Streptomyces spectabilis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7680 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516RJZ8|A0A516RJZ8_STRST RNA polymerase sigma factor OS=Streptomyces spectabilis OX=68270 GN=FH965_40190 PE=3 SV=1
MM1 pKa = 6.93ATLMAALTLAAVTLDD16 pKa = 3.52AAHH19 pKa = 6.94AGAVPPASNCFVNGQPQSGPEE40 pKa = 3.78IYY42 pKa = 9.17GTAGDD47 pKa = 4.55DD48 pKa = 5.18DD49 pKa = 4.1IRR51 pKa = 11.84CDD53 pKa = 3.52RR54 pKa = 11.84LVSGDD59 pKa = 4.09VIFAYY64 pKa = 10.2QGNDD68 pKa = 3.6TIRR71 pKa = 11.84VTFNYY76 pKa = 10.35AGVINGGNGQDD87 pKa = 3.67TIRR90 pKa = 11.84LEE92 pKa = 4.22EE93 pKa = 4.42EE94 pKa = 3.74NTGLIQAGDD103 pKa = 3.66GSDD106 pKa = 5.02DD107 pKa = 4.23VIASYY112 pKa = 10.4NAHH115 pKa = 6.74HH116 pKa = 6.7GGIHH120 pKa = 6.71GNTGDD125 pKa = 5.89DD126 pKa = 4.15EE127 pKa = 4.69IQVLLNEE134 pKa = 4.59GNVDD138 pKa = 3.92GGPGNDD144 pKa = 2.86VCRR147 pKa = 11.84VNEE150 pKa = 4.85GIVLNCNPP158 pKa = 4.22

Molecular weight: 16.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516RAK3|A0A516RAK3_STRST PH domain-containing protein OS=Streptomyces spectabilis OX=68270 GN=CP982_22440 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.86GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7680

0

7680

2715784

29

7603

353.6

37.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.197 ± 0.042

0.793 ± 0.007

6.108 ± 0.025

5.607 ± 0.026

2.7 ± 0.017

9.576 ± 0.028

2.358 ± 0.014

2.807 ± 0.022

2.163 ± 0.029

10.447 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.616 ± 0.013

1.589 ± 0.015

6.184 ± 0.027

2.62 ± 0.019

8.342 ± 0.029

4.831 ± 0.02

5.959 ± 0.024

8.582 ± 0.032

1.494 ± 0.011

2.028 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski