DPANN group archaeon LC1Nh

Taxonomy: cellular organisms; Archaea; DPANN group; Candidatus Nanohaloarchaeota; Candidatus Nanohalobia; Candidatus Nanohalobiales; Candidatus Nanohalobiaceae; Candidatus Nanohalobium

Average proteome isoelectric point is 5.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1162 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q0UFP3|A0A5Q0UFP3_9ARCH Uncharacterized protein OS=DPANN group archaeon LC1Nh OX=2565781 GN=LC1Nh_0518 PE=4 SV=1
MM1 pKa = 7.16KK2 pKa = 10.23RR3 pKa = 11.84KK4 pKa = 9.35IGSKK8 pKa = 9.64IGLLIVFAAALTSVAAAQATINYY31 pKa = 8.28DD32 pKa = 3.65YY33 pKa = 11.41NVDD36 pKa = 3.84DD37 pKa = 4.51VEE39 pKa = 5.26VMAFNCLNSTDD50 pKa = 4.06CSNVGDD56 pKa = 3.97FGGNILDD63 pKa = 5.05SGTTSNGEE71 pKa = 3.59ITIDD75 pKa = 3.79YY76 pKa = 8.9PAEE79 pKa = 4.22LQDD82 pKa = 3.41HH83 pKa = 6.99GYY85 pKa = 10.81AVYY88 pKa = 9.84HH89 pKa = 5.9VSPGYY94 pKa = 10.9VPVEE98 pKa = 4.65AYY100 pKa = 10.52ADD102 pKa = 3.87WSCGGSGSCGGNTTDD117 pKa = 3.78VQFEE121 pKa = 4.68KK122 pKa = 10.34ISQCSATVDD131 pKa = 3.73SFSVTNDD138 pKa = 2.66VYY140 pKa = 11.45ANEE143 pKa = 4.47PLTVDD148 pKa = 3.49VSATAEE154 pKa = 4.35STVSSAFEE162 pKa = 3.54EE163 pKa = 4.69AGPVDD168 pKa = 4.7HH169 pKa = 7.17VPEE172 pKa = 4.78AYY174 pKa = 9.5KK175 pKa = 10.59DD176 pKa = 3.47QYY178 pKa = 11.75YY179 pKa = 10.63SANTNISLEE188 pKa = 4.26VTDD191 pKa = 3.81TDD193 pKa = 3.97GNPVHH198 pKa = 6.57TAEE201 pKa = 4.11QQKK204 pKa = 10.23NIYY207 pKa = 9.09MNEE210 pKa = 3.81TDD212 pKa = 3.3QVEE215 pKa = 4.65FGWTPSKK222 pKa = 11.01SEE224 pKa = 4.03NYY226 pKa = 8.26TVQASTEE233 pKa = 4.27VIDD236 pKa = 4.44PQCSSTNILSTDD248 pKa = 3.4KK249 pKa = 11.24EE250 pKa = 4.63PITVHH255 pKa = 5.9EE256 pKa = 4.41EE257 pKa = 3.93RR258 pKa = 11.84PQNEE262 pKa = 5.22YY263 pKa = 9.33YY264 pKa = 10.65TLINDD269 pKa = 5.35LDD271 pKa = 3.85IQDD274 pKa = 4.1NHH276 pKa = 6.78PKK278 pKa = 9.26ATQQQTFTYY287 pKa = 10.31SKK289 pKa = 9.9ISNYY293 pKa = 10.36ADD295 pKa = 3.13NDD297 pKa = 3.55YY298 pKa = 11.51DD299 pKa = 3.66KK300 pKa = 10.76TAVKK304 pKa = 9.97TDD306 pKa = 2.98VNYY309 pKa = 9.54TVRR312 pKa = 11.84DD313 pKa = 3.37SDD315 pKa = 3.74GQEE318 pKa = 3.96VNFSTEE324 pKa = 3.69QLPANQNEE332 pKa = 3.82QDD334 pKa = 3.56AEE336 pKa = 4.32SFSFSWTPKK345 pKa = 9.23SAGTYY350 pKa = 6.33TVEE353 pKa = 4.12VAGEE357 pKa = 4.23SVQTEE362 pKa = 4.68GEE364 pKa = 4.2NPPTSPDD371 pKa = 3.43TANLEE376 pKa = 3.62IDD378 pKa = 3.75VAEE381 pKa = 4.12EE382 pKa = 4.09PKK384 pKa = 10.35WKK386 pKa = 10.85VEE388 pKa = 4.03FNVVDD393 pKa = 3.83TNGDD397 pKa = 3.59PVNNAEE403 pKa = 4.29VKK405 pKa = 10.74INDD408 pKa = 3.85RR409 pKa = 11.84TNQTDD414 pKa = 3.58SNGNTEE420 pKa = 3.83ITKK423 pKa = 8.78LTKK426 pKa = 10.25GSYY429 pKa = 8.61TFNVSKK435 pKa = 9.66TGYY438 pKa = 5.27TTSSGSLTIEE448 pKa = 4.26STTSDD453 pKa = 4.03PLQKK457 pKa = 10.58SVVIQNNNTAPKK469 pKa = 10.69VLDD472 pKa = 3.64IPNKK476 pKa = 10.26KK477 pKa = 9.36IVEE480 pKa = 4.33GDD482 pKa = 3.52TNNDD486 pKa = 3.0INLDD490 pKa = 3.93NYY492 pKa = 10.7VSDD495 pKa = 4.14QQDD498 pKa = 3.49LASEE502 pKa = 4.41ISWTSNGAEE511 pKa = 4.32DD512 pKa = 4.81NQNLDD517 pKa = 3.45VNIDD521 pKa = 3.79PDD523 pKa = 3.75THH525 pKa = 6.76DD526 pKa = 3.8VNITSTSGWTGTEE539 pKa = 4.23SITFTATDD547 pKa = 3.43TAGATDD553 pKa = 4.22SDD555 pKa = 4.51SMTVTVTEE563 pKa = 4.63KK564 pKa = 9.73NTAPTVSEE572 pKa = 4.32IPDD575 pKa = 3.35VTLDD579 pKa = 3.76EE580 pKa = 4.86DD581 pKa = 3.92TTEE584 pKa = 3.76YY585 pKa = 11.12DD586 pKa = 3.54YY587 pKa = 12.17VNLVNFVSDD596 pKa = 5.07AEE598 pKa = 4.37STYY601 pKa = 11.56NNLDD605 pKa = 3.06VSIDD609 pKa = 3.68VSSDD613 pKa = 2.88KK614 pKa = 11.14CGVSIDD620 pKa = 3.92EE621 pKa = 4.44NEE623 pKa = 3.81IDD625 pKa = 4.27IAPNTNFHH633 pKa = 5.71GTCTVTVQATDD644 pKa = 3.8PYY646 pKa = 11.46GATTSNNFEE655 pKa = 3.97ITVNPVNDD663 pKa = 4.07PPTITSNPQTTVDD676 pKa = 4.84AYY678 pKa = 8.6TQYY681 pKa = 11.28YY682 pKa = 8.39YY683 pKa = 10.67QLSVSDD689 pKa = 4.71PDD691 pKa = 3.32NGAASFTYY699 pKa = 9.79TKK701 pKa = 10.32EE702 pKa = 3.7QGPANMTINQNGEE715 pKa = 4.39VTWTPTNNNADD726 pKa = 3.88NSPHH730 pKa = 5.95QVTLNVSDD738 pKa = 4.03GTNSTTQSYY747 pKa = 11.22SLTVNKK753 pKa = 10.13APKK756 pKa = 9.54NQIPHH761 pKa = 6.82PEE763 pKa = 3.52ITVNKK768 pKa = 8.71TKK770 pKa = 10.69GKK772 pKa = 10.79APLTVNFNASNSADD786 pKa = 3.62PDD788 pKa = 3.96GSITQYY794 pKa = 10.35NWSFGEE800 pKa = 4.13GGKK803 pKa = 10.78ANGIEE808 pKa = 4.14TNYY811 pKa = 10.62TYY813 pKa = 8.96TQPGNYY819 pKa = 7.65TATLTLTDD827 pKa = 4.9DD828 pKa = 4.08NGSTATEE835 pKa = 4.04SVRR838 pKa = 11.84IEE840 pKa = 3.94VTGNNAPSFTSTSFKK855 pKa = 8.51TTATVNQQYY864 pKa = 8.89TYY866 pKa = 10.69DD867 pKa = 3.82AQATDD872 pKa = 3.8PDD874 pKa = 4.83GDD876 pKa = 3.81TLEE879 pKa = 4.61YY880 pKa = 10.84SLNTAPQSMSINNNTGKK897 pKa = 8.47ITWTPSNTGEE907 pKa = 4.24YY908 pKa = 9.65KK909 pKa = 10.63VEE911 pKa = 3.91IQVSDD916 pKa = 3.72GTDD919 pKa = 3.1TAIQSYY925 pKa = 10.47NITVSSNSDD934 pKa = 3.08NDD936 pKa = 3.9GGGDD940 pKa = 3.85DD941 pKa = 4.51EE942 pKa = 6.23DD943 pKa = 4.78SGGSSSGGGGGGGGGGFGGSFTTTDD968 pKa = 3.42EE969 pKa = 4.32EE970 pKa = 4.45EE971 pKa = 4.22EE972 pKa = 4.0EE973 pKa = 4.44SIEE976 pKa = 4.52EE977 pKa = 4.52RR978 pKa = 11.84IQTGDD983 pKa = 3.59NEE985 pKa = 4.18VDD987 pKa = 3.65LDD989 pKa = 4.09IEE991 pKa = 4.38PGIDD995 pKa = 3.38DD996 pKa = 4.79SYY998 pKa = 11.95NLTFNVSEE1006 pKa = 4.5DD1007 pKa = 3.86TNITTSVTGNVSEE1020 pKa = 4.42IVSVPANFTAKK1031 pKa = 10.29KK1032 pKa = 10.51GEE1034 pKa = 4.27NNLPLQINGSVPGNYY1049 pKa = 8.89TGNLTLQWNNQTLNIPLNLNIEE1071 pKa = 4.35DD1072 pKa = 4.31TGASDD1077 pKa = 3.72YY1078 pKa = 11.56SVAILHH1084 pKa = 6.68KK1085 pKa = 9.13STADD1089 pKa = 3.23EE1090 pKa = 4.68RR1091 pKa = 11.84ISYY1094 pKa = 9.44DD1095 pKa = 3.17VKK1097 pKa = 10.9LWDD1100 pKa = 3.88EE1101 pKa = 4.48EE1102 pKa = 4.59KK1103 pKa = 10.54PDD1105 pKa = 3.51TSNQVSITTSVIDD1118 pKa = 3.64SEE1120 pKa = 4.33GDD1122 pKa = 3.08EE1123 pKa = 4.42VYY1125 pKa = 10.91RR1126 pKa = 11.84NTEE1129 pKa = 3.54TEE1131 pKa = 3.86RR1132 pKa = 11.84IYY1134 pKa = 11.3DD1135 pKa = 3.57VLVRR1139 pKa = 11.84NYY1141 pKa = 7.73RR1142 pKa = 11.84TSILPEE1148 pKa = 3.85GEE1150 pKa = 4.25YY1151 pKa = 10.33TVTATVTDD1159 pKa = 4.21DD1160 pKa = 3.88GEE1162 pKa = 4.4EE1163 pKa = 4.2FTGSRR1168 pKa = 11.84TIQLGPSEE1176 pKa = 4.7TITGQFTSSNSLGSLFQDD1194 pKa = 4.07IIDD1197 pKa = 4.58SISNLFF1203 pKa = 3.51

Molecular weight: 129.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q0UG78|A0A5Q0UG78_9ARCH ABC-2 type transport system ATP-binding protein OS=DPANN group archaeon LC1Nh OX=2565781 GN=LC1Nh_0707 PE=4 SV=1
MM1 pKa = 6.48NTKK4 pKa = 10.06NGRR7 pKa = 11.84KK8 pKa = 7.59RR9 pKa = 11.84TPLRR13 pKa = 11.84SRR15 pKa = 11.84VRR17 pKa = 11.84STALRR22 pKa = 11.84TRR24 pKa = 11.84TRR26 pKa = 11.84SNTSSRR32 pKa = 11.84RR33 pKa = 11.84HH34 pKa = 5.91RR35 pKa = 11.84SRR37 pKa = 11.84SSNSSLTKK45 pKa = 10.14IEE47 pKa = 4.09VLQDD51 pKa = 3.13

Molecular weight: 5.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1162

0

1162

293371

45

1499

252.5

28.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.045 ± 0.065

0.648 ± 0.026

7.212 ± 0.094

11.403 ± 0.155

3.968 ± 0.061

7.006 ± 0.079

1.674 ± 0.031

6.33 ± 0.064

6.339 ± 0.095

8.179 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.312 ± 0.04

4.652 ± 0.072

3.258 ± 0.039

3.637 ± 0.055

4.38 ± 0.05

6.912 ± 0.092

5.307 ± 0.083

6.723 ± 0.064

0.879 ± 0.025

3.136 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski