 0.058
6.72GluIle: 6.72 ± 0.139
5.382GluLys: 5.382 ± 0.131
7.493GluLeu: 7.493 ± 0.177
2.322GluMet: 2.322 ± 0.089
3.22GluAsn: 3.22 ± 0.102
2.13GluPro: 2.13 ± 0.088
1.589GluGln: 1.589 ± 0.063
4.849GluArg: 4.849 ± 0.12
4.732GluSer: 4.732 ± 0.127
3.684GluThr: 3.684 ± 0.107
5.446GluVal: 5.446 ± 0.118
0.744GluTrp: 0.744 ± 0.045
2.532GluTyr: 2.532 ± 0.095
0.0GluXaa: 0.0 ± 0.0
Phe
3.29PheAla: 3.29 ± 0.092
0.496PheCys: 0.496 ± 0.04
3.183PheAsp: 3.183 ± 0.091
3.772PheGlu: 3.772 ± 0.093
2.655PhePhe: 2.655 ± 0.094
4.065PheGly: 4.065 ± 0.103
0.72PheHis: 0.72 ± 0.046
3.151PheIle: 3.151 ± 0.101
2.173PheLys: 2.173 ± 0.066
4.937PheLeu: 4.937 ± 0.139
1.28PheMet: 1.28 ± 0.062
1.711PheAsn: 1.711 ± 0.08
1.938PhePro: 1.938 ± 0.062
1.122PheGln: 1.122 ± 0.056
2.492PheArg: 2.492 ± 0.076
4.311PheSer: 4.311 ± 0.098
2.41PheThr: 2.41 ± 0.094
3.737PheVal: 3.737 ± 0.113
0.618PheTrp: 0.618 ± 0.043
1.653PheTyr: 1.653 ± 0.074
0.0PheXaa: 0.0 ± 0.0
Gly
4.585GlyAla: 4.585 ± 0.116
0.68GlyCys: 0.68 ± 0.044
3.583GlyAsp: 3.583 ± 0.091
5.166GlyGlu: 5.166 ± 0.151
3.801GlyPhe: 3.801 ± 0.095
4.942GlyGly: 4.942 ± 0.138
1.194GlyHis: 1.194 ± 0.056
6.137GlyIle: 6.137 ± 0.142
4.9GlyLys: 4.9 ± 0.124
6.896GlyLeu: 6.896 ± 0.142
2.143GlyMet: 2.143 ± 0.075
2.826GlyAsn: 2.826 ± 0.098
1.994GlyPro: 1.994 ± 0.079
1.69GlyGln: 1.69 ± 0.07
3.964GlyArg: 3.964 ± 0.108
5.1GlySer: 5.1 ± 0.122
4.124GlyThr: 4.124 ± 0.129
5.286GlyVal: 5.286 ± 0.12
0.92GlyTrp: 0.92 ± 0.061
2.828GlyTyr: 2.828 ± 0.086
0.0GlyXaa: 0.0 ± 0.0
His
0.936HisAla: 0.936 ± 0.052
0.171HisCys: 0.171 ± 0.02
0.813HisAsp: 0.813 ± 0.049
1.026HisGlu: 1.026 ± 0.051
0.834HisPhe: 0.834 ± 0.049
1.277HisGly: 1.277 ± 0.06
0.339HisHis: 0.339 ± 0.036
1.09HisIle: 1.09 ± 0.048
0.685HisLys: 0.685 ± 0.042
1.509HisLeu: 1.509 ± 0.065
0.363HisMet: 0.363 ± 0.033
0.658HisAsn: 0.658 ± 0.042
0.794HisPro: 0.794 ± 0.051
0.317HisGln: 0.317 ± 0.031
0.776HisArg: 0.776 ± 0.047
1.154HisSer: 1.154 ± 0.056
0.706HisThr: 0.706 ± 0.042
1.005HisVal: 1.005 ± 0.061
0.163HisTrp: 0.163 ± 0.019
0.645HisTyr: 0.645 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
5.745IleAla: 5.745 ± 0.138
0.698IleCys: 0.698 ± 0.047
4.945IleAsp: 4.945 ± 0.112
6.142IleGlu: 6.142 ± 0.155
3.378IlePhe: 3.378 ± 0.099
5.515IleGly: 5.515 ± 0.132
1.146IleHis: 1.146 ± 0.059
4.98IleIle: 4.98 ± 0.153
3.543IleLys: 3.543 ± 0.105
6.966IleLeu: 6.966 ± 0.138
1.901IleMet: 1.901 ± 0.074
2.431IleAsn: 2.431 ± 0.082
3.466IlePro: 3.466 ± 0.093
1.629IleGln: 1.629 ± 0.059
3.703IleArg: 3.703 ± 0.099
6.073IleSer: 6.073 ± 0.138
3.935IleThr: 3.935 ± 0.117
6.302IleVal: 6.302 ± 0.132
0.738IleTrp: 0.738 ± 0.046
2.234IleTyr: 2.234 ± 0.082
0.0IleXaa: 0.0 ± 0.0
Lys
4.111LysAla: 4.111 ± 0.125
0.421LysCys: 0.421 ± 0.04
2.988LysAsp: 2.988 ± 0.101
4.94LysGlu: 4.94 ± 0.14
1.871LysPhe: 1.871 ± 0.075
3.972LysGly: 3.972 ± 0.105
0.936LysHis: 0.936 ± 0.054
4.225LysIle: 4.225 ± 0.105
4.087LysLys: 4.087 ± 0.128
5.249LysLeu: 5.249 ± 0.113
1.674LysMet: 1.674 ± 0.065
2.554LysAsn: 2.554 ± 0.102
1.842LysPro: 1.842 ± 0.069
1.266LysGln: 1.266 ± 0.064
3.543LysArg: 3.543 ± 0.113
4.031LysSer: 4.031 ± 0.119
3.332LysThr: 3.332 ± 0.097
3.911LysVal: 3.911 ± 0.122
0.491LysTrp: 0.491 ± 0.034
2.013LysTyr: 2.013 ± 0.072
0.0LysXaa: 0.0 ± 0.0
Leu
6.728LeuAla: 6.728 ± 0.153
0.768LeuCys: 0.768 ± 0.047
5.425LeuAsp: 5.425 ± 0.125
7.589LeuGlu: 7.589 ± 0.186
5.193LeuPhe: 5.193 ± 0.148
6.952LeuGly: 6.952 ± 0.135
1.36LeuHis: 1.36 ± 0.061
6.928LeuIle: 6.928 ± 0.154
6.728LeuLys: 6.728 ± 0.159
10.349LeuLeu: 10.349 ± 0.204
2.461LeuMet: 2.461 ± 0.081
3.9LeuAsn: 3.9 ± 0.1
4.121LeuPro: 4.121 ± 0.104
2.615LeuGln: 2.615 ± 0.09
5.3LeuArg: 5.3 ± 0.147
8.632LeuSer: 8.632 ± 0.157
4.91LeuThr: 4.91 ± 0.13
7.15LeuVal: 7.15 ± 0.14
0.909LeuTrp: 0.909 ± 0.054
2.842LeuTyr: 2.842 ± 0.096
0.0LeuXaa: 0.0 ± 0.0
Met
1.866MetAla: 1.866 ± 0.077
0.139MetCys: 0.139 ± 0.015
1.245MetAsp: 1.245 ± 0.058
1.767MetGlu: 1.767 ± 0.07
0.922MetPhe: 0.922 ± 0.054
1.885MetGly: 1.885 ± 0.072
0.347MetHis: 0.347 ± 0.03
2.237MetIle: 2.237 ± 0.08
2.484MetLys: 2.484 ± 0.081
2.335MetLeu: 2.335 ± 0.078
0.709MetMet: 0.709 ± 0.048
1.349MetAsn: 1.349 ± 0.062
0.922MetPro: 0.922 ± 0.047
0.552MetGln: 0.552 ± 0.036
1.621MetArg: 1.621 ± 0.062
1.746MetSer: 1.746 ± 0.06
1.365MetThr: 1.365 ± 0.064
1.722MetVal: 1.722 ± 0.073
0.213MetTrp: 0.213 ± 0.025
0.68MetTyr: 0.68 ± 0.044
0.0MetXaa: 0.0 ± 0.0
Asn
2.445AsnAla: 2.445 ± 0.087
0.403AsnCys: 0.403 ± 0.034
1.813AsnAsp: 1.813 ± 0.069
2.623AsnGlu: 2.623 ± 0.077
1.695AsnPhe: 1.695 ± 0.078
2.887AsnGly: 2.887 ± 0.104
0.645AsnHis: 0.645 ± 0.042
2.772AsnIle: 2.772 ± 0.083
1.799AsnLys: 1.799 ± 0.072
4.081AsnLeu: 4.081 ± 0.106
0.936AsnMet: 0.936 ± 0.044
1.293AsnAsn: 1.293 ± 0.065
2.093AsnPro: 2.093 ± 0.074
0.882AsnGln: 0.882 ± 0.048
2.194AsnArg: 2.194 ± 0.082
2.804AsnSer: 2.804 ± 0.108
1.738AsnThr: 1.738 ± 0.066
2.684AsnVal: 2.684 ± 0.086
0.52AsnTrp: 0.52 ± 0.038
1.541AsnTyr: 1.541 ± 0.07
0.0AsnXaa: 0.0 ± 0.0
Pro
2.314ProAla: 2.314 ± 0.088
0.259ProCys: 0.259 ± 0.026
2.506ProAsp: 2.506 ± 0.092
3.62ProGlu: 3.62 ± 0.091
2.098ProPhe: 2.098 ± 0.07
2.94ProGly: 2.94 ± 0.102
0.512ProHis: 0.512 ± 0.035
2.562ProIle: 2.562 ± 0.087
1.671ProLys: 1.671 ± 0.071
3.593ProLeu: 3.593 ± 0.101
0.784ProMet: 0.784 ± 0.048
1.248ProAsn: 1.248 ± 0.054
1.144ProPro: 1.144 ± 0.066
0.954ProGln: 0.954 ± 0.054
1.37ProArg: 1.37 ± 0.061
2.778ProSer: 2.778 ± 0.094
1.786ProThr: 1.786 ± 0.066
3.346ProVal: 3.346 ± 0.11
0.456ProTrp: 0.456 ± 0.034
1.474ProTyr: 1.474 ± 0.066
0.0ProXaa: 0.0 ± 0.0
Gln
1.424GlnAla: 1.424 ± 0.059
0.203GlnCys: 0.203 ± 0.024
1.045GlnAsp: 1.045 ± 0.06
1.911GlnGlu: 1.911 ± 0.079
1.002GlnPhe: 1.002 ± 0.048
1.514GlnGly: 1.514 ± 0.07
0.355GlnHis: 0.355 ± 0.029
1.941GlnIle: 1.941 ± 0.07
1.469GlnLys: 1.469 ± 0.06
2.391GlnLeu: 2.391 ± 0.089
0.68GlnMet: 0.68 ± 0.044
0.978GlnAsn: 0.978 ± 0.05
0.725GlnPro: 0.725 ± 0.043
0.613GlnGln: 0.613 ± 0.041
1.36GlnArg: 1.36 ± 0.072
1.533GlnSer: 1.533 ± 0.072
1.066GlnThr: 1.066 ± 0.054
1.589GlnVal: 1.589 ± 0.068
0.277GlnTrp: 0.277 ± 0.028
0.712GlnTyr: 0.712 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
3.258ArgAla: 3.258 ± 0.093
0.419ArgCys: 0.419 ± 0.037
3.002ArgAsp: 3.002 ± 0.091
4.83ArgGlu: 4.83 ± 0.127
2.863ArgPhe: 2.863 ± 0.083
3.226ArgGly: 3.226 ± 0.101
0.786ArgHis: 0.786 ± 0.045
4.684ArgIle: 4.684 ± 0.124
3.543ArgLys: 3.543 ± 0.098
5.665ArgLeu: 5.665 ± 0.15
1.437ArgMet: 1.437 ± 0.069
2.25ArgAsn: 2.25 ± 0.074
1.722ArgPro: 1.722 ± 0.073
1.274ArgGln: 1.274 ± 0.059
3.33ArgArg: 3.33 ± 0.103
3.45ArgSer: 3.45 ± 0.124
2.519ArgThr: 2.519 ± 0.084
4.017ArgVal: 4.017 ± 0.098
0.517ArgTrp: 0.517 ± 0.038
1.97ArgTyr: 1.97 ± 0.081
0.0ArgXaa: 0.0 ± 0.0
Ser
4.473SerAla: 4.473 ± 0.11
0.653SerCys: 0.653 ± 0.049
3.801SerAsp: 3.801 ± 0.13
5.233SerGlu: 5.233 ± 0.13
4.143SerPhe: 4.143 ± 0.117
6.169SerGly: 6.169 ± 0.144
1.258SerHis: 1.258 ± 0.059
5.569SerIle: 5.569 ± 0.123
4.02SerLys: 4.02 ± 0.116
8.064SerLeu: 8.064 ± 0.159
1.951SerMet: 1.951 ± 0.079
2.655SerAsn: 2.655 ± 0.082
2.722SerPro: 2.722 ± 0.08
1.607SerGln: 1.607 ± 0.064
4.215SerArg: 4.215 ± 0.112
5.697SerSer: 5.697 ± 0.155
3.234SerThr: 3.234 ± 0.096
5.59SerVal: 5.59 ± 0.142
0.853SerTrp: 0.853 ± 0.057
2.423SerTyr: 2.423 ± 0.085
0.0SerXaa: 0.0 ± 0.0
Thr
