Fulmarus glacialis (Northern fulmar)
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8115 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A093KNT1|A0A093KNT1_FULGA Uncharacterized protein (Fragment) OS=Fulmarus glacialis OX=30455 GN=N327_02277 PE=4 SV=1
GG1 pKa = 7.59 SYY3 pKa = 10.4 HH4 pKa = 7.4 CGQCKK9 pKa = 9.56 PGYY12 pKa = 9.17 TGDD15 pKa = 3.61 QARR18 pKa = 11.84 GCQAEE23 pKa = 4.14 RR24 pKa = 11.84 SCRR27 pKa = 11.84 NRR29 pKa = 11.84 ALNPCSVHH37 pKa = 5.72 ARR39 pKa = 11.84 CIEE42 pKa = 3.78 EE43 pKa = 3.82 RR44 pKa = 11.84 RR45 pKa = 11.84 GEE47 pKa = 4.25 VTCICGIGWAGDD59 pKa = 3.89 GYY61 pKa = 11.59 VCGKK65 pKa = 10.32 DD66 pKa = 3.5 VDD68 pKa = 3.48 IDD70 pKa = 4.16 GYY72 pKa = 10.1 PNEE75 pKa = 4.55 EE76 pKa = 4.13 LSCSAEE82 pKa = 3.8 NCRR85 pKa = 11.84 KK86 pKa = 10.08 DD87 pKa = 3.11 NCRR90 pKa = 11.84 FVPNSGQEE98 pKa = 4.06 DD99 pKa = 4.09 ADD101 pKa = 3.68 SDD103 pKa = 4.74 GIGDD107 pKa = 4.44 ACDD110 pKa = 4.46 DD111 pKa = 4.56 DD112 pKa = 6.96 ADD114 pKa = 4.36 GDD116 pKa = 4.52 GIPNEE121 pKa = 4.01 QDD123 pKa = 3.11 NCVLAPNVNQRR134 pKa = 11.84 NGDD137 pKa = 3.22 QDD139 pKa = 3.49 IFGDD143 pKa = 3.76 ACDD146 pKa = 3.38 NCRR149 pKa = 11.84 NVLNNDD155 pKa = 3.07 QRR157 pKa = 11.84 DD158 pKa = 3.45 TDD160 pKa = 3.87 GDD162 pKa = 4.16 GKK164 pKa = 11.42 GDD166 pKa = 3.74 ACDD169 pKa = 5.17 DD170 pKa = 4.26 DD171 pKa = 4.15 MDD173 pKa = 6.26 GDD175 pKa = 4.5 GIKK178 pKa = 10.77 NLLDD182 pKa = 3.41 NCQRR186 pKa = 11.84 IPNQDD191 pKa = 3.43 QEE193 pKa = 5.62 DD194 pKa = 3.92 KK195 pKa = 11.46 DD196 pKa = 3.9 NDD198 pKa = 4.31 GVGDD202 pKa = 4.05 ACDD205 pKa = 3.47 SCPAISNPNQSDD217 pKa = 3.32 VDD219 pKa = 3.63 NDD221 pKa = 4.07 LVGDD225 pKa = 4.11 SCDD228 pKa = 3.63 TNQDD232 pKa = 3.04 SDD234 pKa = 4.07 GDD236 pKa = 4.03 GHH238 pKa = 7.17 QDD240 pKa = 3.22 STDD243 pKa = 3.33 NCPTIINSSQLDD255 pKa = 3.61 TDD257 pKa = 3.69 KK258 pKa = 11.62 DD259 pKa = 4.04 GLGDD263 pKa = 3.83 EE264 pKa = 5.5 CDD266 pKa = 5.74 DD267 pKa = 6.0 DD268 pKa = 7.44 DD269 pKa = 7.55 DD270 pKa = 7.26 DD271 pKa = 7.31 DD272 pKa = 6.25 GIPDD276 pKa = 5.2 LLPPGPDD283 pKa = 2.92 NCRR286 pKa = 11.84 LVPNPGQEE294 pKa = 4.19 DD295 pKa = 4.25 EE296 pKa = 4.92 NGDD299 pKa = 3.9 GVGDD303 pKa = 3.51 ICEE306 pKa = 4.29 SDD308 pKa = 3.45 FDD310 pKa = 3.9 QDD312 pKa = 3.89 TVIDD316 pKa = 4.23 QIDD319 pKa = 3.82 VCPEE323 pKa = 3.54 NAEE326 pKa = 3.78 ITLTDD331 pKa = 3.83 FRR333 pKa = 11.84 AYY335 pKa = 8.19 QTVVLDD341 pKa = 4.22 PEE343 pKa = 5.32 GDD345 pKa = 3.65 AQIDD349 pKa = 4.13 PNWVVLNQGMEE360 pKa = 4.11 IVQTMNSDD368 pKa = 3.19 PGLAVGYY375 pKa = 7.31 TAFNGVDD382 pKa = 3.99 FEE384 pKa = 4.97 GTFHH388 pKa = 6.81 VNTVTDD394 pKa = 3.57 DD395 pKa = 4.08 DD396 pKa = 4.35 YY397 pKa = 12.0 AGFIFGYY404 pKa = 9.58 QDD406 pKa = 2.83 SSSFYY411 pKa = 10.47 VVMWKK416 pKa = 7.78 QTEE419 pKa = 3.86 QTYY422 pKa = 8.13 WQATPFRR429 pKa = 11.84 AVAEE433 pKa = 4.17 PGIQLKK439 pKa = 10.07 AVKK442 pKa = 10.09 SKK444 pKa = 9.55 TGPGEE449 pKa = 3.82 HH450 pKa = 6.52 LRR452 pKa = 11.84 NSLWHH457 pKa = 6.51 TGDD460 pKa = 3.06 TSDD463 pKa = 5.19 QVRR466 pKa = 11.84 LLWKK470 pKa = 10.3 DD471 pKa = 3.22 PRR473 pKa = 11.84 NVGWKK478 pKa = 10.39 DD479 pKa = 3.18 KK480 pKa = 11.21 VSYY483 pKa = 10.33 RR484 pKa = 11.84 WFLQHH489 pKa = 6.9 RR490 pKa = 11.84 PQIGYY495 pKa = 9.07 IRR497 pKa = 11.84 ARR499 pKa = 11.84 FYY501 pKa = 11.09 EE502 pKa = 4.54 GSDD505 pKa = 3.39 LVADD509 pKa = 3.84 SGVTIDD515 pKa = 3.09 TTMRR519 pKa = 11.84 GGRR522 pKa = 11.84 LGVFCFSQEE531 pKa = 3.94 NIIWSNLKK539 pKa = 9.68 YY540 pKa = 10.31 RR541 pKa = 11.84 CNDD544 pKa = 4.19 TIPDD548 pKa = 4.06 DD549 pKa = 3.93 FQEE552 pKa = 4.44 FQAQQFGVGDD562 pKa = 3.49 II563 pKa = 4.15
Molecular weight: 61.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.77
IPC_protein 3.834
Toseland 3.592
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.49
Solomon 3.846
Lehninger 3.795
Nozaki 3.948
DTASelect 4.266
Thurlkill 3.656
EMBOSS 3.808
Sillero 3.961
Patrickios 1.914
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A093KQX7|A0A093KQX7_FULGA DENN domain-containing protein 4C OS=Fulmarus glacialis OX=30455 GN=N327_12531 PE=4 SV=1
TT1 pKa = 7.02 HH2 pKa = 7.43 ANSPAAVTHH11 pKa = 5.8 ARR13 pKa = 11.84 GPAVTRR19 pKa = 11.84 ASSPAVTPARR29 pKa = 11.84 SPAVTRR35 pKa = 11.84 ASSPAVTPARR45 pKa = 11.84 SPAVTHH51 pKa = 6.67 ASSPAVTRR59 pKa = 11.84 ASSPVVTPARR69 pKa = 11.84 SPAVTHH75 pKa = 6.67 ASSPAVTHH83 pKa = 6.02 ARR85 pKa = 11.84 SPAVTRR91 pKa = 11.84 ASSPAVTHH99 pKa = 6.18 ARR101 pKa = 11.84 GPAVTRR107 pKa = 11.84 AGRR110 pKa = 11.84 PSS112 pKa = 2.94
Molecular weight: 10.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.544
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.281
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.236
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8115
0
8115
3363607
31
5583
414.5
46.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.352 ± 0.022
2.231 ± 0.025
5.038 ± 0.021
7.159 ± 0.032
3.94 ± 0.018
5.802 ± 0.03
2.536 ± 0.012
5.051 ± 0.02
6.479 ± 0.03
9.716 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.283 ± 0.011
4.199 ± 0.019
5.251 ± 0.034
4.669 ± 0.028
5.157 ± 0.025
8.23 ± 0.038
5.397 ± 0.019
6.314 ± 0.02
1.18 ± 0.009
3.009 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here