Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma pneumoniae

Average proteome isoelectric point is 7.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 686 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P41204|RL16_MYCPN 50S ribosomal protein L16 OS=Mycoplasma pneumoniae (strain ATCC 29342 / M129) OX=272634 GN=rplP PE=3 SV=2
MM1 pKa = 8.28DD2 pKa = 4.51INKK5 pKa = 9.68PGWNQSDD12 pKa = 3.96QQATAYY18 pKa = 10.42DD19 pKa = 4.25PNQQQYY25 pKa = 9.74YY26 pKa = 9.97GDD28 pKa = 3.59GSTYY32 pKa = 10.44YY33 pKa = 10.98DD34 pKa = 4.02PDD36 pKa = 3.38QAVDD40 pKa = 3.85PNQAYY45 pKa = 10.16YY46 pKa = 9.83PDD48 pKa = 5.06PNTYY52 pKa = 9.85PDD54 pKa = 3.02AAAYY58 pKa = 9.46YY59 pKa = 10.51GYY61 pKa = 10.7GQDD64 pKa = 3.51GQAYY68 pKa = 7.49PQDD71 pKa = 3.95YY72 pKa = 10.84AQDD75 pKa = 4.0PNQAYY80 pKa = 9.86YY81 pKa = 11.06ADD83 pKa = 4.06PNAYY87 pKa = 9.52QDD89 pKa = 3.74PNAYY93 pKa = 8.03TDD95 pKa = 3.56PNAYY99 pKa = 9.83VDD101 pKa = 3.84PNAYY105 pKa = 9.55QDD107 pKa = 3.46PNAYY111 pKa = 9.92VDD113 pKa = 3.88PNNYY117 pKa = 8.3TDD119 pKa = 3.51PNAYY123 pKa = 9.75YY124 pKa = 10.62GYY126 pKa = 10.1GQDD129 pKa = 3.51GQAYY133 pKa = 7.49PQDD136 pKa = 3.95YY137 pKa = 10.84AQDD140 pKa = 4.0PNQAYY145 pKa = 9.86YY146 pKa = 11.06ADD148 pKa = 4.06PNAYY152 pKa = 9.52QDD154 pKa = 3.74PNAYY158 pKa = 8.12TDD160 pKa = 3.81PYY162 pKa = 10.31YY163 pKa = 10.33VTSTDD168 pKa = 3.03PNAYY172 pKa = 9.53YY173 pKa = 10.61GQVDD177 pKa = 3.96NVPALEE183 pKa = 4.56ASDD186 pKa = 3.96LAYY189 pKa = 10.17EE190 pKa = 4.23VTPQEE195 pKa = 3.84QAAEE199 pKa = 4.04QEE201 pKa = 4.62LFSEE205 pKa = 4.94PEE207 pKa = 3.9TKK209 pKa = 10.3VIRR212 pKa = 11.84EE213 pKa = 3.55IHH215 pKa = 5.98EE216 pKa = 4.35FPFEE220 pKa = 4.37KK221 pKa = 9.86IRR223 pKa = 11.84SYY225 pKa = 11.0FQTDD229 pKa = 3.44FDD231 pKa = 4.97SYY233 pKa = 11.54NSRR236 pKa = 11.84LTQLKK241 pKa = 10.41DD242 pKa = 3.33KK243 pKa = 10.5LDD245 pKa = 3.6NAIFSMRR252 pKa = 11.84KK253 pKa = 9.92AIDD256 pKa = 3.56TVKK259 pKa = 10.6EE260 pKa = 3.78NSANLQIMKK269 pKa = 10.51QNFEE273 pKa = 4.21RR274 pKa = 11.84QLKK277 pKa = 8.02EE278 pKa = 3.67QQTQRR283 pKa = 11.84LTSNTDD289 pKa = 2.94AEE291 pKa = 4.65KK292 pKa = 10.47IGAKK296 pKa = 9.68INQLEE301 pKa = 4.05EE302 pKa = 3.76RR303 pKa = 11.84MQRR306 pKa = 11.84LSRR309 pKa = 11.84TMEE312 pKa = 3.98SVEE315 pKa = 3.94WTKK318 pKa = 11.02KK319 pKa = 9.23EE320 pKa = 3.77PRR322 pKa = 11.84QEE324 pKa = 3.95QFDD327 pKa = 3.94PRR329 pKa = 11.84FVDD332 pKa = 3.54PRR334 pKa = 11.84NFNNYY339 pKa = 8.19VNNTDD344 pKa = 2.94TMMSMFEE351 pKa = 4.31KK352 pKa = 10.53VLMMNLLRR360 pKa = 11.84STTPVQPPVQYY371 pKa = 7.39FTPQPLTASPRR382 pKa = 11.84PVYY385 pKa = 9.98EE386 pKa = 4.1EE387 pKa = 5.06PISASFRR394 pKa = 11.84RR395 pKa = 11.84RR396 pKa = 11.84GYY398 pKa = 10.58RR399 pKa = 11.84GDD401 pKa = 3.52EE402 pKa = 4.35FYY404 pKa = 11.05EE405 pKa = 4.12

Molecular weight: 47.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P75413|Y368_MYCPN UPF0134 protein MPN_368 OS=Mycoplasma pneumoniae (strain ATCC 29342 / M129) OX=272634 GN=MPN_368 PE=3 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84IEE4 pKa = 4.24AANLAGSLWICSVVNHH20 pKa = 6.38GVQGVGVPSWVPDD33 pKa = 3.89PEE35 pKa = 5.15LEE37 pKa = 4.19GAVPKK42 pKa = 10.74SSALSWTCWLLLEE55 pKa = 4.69PRR57 pKa = 11.84LIGALARR64 pKa = 11.84LLVSSSIWPLSSEE77 pKa = 3.79SDD79 pKa = 3.65FFFTATCNALTLVSPDD95 pKa = 3.6EE96 pKa = 4.13PHH98 pKa = 6.61VGWIGQIQMWLKK110 pKa = 9.68NQWPQRR116 pKa = 11.84PGVFHH121 pKa = 7.02CSSRR125 pKa = 11.84CPPRR129 pKa = 11.84RR130 pKa = 11.84SSPSSQTLPRR140 pKa = 11.84WWKK143 pKa = 9.92YY144 pKa = 9.76FDD146 pKa = 3.49HH147 pKa = 6.83SRR149 pKa = 11.84FAAVVSPTPFATAHH163 pKa = 5.0STPRR167 pKa = 11.84CAARR171 pKa = 11.84VKK173 pKa = 10.41RR174 pKa = 11.84QTGRR178 pKa = 11.84DD179 pKa = 3.3WRR181 pKa = 11.84GLAPPRR187 pKa = 11.84RR188 pKa = 11.84GPCFWPRR195 pKa = 11.84FTVGVQPHH203 pKa = 6.43SNQSRR208 pKa = 11.84QRR210 pKa = 11.84GG211 pKa = 3.49

Molecular weight: 23.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

686

0

686

239873

37

1882

349.7

39.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.672 ± 0.08

0.75 ± 0.027

4.974 ± 0.072

5.681 ± 0.12

5.599 ± 0.088

5.522 ± 0.105

1.81 ± 0.043

6.627 ± 0.094

8.562 ± 0.103

10.35 ± 0.112

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.591 ± 0.034

6.209 ± 0.067

3.49 ± 0.076

5.372 ± 0.104

3.487 ± 0.057

6.462 ± 0.094

5.956 ± 0.073

6.479 ± 0.077

1.18 ± 0.037

3.228 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski