Limnochorda pilosa

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Limnochordia; Limnochordales; Limnochordaceae; Limnochorda

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3599 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2SMV9|A0A0K2SMV9_9FIRM Protein translocase subunit SecD OS=Limnochorda pilosa OX=1555112 GN=secD PE=3 SV=1
MM1 pKa = 7.66SDD3 pKa = 3.28VKK5 pKa = 11.0QKK7 pKa = 10.64LAYY10 pKa = 10.07ARR12 pKa = 11.84GLLEE16 pKa = 4.76GRR18 pKa = 11.84GTDD21 pKa = 3.46GASGSEE27 pKa = 4.13PVWTSVLEE35 pKa = 4.34ALDD38 pKa = 3.99SLDD41 pKa = 3.63AAVEE45 pKa = 3.92AMRR48 pKa = 11.84GDD50 pKa = 3.76LEE52 pKa = 4.06EE53 pKa = 5.43LEE55 pKa = 5.12EE56 pKa = 4.08YY57 pKa = 9.71VQAVDD62 pKa = 5.5DD63 pKa = 4.69DD64 pKa = 4.79LFDD67 pKa = 4.54VEE69 pKa = 4.79ADD71 pKa = 3.32LYY73 pKa = 11.18GYY75 pKa = 10.67DD76 pKa = 3.81EE77 pKa = 6.32ADD79 pKa = 3.47MTEE82 pKa = 4.78LEE84 pKa = 4.97CPSCHH89 pKa = 6.02TPLVVEE95 pKa = 4.65AEE97 pKa = 4.11FLEE100 pKa = 5.24DD101 pKa = 3.13EE102 pKa = 4.64ASEE105 pKa = 4.62VTCPEE110 pKa = 4.48CGHH113 pKa = 6.18VIHH116 pKa = 7.34RR117 pKa = 11.84GPLYY121 pKa = 10.28QGTSQEE127 pKa = 4.21VAGAPTADD135 pKa = 3.51GASRR139 pKa = 3.45

Molecular weight: 14.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2SJS3|A0A0K2SJS3_9FIRM Heme exporter protein B OS=Limnochorda pilosa OX=1555112 GN=LIP_1406 PE=3 SV=1
MM1 pKa = 7.51PAVALPLLLAGLAAGRR17 pKa = 11.84TPTGPPGFVFPPVSGMFQRR36 pKa = 11.84PRR38 pKa = 11.84LALPNARR45 pKa = 11.84TAGRR49 pKa = 11.84LTRR52 pKa = 11.84PRR54 pKa = 11.84FASSRR59 pKa = 11.84PLWW62 pKa = 3.76

Molecular weight: 6.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3599

0

3599

1121070

43

3410

311.5

33.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.246 ± 0.05

0.625 ± 0.013

4.655 ± 0.031

6.832 ± 0.044

3.164 ± 0.029

9.559 ± 0.042

1.994 ± 0.016

3.504 ± 0.028

1.749 ± 0.021

11.593 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.97 ± 0.02

1.723 ± 0.02

6.057 ± 0.037

3.242 ± 0.023

8.963 ± 0.047

4.75 ± 0.027

4.696 ± 0.029

8.851 ± 0.035

1.574 ± 0.019

2.255 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski