Amino acid dipepetide frequency for Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.59AlaAla: 4.59 ± 0.181
0.705AlaCys: 0.705 ± 0.056
2.789AlaAsp: 2.789 ± 0.098
3.373AlaGlu: 3.373 ± 0.195
3.46AlaPhe: 3.46 ± 0.13
3.393AlaGly: 3.393 ± 0.14
1.113AlaHis: 1.113 ± 0.067
5.049AlaIle: 5.049 ± 0.17
5.666AlaLys: 5.666 ± 0.144
7.662AlaLeu: 7.662 ± 0.205
1.013AlaMet: 1.013 ± 0.063
4.261AlaAsn: 4.261 ± 0.154
2.264AlaPro: 2.264 ± 0.148
3.664AlaGln: 3.664 ± 0.135
2.197AlaArg: 2.197 ± 0.094
4.311AlaSer: 4.311 ± 0.136
3.819AlaThr: 3.819 ± 0.132
4.465AlaVal: 4.465 ± 0.145
0.65AlaTrp: 0.65 ± 0.046
2.276AlaTyr: 2.276 ± 0.123
0.0AlaXaa: 0.0 ± 0.0
Cys
0.442CysAla: 0.442 ± 0.045
0.117CysCys: 0.117 ± 0.021
0.509CysAsp: 0.509 ± 0.046
0.404CysGlu: 0.404 ± 0.045
0.604CysPhe: 0.604 ± 0.056
0.667CysGly: 0.667 ± 0.054
0.171CysHis: 0.171 ± 0.026
0.363CysIle: 0.363 ± 0.047
0.504CysLys: 0.504 ± 0.054
0.809CysLeu: 0.809 ± 0.064
0.108CysMet: 0.108 ± 0.024
0.288CysAsn: 0.288 ± 0.034
0.3CysPro: 0.3 ± 0.035
0.388CysGln: 0.388 ± 0.045
0.217CysArg: 0.217 ± 0.033
0.45CysSer: 0.45 ± 0.054
0.338CysThr: 0.338 ± 0.039
0.454CysVal: 0.454 ± 0.042
0.121CysTrp: 0.121 ± 0.024
0.242CysTyr: 0.242 ± 0.035
0.0CysXaa: 0.0 ± 0.0
Asp
2.831AspAla: 2.831 ± 0.102
0.396AspCys: 0.396 ± 0.042
2.139AspAsp: 2.139 ± 0.117
2.943AspGlu: 2.943 ± 0.127
3.594AspPhe: 3.594 ± 0.119
2.906AspGly: 2.906 ± 0.128
0.934AspHis: 0.934 ± 0.066
2.939AspIle: 2.939 ± 0.125
4.582AspLys: 4.582 ± 0.183
5.103AspLeu: 5.103 ± 0.167
0.684AspMet: 0.684 ± 0.049
2.806AspAsn: 2.806 ± 0.122
2.251AspPro: 2.251 ± 0.116
2.893AspGln: 2.893 ± 0.135
1.434AspArg: 1.434 ± 0.078
3.214AspSer: 3.214 ± 0.137
2.526AspThr: 2.526 ± 0.109
2.968AspVal: 2.968 ± 0.124
0.696AspTrp: 0.696 ± 0.056
1.905AspTyr: 1.905 ± 0.082
0.0AspXaa: 0.0 ± 0.0
Glu
3.84GluAla: 3.84 ± 0.156
0.271GluCys: 0.271 ± 0.038
1.972GluAsp: 1.972 ± 0.092
3.043GluGlu: 3.043 ± 0.149
2.506GluPhe: 2.506 ± 0.108
2.326GluGly: 2.326 ± 0.109
1.113GluHis: 1.113 ± 0.077
4.223GluIle: 4.223 ± 0.171
5.286GluLys: 5.286 ± 0.211
6.841GluLeu: 6.841 ± 0.295
1.071GluMet: 1.071 ± 0.066
3.331GluAsn: 3.331 ± 0.133
1.747GluPro: 1.747 ± 0.158
4.227GluGln: 4.227 ± 0.165
2.485GluArg: 2.485 ± 0.129
3.139GluSer: 3.139 ± 0.137
3.156GluThr: 3.156 ± 0.146
3.781GluVal: 3.781 ± 0.133
0.617GluTrp: 0.617 ± 0.051
1.834GluTyr: 1.834 ± 0.101
