Amino acid dipepetide frequency for Escherichia coli O157 typing phage 10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.505AlaAla: 12.505 ± 1.412
0.845AlaCys: 0.845 ± 0.274
5.746AlaAsp: 5.746 ± 0.745
6.929AlaGlu: 6.929 ± 1.132
3.802AlaPhe: 3.802 ± 0.511
7.689AlaGly: 7.689 ± 0.842
1.267AlaHis: 1.267 ± 0.351
4.225AlaIle: 4.225 ± 0.643
4.816AlaLys: 4.816 ± 0.611
8.196AlaLeu: 8.196 ± 0.844
3.802AlaMet: 3.802 ± 0.535
4.647AlaAsn: 4.647 ± 0.495
3.295AlaPro: 3.295 ± 0.617
5.239AlaGln: 5.239 ± 0.841
5.577AlaArg: 5.577 ± 0.726
5.999AlaSer: 5.999 ± 0.557
5.154AlaThr: 5.154 ± 0.607
7.182AlaVal: 7.182 ± 0.854
1.605AlaTrp: 1.605 ± 0.387
3.295AlaTyr: 3.295 ± 0.614
0.0AlaXaa: 0.0 ± 0.0
Cys
0.676CysAla: 0.676 ± 0.226
0.084CysCys: 0.084 ± 0.086
0.591CysAsp: 0.591 ± 0.237
0.591CysGlu: 0.591 ± 0.169
0.507CysPhe: 0.507 ± 0.197
1.183CysGly: 1.183 ± 0.341
0.084CysHis: 0.084 ± 0.082
0.676CysIle: 0.676 ± 0.188
0.422CysLys: 0.422 ± 0.232
0.676CysLeu: 0.676 ± 0.252
0.422CysMet: 0.422 ± 0.215
0.338CysAsn: 0.338 ± 0.166
0.422CysPro: 0.422 ± 0.152
0.253CysGln: 0.253 ± 0.153
1.014CysArg: 1.014 ± 0.301
0.422CysSer: 0.422 ± 0.196
0.507CysThr: 0.507 ± 0.223
0.253CysVal: 0.253 ± 0.149
0.084CysTrp: 0.084 ± 0.094
0.084CysTyr: 0.084 ± 0.081
0.0CysXaa: 0.0 ± 0.0
Asp
6.168AspAla: 6.168 ± 0.817
0.845AspCys: 0.845 ± 0.291
4.14AspAsp: 4.14 ± 0.548
3.633AspGlu: 3.633 ± 0.684
1.436AspPhe: 1.436 ± 0.337
4.732AspGly: 4.732 ± 0.671
0.422AspHis: 0.422 ± 0.218
3.718AspIle: 3.718 ± 0.396
4.056AspLys: 4.056 ± 0.674
4.309AspLeu: 4.309 ± 0.558
1.69AspMet: 1.69 ± 0.341
2.45AspAsn: 2.45 ± 0.381
2.45AspPro: 2.45 ± 0.46
2.112AspGln: 2.112 ± 0.357
2.619AspArg: 2.619 ± 0.654
3.042AspSer: 3.042 ± 0.411
2.873AspThr: 2.873 ± 0.455
4.563AspVal: 4.563 ± 0.618
1.014AspTrp: 1.014 ± 0.309
1.774AspTyr: 1.774 ± 0.325
0.0AspXaa: 0.0 ± 0.0
Glu
6.337GluAla: 6.337 ± 0.775
0.845GluCys: 0.845 ± 0.267
3.126GluAsp: 3.126 ± 0.536
3.042GluGlu: 3.042 ± 0.605
3.042GluPhe: 3.042 ± 0.484
3.295GluGly: 3.295 ± 0.538
0.76GluHis: 0.76 ± 0.237
3.718GluIle: 3.718 ± 0.674
3.38GluLys: 3.38 ± 0.587
4.901GluLeu: 4.901 ± 0.688
1.436GluMet: 1.436 ± 0.337
1.943GluAsn: 1.943 ± 0.326
2.112GluPro: 2.112 ± 0.321
4.563GluGln: 4.563 ± 0.606
4.14GluArg: 4.14 ± 0.692
4.225GluSer: 4.225 ± 0.626
3.211GluThr: 3.211 ± 0.504
3.38GluVal: 3.38 ± 0.49
0.676GluTrp: 0.676 ± 0.308
2.535GluTyr: 2.535 ± 0.473
0.0GluXaa: 0.0 ± 0.0
Phe
2.873PheAla: 2.873 ± 0.466
0.253PheCys: 0.253 ± 0.14
2.197PheAsp: 2.197 ± 0.434
1.943PheGlu: 1.943 ± 0.35
1.267PhePhe: 1.267 ± 0.329
2.45PheGly: 2.45 ± 0.481
0.253PheHis: 0.253 ± 0.173
2.704PheIle: 2.704 ± 0.649
1.436PheLys: 1.436 ± 0.397
1.521PheLeu: 1.521 ± 0.444
1.014PheMet: 1.014 ± 0.253
1.69PheAsn: 1.69 ± 0.376
1.267PhePro: 1.267 ± 0.324
0.929PheGln: 0.929 ± 0.286
2.957PheArg: 2.957 ± 0.383
2.704PheSer: 2.704 ± 0.442
2.112PheThr: 2.112 ± 0.385
1.859PheVal: 1.859 ± 0.37
0.676PheTrp: 0.676 ± 0.193
1.014PheTyr: 1.014 ± 0.34
0.0PheXaa: 0.0 ± 0.0
Gly
5.661GlyAla: 5.661 ± 1.09
0.591GlyCys: 0.591 ± 0.2
4.394GlyAsp: 4.394 ± 0.61
4.901GlyGlu: 4.901 ± 0.793
3.126GlyPhe: 3.126 ± 0.493
5.83GlyGly: 5.83 ± 0.823
1.436GlyHis: 1.436 ± 0.35
4.732GlyIle: 4.732 ± 0.584
4.901GlyLys: 4.901 ± 0.7
6.168GlyLeu: 6.168 ± 0.8
2.197GlyMet: 2.197 ± 0.373
3.887GlyAsn: 3.887 ± 0.657
1.605GlyPro: 1.605 ± 0.306
3.802GlyGln: 3.802 ± 0.557
3.549GlyArg: 3.549 ± 0.588
3.887GlySer: 3.887 ± 0.699
4.394GlyThr: 4.394 ± 0.553
5.999GlyVal: 5.999 ± 0.811
1.014GlyTrp: 1.014 ± 0.301
2.873GlyTyr: 2.873 ± 0.342
0.0GlyXaa: 0.0 ± 0.0
His
0.845HisAla: 0.845 ± 0.279
0.422HisCys: 0.422 ± 0.219
0.76HisAsp: 0.76 ± 0.262
1.267HisGlu: 1.267 ± 0.34
0.422HisPhe: 0.422 ± 0.198
1.267HisGly: 1.267 ± 0.285
0.422HisHis: 0.422 ± 0.181
0.845HisIle: 0.845 ± 0.215
0.169HisLys: 0.169 ± 0.105
1.352HisLeu: 1.352 ± 0.386
0.422HisMet: 0.422 ± 0.166
0.676HisAsn: 0.676 ± 0.289
1.098HisPro: 1.098 ± 0.3
0.338HisGln: 0.338 ± 0.145
0.845HisArg: 0.845 ± 0.295
1.183HisSer: 1.183 ± 0.286
0.845HisThr: 0.845 ± 0.235
1.014HisVal: 1.014 ± 0.265
0.422HisTrp: 0.422 ± 0.166
0.338HisTyr: 0.338 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
6.506IleAla: 6.506 ± 0.822
0.422IleCys: 0.422 ± 0.221
4.14IleAsp: 4.14 ± 0.661
3.802IleGlu: 3.802 ± 0.585
1.352IlePhe: 1.352 ± 0.259
4.225IleGly: 4.225 ± 0.483
0.676IleHis: 0.676 ± 0.182
3.464IleIle: 3.464 ± 0.621
3.211IleLys: 3.211 ± 0.531
2.957IleLeu: 2.957 ± 0.623
1.521IleMet: 1.521 ± 0.478
3.549IleAsn: 3.549 ± 0.487
2.704IlePro: 2.704 ± 0.363
2.197IleGln: 2.197 ± 0.385
3.549IleArg: 3.549 ± 0.435
3.971IleSer: 3.971 ± 0.607
3.971IleThr: 3.971 ± 0.601
2.704IleVal: 2.704 ± 0.572
0.929IleTrp: 0.929 ± 0.244
1.352IleTyr: 1.352 ± 0.263
0.0IleXaa: 0.0 ± 0.0
Lys
4.647LysAla: 4.647 ± 0.674
0.507LysCys: 0.507 ± 0.235
2.197LysAsp: 2.197 ± 0.46
2.619LysGlu: 2.619 ± 0.537
1.436LysPhe: 1.436 ± 0.366
3.549LysGly: 3.549 ± 0.699
0.845LysHis: 0.845 ± 0.223
2.619LysIle: 2.619 ± 0.505
2.281LysLys: 2.281 ± 0.431
4.309LysLeu: 4.309 ± 0.658
1.267LysMet: 1.267 ± 0.323
2.619LysAsn: 2.619 ± 0.491
2.873LysPro: 2.873 ± 0.553
2.619LysGln: 2.619 ± 0.519
3.718LysArg: 3.718 ± 0.92
2.873LysSer: 2.873 ± 0.44
3.38LysThr: 3.38 ± 0.558
2.957LysVal: 2.957 ± 0.416
1.436LysTrp: 1.436 ± 0.324
1.69LysTyr: 1.69 ± 0.334
0.0LysXaa: 0.0 ± 0.0
Leu
8.365LeuAla: 8.365 ± 0.56
0.591LeuCys: 0.591 ± 0.22
4.478LeuAsp: 4.478 ± 0.438
4.647LeuGlu: 4.647 ± 0.612
2.957LeuPhe: 2.957 ± 0.671
5.07LeuGly: 5.07 ± 0.947
1.521LeuHis: 1.521 ± 0.333
4.563LeuIle: 4.563 ± 0.822
3.549LeuLys: 3.549 ± 0.523
6.422LeuLeu: 6.422 ± 0.845
2.197LeuMet: 2.197 ± 0.423
4.309LeuAsn: 4.309 ± 0.611
2.281LeuPro: 2.281 ± 0.376
3.211LeuGln: 3.211 ± 0.471
5.408LeuArg: 5.408 ± 0.743
6.422LeuSer: 6.422 ± 0.635
4.985LeuThr: 4.985 ± 0.634
4.647LeuVal: 4.647 ± 0.515
0.845LeuTrp: 0.845 ± 0.223
2.704LeuTyr: 2.704 ± 0.387
0.0LeuXaa: 0.0 ± 0.0
Met
4.056MetAla: 4.056 ± 0.815
0.253MetCys: 0.253 ± 0.154
1.183MetAsp: 1.183 ± 0.25
1.521MetGlu: 1.521 ± 0.364
0.929MetPhe: 0.929 ± 0.293
1.859MetGly: 1.859 ± 0.397
0.253MetHis: 0.253 ± 0.144
1.521MetIle: 1.521 ± 0.336
1.605MetLys: 1.605 ± 0.543
3.126MetLeu: 3.126 ± 0.586
1.014MetMet: 1.014 ± 0.407
1.774MetAsn: 1.774 ± 0.382
1.183MetPro: 1.183 ± 0.305
0.591MetGln: 0.591 ± 0.151
2.028MetArg: 2.028 ± 0.561
2.619MetSer: 2.619 ± 0.548
1.774MetThr: 1.774 ± 0.419
1.267MetVal: 1.267 ± 0.266
0.253MetTrp: 0.253 ± 0.135
0.845MetTyr: 0.845 ± 0.28
0.0MetXaa: 0.0 ± 0.0
Asn
4.394AsnAla: 4.394 ± 0.753
0.338AsnCys: 0.338 ± 0.173
2.704AsnAsp: 2.704 ± 0.383
2.619AsnGlu: 2.619 ± 0.431
1.267AsnPhe: 1.267 ± 0.253
4.225AsnGly: 4.225 ± 0.613
0.591AsnHis: 0.591 ± 0.203
2.619AsnIle: 2.619 ± 0.496
2.366AsnLys: 2.366 ± 0.455
3.126AsnLeu: 3.126 ± 0.652
1.352AsnMet: 1.352 ± 0.43
2.028AsnAsn: 2.028 ± 0.565
2.873AsnPro: 2.873 ± 0.483
2.704AsnGln: 2.704 ± 0.575
2.957AsnArg: 2.957 ± 0.467
3.211AsnSer: 3.211 ± 0.71
2.45AsnThr: 2.45 ± 0.43
2.535AsnVal: 2.535 ± 0.496
0.676AsnTrp: 0.676 ± 0.245
1.605AsnTyr: 1.605 ± 0.419
0.0AsnXaa: 0.0 ± 0.0
Pro
3.126ProAla: 3.126 ± 0.598
0.169ProCys: 0.169 ± 0.128
3.464ProAsp: 3.464 ± 0.613
3.126ProGlu: 3.126 ± 0.512
1.183ProPhe: 1.183 ± 0.271
4.056ProGly: 4.056 ± 0.483
1.014ProHis: 1.014 ± 0.267
1.69ProIle: 1.69 ± 0.345
1.605ProLys: 1.605 ± 0.385
2.366ProLeu: 2.366 ± 0.463
0.76ProMet: 0.76 ± 0.2
0.845ProAsn: 0.845 ± 0.24
1.69ProPro: 1.69 ± 0.504
2.281ProGln: 2.281 ± 0.389
1.521ProArg: 1.521 ± 0.3
3.126ProSer: 3.126 ± 0.633
1.943ProThr: 1.943 ± 0.4
4.309ProVal: 4.309 ± 0.483
0.929ProTrp: 0.929 ± 0.301
1.267ProTyr: 1.267 ± 0.31
0.0ProXaa: 0.0 ± 0.0
Gln
6.253GlnAla: 6.253 ± 1.109
0.591GlnCys: 0.591 ± 0.242
1.69GlnAsp: 1.69 ± 0.313
3.211GlnGlu: 3.211 ± 0.391
1.521GlnPhe: 1.521 ± 0.373
2.366GlnGly: 2.366 ± 0.367
0.676GlnHis: 0.676 ± 0.259
2.281GlnIle: 2.281 ± 0.486
2.028GlnLys: 2.028 ± 0.337
5.239GlnLeu: 5.239 ± 0.732
2.028GlnMet: 2.028 ± 0.412
1.521GlnAsn: 1.521 ± 0.326
1.69GlnPro: 1.69 ± 0.385
3.887GlnGln: 3.887 ± 0.756
3.633GlnArg: 3.633 ± 0.594
2.028GlnSer: 2.028 ± 0.524
1.943GlnThr: 1.943 ± 0.411
3.042GlnVal: 3.042 ± 0.661
1.183GlnTrp: 1.183 ± 0.378
1.774GlnTyr: 1.774 ± 0.384
0.0GlnXaa: 0.0 ± 0.0
Arg
6.168ArgAla: 6.168 ± 0.838
0.507ArgCys: 0.507 ± 0.186
3.887ArgAsp: 3.887 ± 0.552
4.056ArgGlu: 4.056 ± 0.543
1.605ArgPhe: 1.605 ± 0.282
3.126ArgGly: 3.126 ± 0.536
0.929ArgHis: 0.929 ± 0.222
4.732ArgIle: 4.732 ± 0.575
4.14ArgLys: 4.14 ± 0.554
4.985ArgLeu: 4.985 ± 0.591
1.098ArgMet: 1.098 ± 0.291
3.042ArgAsn: 3.042 ± 0.399
1.267ArgPro: 1.267 ± 0.256
4.14ArgGln: 4.14 ± 0.582
4.985ArgArg: 4.985 ± 0.643
2.873ArgSer: 2.873 ± 0.506
3.126ArgThr: 3.126 ± 0.51
4.225ArgVal: 4.225 ± 0.969
1.521ArgTrp: 1.521 ± 0.384
1.521ArgTyr: 1.521 ± 0.365
0.0ArgXaa: 0.0 ± 0.0
Ser
5.83SerAla: 5.83 ± 0.689
0.507SerCys: 0.507 ± 0.191
4.14SerAsp: 4.14 ± 0.556
3.971SerGlu: 3.971 ± 0.524
2.281SerPhe: 2.281 ± 0.431
5.915SerGly: 5.915 ± 1.019
0.929SerHis: 0.929 ± 0.242
2.873SerIle: 2.873 ± 0.406
3.126SerLys: 3.126 ± 0.532
5.577SerLeu: 5.577 ± 0.696
1.943SerMet: 1.943 ± 0.44
3.126SerAsn: 3.126 ± 0.537
3.38SerPro: 3.38 ± 0.444
2.788SerGln: 2.788 ± 0.558
3.887SerArg: 3.887 ± 0.684
3.633SerSer: 3.633 ± 0.714
3.802SerThr: 3.802 ± 0.542
3.971SerVal: 3.971 ± 0.714
0.845SerTrp: 0.845 ± 0.259
1.943SerTyr: 1.943 ± 0.368
0.0SerXaa: 0.0 ± 0.0
Thr
6.929ThrAla: 6.929 ± 0.898
0.422ThrCys: 0.422 ± 0.197
3.38ThrAsp: 3.38 ± 0.716
2.366ThrGlu: 2.366 ± 0.444
1.436ThrPhe: 1.436 ± 0.286
7.351ThrGly: 7.351 ± 0.943
0.676ThrHis: 0.676 ± 0.229
3.042ThrIle: 3.042 ± 0.492
2.197ThrLys: 2.197 ± 0.421
4.394ThrLeu: 4.394 ± 0.665
1.774ThrMet: 1.774 ± 0.362
2.281ThrAsn: 2.281 ± 0.37
3.38ThrPro: 3.38 ± 0.377
2.028ThrGln: 2.028 ± 0.516
2.704ThrArg: 2.704 ± 0.502
3.971ThrSer: 3.971 ± 0.798
2.535ThrThr: 2.535 ± 0.381
3.126ThrVal: 3.126 ± 0.602
0.845ThrTrp: 0.845 ± 0.302
1.183ThrTyr: 1.183 ± 0.358
0.0ThrXaa: 0.0 ± 0.0
Val
5.999ValAla: 5.999 ± 0.667
0.507ValCys: 0.507 ± 0.173
3.718ValAsp: 3.718 ± 0.64
3.295ValGlu: 3.295 ± 0.37
1.859ValPhe: 1.859 ± 0.345
2.873ValGly: 2.873 ± 0.59
1.098ValHis: 1.098 ± 0.279
4.225ValIle: 4.225 ± 0.702
3.211ValLys: 3.211 ± 0.541
4.901ValLeu: 4.901 ± 0.535
2.619ValMet: 2.619 ± 0.45
4.225ValAsn: 4.225 ± 0.63
2.535ValPro: 2.535 ± 0.52
2.535ValGln: 2.535 ± 0.391
3.633ValArg: 3.633 ± 0.359
4.732ValSer: 4.732 ± 0.568
4.225ValThr: 4.225 ± 0.5
4.732ValVal: 4.732 ± 0.725
0.845ValTrp: 0.845 ± 0.197
2.366ValTyr: 2.366 ± 0.421
0.0ValXaa: 0.0 ± 0.0
Trp
1.859TrpAla: 1.859 ± 0.409
0.169TrpCys: 0.169 ± 0.107
0.591TrpAsp: 0.591 ± 0.228
1.014TrpGlu: 1.014 ± 0.244
0.76TrpPhe: 0.76 ± 0.252
1.352TrpGly: 1.352 ± 0.39
0.169TrpHis: 0.169 ± 0.131
0.591TrpIle: 0.591 ± 0.24
0.845TrpLys: 0.845 ± 0.28
1.943TrpLeu: 1.943 ± 0.465
0.507TrpMet: 0.507 ± 0.218
0.845TrpAsn: 0.845 ± 0.248
0.422TrpPro: 0.422 ± 0.213
0.507TrpGln: 0.507 ± 0.207
1.098TrpArg: 1.098 ± 0.293
1.352TrpSer: 1.352 ± 0.308
0.76TrpThr: 0.76 ± 0.239
1.014TrpVal: 1.014 ± 0.328
0.338TrpTrp: 0.338 ± 0.161
0.591TrpTyr: 0.591 ± 0.188
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.619TyrAla: 2.619 ± 0.357
0.422TyrCys: 0.422 ± 0.219
1.521TyrAsp: 1.521 ± 0.37
2.197TyrGlu: 2.197 ± 0.377
0.929TyrPhe: 0.929 ± 0.291
2.535TyrGly: 2.535 ± 0.49
0.929TyrHis: 0.929 ± 0.329
2.366TyrIle: 2.366 ± 0.345
1.098TyrLys: 1.098 ± 0.272
2.704TyrLeu: 2.704 ± 0.576
0.507TyrMet: 0.507 ± 0.24
0.845TyrAsn: 0.845 ± 0.311
1.943TyrPro: 1.943 ± 0.447
1.859TyrGln: 1.859 ± 0.406
2.112TyrArg: 2.112 ± 0.437
2.197TyrSer: 2.197 ± 0.354
2.028TyrThr: 2.028 ± 0.473
1.352TyrVal: 1.352 ± 0.329
0.591TyrTrp: 0.591 ± 0.201
0.929TyrTyr: 0.929 ± 0.26
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (11836 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski