Streptomyces sp. BK335

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10182 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R3IR95|A0A4R3IR95_9ACTN PhzF family phenazine biosynthesis protein OS=Streptomyces sp. BK335 OX=2512168 GN=EV585_107276 PE=4 SV=1
MM1 pKa = 6.49QQEE4 pKa = 3.99AVADD8 pKa = 4.12GGEE11 pKa = 4.1LEE13 pKa = 4.22VWIDD17 pKa = 3.51QDD19 pKa = 3.99LCTGDD24 pKa = 5.01GICAQYY30 pKa = 10.9APEE33 pKa = 4.23VFEE36 pKa = 5.9LDD38 pKa = 3.05IDD40 pKa = 3.65GLAYY44 pKa = 10.67VKK46 pKa = 10.82GAGDD50 pKa = 4.26EE51 pKa = 4.22LLQAPGATTPVPLPLLTDD69 pKa = 3.6VVDD72 pKa = 4.12SAKK75 pKa = 10.15EE76 pKa = 3.93CPGEE80 pKa = 4.38CIHH83 pKa = 6.24VRR85 pKa = 11.84RR86 pKa = 11.84VSDD89 pKa = 3.3RR90 pKa = 11.84VEE92 pKa = 4.16VYY94 pKa = 10.89GPDD97 pKa = 3.45AEE99 pKa = 4.31

Molecular weight: 10.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3IWN8|A0A4R3IWN8_9ACTN Isobutyryl-CoA mutase small subunit OS=Streptomyces sp. BK335 OX=2512168 GN=EV585_105104 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.72HH17 pKa = 5.74RR18 pKa = 11.84KK19 pKa = 7.77LLKK22 pKa = 8.07RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10182

0

10182

3289405

27

5717

323.1

34.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.251 ± 0.035

0.812 ± 0.007

5.941 ± 0.021

5.516 ± 0.027

2.751 ± 0.015

9.323 ± 0.023

2.386 ± 0.011

3.183 ± 0.016

2.182 ± 0.021

10.281 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.725 ± 0.01

1.862 ± 0.015

6.052 ± 0.022

2.844 ± 0.015

8.021 ± 0.03

5.253 ± 0.021

6.385 ± 0.026

8.485 ± 0.024

1.573 ± 0.012

2.174 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski