Adlercreutzia equolifaciens subsp. celatus
Average proteome isoelectric point is 5.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2195 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A369P163|A0A369P163_9ACTN Energy-dependent translational throttle protein EttA OS=Adlercreutzia equolifaciens subsp. celatus OX=394340 GN=ettA PE=3 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.19 ASKK5 pKa = 10.41 LIAAVVSGAAALALVGGIAYY25 pKa = 10.33 AMEE28 pKa = 5.52 PDD30 pKa = 4.49 HH31 pKa = 7.49 DD32 pKa = 4.58 PATLPMPEE40 pKa = 4.27 AAQDD44 pKa = 3.52 TSIVKK49 pKa = 9.41 QVASIIVYY57 pKa = 9.62 EE58 pKa = 4.25 DD59 pKa = 3.14 QEE61 pKa = 4.39 EE62 pKa = 4.29 AVAMASEE69 pKa = 4.48 FDD71 pKa = 4.93 DD72 pKa = 3.6 IHH74 pKa = 8.42 DD75 pKa = 4.42 EE76 pKa = 4.12 LAMDD80 pKa = 4.69 AVSSRR85 pKa = 11.84 ASLSAKK91 pKa = 10.05 HH92 pKa = 5.95 SASHH96 pKa = 7.44 DD97 pKa = 3.63 ATAKK101 pKa = 8.83 TAASAAGEE109 pKa = 4.31 TPEE112 pKa = 5.48 GSDD115 pKa = 4.54 DD116 pKa = 4.53 SIDD119 pKa = 3.67 VEE121 pKa = 4.25 AVDD124 pKa = 3.55 VTEE127 pKa = 4.21 PLEE130 pKa = 4.58 TISPEE135 pKa = 3.91 GDD137 pKa = 3.08 EE138 pKa = 4.45 MVADD142 pKa = 4.57 SIDD145 pKa = 3.57 VNGDD149 pKa = 2.44 IVTYY153 pKa = 9.8 VPSYY157 pKa = 10.65 EE158 pKa = 4.33 SASAPASGAGLWMGSDD174 pKa = 3.52 STTDD178 pKa = 3.49 GSWGYY183 pKa = 10.53 FIGHH187 pKa = 6.35 NPGDD191 pKa = 4.14 FTCVMTLKK199 pKa = 10.81 NGDD202 pKa = 3.95 PVTVCDD208 pKa = 3.71 SDD210 pKa = 4.65 RR211 pKa = 11.84 NTRR214 pKa = 11.84 TYY216 pKa = 11.25 SVVDD220 pKa = 3.56 EE221 pKa = 5.64 FIVPDD226 pKa = 3.44 DD227 pKa = 4.65 TYY229 pKa = 11.23 WEE231 pKa = 5.31 DD232 pKa = 3.12 IQSRR236 pKa = 11.84 VTDD239 pKa = 3.7 YY240 pKa = 11.52 GEE242 pKa = 4.65 SIILQTCCGDD252 pKa = 3.33 NAHH255 pKa = 6.06 YY256 pKa = 10.03 RR257 pKa = 11.84 VVIAAA262 pKa = 4.3
Molecular weight: 27.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.592
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.63
Grimsley 3.49
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.935
Patrickios 1.227
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.81
Protein with the highest isoelectric point:
>tr|A0A369P1C8|A0A369P1C8_9ACTN Peptidase OS=Adlercreutzia equolifaciens subsp. celatus OX=394340 GN=C1850_03650 PE=4 SV=1
MM1 pKa = 7.25 NVGSIDD7 pKa = 4.8 LIPRR11 pKa = 11.84 TLFHH15 pKa = 6.66 GRR17 pKa = 11.84 GTISCLPMNVYY28 pKa = 10.56 LSHH31 pKa = 6.01 NTALLFWRR39 pKa = 11.84 AWSAAAAIPLCVFHH53 pKa = 7.53 DD54 pKa = 4.45 LGVADD59 pKa = 5.17 ASLFPATLFPTSEE72 pKa = 4.07 VLRR75 pKa = 11.84 NEE77 pKa = 4.07 AVLSRR82 pKa = 11.84 DD83 pKa = 2.76 VRR85 pKa = 11.84 TILEE89 pKa = 4.51 DD90 pKa = 3.58 ALQSLAGAGAARR102 pKa = 11.84 LADD105 pKa = 4.27 AGSVASPDD113 pKa = 3.4 GSAAPAAAVGGMLPQTTAVASALKK137 pKa = 10.15 EE138 pKa = 4.16 VVRR141 pKa = 11.84 SRR143 pKa = 11.84 GAAPLHH149 pKa = 5.9 IMVAKK154 pKa = 10.39 GHH156 pKa = 5.13 GTRR159 pKa = 11.84 NSSDD163 pKa = 4.27 LIRR166 pKa = 11.84 HH167 pKa = 6.16 RR168 pKa = 11.84 SVAAFPRR175 pKa = 11.84 QAFIKK180 pKa = 8.81 VAPGVFVCSPEE191 pKa = 4.04 LVFVQMVSSLTRR203 pKa = 11.84 GGLLAVGYY211 pKa = 6.94 EE212 pKa = 4.11 LCGCYY217 pKa = 9.84 PLGEE221 pKa = 4.29 EE222 pKa = 4.35 RR223 pKa = 11.84 VGALVRR229 pKa = 11.84 RR230 pKa = 11.84 PLSSPRR236 pKa = 11.84 RR237 pKa = 11.84 LTAFAARR244 pKa = 11.84 AKK246 pKa = 10.24 GLAGVKK252 pKa = 8.76 VARR255 pKa = 11.84 SAAKK259 pKa = 9.69 QVLAKK264 pKa = 10.25 SGSLMEE270 pKa = 4.4 TEE272 pKa = 4.19 VSIIAFSLFSQGGLGLRR289 pKa = 11.84 TPLLNEE295 pKa = 3.73 PVALSDD301 pKa = 3.81 RR302 pKa = 11.84 ARR304 pKa = 11.84 TATGLSRR311 pKa = 11.84 VVCDD315 pKa = 3.66 WLWPAEE321 pKa = 4.09 RR322 pKa = 11.84 VVLEE326 pKa = 4.04 YY327 pKa = 10.65 DD328 pKa = 3.08 GHH330 pKa = 6.87 DD331 pKa = 3.37 AHH333 pKa = 7.61 SSPQQRR339 pKa = 11.84 AHH341 pKa = 7.44 DD342 pKa = 3.78 ARR344 pKa = 11.84 KK345 pKa = 9.72 RR346 pKa = 11.84 DD347 pKa = 3.54 ALRR350 pKa = 11.84 IDD352 pKa = 4.06 GFDD355 pKa = 3.66 LAVLTSSQFHH365 pKa = 6.71 HH366 pKa = 7.0 VNQCTEE372 pKa = 4.04 LLRR375 pKa = 11.84 GVARR379 pKa = 11.84 SAGQRR384 pKa = 11.84 SPALKK389 pKa = 10.41 LEE391 pKa = 4.05 HH392 pKa = 6.69 LPRR395 pKa = 11.84 HH396 pKa = 5.2 LALRR400 pKa = 11.84 EE401 pKa = 3.9 QVRR404 pKa = 11.84 KK405 pKa = 8.89 HH406 pKa = 5.63 HH407 pKa = 6.41 RR408 pKa = 11.84 AHH410 pKa = 6.15 FPSRR414 pKa = 11.84 FRR416 pKa = 11.84 RR417 pKa = 11.84 ARR419 pKa = 11.84 SS420 pKa = 3.13
Molecular weight: 45.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.399
IPC_protein 10.218
Toseland 10.599
ProMoST 10.335
Dawson 10.672
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.745
Grimsley 10.716
Solomon 10.833
Lehninger 10.804
Nozaki 10.613
DTASelect 10.409
Thurlkill 10.599
EMBOSS 11.008
Sillero 10.628
Patrickios 10.452
IPC_peptide 10.847
IPC2_peptide 9.706
IPC2.peptide.svr19 8.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2195
0
2195
753156
37
4028
343.1
37.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.51 ± 0.079
1.599 ± 0.025
6.028 ± 0.053
6.899 ± 0.053
3.8 ± 0.037
8.278 ± 0.05
1.743 ± 0.022
4.917 ± 0.044
3.485 ± 0.04
9.326 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.647 ± 0.025
2.831 ± 0.033
4.528 ± 0.034
2.683 ± 0.023
5.96 ± 0.054
5.467 ± 0.045
5.32 ± 0.043
7.996 ± 0.041
1.16 ± 0.02
2.823 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here