Croceicoccus marinus
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3600 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z1FEV2|A0A1Z1FEV2_9SPHN Tyr recombinase domain-containing protein OS=Croceicoccus marinus OX=450378 GN=A9D14_05040 PE=4 SV=1
MM1 pKa = 7.48 LALAGCGGDD10 pKa = 3.63 QIVSPGTGGDD20 pKa = 3.63 IIIEE24 pKa = 4.29 NPAPAPAPTPSPTPTPTPMVTPAAGCPTINSSGGLTDD61 pKa = 4.4 AGTITGDD68 pKa = 2.94 TGEE71 pKa = 4.36 YY72 pKa = 9.44 RR73 pKa = 11.84 VCTLPASFDD82 pKa = 3.57 EE83 pKa = 4.75 SDD85 pKa = 3.34 NLPYY89 pKa = 10.0 IEE91 pKa = 4.77 GLLYY95 pKa = 10.59 SINGRR100 pKa = 11.84 VDD102 pKa = 2.73 VGTDD106 pKa = 3.31 GGAVPSDD113 pKa = 3.6 ADD115 pKa = 3.53 SDD117 pKa = 4.07 VTLSIEE123 pKa = 4.36 PGAILYY129 pKa = 8.11 GATGRR134 pKa = 11.84 SYY136 pKa = 11.35 LVVNRR141 pKa = 11.84 GNQIDD146 pKa = 4.09 AVGEE150 pKa = 3.73 PDD152 pKa = 3.42 MPIVFTSRR160 pKa = 11.84 DD161 pKa = 3.59 NVLGLSTDD169 pKa = 3.28 GSDD172 pKa = 3.72 AQWGGVVLLGRR183 pKa = 11.84 APVSDD188 pKa = 4.3 CRR190 pKa = 11.84 DD191 pKa = 3.44 GVPNKK196 pKa = 10.24 ANQASCEE203 pKa = 3.94 QALEE207 pKa = 4.32 GAAVEE212 pKa = 4.4 TLFGGNTPGDD222 pKa = 3.6 SSGTMQYY229 pKa = 10.61 FQIRR233 pKa = 11.84 YY234 pKa = 9.88 SGFTLEE240 pKa = 5.43 GGSEE244 pKa = 4.14 LQSLTTGGIGSGTTIDD260 pKa = 3.05 HH261 pKa = 5.9 WMSFNSSDD269 pKa = 4.62 DD270 pKa = 3.31 GTEE273 pKa = 4.01 FFGGRR278 pKa = 11.84 VNMKK282 pKa = 9.47 NVVVVGASDD291 pKa = 4.34 DD292 pKa = 3.98 SLDD295 pKa = 3.57 VDD297 pKa = 4.44 TGAQANLQYY306 pKa = 11.14 VIVAQRR312 pKa = 11.84 ANTGDD317 pKa = 3.94 SIIEE321 pKa = 4.05 LDD323 pKa = 5.24 SPDD326 pKa = 3.72 EE327 pKa = 4.75 DD328 pKa = 4.47 YY329 pKa = 7.86 TTGALPRR336 pKa = 11.84 TNLQIANATFLSRR349 pKa = 11.84 STGSSQALRR358 pKa = 11.84 LRR360 pKa = 11.84 GGAQFSLVNSVIDD373 pKa = 3.49 IEE375 pKa = 4.36 GDD377 pKa = 3.61 RR378 pKa = 11.84 TCVRR382 pKa = 11.84 LDD384 pKa = 3.37 EE385 pKa = 4.57 TVTLAQDD392 pKa = 3.45 PLFASVVADD401 pKa = 4.27 CGTTQPFRR409 pKa = 11.84 GSSGVTDD416 pKa = 4.03 AQVAAAFNDD425 pKa = 3.76 GDD427 pKa = 3.92 DD428 pKa = 3.68 TDD430 pKa = 3.91 AAFAITLTGSFINGTGEE447 pKa = 4.1 TGVAAYY453 pKa = 10.44 NPTALSSYY461 pKa = 10.76 FDD463 pKa = 3.54 DD464 pKa = 3.48 TDD466 pKa = 4.17 YY467 pKa = 11.35 IGAVADD473 pKa = 4.4 QTDD476 pKa = 3.03 TWYY479 pKa = 10.37 EE480 pKa = 3.69 GWTCDD485 pKa = 4.7 SATLTFGNNTGPCTEE500 pKa = 4.49 LPVYY504 pKa = 10.5 SS505 pKa = 4.15
Molecular weight: 51.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.605
IPC_protein 3.656
Toseland 3.414
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.325
Solomon 3.656
Lehninger 3.605
Nozaki 3.77
DTASelect 4.05
Thurlkill 3.478
EMBOSS 3.617
Sillero 3.77
Patrickios 0.846
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.713
Protein with the highest isoelectric point:
>tr|A0A1Z1FDW3|A0A1Z1FDW3_9SPHN Transcription termination factor Rho OS=Croceicoccus marinus OX=450378 GN=rho PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.04 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 SATPGGRR28 pKa = 11.84 NVLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.02 KK41 pKa = 10.61 LSAA44 pKa = 4.03
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3600
0
3600
1160639
38
2329
322.4
34.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.762 ± 0.06
0.841 ± 0.014
6.374 ± 0.042
6.05 ± 0.04
3.596 ± 0.027
8.888 ± 0.038
2.036 ± 0.022
5.02 ± 0.026
2.847 ± 0.032
9.716 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.654 ± 0.023
2.535 ± 0.018
5.254 ± 0.027
3.201 ± 0.023
7.379 ± 0.038
5.318 ± 0.027
4.971 ± 0.029
6.92 ± 0.029
1.437 ± 0.018
2.201 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here