3.548ThrAla: 3.548 ± 0.119
0.315ThrCys: 0.315 ± 0.033
2.626ThrAsp: 2.626 ± 0.079
3.271ThrGlu: 3.271 ± 0.103
2.391ThrPhe: 2.391 ± 0.098
4.316ThrGly: 4.316 ± 0.111
0.757ThrHis: 0.757 ± 0.045
3.913ThrIle: 3.913 ± 0.106
2.221ThrLys: 2.221 ± 0.069
5.172ThrLeu: 5.172 ± 0.126
1.317ThrMet: 1.317 ± 0.07
1.725ThrAsn: 1.725 ± 0.061
2.141ThrPro: 2.141 ± 0.078
0.888ThrGln: 0.888 ± 0.054
2.282ThrArg: 2.282 ± 0.076
3.394ThrSer: 3.394 ± 0.101
2.538ThrThr: 2.538 ± 0.102
4.169ThrVal: 4.169 ± 0.123
0.56ThrTrp: 0.56 ± 0.052
1.714ThrTyr: 1.714 ± 0.068
0.0ThrXaa: 0.0 ± 0.0
Val
5.249ValAla: 5.249 ± 0.131
0.645ValCys: 0.645 ± 0.044
4.428ValAsp: 4.428 ± 0.105
5.569ValGlu: 5.569 ± 0.132
4.14ValPhe: 4.14 ± 0.106
4.966ValGly: 4.966 ± 0.126
1.045ValHis: 1.045 ± 0.049
5.611ValIle: 5.611 ± 0.118
4.111ValLys: 4.111 ± 0.115
7.358ValLeu: 7.358 ± 0.15
1.773ValMet: 1.773 ± 0.073
2.676ValAsn: 2.676 ± 0.098
2.836ValPro: 2.836 ± 0.086
1.685ValGln: 1.685 ± 0.08
3.879ValArg: 3.879 ± 0.11
5.825ValSer: 5.825 ± 0.144
3.887ValThr: 3.887 ± 0.119
6.041ValVal: 6.041 ± 0.135
0.698ValTrp: 0.698 ± 0.044
2.335ValTyr: 2.335 ± 0.091
0.0ValXaa: 0.0 ± 0.0
Trp
0.602TrpAla: 0.602 ± 0.04
0.085TrpCys: 0.085 ± 0.015
0.538TrpAsp: 0.538 ± 0.042
0.765TrpGlu: 0.765 ± 0.044
0.504TrpPhe: 0.504 ± 0.034
0.696TrpGly: 0.696 ± 0.043
0.195TrpHis: 0.195 ± 0.027
0.845TrpIle: 0.845 ± 0.049
0.765TrpLys: 0.765 ± 0.052
1.13TrpLeu: 1.13 ± 0.063
0.264TrpMet: 0.264 ± 0.025
0.522TrpAsn: 0.522 ± 0.041
0.376TrpPro: 0.376 ± 0.032
0.363TrpGln: 0.363 ± 0.036
0.483TrpArg: 0.483 ± 0.037
0.736TrpSer: 0.736 ± 0.049
0.562TrpThr: 0.562 ± 0.048
0.637TrpVal: 0.637 ± 0.043
0.163TrpTrp: 0.163 ± 0.021
0.4TrpTyr: 0.4 ± 0.036
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.13TyrAla: 2.13 ± 0.072
0.419TyrCys: 0.419 ± 0.035
2.117TyrAsp: 2.117 ± 0.08
2.338TyrGlu: 2.338 ± 0.084
1.877TyrPhe: 1.877 ± 0.073
2.588TyrGly: 2.588 ± 0.091
0.592TyrHis: 0.592 ± 0.041
2.199TyrIle: 2.199 ± 0.083
1.336TyrLys: 1.336 ± 0.062
3.583TyrLeu: 3.583 ± 0.094
0.773TyrMet: 0.773 ± 0.047
1.242TyrAsn: 1.242 ± 0.055
1.378TyrPro: 1.378 ± 0.058
0.813TyrGln: 0.813 ± 0.05
2.058TyrArg: 2.058 ± 0.077
2.951TyrSer: 2.951 ± 0.088
1.557TyrThr: 1.557 ± 0.073
2.383TyrVal: 2.383 ± 0.09
0.392TyrTrp: 0.392 ± 0.038
1.543TyrTyr: 1.543 ± 0.067
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1273 proteins (375127 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski