Amino acid dipepetide frequency for Streptomyces sp. BK335

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.147AlaAla: 20.147 ± 0.13
1.076AlaCys: 1.076 ± 0.017
8.099AlaAsp: 8.099 ± 0.051
8.313AlaGlu: 8.313 ± 0.076
3.517AlaPhe: 3.517 ± 0.038
12.489AlaGly: 12.489 ± 0.07
2.996AlaHis: 2.996 ± 0.03
3.513AlaIle: 3.513 ± 0.034
2.897AlaLys: 2.897 ± 0.04
14.231AlaLeu: 14.231 ± 0.099
2.415AlaMet: 2.415 ± 0.029
2.014AlaAsn: 2.014 ± 0.027
6.777AlaPro: 6.777 ± 0.053
3.836AlaGln: 3.836 ± 0.041
10.148AlaArg: 10.148 ± 0.069
6.222AlaSer: 6.222 ± 0.049
7.122AlaThr: 7.122 ± 0.063
12.044AlaVal: 12.044 ± 0.074
1.894AlaTrp: 1.894 ± 0.027
2.765AlaTyr: 2.765 ± 0.03
0.0AlaXaa: 0.0 ± 0.0
Cys
1.083CysAla: 1.083 ± 0.018
0.099CysCys: 0.099 ± 0.006
0.46CysAsp: 0.46 ± 0.011
0.414CysGlu: 0.414 ± 0.012
0.232CysPhe: 0.232 ± 0.009
0.97CysGly: 0.97 ± 0.017
0.211CysHis: 0.211 ± 0.007
0.167CysIle: 0.167 ± 0.007
0.12CysLys: 0.12 ± 0.007
0.797CysLeu: 0.797 ± 0.017
0.133CysMet: 0.133 ± 0.006
0.147CysAsn: 0.147 ± 0.006
0.514CysPro: 0.514 ± 0.013
0.179CysGln: 0.179 ± 0.008
0.603CysArg: 0.603 ± 0.016
0.452CysSer: 0.452 ± 0.014
0.54CysThr: 0.54 ± 0.014
0.686CysVal: 0.686 ± 0.014
0.14CysTrp: 0.14 ± 0.007
0.171CysTyr: 0.171 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.375AspAla: 7.375 ± 0.053
0.434AspCys: 0.434 ± 0.011
3.526AspAsp: 3.526 ± 0.038
3.74AspGlu: 3.74 ± 0.043
1.698AspPhe: 1.698 ± 0.024
6.312AspGly: 6.312 ± 0.055
1.457AspHis: 1.457 ± 0.019
1.885AspIle: 1.885 ± 0.025
1.226AspLys: 1.226 ± 0.023
6.23AspLeu: 6.23 ± 0.043
0.825AspMet: 0.825 ± 0.014
1.042AspAsn: 1.042 ± 0.019
4.41AspPro: 4.41 ± 0.042
1.576AspGln: 1.576 ± 0.023
4.744AspArg: 4.744 ± 0.043
2.603AspSer: 2.603 ± 0.031
3.333AspThr: 3.333 ± 0.033
4.798AspVal: 4.798 ± 0.047
1.036AspTrp: 1.036 ± 0.019
1.156AspTyr: 1.156 ± 0.021
0.0AspXaa: 0.0 ± 0.0
Glu
7.068GluAla: 7.068 ± 0.067
0.357GluCys: 0.357 ± 0.011
2.774GluAsp: 2.774 ± 0.03
3.523GluGlu: 3.523 ± 0.04
1.455GluPhe: 1.455 ± 0.022
4.137GluGly: 4.137 ± 0.041
1.54GluHis: 1.54 ± 0.022
2.175GluIle: 2.175 ± 0.03
1.359GluLys: 1.359 ± 0.024
6.572GluLeu: 6.572 ± 0.059
0.888GluMet: 0.888 ± 0.015
1.008GluAsn: 1.008 ± 0.021
3.218GluPro: 3.218 ± 0.035
2.232GluGln: 2.232 ± 0.027
5.3GluArg: 5.3 ± 0.053
2.435GluSer: 2.435 ± 0.029
2.89GluThr: 2.89 ± 0.032
4.309GluVal: 4.309 ± 0.043
0.801GluTrp: 0.801 ± 0.015
1.114GluTyr: 1.114 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
3.571PheAla: 3.571 ± 0.034
0.275PheCys: 0.275 ± 0.009
1.92PheAsp: 1.92 ± 0.025
1.423PheGlu: 1.423 ± 0.022
0.886PhePhe: 0.886 ± 0.019
3.033PheGly: 3.033 ± 0.035
0.659PheHis: 0.659 ± 0.014
0.673PheIle: 0.673 ± 0.015
0.52PheLys: 0.52 ± 0.012
2.61PheLeu: 2.61 ± 0.031
0.416PheMet: 0.416 ± 0.012
0.601PheAsn: 0.601 ± 0.015
1.406PhePro: 1.406 ± 0.022
0.717PheGln: 0.717 ± 0.015
1.857PheArg: 1.857 ± 0.022
1.525PheSer: 1.525 ± 0.024
2.104PheThr: 2.104 ± 0.025
2.263PheVal: 2.263 ± 0.029
0.442PheTrp: 0.442 ± 0.011
0.608PheTyr: 0.608 ± 0.015
0.0PheXaa: 0.0 ± 0.0
Gly
10.665GlyAla: 10.665 ± 0.071
0.839GlyCys: 0.839 ± 0.016
5.02GlyAsp: 5.02 ± 0.045
4.885GlyGlu: 4.885 ± 0.041
2.894GlyPhe: 2.894 ± 0.032
8.552GlyGly: 8.552 ± 0.072
2.412GlyHis: 2.412 ± 0.032
3.46GlyIle: 3.46 ± 0.034
2.408GlyLys: 2.408 ± 0.028
9.279GlyLeu: 9.279 ± 0.063
1.966GlyMet: 1.966 ± 0.025
1.825GlyAsn: 1.825 ± 0.029
5.008GlyPro: 5.008 ± 0.043
2.721GlyGln: 2.721 ± 0.034
7.707GlyArg: 7.707 ± 0.059
5.468GlySer: 5.468 ± 0.048
6.587GlyThr: 6.587 ± 0.046
7.478GlyVal: 7.478 ± 0.05
1.74GlyTrp: 1.74 ± 0.023
2.32GlyTyr: 2.32 ± 0.029
0.0GlyXaa: 0.0 ± 0.0
His
2.776HisAla: 2.776 ± 0.034
0.218HisCys: 0.218 ± 0.008
1.392HisAsp: 1.392 ± 0.02
1.261HisGlu: 1.261 ± 0.019
0.665HisPhe: 0.665 ± 0.015
2.469HisGly: 2.469 ± 0.031
0.747HisHis: 0.747 ± 0.016
0.728HisIle: 0.728 ± 0.014
0.376HisLys: 0.376 ± 0.009
2.511HisLeu: 2.511 ± 0.029
0.338HisMet: 0.338 ± 0.011
0.41HisAsn: 0.41 ± 0.011
1.877HisPro: 1.877 ± 0.026
0.672HisGln: 0.672 ± 0.015
2.204HisArg: 2.204 ± 0.026
1.07HisSer: 1.07 ± 0.018
1.459HisThr: 1.459 ± 0.021
1.746HisVal: 1.746 ± 0.02
0.411HisTrp: 0.411 ± 0.012
0.53HisTyr: 0.53 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
4.798IleAla: 4.798 ± 0.043
0.297IleCys: 0.297 ± 0.01
2.234IleAsp: 2.234 ± 0.025
1.903IleGlu: 1.903 ± 0.027
0.689IlePhe: 0.689 ± 0.017
3.483IleGly: 3.483 ± 0.033
0.635IleHis: 0.635 ± 0.013
0.864IleIle: 0.864 ± 0.018
0.724IleLys: 0.724 ± 0.018
2.454IleLeu: 2.454 ± 0.031
0.438IleMet: 0.438 ± 0.012
0.729IleAsn: 0.729 ± 0.017
1.817IlePro: 1.817 ± 0.024
0.727IleGln: 0.727 ± 0.017
2.313IleArg: 2.313 ± 0.03
1.722IleSer: 1.722 ± 0.025
2.352IleThr: 2.352 ± 0.029
2.753IleVal: 2.753 ± 0.031
0.38IleTrp: 0.38 ± 0.012
0.522IleTyr: 0.522 ± 0.014
0.0IleXaa: 0.0 ± 0.0
Lys
2.987LysAla: 2.987 ± 0.038
0.127LysCys: 0.127 ± 0.007
1.338LysAsp: 1.338 ± 0.025
1.142LysGlu: 1.142 ± 0.023
0.434LysPhe: 0.434 ± 0.011
1.892LysGly: 1.892 ± 0.029
0.434LysHis: 0.434 ± 0.013
0.862LysIle: 0.862 ± 0.021
0.89LysLys: 0.89 ± 0.024
1.993LysLeu: 1.993 ± 0.027
0.369LysMet: 0.369 ± 0.011
0.554LysAsn: 0.554 ± 0.016
1.368LysPro: 1.368 ± 0.022
0.73LysGln: 0.73 ± 0.015
1.428LysArg: 1.428 ± 0.021
1.196LysSer: 1.196 ± 0.02
1.337LysThr: 1.337 ± 0.024
1.95LysVal: 1.95 ± 0.026
0.29LysTrp: 0.29 ± 0.009
0.499LysTyr: 0.499 ± 0.013
0.0LysXaa: 0.0 ± 0.0
Leu
14.736LeuAla: 14.736 ± 0.091
0.874LeuCys: 0.874 ± 0.016
6.558LeuAsp: 6.558 ± 0.049
4.63LeuGlu: 4.63 ± 0.046
2.577LeuPhe: 2.577 ± 0.034
9.207LeuGly: 9.207 ± 0.06
2.404LeuHis: 2.404 ± 0.028
3.292LeuIle: 3.292 ± 0.037
2.054LeuLys: 2.054 ± 0.029
11.123LeuLeu: 11.123 ± 0.088
1.637LeuMet: 1.637 ± 0.022
1.755LeuAsn: 1.755 ± 0.023
6.474LeuPro: 6.474 ± 0.045
2.324LeuGln: 2.324 ± 0.029
8.612LeuArg: 8.612 ± 0.067
5.375LeuSer: 5.375 ± 0.038
7.102LeuThr: 7.102 ± 0.049
8.798LeuVal: 8.798 ± 0.053
1.355LeuTrp: 1.355 ± 0.022
1.923LeuTyr: 1.923 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.215MetAla: 2.215 ± 0.024
0.138MetCys: 0.138 ± 0.006
0.893MetAsp: 0.893 ± 0.015
0.715MetGlu: 0.715 ± 0.016
0.451MetPhe: 0.451 ± 0.012
1.313MetGly: 1.313 ± 0.022
0.359MetHis: 0.359 ± 0.01
0.671MetIle: 0.671 ± 0.014
0.424MetLys: 0.424 ± 0.011
1.638MetLeu: 1.638 ± 0.024
0.289MetMet: 0.289 ± 0.01
0.466MetAsn: 0.466 ± 0.012
1.136MetPro: 1.136 ± 0.018
0.434MetGln: 0.434 ± 0.013
1.385MetArg: 1.385 ± 0.02
1.323MetSer: 1.323 ± 0.02
1.606MetThr: 1.606 ± 0.022
1.264MetVal: 1.264 ± 0.022
0.219MetTrp: 0.219 ± 0.008
0.316MetTyr: 0.316 ± 0.01
0.0MetXaa: 0.0 ± 0.0
Asn
2.318AsnAla: 2.318 ± 0.024
0.175AsnCys: 0.175 ± 0.008
1.017AsnAsp: 1.017 ± 0.019
0.814AsnGlu: 0.814 ± 0.015
0.491AsnPhe: 0.491 ± 0.014
2.045AsnGly: 2.045 ± 0.033
0.415AsnHis: 0.415 ± 0.011
0.666AsnIle: 0.666 ± 0.015
0.443AsnLys: 0.443 ± 0.012
1.7AsnLeu: 1.7 ± 0.029
0.3AsnMet: 0.3 ± 0.009
0.479AsnAsn: 0.479 ± 0.014
1.406AsnPro: 1.406 ± 0.025
0.573AsnGln: 0.573 ± 0.014
1.336AsnArg: 1.336 ± 0.023
1.038AsnSer: 1.038 ± 0.025
1.221AsnThr: 1.221 ± 0.024
1.431AsnVal: 1.431 ± 0.024
0.324AsnTrp: 0.324 ± 0.012
0.43AsnTyr: 0.43 ± 0.012
0.0AsnXaa: 0.0 ± 0.0
Pro
8.307ProAla: 8.307 ± 0.071
0.361ProCys: 0.361 ± 0.011
4.427ProAsp: 4.427 ± 0.041
4.104ProGlu: 4.104 ± 0.039
1.532ProPhe: 1.532 ± 0.021
6.425ProGly: 6.425 ± 0.05
1.478ProHis: 1.478 ± 0.023
1.31ProIle: 1.31 ± 0.022
1.201ProLys: 1.201 ± 0.023
5.398ProLeu: 5.398 ± 0.036
0.977ProMet: 0.977 ± 0.018
0.976ProAsn: 0.976 ± 0.023
3.604ProPro: 3.604 ± 0.053
1.785ProGln: 1.785 ± 0.029
4.011ProArg: 4.011 ± 0.039
3.412ProSer: 3.412 ± 0.038
3.363ProThr: 3.363 ± 0.035
5.44ProVal: 5.44 ± 0.046
0.936ProTrp: 0.936 ± 0.017
1.467ProTyr: 1.467 ± 0.023
0.0ProXaa: 0.0 ± 0.0
Gln
3.742GlnAla: 3.742 ± 0.038
0.186GlnCys: 0.186 ± 0.008
1.464GlnAsp: 1.464 ± 0.024
1.439GlnGlu: 1.439 ± 0.022
0.71GlnPhe: 0.71 ± 0.015
2.353GlnGly: 2.353 ± 0.03
0.718GlnHis: 0.718 ± 0.015
1.101GlnIle: 1.101 ± 0.018
0.641GlnLys: 0.641 ± 0.016
3.116GlnLeu: 3.116 ± 0.032
0.492GlnMet: 0.492 ± 0.015
0.527GlnAsn: 0.527 ± 0.014
1.798GlnPro: 1.798 ± 0.031
1.312GlnGln: 1.312 ± 0.029
2.385GlnArg: 2.385 ± 0.025
1.38GlnSer: 1.38 ± 0.023
1.512GlnThr: 1.512 ± 0.023
2.43GlnVal: 2.43 ± 0.03
0.489GlnTrp: 0.489 ± 0.014
0.644GlnTyr: 0.644 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
9.722ArgAla: 9.722 ± 0.081
0.609ArgCys: 0.609 ± 0.014
4.158ArgAsp: 4.158 ± 0.04
4.659ArgGlu: 4.659 ± 0.048
2.365ArgPhe: 2.365 ± 0.03
5.634ArgGly: 5.634 ± 0.047
2.192ArgHis: 2.192 ± 0.024
3.213ArgIle: 3.213 ± 0.028
1.624ArgLys: 1.624 ± 0.025
8.978ArgLeu: 8.978 ± 0.067
1.692ArgMet: 1.692 ± 0.022
1.333ArgAsn: 1.333 ± 0.022
5.005ArgPro: 5.005 ± 0.048
2.424ArgGln: 2.424 ± 0.027
7.848ArgArg: 7.848 ± 0.064
4.099ArgSer: 4.099 ± 0.033
5.476ArgThr: 5.476 ± 0.042
5.923ArgVal: 5.923 ± 0.05
1.399ArgTrp: 1.399 ± 0.023
1.854ArgTyr: 1.854 ± 0.025
0.0ArgXaa: 0.0 ± 0.0
Ser
6.946SerAla: 6.946 ± 0.056
0.448SerCys: 0.448 ± 0.014
2.873SerAsp: 2.873 ± 0.035
2.342SerGlu: 2.342 ± 0.027
1.565SerPhe: 1.565 ± 0.024
6.116SerGly: 6.116 ± 0.057
1.046SerHis: 1.046 ± 0.021
1.439SerIle: 1.439 ± 0.023
1.071SerLys: 1.071 ± 0.02
4.951SerLeu: 4.951 ± 0.038
1.111SerMet: 1.111 ± 0.018
0.976SerAsn: 0.976 ± 0.018
3.295SerPro: 3.295 ± 0.037
1.318SerGln: 1.318 ± 0.025
3.72SerArg: 3.72 ± 0.033
3.199SerSer: 3.199 ± 0.051
3.328SerThr: 3.328 ± 0.041
4.454SerVal: 4.454 ± 0.034
0.966SerTrp: 0.966 ± 0.02
1.362SerTyr: 1.362 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
9.16ThrAla: 9.16 ± 0.064
0.478ThrCys: 0.478 ± 0.014
3.766ThrAsp: 3.766 ± 0.04
3.284ThrGlu: 3.284 ± 0.036
1.696ThrPhe: 1.696 ± 0.025
6.802ThrGly: 6.802 ± 0.05
1.317ThrHis: 1.317 ± 0.02
1.806ThrIle: 1.806 ± 0.03
1.239ThrLys: 1.239 ± 0.023
5.886ThrLeu: 5.886 ± 0.047
0.98ThrMet: 0.98 ± 0.018
1.119ThrAsn: 1.119 ± 0.025
4.313ThrPro: 4.313 ± 0.044
1.449ThrGln: 1.449 ± 0.023
4.121ThrArg: 4.121 ± 0.034
3.493ThrSer: 3.493 ± 0.047
4.181ThrThr: 4.181 ± 0.056
6.3ThrVal: 6.3 ± 0.05
0.989ThrTrp: 0.989 ± 0.02
1.475ThrTyr: 1.475 ± 0.022
0.0ThrXaa: 0.0 ± 0.0
Val
10.462ValAla: 10.462 ± 0.06
0.783ValCys: 0.783 ± 0.016
4.942ValAsp: 4.942 ± 0.047
4.576ValGlu: 4.576 ± 0.044
2.456ValPhe: 2.456 ± 0.027
6.482ValGly: 6.482 ± 0.053
2.02ValHis: 2.02 ± 0.026
2.896ValIle: 2.896 ± 0.029
1.779ValLys: 1.779 ± 0.026
9.42ValLeu: 9.42 ± 0.062
1.426ValMet: 1.426 ± 0.018
1.762ValAsn: 1.762 ± 0.028
5.185ValPro: 5.185 ± 0.048
2.144ValGln: 2.144 ± 0.026
7.26ValArg: 7.26 ± 0.059
4.464ValSer: 4.464 ± 0.034
5.91ValThr: 5.91 ± 0.052
8.011ValVal: 8.011 ± 0.063
1.196ValTrp: 1.196 ± 0.021
1.677ValTyr: 1.677 ± 0.022
0.0ValXaa: 0.0 ± 0.0
Trp
1.671TrpAla: 1.671 ± 0.025
0.153TrpCys: 0.153 ± 0.008
0.892TrpAsp: 0.892 ± 0.021
0.719TrpGlu: 0.719 ± 0.017
0.519TrpPhe: 0.519 ± 0.011
1.101TrpGly: 1.101 ± 0.019
0.403TrpHis: 0.403 ± 0.011
0.595TrpIle: 0.595 ± 0.014
0.395TrpLys: 0.395 ± 0.011
1.801TrpLeu: 1.801 ± 0.027
0.294TrpMet: 0.294 ± 0.008
0.441TrpAsn: 0.441 ± 0.015
0.802TrpPro: 0.802 ± 0.017
0.643TrpGln: 0.643 ± 0.013
1.403TrpArg: 1.403 ± 0.021
1.009TrpSer: 1.009 ± 0.02
1.12TrpThr: 1.12 ± 0.02
1.0TrpVal: 1.0 ± 0.019
0.361TrpTrp: 0.361 ± 0.01
0.408TrpTyr: 0.408 ± 0.011
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.765TyrAla: 2.765 ± 0.033
0.192TyrCys: 0.192 ± 0.008
1.653TyrAsp: 1.653 ± 0.031
1.269TyrGlu: 1.269 ± 0.023
0.676TyrPhe: 0.676 ± 0.015
2.417TyrGly: 2.417 ± 0.03
0.416TyrHis: 0.416 ± 0.014
0.517TyrIle: 0.517 ± 0.014
0.429TyrLys: 0.429 ± 0.013
2.122TyrLeu: 2.122 ± 0.027
0.268TyrMet: 0.268 ± 0.011
0.457TyrAsn: 0.457 ± 0.014
1.104TyrPro: 1.104 ± 0.021
0.642TyrGln: 0.642 ± 0.015
1.825TyrArg: 1.825 ± 0.026
1.041TyrSer: 1.041 ± 0.02
1.309TyrThr: 1.309 ± 0.019
1.775TyrVal: 1.775 ± 0.026
0.364TyrTrp: 0.364 ± 0.01
0.496TyrTyr: 0.496 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 10182 proteins (3289405 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski