Faecalibacterium prausnitzii L2-6
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2755 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D4K4R8|D4K4R8_9FIRM CobQ/CobB/MinD/ParA nucleotide binding domain OS=Faecalibacterium prausnitzii L2-6 OX=718252 GN=FP2_09480 PE=4 SV=1
MM1 pKa = 7.21 FTPKK5 pKa = 10.31 NIQGALEE12 pKa = 4.07 EE13 pKa = 5.47 LYY15 pKa = 10.79 DD16 pKa = 4.61 LCDD19 pKa = 3.77 PDD21 pKa = 5.15 YY22 pKa = 10.8 MVDD25 pKa = 3.24 MLVNYY30 pKa = 10.23 SEE32 pKa = 4.6 EE33 pKa = 4.12 FDD35 pKa = 5.3 DD36 pKa = 5.77 ISPALLAKK44 pKa = 10.25 SFQKK48 pKa = 9.71 NAEE51 pKa = 4.23 MISEE55 pKa = 4.04 YY56 pKa = 10.38 RR57 pKa = 11.84 VLSSAGEE64 pKa = 4.39 GIDD67 pKa = 3.7 YY68 pKa = 10.26 QGKK71 pKa = 9.06 VLLNSRR77 pKa = 11.84 AVRR80 pKa = 11.84 LLSYY84 pKa = 10.75 VEE86 pKa = 5.09 DD87 pKa = 3.48 MSGDD91 pKa = 3.5 EE92 pKa = 4.42 KK93 pKa = 11.44 VRR95 pKa = 11.84 TIQSKK100 pKa = 7.86 EE101 pKa = 3.44 LWLAEE106 pKa = 4.02 DD107 pKa = 3.22 MTFYY111 pKa = 11.0 VVSCMSTITMDD122 pKa = 3.5 KK123 pKa = 11.1 EE124 pKa = 3.65 EE125 pKa = 4.92 AICLNEE131 pKa = 3.85 HH132 pKa = 6.46 RR133 pKa = 11.84 SVVTTVEE140 pKa = 4.18 CEE142 pKa = 3.67 DD143 pKa = 5.83 DD144 pKa = 3.43 IFFDD148 pKa = 3.85 MGSLICEE155 pKa = 4.64 LDD157 pKa = 4.38 DD158 pKa = 3.63 ICLFEE163 pKa = 5.52 LLADD167 pKa = 4.03 VDD169 pKa = 3.72 ATIYY173 pKa = 10.61 EE174 pKa = 4.33 LL175 pKa = 4.55
Molecular weight: 19.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.884
IPC_protein 3.859
Toseland 3.656
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.694
Grimsley 3.567
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.139
Thurlkill 3.694
EMBOSS 3.757
Sillero 3.973
Patrickios 1.125
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.875
Protein with the highest isoelectric point:
>tr|D4JZH8|D4JZH8_9FIRM K+ transport systems NAD-binding component OS=Faecalibacterium prausnitzii L2-6 OX=718252 GN=FP2_20320 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPKK8 pKa = 8.47 KK9 pKa = 7.63 RR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 7.09 EE14 pKa = 3.35 VHH16 pKa = 5.99 GFLTRR21 pKa = 11.84 MSTKK25 pKa = 10.35 NGRR28 pKa = 11.84 KK29 pKa = 9.33 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.76 GRR39 pKa = 11.84 KK40 pKa = 8.97 SLTVV44 pKa = 3.12
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 10.774
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.34
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.076
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.036
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2755
0
2755
789465
14
1989
286.6
31.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.938 ± 0.064
1.718 ± 0.019
5.701 ± 0.037
6.766 ± 0.047
3.796 ± 0.034
7.507 ± 0.046
1.866 ± 0.022
5.59 ± 0.042
5.472 ± 0.045
9.677 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.882 ± 0.027
3.558 ± 0.032
4.047 ± 0.033
3.482 ± 0.028
5.204 ± 0.041
5.551 ± 0.038
5.587 ± 0.034
7.04 ± 0.038
1.033 ± 0.018
3.587 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here