Bacillus infantis NRRL B-14911

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus infantis

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5066 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5L9X9|U5L9X9_9BACI Sulfate adenylyltransferase OS=Bacillus infantis NRRL B-14911 OX=1367477 GN=sat PE=3 SV=1
MM1 pKa = 7.54KK2 pKa = 9.74PHH4 pKa = 7.07KK5 pKa = 10.24RR6 pKa = 11.84PQLTPNNVQEE16 pKa = 3.94EE17 pKa = 4.97CIRR20 pKa = 11.84VSKK23 pKa = 10.81VYY25 pKa = 10.63DD26 pKa = 3.37WVFDD30 pKa = 5.17AITTDD35 pKa = 3.23TGITLPPDD43 pKa = 3.59CEE45 pKa = 4.02AAVALAVAEE54 pKa = 4.26GRR56 pKa = 11.84TPLDD60 pKa = 3.32VTCCVPDD67 pKa = 3.27VGGFFPLDD75 pKa = 3.78PPDD78 pKa = 3.51TDD80 pKa = 4.64GNATCTVSSRR90 pKa = 11.84IEE92 pKa = 3.72RR93 pKa = 11.84RR94 pKa = 11.84KK95 pKa = 9.93IPVNGVMTDD104 pKa = 3.44LAIVKK109 pKa = 9.07VIFTIRR115 pKa = 11.84PLVTIYY121 pKa = 10.94DD122 pKa = 3.61STGAVICSFRR132 pKa = 11.84PTISEE137 pKa = 3.72SRR139 pKa = 11.84RR140 pKa = 11.84LVVCAPEE147 pKa = 4.86PFTSDD152 pKa = 3.05NVFCRR157 pKa = 11.84LISLNCEE164 pKa = 3.81TNFIEE169 pKa = 5.04TGIPDD174 pKa = 3.52VGLQIMLDD182 pKa = 3.2ICFEE186 pKa = 4.14IQVEE190 pKa = 4.17ADD192 pKa = 3.29VKK194 pKa = 11.4LEE196 pKa = 3.91VLAKK200 pKa = 10.42FCFPRR205 pKa = 11.84PNDD208 pKa = 3.25IVIPPGGVCPPFEE221 pKa = 4.38WPEE224 pKa = 3.72QCDD227 pKa = 3.51FFPRR231 pKa = 11.84DD232 pKa = 3.42NCDD235 pKa = 3.04CQAFVDD241 pKa = 4.24TDD243 pKa = 5.0PITGPVPIVFSPILFAEE260 pKa = 4.6DD261 pKa = 3.64LAAGTYY267 pKa = 6.09TTEE270 pKa = 4.35LRR272 pKa = 11.84AEE274 pKa = 4.1ICDD277 pKa = 3.52NCQLTGSTLQWIVEE291 pKa = 4.3DD292 pKa = 4.24FVVPTGTGTLAVDD305 pKa = 3.1QSFVFTAEE313 pKa = 3.98EE314 pKa = 4.52FNMPTCSTVLGITTLTVTGAGTIVFSDD341 pKa = 3.75PSVTDD346 pKa = 3.46RR347 pKa = 11.84TALFTLTLVEE357 pKa = 5.35NIGTDD362 pKa = 3.36LDD364 pKa = 4.19AYY366 pKa = 10.98SITLTDD372 pKa = 3.06IAGAPLVTFAGAGTGFVPDD391 pKa = 4.38EE392 pKa = 4.31DD393 pKa = 6.38LIIQDD398 pKa = 4.37CVTFPNLLGGQPLL411 pKa = 3.4

Molecular weight: 44.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5LGP7|U5LGP7_9BACI Succinate dehydrogenase OS=Bacillus infantis NRRL B-14911 OX=1367477 GN=N288_19350 PE=3 SV=1
MM1 pKa = 8.07RR2 pKa = 11.84YY3 pKa = 9.34ILLLLIVIPAAEE15 pKa = 4.24IGVLLLSGKK24 pKa = 10.46VIGVWLTILLIICTGLLGAYY44 pKa = 8.54LAKK47 pKa = 10.48KK48 pKa = 10.03QGLEE52 pKa = 3.94TIRR55 pKa = 11.84RR56 pKa = 11.84VQEE59 pKa = 3.55QLGRR63 pKa = 11.84GQLPGDD69 pKa = 3.63AVLDD73 pKa = 4.81GICILVGGTLLLTPGFITDD92 pKa = 3.06ITGFLLLLPPSRR104 pKa = 11.84IFFKK108 pKa = 10.89NILMKK113 pKa = 10.26IFRR116 pKa = 11.84SRR118 pKa = 11.84IQRR121 pKa = 11.84GNIKK125 pKa = 10.17IIRR128 pKa = 3.95

Molecular weight: 14.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5066

0

5066

1383718

26

3826

273.1

30.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.073 ± 0.046

0.708 ± 0.01

5.057 ± 0.031

7.915 ± 0.052

4.614 ± 0.032

7.46 ± 0.035

1.953 ± 0.017

7.394 ± 0.032

6.647 ± 0.031

9.979 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.838 ± 0.018

3.802 ± 0.023

3.746 ± 0.02

3.459 ± 0.026

4.326 ± 0.031

6.357 ± 0.03

4.807 ± 0.024

6.367 ± 0.031

1.047 ± 0.016

3.452 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski