Oxalobacteraceae bacterium OM1
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4547 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y9RL17|A0A4Y9RL17_9BURK Tol-pal system-associated acyl-CoA thioesterase OS=Oxalobacteraceae bacterium OM1 OX=2561929 GN=ybgC PE=3 SV=1
MM1 pKa = 7.19 TLFYY5 pKa = 9.89 PIYY8 pKa = 9.2 PISSTAGPSPCPNDD22 pKa = 3.38 SDD24 pKa = 4.16 VVPWYY29 pKa = 10.74 VRR31 pKa = 11.84 VPHH34 pKa = 6.86 KK35 pKa = 10.92 LPAAPRR41 pKa = 11.84 ASMQDD46 pKa = 3.35 DD47 pKa = 4.04 LPFEE51 pKa = 4.3 FSEE54 pKa = 4.79 PGSAGQDD61 pKa = 3.41 SDD63 pKa = 6.0 DD64 pKa = 4.51 EE65 pKa = 4.29 EE66 pKa = 6.59 ADD68 pKa = 3.37
Molecular weight: 7.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.541
ProMoST 3.846
Dawson 3.757
Bjellqvist 3.999
Wikipedia 3.719
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.872
Patrickios 1.888
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|A0A4Y9QFC2|A0A4Y9QFC2_9BURK OmpA family protein (Fragment) OS=Oxalobacteraceae bacterium OM1 OX=2561929 GN=E4K72_21520 PE=4 SV=1
MM1 pKa = 7.49 RR2 pKa = 11.84 PSVRR6 pKa = 11.84 SPRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 SAHH14 pKa = 5.55 SARR17 pKa = 11.84 QTSRR21 pKa = 11.84 RR22 pKa = 11.84 SRR24 pKa = 11.84 QRR26 pKa = 11.84 RR27 pKa = 11.84 SRR29 pKa = 11.84 RR30 pKa = 11.84 CPHH33 pKa = 6.23 RR34 pKa = 11.84 RR35 pKa = 11.84 SLPSARR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 LRR45 pKa = 11.84 PSRR48 pKa = 11.84 RR49 pKa = 11.84 PTSPCSPRR57 pKa = 11.84 RR58 pKa = 11.84 RR59 pKa = 11.84 SPNSVPLSSVPP70 pKa = 3.42
Molecular weight: 8.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.484
IPC2_protein 11.067
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.34
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.062
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4547
0
4547
1366726
24
3559
300.6
32.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.296 ± 0.045
0.939 ± 0.013
5.374 ± 0.026
5.463 ± 0.04
3.719 ± 0.027
7.944 ± 0.039
2.315 ± 0.02
4.794 ± 0.025
3.657 ± 0.033
10.349 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.533 ± 0.018
2.94 ± 0.026
4.979 ± 0.026
3.838 ± 0.023
6.815 ± 0.04
5.29 ± 0.029
5.335 ± 0.043
7.593 ± 0.031
1.335 ± 0.016
2.493 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here