Jonquetella anthropi DSM 22815
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1508 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H0UJV2|H0UJV2_9BACT N6-adenine-specific methylase OS=Jonquetella anthropi DSM 22815 OX=885272 GN=JonanDRAFT_0558 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.22 KK3 pKa = 10.16 YY4 pKa = 10.62 VCTVCGFVYY13 pKa = 10.33 DD14 pKa = 4.61 PEE16 pKa = 5.31 VGDD19 pKa = 4.46 PDD21 pKa = 4.6 SGIAPGTAFEE31 pKa = 5.97 DD32 pKa = 4.28 IPDD35 pKa = 3.65 DD36 pKa = 4.04 WVCPEE41 pKa = 4.51 CGVGKK46 pKa = 10.48 DD47 pKa = 3.4 MFEE50 pKa = 4.18 EE51 pKa = 4.25 QQ52 pKa = 3.95
Molecular weight: 5.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.828
IPC2_protein 3.706
IPC_protein 3.605
Toseland 3.414
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.592
Rodwell 3.452
Grimsley 3.338
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 3.948
Thurlkill 3.503
EMBOSS 3.592
Sillero 3.745
Patrickios 0.299
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|H0UK18|H0UK18_9BACT Indolepyruvate oxidoreductase subunit IorA OS=Jonquetella anthropi DSM 22815 OX=885272 GN=JonanDRAFT_0633 PE=4 SV=1
MM1 pKa = 7.26 KK2 pKa = 9.53 QTFQPHH8 pKa = 4.55 NRR10 pKa = 11.84 PRR12 pKa = 11.84 KK13 pKa = 8.75 RR14 pKa = 11.84 SMGFLARR21 pKa = 11.84 SRR23 pKa = 11.84 SVGGRR28 pKa = 11.84 AVLANRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.81 GRR39 pKa = 11.84 KK40 pKa = 8.69 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.618
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.34
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1508
0
1508
500423
36
3570
331.8
36.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.497 ± 0.077
1.477 ± 0.022
5.344 ± 0.048
6.022 ± 0.064
3.8 ± 0.04
8.58 ± 0.064
1.624 ± 0.025
4.82 ± 0.052
4.503 ± 0.052
10.735 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.551 ± 0.03
2.692 ± 0.041
4.728 ± 0.04
3.221 ± 0.038
6.363 ± 0.06
6.433 ± 0.045
4.984 ± 0.046
7.836 ± 0.063
1.408 ± 0.028
2.386 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here