Tritrichomonas foetus
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 24634 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1J4KJT4|A0A1J4KJT4_9EUKA Uncharacterized protein OS=Tritrichomonas foetus OX=1144522 GN=TRFO_21408 PE=4 SV=1
MM1 pKa = 7.36 NNDD4 pKa = 5.28 LINDD8 pKa = 4.36 RR9 pKa = 11.84 ANKK12 pKa = 9.81 LLPNNIGPHH21 pKa = 6.36 NINDD25 pKa = 4.75 LDD27 pKa = 4.01 LQQIKK32 pKa = 10.29 PPDD35 pKa = 3.73 DD36 pKa = 4.83 NYY38 pKa = 11.66 DD39 pKa = 3.23 NDD41 pKa = 4.06 YY42 pKa = 11.2 QADD45 pKa = 4.04 TQNPSTVDD53 pKa = 3.41 PTHH56 pKa = 6.76 EE57 pKa = 4.12 SAANIPTNEE66 pKa = 4.06 INDD69 pKa = 3.77 DD70 pKa = 3.63 SSIYY74 pKa = 10.42 DD75 pKa = 3.32 SNYY78 pKa = 9.71 DD79 pKa = 3.34 SNYY82 pKa = 9.46 YY83 pKa = 7.71 STNYY87 pKa = 9.62 EE88 pKa = 3.69 STYY91 pKa = 11.19 ASTAATTANSILVNGKK107 pKa = 8.8 SGRR110 pKa = 11.84 RR111 pKa = 11.84 YY112 pKa = 10.53 DD113 pKa = 3.64 SAGTDD118 pKa = 3.38 VPINNDD124 pKa = 3.19 PSDD127 pKa = 3.26
Molecular weight: 13.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.719
IPC_protein 3.732
Toseland 3.49
ProMoST 3.884
Dawson 3.757
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.554
Grimsley 3.401
Solomon 3.745
Lehninger 3.706
Nozaki 3.884
DTASelect 4.19
Thurlkill 3.579
EMBOSS 3.757
Sillero 3.859
Patrickios 0.655
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.811
Protein with the highest isoelectric point:
>tr|A0A1J4JFQ1|A0A1J4JFQ1_9EUKA Uncharacterized protein OS=Tritrichomonas foetus OX=1144522 GN=TRFO_37357 PE=4 SV=1
MM1 pKa = 7.84 RR2 pKa = 11.84 IIRR5 pKa = 11.84 MKK7 pKa = 9.82 IIRR10 pKa = 11.84 MRR12 pKa = 11.84 IIRR15 pKa = 11.84 MRR17 pKa = 11.84 IIRR20 pKa = 11.84 MKK22 pKa = 9.71 IIRR25 pKa = 11.84 MRR27 pKa = 11.84 IIRR30 pKa = 11.84 MRR32 pKa = 11.84 IIRR35 pKa = 11.84 MKK37 pKa = 10.17 IIIMKK42 pKa = 9.35 IIIMRR47 pKa = 11.84 IIGIIIIRR55 pKa = 11.84 MRR57 pKa = 11.84 IGLIQHH63 pKa = 6.1 NVV65 pKa = 2.78
Molecular weight: 8.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.389
IPC_protein 12.983
Toseland 13.144
ProMoST 13.642
Dawson 13.144
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.749
Grimsley 13.188
Solomon 13.642
Lehninger 13.554
Nozaki 13.144
DTASelect 13.144
Thurlkill 13.144
EMBOSS 13.642
Sillero 13.144
Patrickios 12.486
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.284
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24634
0
24634
13073236
29
7078
530.7
60.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.793 ± 0.012
1.567 ± 0.008
5.518 ± 0.009
6.865 ± 0.02
5.722 ± 0.029
3.654 ± 0.014
2.154 ± 0.006
8.356 ± 0.016
7.463 ± 0.019
9.158 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.243 ± 0.006
7.137 ± 0.018
4.194 ± 0.013
4.444 ± 0.016
3.809 ± 0.011
8.505 ± 0.019
5.157 ± 0.01
4.896 ± 0.011
0.743 ± 0.004
3.448 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here