Escherichia coli O157 typing phage 10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Uetakevirus; unclassified Uetakevirus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6TK62|A0A0F6TK62_9CAUD Uncharacterized protein OS=Escherichia coli O157 typing phage 10 OX=1508672 GN=ECTP10_01659 PE=4 SV=1
MM1 pKa = 7.51TNRR4 pKa = 11.84EE5 pKa = 4.2LVDD8 pKa = 3.54AAIKK12 pKa = 10.44LAGDD16 pKa = 4.88FYY18 pKa = 11.88SMMGYY23 pKa = 10.1AHH25 pKa = 7.55RR26 pKa = 11.84PGFKK30 pKa = 9.71YY31 pKa = 9.75WEE33 pKa = 4.47SPHH36 pKa = 6.21PQEE39 pKa = 4.63QLVFQMACRR48 pKa = 11.84AFEE51 pKa = 4.38VIRR54 pKa = 11.84GSDD57 pKa = 3.48VMDD60 pKa = 4.49AVADD64 pKa = 4.17LEE66 pKa = 4.75DD67 pKa = 3.97EE68 pKa = 4.4EE69 pKa = 4.78

Molecular weight: 7.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6R8N5|A0A0F6R8N5_9CAUD Uncharacterized protein OS=Escherichia coli O157 typing phage 10 OX=1508672 GN=ECTP10_01661 PE=4 SV=1
MM1 pKa = 7.39LRR3 pKa = 11.84GDD5 pKa = 4.45GAQLLQKK12 pKa = 9.66AQRR15 pKa = 11.84VTEE18 pKa = 3.98QAEE21 pKa = 3.95EE22 pKa = 4.04RR23 pKa = 11.84VRR25 pKa = 11.84SKK27 pKa = 10.37MKK29 pKa = 10.07NRR31 pKa = 11.84KK32 pKa = 9.11AKK34 pKa = 8.8MLISRR39 pKa = 11.84VYY41 pKa = 9.23RR42 pKa = 11.84RR43 pKa = 11.84CYY45 pKa = 8.07PSQWLRR51 pKa = 11.84VSNRR55 pKa = 11.84RR56 pKa = 11.84VVLYY60 pKa = 9.64LYY62 pKa = 10.71SGIARR67 pKa = 11.84EE68 pKa = 4.36GIKK71 pKa = 10.35DD72 pKa = 3.56KK73 pKa = 11.16RR74 pKa = 11.84SAAQNRR80 pKa = 11.84WKK82 pKa = 10.79NHH84 pKa = 4.22LRR86 pKa = 11.84AKK88 pKa = 10.63GDD90 pKa = 3.32

Molecular weight: 10.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

11836

46

875

236.7

26.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.181 ± 0.543

0.929 ± 0.14

5.804 ± 0.215

6.075 ± 0.35

3.312 ± 0.204

7.494 ± 0.404

1.495 ± 0.145

5.72 ± 0.305

5.044 ± 0.34

7.917 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.966 ± 0.291

4.664 ± 0.228

4.047 ± 0.224

4.858 ± 0.389

6.193 ± 0.321

6.446 ± 0.344

5.602 ± 0.359

6.413 ± 0.265

1.673 ± 0.174

3.168 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski