Amino acid dipepetide frequency for DPANN group archaeon LC1Nh

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.912AlaAla: 4.912 ± 0.156
0.372AlaCys: 0.372 ± 0.04
4.135AlaAsp: 4.135 ± 0.122
7.475AlaGlu: 7.475 ± 0.189
2.342AlaPhe: 2.342 ± 0.093
4.677AlaGly: 4.677 ± 0.134
0.828AlaHis: 0.828 ± 0.05
3.354AlaIle: 3.354 ± 0.128
3.15AlaLys: 3.15 ± 0.119
5.123AlaLeu: 5.123 ± 0.184
1.248AlaMet: 1.248 ± 0.068
2.045AlaAsn: 2.045 ± 0.094
1.735AlaPro: 1.735 ± 0.088
1.991AlaGln: 1.991 ± 0.078
2.543AlaArg: 2.543 ± 0.089
4.073AlaSer: 4.073 ± 0.138
3.058AlaThr: 3.058 ± 0.131
5.089AlaVal: 5.089 ± 0.165
0.46AlaTrp: 0.46 ± 0.042
1.841AlaTyr: 1.841 ± 0.084
0.0AlaXaa: 0.0 ± 0.0
Cys
0.31CysAla: 0.31 ± 0.037
0.037CysCys: 0.037 ± 0.011
0.498CysAsp: 0.498 ± 0.045
0.532CysGlu: 0.532 ± 0.047
0.232CysPhe: 0.232 ± 0.029
0.866CysGly: 0.866 ± 0.063
0.109CysHis: 0.109 ± 0.02
0.344CysIle: 0.344 ± 0.037
0.327CysLys: 0.327 ± 0.031
0.457CysLeu: 0.457 ± 0.037
0.164CysMet: 0.164 ± 0.025
0.276CysAsn: 0.276 ± 0.033
0.372CysPro: 0.372 ± 0.033
0.225CysGln: 0.225 ± 0.029
0.392CysArg: 0.392 ± 0.042
0.528CysSer: 0.528 ± 0.049
0.259CysThr: 0.259 ± 0.035
0.32CysVal: 0.32 ± 0.038
0.075CysTrp: 0.075 ± 0.017
0.16CysTyr: 0.16 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
4.176AspAla: 4.176 ± 0.131
0.528AspCys: 0.528 ± 0.039
4.527AspAsp: 4.527 ± 0.151
7.952AspGlu: 7.952 ± 0.19
3.569AspPhe: 3.569 ± 0.123
4.953AspGly: 4.953 ± 0.17
1.22AspHis: 1.22 ± 0.058
5.004AspIle: 5.004 ± 0.132
4.322AspLys: 4.322 ± 0.121
6.214AspLeu: 6.214 ± 0.185
1.766AspMet: 1.766 ± 0.087
2.972AspAsn: 2.972 ± 0.116
2.301AspPro: 2.301 ± 0.087
2.567AspGln: 2.567 ± 0.105
3.33AspArg: 3.33 ± 0.114
4.929AspSer: 4.929 ± 0.162
3.668AspThr: 3.668 ± 0.112
4.571AspVal: 4.571 ± 0.158
0.835AspTrp: 0.835 ± 0.058
2.713AspTyr: 2.713 ± 0.101
0.0AspXaa: 0.0 ± 0.0
Glu
6.667GluAla: 6.667 ± 0.181
0.641GluCys: 0.641 ± 0.048
9.118GluAsp: 9.118 ± 0.221
15.472GluGlu: 15.472 ± 0.401
4.182GluPhe: 4.182 ± 0.127
6.511GluGly: 6.511 ± 0.185
1.759GluHis: 1.759 ± 0.081
7.738GluIle: 7.738 ± 0.189
9.807GluLys: 9.807 ± 0.241
8.856GluLeu: 8.856 ± 0.223
2.856GluMet: 2.856 ± 0.124
6.541GluAsn: 6.541 ± 0.156
2.652GluPro: 2.652 ± 0.109
3.296GluGln: 3.296 ± 0.134
4.479GluArg: 4.479 ± 0.129
5.536GluSer: 5.536 ± 0.153
5.577GluThr: 5.577 ± 0.172
7.976GluVal: 7.976 ± 0.199
