Bordetella phage FP1
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D0W9P6|A0A2D0W9P6_9CAUD Terminase large subunit OS=Bordetella phage FP1 OX=1916125 PE=4 SV=1
MM1 pKa = 6.96 EE2 pKa = 3.81 QRR4 pKa = 11.84 VYY6 pKa = 10.9 RR7 pKa = 11.84 DD8 pKa = 3.47 SAPIDD13 pKa = 4.17 PDD15 pKa = 3.56 NLPTPLEE22 pKa = 4.09 TLAPGVLSWTDD33 pKa = 3.3 EE34 pKa = 4.0 NATQGLTYY42 pKa = 10.02 YY43 pKa = 9.71 YY44 pKa = 10.46 AVSALVQGEE53 pKa = 4.35 EE54 pKa = 4.39 VVSDD58 pKa = 3.66 SLEE61 pKa = 4.17 VLVEE65 pKa = 4.18 PTWPAAPHH73 pKa = 5.07 WRR75 pKa = 11.84 VNITASHH82 pKa = 6.72 ASSGFQFWIAEE93 pKa = 4.12 MEE95 pKa = 4.11 LRR97 pKa = 11.84 EE98 pKa = 4.35 TPGGPSVATGGTPSASVQYY117 pKa = 10.73 AGNEE121 pKa = 3.61 AQYY124 pKa = 11.59 AFDD127 pKa = 4.75 GNPLTGWSSTSGANPPQWLRR147 pKa = 11.84 YY148 pKa = 8.8 SLPSGHH154 pKa = 6.85 PVAEE158 pKa = 4.38 VALQAGDD165 pKa = 3.31 TSARR169 pKa = 11.84 AGRR172 pKa = 11.84 APAALQIQYY181 pKa = 11.22 SLDD184 pKa = 3.19 GVNWITAKK192 pKa = 10.07 TYY194 pKa = 10.26 TGIPAWAAGEE204 pKa = 4.08 VRR206 pKa = 11.84 TFSTLL211 pKa = 3.12
Molecular weight: 22.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.251
IPC2_protein 4.444
IPC_protein 4.342
Toseland 4.19
ProMoST 4.444
Dawson 4.279
Bjellqvist 4.431
Wikipedia 4.151
Rodwell 4.177
Grimsley 4.101
Solomon 4.279
Lehninger 4.228
Nozaki 4.393
DTASelect 4.52
Thurlkill 4.19
EMBOSS 4.164
Sillero 4.444
Patrickios 2.88
IPC_peptide 4.279
IPC2_peptide 4.444
IPC2.peptide.svr19 4.36
Protein with the highest isoelectric point:
>tr|A0A2D0W9J5|A0A2D0W9J5_9CAUD Uncharacterized protein OS=Bordetella phage FP1 OX=1916125 PE=4 SV=1
MM1 pKa = 7.76 RR2 pKa = 11.84 ALILILAVLPLLAPQAHH19 pKa = 6.33 AEE21 pKa = 4.11 EE22 pKa = 4.34 VRR24 pKa = 11.84 PYY26 pKa = 10.45 SAQEE30 pKa = 3.88 TIRR33 pKa = 11.84 RR34 pKa = 11.84 AQVGTHH40 pKa = 5.64 SPRR43 pKa = 11.84 LAPPVRR49 pKa = 11.84 VAPTPARR56 pKa = 11.84 AARR59 pKa = 11.84 PSRR62 pKa = 11.84 YY63 pKa = 10.02 DD64 pKa = 3.37 RR65 pKa = 11.84 MVCRR69 pKa = 11.84 VDD71 pKa = 3.25 PAKK74 pKa = 10.2 PLHH77 pKa = 5.28 QQIICEE83 pKa = 4.1 KK84 pKa = 10.3 QKK86 pKa = 11.27
Molecular weight: 9.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.319
IPC2_protein 9.736
IPC_protein 10.862
Toseland 10.921
ProMoST 11.038
Dawson 10.994
Bjellqvist 10.818
Wikipedia 11.301
Rodwell 10.979
Grimsley 11.038
Solomon 11.257
Lehninger 11.199
Nozaki 10.921
DTASelect 10.818
Thurlkill 10.921
EMBOSS 11.359
Sillero 10.95
Patrickios 10.76
IPC_peptide 11.257
IPC2_peptide 10.189
IPC2.peptide.svr19 8.738
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
80
0
80
18739
37
938
234.2
25.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.833 ± 0.413
1.025 ± 0.089
6.1 ± 0.198
7.215 ± 0.209
3.618 ± 0.136
7.994 ± 0.208
1.804 ± 0.15
4.573 ± 0.171
4.317 ± 0.281
8.811 ± 0.229
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.06 ± 0.115
3.511 ± 0.171
5.304 ± 0.22
4.008 ± 0.139
7.044 ± 0.288
4.632 ± 0.241
5.417 ± 0.26
6.937 ± 0.236
2.028 ± 0.117
2.77 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here