Sulfuriferula multivorans
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3515 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A401JFG3|A0A401JFG3_9PROT 50S ribosomal protein L2 OS=Sulfuriferula multivorans OX=1559896 GN=rplB PE=3 SV=1
MM1 pKa = 7.1 QATATDD7 pKa = 3.77 EE8 pKa = 4.53 AGNSYY13 pKa = 10.45 LVYY16 pKa = 10.12 WIYY19 pKa = 11.18 EE20 pKa = 4.25 GVKK23 pKa = 10.58 GDD25 pKa = 5.02 DD26 pKa = 4.24 DD27 pKa = 6.31 RR28 pKa = 11.84 PLDD31 pKa = 3.6 WYY33 pKa = 10.3 DD34 pKa = 3.58 YY35 pKa = 11.4 SSDD38 pKa = 3.64 EE39 pKa = 3.79 IDD41 pKa = 3.99 RR42 pKa = 11.84 IEE44 pKa = 4.09 VQQ46 pKa = 3.09
Molecular weight: 5.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.656
IPC_protein 3.567
Toseland 3.376
ProMoST 3.783
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.579
Rodwell 3.414
Grimsley 3.287
Solomon 3.554
Lehninger 3.503
Nozaki 3.732
DTASelect 3.948
Thurlkill 3.465
EMBOSS 3.579
Sillero 3.706
Patrickios 0.604
IPC_peptide 3.541
IPC2_peptide 3.668
IPC2.peptide.svr19 3.682
Protein with the highest isoelectric point:
>tr|A0A401JBI4|A0A401JBI4_9PROT DNA repair protein RadA OS=Sulfuriferula multivorans OX=1559896 GN=radA PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.6 QPSVIRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.64 RR14 pKa = 11.84 THH16 pKa = 5.96 GFLVRR21 pKa = 11.84 MRR23 pKa = 11.84 TKK25 pKa = 10.44 GGRR28 pKa = 11.84 AVIRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.08 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LAVV44 pKa = 3.42
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3515
0
3515
1063724
37
3404
302.6
33.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.341 ± 0.05
0.922 ± 0.015
5.327 ± 0.028
5.402 ± 0.039
3.687 ± 0.025
7.65 ± 0.042
2.523 ± 0.026
5.373 ± 0.032
3.873 ± 0.042
11.007 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.645 ± 0.023
3.277 ± 0.03
4.795 ± 0.029
4.164 ± 0.03
6.146 ± 0.038
5.471 ± 0.034
5.205 ± 0.032
7.12 ± 0.032
1.369 ± 0.018
2.704 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here