1.029GluTrp: 1.029 ± 0.069
3.337GluTyr: 3.337 ± 0.113
0.0GluXaa: 0.0 ± 0.0
Phe
2.011PheAla: 2.011 ± 0.092
0.28PheCys: 0.28 ± 0.031
3.01PheAsp: 3.01 ± 0.104
4.046PheGlu: 4.046 ± 0.126
1.67PhePhe: 1.67 ± 0.091
2.679PheGly: 2.679 ± 0.113
0.74PheHis: 0.74 ± 0.047
2.311PheIle: 2.311 ± 0.103
2.055PheLys: 2.055 ± 0.092
3.61PheLeu: 3.61 ± 0.126
1.009PheMet: 1.009 ± 0.061
1.95PheAsn: 1.95 ± 0.086
1.275PhePro: 1.275 ± 0.071
1.694PheGln: 1.694 ± 0.064
1.67PheArg: 1.67 ± 0.083
3.34PheSer: 3.34 ± 0.123
2.376PheThr: 2.376 ± 0.102
2.314PheVal: 2.314 ± 0.104
0.334PheTrp: 0.334 ± 0.03
1.309PheTyr: 1.309 ± 0.06
0.0PheXaa: 0.0 ± 0.0
Gly
3.985GlyAla: 3.985 ± 0.131
0.501GlyCys: 0.501 ± 0.042
5.103GlyAsp: 5.103 ± 0.159
7.632GlyGlu: 7.632 ± 0.201
3.497GlyPhe: 3.497 ± 0.119
4.895GlyGly: 4.895 ± 0.197
1.183GlyHis: 1.183 ± 0.063
4.38GlyIle: 4.38 ± 0.138
4.377GlyLys: 4.377 ± 0.136
5.87GlyLeu: 5.87 ± 0.141
1.585GlyMet: 1.585 ± 0.075
3.334GlyAsn: 3.334 ± 0.141
1.578GlyPro: 1.578 ± 0.073
2.175GlyGln: 2.175 ± 0.083
3.15GlyArg: 3.15 ± 0.103
5.229GlySer: 5.229 ± 0.176
3.777GlyThr: 3.777 ± 0.132
4.714GlyVal: 4.714 ± 0.133
0.62GlyTrp: 0.62 ± 0.048
2.475GlyTyr: 2.475 ± 0.088
0.0GlyXaa: 0.0 ± 0.0
His
0.992HisAla: 0.992 ± 0.059
0.17HisCys: 0.17 ± 0.028
1.111HisAsp: 1.111 ± 0.065
1.653HisGlu: 1.653 ± 0.085
0.641HisPhe: 0.641 ± 0.043
1.234HisGly: 1.234 ± 0.067
0.447HisHis: 0.447 ± 0.042
1.04HisIle: 1.04 ± 0.056
0.825HisLys: 0.825 ± 0.055
1.401HisLeu: 1.401 ± 0.067
0.361HisMet: 0.361 ± 0.032
0.654HisAsn: 0.654 ± 0.046
0.849HisPro: 0.849 ± 0.059
0.651HisGln: 0.651 ± 0.051
0.907HisArg: 0.907 ± 0.062
1.128HisSer: 1.128 ± 0.063
0.845HisThr: 0.845 ± 0.05
1.098HisVal: 1.098 ± 0.054
0.222HisTrp: 0.222 ± 0.025
0.511HisTyr: 0.511 ± 0.039
0.0HisXaa: 0.0 ± 0.0
Ile
3.886IleAla: 3.886 ± 0.121
0.392IleCys: 0.392 ± 0.033
4.793IleAsp: 4.793 ± 0.139
7.117IleGlu: 7.117 ± 0.163
2.396IlePhe: 2.396 ± 0.099
5.103IleGly: 5.103 ± 0.188
1.046IleHis: 1.046 ± 0.061
3.63IleIle: 3.63 ± 0.115
3.525IleLys: 3.525 ± 0.127
5.225IleLeu: 5.225 ± 0.162
1.193IleMet: 1.193 ± 0.06
2.73IleAsn: 2.73 ± 0.111
2.485IlePro: 2.485 ± 0.089
2.584IleGln: 2.584 ± 0.099
2.826IleArg: 2.826 ± 0.097
4.905IleSer: 4.905 ± 0.135
3.419IleThr: 3.419 ± 0.135
3.76IleVal: 3.76 ± 0.133
0.409IleTrp: 0.409 ± 0.045
1.871IleTyr: 1.871 ± 0.081
0.0IleXaa: 0.0 ± 0.0
Lys
4.179LysAla: 4.179 ± 0.128
0.419LysCys: 0.419 ± 0.044
4.121LysAsp: 4.121 ± 0.138
7.196LysGlu: 7.196 ± 0.195
2.161LysPhe: 2.161 ± 0.089
3.576LysGly: 3.576 ± 0.128
1.159LysHis: 1.159 ± 0.067
4.793LysIle: 4.793 ± 0.147
4.54LysLys: 4.54 ± 0.173
5.331LysLeu: 5.331 ± 0.146
1.646LysMet: 1.646 ± 0.084
2.925LysAsn: 2.925 ± 0.108
2.178LysPro: 2.178 ± 0.098
2.608LysGln: 2.608 ± 0.118
2.604LysArg: 2.604 ± 0.1
3.402LysSer: 3.402 ± 0.12
3.49LysThr: 3.49 ± 0.109
4.503LysVal: 4.503 ± 0.14
0.641LysTrp: 0.641 ± 0.049
1.922LysTyr: 1.922 ± 0.095
0.0LysXaa: 0.0 ± 0.0
Leu
5.157LeuAla: 5.157 ± 0.152
0.399LeuCys: 0.399 ± 0.037
6.357LeuAsp: 6.357 ± 0.153
10.059LeuGlu: 10.059 ± 0.236
2.689LeuPhe: 2.689 ± 0.108
5.982LeuGly: 5.982 ± 0.163
1.363LeuHis: 1.363 ± 0.063
4.919LeuIle: 4.919 ± 0.155
5.181LeuLys: 5.181 ± 0.147
6.091LeuLeu: 6.091 ± 0.191
1.803LeuMet: 1.803 ± 0.075
3.787LeuAsn: 3.787 ± 0.133
2.618LeuPro: 2.618 ± 0.093
2.833LeuGln: 2.833 ± 0.115
3.804LeuArg: 3.804 ± 0.132
5.958LeuSer: 5.958 ± 0.157
4.585LeuThr: 4.585 ± 0.154
5.324LeuVal: 5.324 ± 0.147
0.593LeuTrp: 0.593 ± 0.047
2.291LeuTyr: 2.291 ± 0.092
0.0LeuXaa: 0.0 ± 0.0
Met
1.585MetAla: 1.585 ± 0.076
0.099MetCys: 0.099 ± 0.017
1.732MetAsp: 1.732 ± 0.081
2.239MetGlu: 2.239 ± 0.094
0.784MetPhe: 0.784 ± 0.054
1.306MetGly: 1.306 ± 0.066
0.392MetHis: 0.392 ± 0.032
1.459MetIle: 1.459 ± 0.08
1.895MetLys: 1.895 ± 0.085
1.803MetLeu: 1.803 ± 0.088
0.62MetMet: 0.62 ± 0.05
1.166MetAsn: 1.166 ± 0.064
0.968MetPro: 0.968 ± 0.059
0.856MetGln: 0.856 ± 0.05
1.043MetArg: 1.043 ± 0.06
1.738MetSer: 1.738 ± 0.08
1.282MetThr: 1.282 ± 0.069
1.36MetVal: 1.36 ± 0.072
0.143MetTrp: 0.143 ± 0.022
0.654MetTyr: 0.654 ± 0.043
0.0MetXaa: 0.0 ± 0.0
Asn
2.539AsnAla: 2.539 ± 0.101
0.423AsnCys: 0.423 ± 0.037
2.55AsnAsp: 2.55 ± 0.089
3.327AsnGlu: 3.327 ± 0.11
2.072AsnPhe: 2.072 ± 0.092
3.255AsnGly: 3.255 ± 0.143
0.839AsnHis: 0.839 ± 0.065
3.446AsnIle: 3.446 ± 0.132
2.454AsnLys: 2.454 ± 0.096
4.404AsnLeu: 4.404 ± 0.158
1.173AsnMet: 1.173 ± 0.066
2.141AsnAsn: 2.141 ± 0.114
2.366AsnPro: 2.366 ± 0.115
2.141AsnGln: 2.141 ± 0.091
2.038AsnArg: 2.038 ± 0.078
3.698AsnSer: 3.698 ± 0.129
2.734AsnThr: 2.734 ± 0.116
2.471AsnVal: 2.471 ± 0.115
0.539AsnTrp: 0.539 ± 0.05
1.909AsnTyr: 1.909 ± 0.082
0.0AsnXaa: 0.0 ± 0.0
Pro
1.882ProAla: 1.882 ± 0.083
0.177ProCys: 0.177 ± 0.025
2.737ProAsp: 2.737 ± 0.106
5.007ProGlu: 5.007 ± 0.147
1.275ProPhe: 1.275 ± 0.07
2.655ProGly: 2.655 ± 0.101
0.668ProHis: 0.668 ± 0.051
1.568ProIle: 1.568 ± 0.073
1.657ProLys: 1.657 ± 0.081
2.219ProLeu: 2.219 ± 0.1
0.631ProMet: 0.631 ± 0.039
1.282ProAsn: 1.282 ± 0.07
0.968ProPro: 0.968 ± 0.062
1.231ProGln: 1.231 ± 0.069
1.145ProArg: 1.145 ± 0.065
2.205ProSer: 2.205 ± 0.095
1.449ProThr: 1.449 ± 0.07
2.604ProVal: 2.604 ± 0.094
0.252ProTrp: 0.252 ± 0.032
0.965ProTyr: 0.965 ± 0.067
0.0ProXaa: 0.0 ± 0.0
Gln
2.021GlnAla: 2.021 ± 0.079
0.191GlnCys: 0.191 ± 0.025
2.516GlnAsp: 2.516 ± 0.087
4.332GlnGlu: 4.332 ± 0.146
1.135GlnPhe: 1.135 ± 0.058
2.28GlnGly: 2.28 ± 0.095
0.535GlnHis: 0.535 ± 0.043
2.301GlnIle: 2.301 ± 0.097
2.86GlnLys: 2.86 ± 0.113
2.88GlnLeu: 2.88 ± 0.084
1.053GlnMet: 1.053 ± 0.068
2.096GlnAsn: 2.096 ± 0.102
1.213GlnPro: 1.213 ± 0.069
1.513GlnGln: 1.513 ± 0.101
1.65GlnArg: 1.65 ± 0.083
2.209GlnSer: 2.209 ± 0.108
1.94GlnThr: 1.94 ± 0.09
2.383GlnVal: 2.383 ± 0.102
0.239GlnTrp: 0.239 ± 0.03
1.019GlnTyr: 1.019 ± 0.064
0.0GlnXaa: 0.0 ± 0.0
Arg
2.226ArgAla: 2.226 ± 0.096
0.269ArgCys: 0.269 ± 0.035
3.378ArgAsp: 3.378 ± 0.109
5.413ArgGlu: 5.413 ± 0.156
1.841ArgPhe: 1.841 ± 0.083
2.659ArgGly: 2.659 ± 0.096
0.692ArgHis: 0.692 ± 0.049
3.054ArgIle: 3.054 ± 0.102
3.773ArgLys: 3.773 ± 0.122
3.221ArgLeu: 3.221 ± 0.095
1.07ArgMet: 1.07 ± 0.06
2.393ArgAsn: 2.393 ± 0.092
1.132ArgPro: 1.132 ± 0.063
1.442ArgGln: 1.442 ± 0.068
2.008ArgArg: 2.008 ± 0.084
2.553ArgSer: 2.553 ± 0.1
1.963ArgThr: 1.963 ± 0.076
2.921ArgVal: 2.921 ± 0.108
0.351ArgTrp: 0.351 ± 0.035
1.438ArgTyr: 1.438 ± 0.076
0.0ArgXaa: 0.0 ± 0.0
Ser
3.862SerAla: 3.862 ± 0.145
0.412SerCys: 0.412 ± 0.038
4.997SerAsp: 4.997 ± 0.142
7.049SerGlu: 7.049 ± 0.178
3.075SerPhe: 3.075 ± 0.122
5.904SerGly: 5.904 ± 0.187
1.176SerHis: 1.176 ± 0.059
4.506SerIle: 4.506 ± 0.141
3.818SerLys: 3.818 ± 0.125
5.287SerLeu: 5.287 ± 0.155
1.568SerMet: 1.568 ± 0.074
3.133SerAsn: 3.133 ± 0.147
1.997SerPro: 1.997 ± 0.082
2.764SerGln: 2.764 ± 0.105
3.146SerArg: 3.146 ± 0.117
5.614SerSer: 5.614 ± 0.227
3.76SerThr: 3.76 ± 0.143
4.438SerVal: 4.438 ± 0.157
0.658SerTrp: 0.658 ± 0.049
1.96SerTyr: 1.96 ± 0.089
0.0SerXaa: 0.0 ± 0.0
Thr
3.736ThrAla: 3.736 ± 0.162
0.368ThrCys: 0.368 ± 0.038
3.661ThrAsp: 3.661 ± 0.126
5.311ThrGlu: 5.311 ± 0.16
1.776ThrPhe: 1.776 ± 0.079
4.905ThrGly: 4.905 ± 0.154
0.852ThrHis: 0.852 ± 0.055
3.143ThrIle: 3.143 ± 0.127
2.372ThrLys: 2.372 ± 0.089
4.411ThrLeu: 4.411 ± 0.142
0.941ThrMet: 0.941 ± 0.057
1.991ThrAsn: 1.991 ± 0.107
2.154ThrPro: 2.154 ± 0.086
2.011ThrGln: 2.011 ± 0.086
1.929ThrArg: 1.929 ± 0.073
3.855ThrSer: 3.855 ± 0.136
2.959ThrThr: 2.959 ± 0.149
4.687ThrVal: 4.687 ± 0.168
0.372ThrTrp: 0.372 ± 0.034
1.633ThrTyr: 1.633 ± 0.072
0.0ThrXaa: 0.0 ± 0.0
Val
4.08ValAla: 4.08 ± 0.142
0.426ValCys: 0.426 ± 0.04
4.878ValAsp: 4.878 ± 0.144
8.552ValGlu: 8.552 ± 0.188
2.744ValPhe: 2.744 ± 0.1
3.978ValGly: 3.978 ± 0.121
0.982ValHis: 0.982 ± 0.058
3.896ValIle: 3.896 ± 0.14
4.223ValLys: 4.223 ± 0.123
5.812ValLeu: 5.812 ± 0.167
1.418ValMet: 1.418 ± 0.071
3.027ValAsn: 3.027 ± 0.109
2.396ValPro: 2.396 ± 0.11
2.263ValGln: 2.263 ± 0.092
2.918ValArg: 2.918 ± 0.094
4.878ValSer: 4.878 ± 0.168
3.511ValThr: 3.511 ± 0.15
4.636ValVal: 4.636 ± 0.156
0.518ValTrp: 0.518 ± 0.043
2.096ValTyr: 2.096 ± 0.087
0.0ValXaa: 0.0 ± 0.0
Trp
0.535TrpAla: 0.535 ± 0.043
0.058TrpCys: 0.058 ± 0.015
0.59TrpAsp: 0.59 ± 0.044
0.811TrpGlu: 0.811 ± 0.059
0.341TrpPhe: 0.341 ± 0.032
0.484TrpGly: 0.484 ± 0.043
0.208TrpHis: 0.208 ± 0.028
0.573TrpIle: 0.573 ± 0.044
0.682TrpLys: 0.682 ± 0.049
0.685TrpLeu: 0.685 ± 0.047
0.327TrpMet: 0.327 ± 0.033
0.552TrpAsn: 0.552 ± 0.04
0.164TrpPro: 0.164 ± 0.025
0.331TrpGln: 0.331 ± 0.036
0.429TrpArg: 0.429 ± 0.04
0.641TrpSer: 0.641 ± 0.05
0.494TrpThr: 0.494 ± 0.051
0.504TrpVal: 0.504 ± 0.043
0.075TrpTrp: 0.075 ± 0.016
0.307TrpTyr: 0.307 ± 0.032
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.708TyrAla: 1.708 ± 0.084
0.32TyrCys: 0.32 ± 0.029
2.308TyrAsp: 2.308 ± 0.09
2.853TyrGlu: 2.853 ± 0.105
1.261TyrPhe: 1.261 ± 0.071
2.158TyrGly: 2.158 ± 0.079
0.542TyrHis: 0.542 ± 0.045
1.837TyrIle: 1.837 ± 0.083
1.551TyrLys: 1.551 ± 0.076
2.894TyrLeu: 2.894 ± 0.107
0.689TyrMet: 0.689 ± 0.052
1.524TyrAsn: 1.524 ± 0.076
1.179TyrPro: 1.179 ± 0.065
1.193TyrGln: 1.193 ± 0.067
1.786TyrArg: 1.786 ± 0.078
2.703TyrSer: 2.703 ± 0.101
1.922TyrThr: 1.922 ± 0.092
1.558TyrVal: 1.558 ± 0.084
0.426TyrTrp: 0.426 ± 0.037
0.944TyrTyr: 0.944 ± 0.061
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1162 proteins (293371 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski