Flavobacterium succinicans
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2812 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A199XUI8|A0A199XUI8_9FLAO 1 4-dihydroxy-2-naphthoate octaprenyltransferase OS=Flavobacterium succinicans OX=29536 GN=menA PE=3 SV=1
MM1 pKa = 7.39 LAIIIPYY8 pKa = 9.47 YY9 pKa = 10.75 KK10 pKa = 9.94 LTFFEE15 pKa = 4.71 ATLQSLAAQTCQDD28 pKa = 3.46 FKK30 pKa = 11.83 VYY32 pKa = 10.29 IGDD35 pKa = 3.86 DD36 pKa = 3.56 ASPEE40 pKa = 4.12 KK41 pKa = 9.65 PTEE44 pKa = 3.97 LLEE47 pKa = 4.44 HH48 pKa = 6.28 FQGQFDD54 pKa = 4.0 FVYY57 pKa = 10.61 HH58 pKa = 6.43 RR59 pKa = 11.84 FEE61 pKa = 4.5 FNLGGVSLTQQWEE74 pKa = 4.01 RR75 pKa = 11.84 CIALSDD81 pKa = 3.77 NEE83 pKa = 4.33 PWLMILGDD91 pKa = 4.37 DD92 pKa = 4.59 DD93 pKa = 4.63 VLGEE97 pKa = 4.19 NVVEE101 pKa = 4.26 AFYY104 pKa = 11.66 NNN106 pKa = 3.21
Molecular weight: 12.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.836
IPC2_protein 4.088
IPC_protein 3.986
Toseland 3.808
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.872
Rodwell 3.834
Grimsley 3.719
Solomon 3.948
Lehninger 3.91
Nozaki 4.088
DTASelect 4.253
Thurlkill 3.846
EMBOSS 3.884
Sillero 4.113
Patrickios 0.896
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 3.992
Protein with the highest isoelectric point:
>tr|A0A199XU36|A0A199XU36_9FLAO IMPACT family member YigZ OS=Flavobacterium succinicans OX=29536 GN=yigZ PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.91 HH17 pKa = 4.39 GFMDD21 pKa = 4.5 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.19 GRR40 pKa = 11.84 HH41 pKa = 5.41 KK42 pKa = 10.14 LTVSSEE48 pKa = 3.92 PRR50 pKa = 11.84 HH51 pKa = 5.77 KK52 pKa = 10.61 KK53 pKa = 9.84
Molecular weight: 6.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.252
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.974
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.063
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2812
0
2812
957848
29
3329
340.6
38.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.835 ± 0.045
0.758 ± 0.015
5.082 ± 0.035
6.225 ± 0.049
5.436 ± 0.041
6.335 ± 0.047
1.699 ± 0.021
7.844 ± 0.045
7.979 ± 0.057
9.306 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.172 ± 0.022
6.196 ± 0.048
3.469 ± 0.023
3.657 ± 0.026
3.135 ± 0.029
6.398 ± 0.034
6.109 ± 0.057
6.333 ± 0.038
1.046 ± 0.018
3.986 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here