0.0GluXaa: 0.0 ± 0.0
Phe
3.719PheAla: 3.719 ± 0.12
0.546PheCys: 0.546 ± 0.052
3.619PheAsp: 3.619 ± 0.111
3.148PheGlu: 3.148 ± 0.128
3.227PhePhe: 3.227 ± 0.147
3.498PheGly: 3.498 ± 0.132
0.988PheHis: 0.988 ± 0.069
3.302PheIle: 3.302 ± 0.129
4.652PheLys: 4.652 ± 0.136
5.586PheLeu: 5.586 ± 0.195
0.671PheMet: 0.671 ± 0.049
3.844PheAsn: 3.844 ± 0.137
1.513PhePro: 1.513 ± 0.086
2.343PheGln: 2.343 ± 0.103
1.78PheArg: 1.78 ± 0.097
4.181PheSer: 4.181 ± 0.164
2.877PheThr: 2.877 ± 0.115
3.915PheVal: 3.915 ± 0.145
0.705PheTrp: 0.705 ± 0.061
1.876PheTyr: 1.876 ± 0.087
0.0PheXaa: 0.0 ± 0.0
Gly
3.189GlyAla: 3.189 ± 0.137
0.375GlyCys: 0.375 ± 0.038
2.606GlyAsp: 2.606 ± 0.118
2.881GlyGlu: 2.881 ± 0.126
3.348GlyPhe: 3.348 ± 0.115
3.719GlyGly: 3.719 ± 0.176
0.884GlyHis: 0.884 ± 0.068
4.369GlyIle: 4.369 ± 0.176
4.59GlyLys: 4.59 ± 0.156
5.595GlyLeu: 5.595 ± 0.195
1.063GlyMet: 1.063 ± 0.077
2.977GlyAsn: 2.977 ± 0.129
1.163GlyPro: 1.163 ± 0.077
2.372GlyGln: 2.372 ± 0.118
1.818GlyArg: 1.818 ± 0.101
4.273GlySer: 4.273 ± 0.189
3.081GlyThr: 3.081 ± 0.139
3.91GlyVal: 3.91 ± 0.133
0.775GlyTrp: 0.775 ± 0.07
2.23GlyTyr: 2.23 ± 0.113
0.0GlyXaa: 0.0 ± 0.0
His
0.984HisAla: 0.984 ± 0.068
0.188HisCys: 0.188 ± 0.03
0.925HisAsp: 0.925 ± 0.059
0.917HisGlu: 0.917 ± 0.067
1.426HisPhe: 1.426 ± 0.077
0.988HisGly: 0.988 ± 0.064
0.517HisHis: 0.517 ± 0.047
0.875HisIle: 0.875 ± 0.068
1.488HisLys: 1.488 ± 0.079
2.039HisLeu: 2.039 ± 0.107
0.279HisMet: 0.279 ± 0.036
1.267HisAsn: 1.267 ± 0.071
0.755HisPro: 0.755 ± 0.056
1.021HisGln: 1.021 ± 0.077
0.588HisArg: 0.588 ± 0.054
1.013HisSer: 1.013 ± 0.064
0.955HisThr: 0.955 ± 0.067
0.892HisVal: 0.892 ± 0.063
0.163HisTrp: 0.163 ± 0.026
0.821HisTyr: 0.821 ± 0.06
0.0HisXaa: 0.0 ± 0.0
Ile
5.253IleAla: 5.253 ± 0.173
0.596IleCys: 0.596 ± 0.052
3.794IleAsp: 3.794 ± 0.14
4.073IleGlu: 4.073 ± 0.142
3.56IlePhe: 3.56 ± 0.129
3.481IleGly: 3.481 ± 0.131
1.163IleHis: 1.163 ± 0.065
4.586IleIle: 4.586 ± 0.167
6.166IleLys: 6.166 ± 0.183
5.457IleLeu: 5.457 ± 0.165
0.925IleMet: 0.925 ± 0.068
4.657IleAsn: 4.657 ± 0.166
2.776IlePro: 2.776 ± 0.117
2.526IleGln: 2.526 ± 0.097
2.164IleArg: 2.164 ± 0.096
3.994IleSer: 3.994 ± 0.136
3.873IleThr: 3.873 ± 0.143
4.552IleVal: 4.552 ± 0.138
0.621IleTrp: 0.621 ± 0.054
2.051IleTyr: 2.051 ± 0.112
0.004IleXaa: 0.004 ± 0.004
Lys
5.978LysAla: 5.978 ± 0.154
0.342LysCys: 0.342 ± 0.044
4.582LysAsp: 4.582 ± 0.168
5.453LysGlu: 5.453 ± 0.188
3.077LysPhe: 3.077 ± 0.108
3.931LysGly: 3.931 ± 0.151
1.901LysHis: 1.901 ± 0.096
5.278LysIle: 5.278 ± 0.17
7.4LysLys: 7.4 ± 0.227
8.575LysLeu: 8.575 ± 0.228
1.651LysMet: 1.651 ± 0.103
5.132LysAsn: 5.132 ± 0.154
3.135LysPro: 3.135 ± 0.126
6.178LysGln: 6.178 ± 0.248
3.748LysArg: 3.748 ± 0.141
4.998LysSer: 4.998 ± 0.17
5.52LysThr: 5.52 ± 0.157
5.249LysVal: 5.249 ± 0.16
0.896LysTrp: 0.896 ± 0.074
2.597LysTyr: 2.597 ± 0.102
0.0LysXaa: 0.0 ± 0.0
Leu
7.283LeuAla: 7.283 ± 0.179
0.771LeuCys: 0.771 ± 0.054
5.453LeuAsp: 5.453 ± 0.141
5.945LeuGlu: 5.945 ± 0.235
5.741LeuPhe: 5.741 ± 0.176
5.486LeuGly: 5.486 ± 0.179
1.863LeuHis: 1.863 ± 0.09
7.471LeuIle: 7.471 ± 0.199
9.588LeuLys: 9.588 ± 0.245
10.189LeuLeu: 10.189 ± 0.273
1.768LeuMet: 1.768 ± 0.099
6.558LeuAsn: 6.558 ± 0.165
3.498LeuPro: 3.498 ± 0.135
4.99LeuGln: 4.99 ± 0.202
3.298LeuArg: 3.298 ± 0.126
6.724LeuSer: 6.724 ± 0.194
6.162LeuThr: 6.162 ± 0.175
7.07LeuVal: 7.07 ± 0.2
1.067LeuTrp: 1.067 ± 0.074
2.572LeuTyr: 2.572 ± 0.098
0.0LeuXaa: 0.0 ± 0.0
Met
1.272MetAla: 1.272 ± 0.08
0.117MetCys: 0.117 ± 0.024
0.771MetAsp: 0.771 ± 0.061
1.188MetGlu: 1.188 ± 0.076
0.675MetPhe: 0.675 ± 0.054
0.971MetGly: 0.971 ± 0.079
0.25MetHis: 0.25 ± 0.032
1.034MetIle: 1.034 ± 0.074
1.326MetLys: 1.326 ± 0.08
1.559MetLeu: 1.559 ± 0.078
0.35MetMet: 0.35 ± 0.043
0.788MetAsn: 0.788 ± 0.061
0.546MetPro: 0.546 ± 0.049
0.78MetGln: 0.78 ± 0.06
0.721MetArg: 0.721 ± 0.054
0.984MetSer: 0.984 ± 0.067
0.834MetThr: 0.834 ± 0.062
1.301MetVal: 1.301 ± 0.069
0.1MetTrp: 0.1 ± 0.019
0.342MetTyr: 0.342 ± 0.042
0.0MetXaa: 0.0 ± 0.0
Asn
3.402AsnAla: 3.402 ± 0.114
0.484AsnCys: 0.484 ± 0.045
2.747AsnAsp: 2.747 ± 0.108
3.623AsnGlu: 3.623 ± 0.135
3.806AsnPhe: 3.806 ± 0.135
3.735AsnGly: 3.735 ± 0.169
1.188AsnHis: 1.188 ± 0.073
3.489AsnIle: 3.489 ± 0.135
5.286AsnLys: 5.286 ± 0.158
6.466AsnLeu: 6.466 ± 0.17
0.842AsnMet: 0.842 ± 0.057
4.311AsnAsn: 4.311 ± 0.154
2.776AsnPro: 2.776 ± 0.116
3.99AsnGln: 3.99 ± 0.152
2.059AsnArg: 2.059 ± 0.096
3.935AsnSer: 3.935 ± 0.161
3.714AsnThr: 3.714 ± 0.138
3.031AsnVal: 3.031 ± 0.109
0.98AsnTrp: 0.98 ± 0.073
2.222AsnTyr: 2.222 ± 0.096
0.0AsnXaa: 0.0 ± 0.0
Pro
2.226ProAla: 2.226 ± 0.112
0.183ProCys: 0.183 ± 0.029
1.622ProAsp: 1.622 ± 0.094
2.134ProGlu: 2.134 ± 0.123
1.763ProPhe: 1.763 ± 0.094
1.668ProGly: 1.668 ± 0.105
0.705ProHis: 0.705 ± 0.061
2.039ProIle: 2.039 ± 0.097
2.877ProLys: 2.877 ± 0.124
3.585ProLeu: 3.585 ± 0.125
0.404ProMet: 0.404 ± 0.046
2.46ProAsn: 2.46 ± 0.106
1.171ProPro: 1.171 ± 0.101
1.909ProGln: 1.909 ± 0.102
1.259ProArg: 1.259 ± 0.083
1.918ProSer: 1.918 ± 0.08
2.43ProThr: 2.43 ± 0.117
2.881ProVal: 2.881 ± 0.143
0.488ProTrp: 0.488 ± 0.05
1.176ProTyr: 1.176 ± 0.085
0.0ProXaa: 0.0 ± 0.0
Gln
4.073GlnAla: 4.073 ± 0.147
0.2GlnCys: 0.2 ± 0.033
2.155GlnAsp: 2.155 ± 0.119
2.43GlnGlu: 2.43 ± 0.122
2.693GlnPhe: 2.693 ± 0.115
2.576GlnGly: 2.576 ± 0.121
0.813GlnHis: 0.813 ± 0.066
4.019GlnIle: 4.019 ± 0.162
4.886GlnLys: 4.886 ± 0.218
6.349GlnLeu: 6.349 ± 0.222
0.992GlnMet: 0.992 ± 0.068
2.964GlnAsn: 2.964 ± 0.139
1.943GlnPro: 1.943 ± 0.15
3.514GlnGln: 3.514 ± 0.18
2.276GlnArg: 2.276 ± 0.102
3.031GlnSer: 3.031 ± 0.127
3.785GlnThr: 3.785 ± 0.142
2.956GlnVal: 2.956 ± 0.119
0.521GlnTrp: 0.521 ± 0.04
1.538GlnTyr: 1.538 ± 0.088
0.0GlnXaa: 0.0 ± 0.0
Arg
2.326ArgAla: 2.326 ± 0.12
0.325ArgCys: 0.325 ± 0.042
1.826ArgAsp: 1.826 ± 0.102
2.08ArgGlu: 2.08 ± 0.12
2.36ArgPhe: 2.36 ± 0.096
1.83ArgGly: 1.83 ± 0.083
0.567ArgHis: 0.567 ± 0.051
2.485ArgIle: 2.485 ± 0.102
2.714ArgLys: 2.714 ± 0.113
3.76ArgLeu: 3.76 ± 0.147
0.667ArgMet: 0.667 ± 0.057
1.976ArgAsn: 1.976 ± 0.09
1.042ArgPro: 1.042 ± 0.077
1.801ArgGln: 1.801 ± 0.094
1.309ArgArg: 1.309 ± 0.092
1.934ArgSer: 1.934 ± 0.092
1.855ArgThr: 1.855 ± 0.11
2.268ArgVal: 2.268 ± 0.093
0.488ArgTrp: 0.488 ± 0.05
1.259ArgTyr: 1.259 ± 0.067
0.0ArgXaa: 0.0 ± 0.0
Ser
4.373SerAla: 4.373 ± 0.182
0.567SerCys: 0.567 ± 0.049
3.239SerAsp: 3.239 ± 0.157
3.381SerGlu: 3.381 ± 0.132
4.694SerPhe: 4.694 ± 0.156
4.594SerGly: 4.594 ± 0.213
0.888SerHis: 0.888 ± 0.061
3.765SerIle: 3.765 ± 0.131
4.648SerLys: 4.648 ± 0.17
6.699SerLeu: 6.699 ± 0.165
1.013SerMet: 1.013 ± 0.072
3.923SerAsn: 3.923 ± 0.14
1.872SerPro: 1.872 ± 0.091
2.881SerGln: 2.881 ± 0.122
1.834SerArg: 1.834 ± 0.087
5.386SerSer: 5.386 ± 0.285
4.161SerThr: 4.161 ± 0.189
3.539SerVal: 3.539 ± 0.121
0.875SerTrp: 0.875 ± 0.055
2.289SerTyr: 2.289 ± 0.094
0.0SerXaa: 0.0 ± 0.0
Thr
4.119ThrAla: 4.119 ± 0.152
0.258ThrCys: 0.258 ± 0.035
3.002ThrAsp: 3.002 ± 0.13
3.139ThrGlu: 3.139 ± 0.131
2.706ThrPhe: 2.706 ± 0.117
3.264ThrGly: 3.264 ± 0.148
1.088ThrHis: 1.088 ± 0.082
3.885ThrIle: 3.885 ± 0.135
5.153ThrLys: 5.153 ± 0.164
5.495ThrLeu: 5.495 ± 0.157
0.755ThrMet: 0.755 ± 0.046
3.927ThrAsn: 3.927 ± 0.148
2.664ThrPro: 2.664 ± 0.134
3.127ThrGln: 3.127 ± 0.116
1.951ThrArg: 1.951 ± 0.095
3.981ThrSer: 3.981 ± 0.208
4.882ThrThr: 4.882 ± 0.192
4.048ThrVal: 4.048 ± 0.151
0.638ThrTrp: 0.638 ± 0.054
1.48ThrTyr: 1.48 ± 0.072
0.0ThrXaa: 0.0 ± 0.0
Val
4.194ValAla: 4.194 ± 0.151
0.613ValCys: 0.613 ± 0.058
3.739ValAsp: 3.739 ± 0.143
4.273ValGlu: 4.273 ± 0.2
3.61ValPhe: 3.61 ± 0.138
3.644ValGly: 3.644 ± 0.143
1.142ValHis: 1.142 ± 0.073
4.594ValIle: 4.594 ± 0.142
5.274ValLys: 5.274 ± 0.153
6.537ValLeu: 6.537 ± 0.179
1.096ValMet: 1.096 ± 0.07
4.106ValAsn: 4.106 ± 0.124
2.043ValPro: 2.043 ± 0.116
2.993ValGln: 2.993 ± 0.122
1.972ValArg: 1.972 ± 0.09
4.348ValSer: 4.348 ± 0.145
3.102ValThr: 3.102 ± 0.107
4.865ValVal: 4.865 ± 0.176
0.688ValTrp: 0.688 ± 0.055
1.955ValTyr: 1.955 ± 0.091
0.0ValXaa: 0.0 ± 0.0
Trp
0.742TrpAla: 0.742 ± 0.066
0.063TrpCys: 0.063 ± 0.015
0.409TrpAsp: 0.409 ± 0.044
0.484TrpGlu: 0.484 ± 0.052
0.825TrpPhe: 0.825 ± 0.07
0.5TrpGly: 0.5 ± 0.053
0.163TrpHis: 0.163 ± 0.027
0.767TrpIle: 0.767 ± 0.053
1.192TrpLys: 1.192 ± 0.069
1.447TrpLeu: 1.447 ± 0.086
0.213TrpMet: 0.213 ± 0.027
0.817TrpAsn: 0.817 ± 0.065
0.35TrpPro: 0.35 ± 0.039
0.384TrpGln: 0.384 ± 0.041
0.471TrpArg: 0.471 ± 0.042
0.813TrpSer: 0.813 ± 0.067
0.813TrpThr: 0.813 ± 0.072
0.684TrpVal: 0.684 ± 0.055
0.238TrpTrp: 0.238 ± 0.032
0.429TrpTyr: 0.429 ± 0.048
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.884TyrAla: 1.884 ± 0.084
0.379TyrCys: 0.379 ± 0.04
1.847TyrAsp: 1.847 ± 0.092
1.897TyrGlu: 1.897 ± 0.105
2.31TyrPhe: 2.31 ± 0.109
2.039TyrGly: 2.039 ± 0.101
0.65TyrHis: 0.65 ± 0.054
1.743TyrIle: 1.743 ± 0.086
2.347TyrLys: 2.347 ± 0.109
3.744TyrLeu: 3.744 ± 0.123
0.338TyrMet: 0.338 ± 0.039
1.697TyrAsn: 1.697 ± 0.07
1.088TyrPro: 1.088 ± 0.073
1.734TyrGln: 1.734 ± 0.089
1.292TyrArg: 1.292 ± 0.076
1.993TyrSer: 1.993 ± 0.093
1.68TyrThr: 1.68 ± 0.077
1.959TyrVal: 1.959 ± 0.081
0.475TyrTrp: 0.475 ± 0.046
1.18TyrTyr: 1.18 ± 0.079
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.004XaaPhe: 0.004 ± 0.004
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 686 proteins (239873 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski