Erythrobacter litoralis (strain HTCC2594)
Average proteome isoelectric point is 5.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2999 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2N5Y4|Q2N5Y4_ERYLH Serine palmitoyltransferase OS=Erythrobacter litoralis (strain HTCC2594) OX=314225 GN=ELI_14075 PE=3 SV=1
MM1 pKa = 7.18 ATADD5 pKa = 4.47 LEE7 pKa = 4.39 YY8 pKa = 10.57 LYY10 pKa = 11.04 AALLQDD16 pKa = 3.66 MVSADD21 pKa = 4.1 EE22 pKa = 4.06 QSLALTEE29 pKa = 4.16 RR30 pKa = 11.84 LRR32 pKa = 11.84 DD33 pKa = 3.39 AATSEE38 pKa = 4.21 DD39 pKa = 4.14 LRR41 pKa = 11.84 DD42 pKa = 3.68 ALEE45 pKa = 5.24 DD46 pKa = 4.14 GVCGIGDD53 pKa = 3.76 GLEE56 pKa = 4.08 IARR59 pKa = 11.84 KK60 pKa = 8.87 LAEE63 pKa = 4.31 AHH65 pKa = 6.6 PADD68 pKa = 4.73 DD69 pKa = 6.45 GEE71 pKa = 4.3 FQSDD75 pKa = 3.3 GMAGLVTDD83 pKa = 3.45 TQEE86 pKa = 4.41 RR87 pKa = 11.84 VFEE90 pKa = 4.17 TDD92 pKa = 3.38 FASGSARR99 pKa = 11.84 DD100 pKa = 3.72 AAILAQYY107 pKa = 8.35 MHH109 pKa = 6.81 LTYY112 pKa = 10.68 YY113 pKa = 11.0 GLAGYY118 pKa = 7.61 RR119 pKa = 11.84 TLAAIASQLGHH130 pKa = 7.07 ADD132 pKa = 3.53 EE133 pKa = 6.58 AEE135 pKa = 4.17 DD136 pKa = 5.14 LQTCYY141 pKa = 10.93 EE142 pKa = 4.07 NAQDD146 pKa = 4.77 GQDD149 pKa = 4.2 DD150 pKa = 4.67 MIGLLDD156 pKa = 4.04 GEE158 pKa = 4.61 VLEE161 pKa = 4.93 DD162 pKa = 3.54 TAA164 pKa = 5.9
Molecular weight: 17.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.706
IPC_protein 3.706
Toseland 3.49
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.643
Sillero 3.834
Patrickios 1.85
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|Q2NC79|Q2NC79_ERYLH Biotin carboxylase OS=Erythrobacter litoralis (strain HTCC2594) OX=314225 GN=ELI_03100 PE=4 SV=1
MM1 pKa = 7.1 KK2 pKa = 10.16 HH3 pKa = 6.16 RR4 pKa = 11.84 RR5 pKa = 11.84 QTLLMILMRR14 pKa = 11.84 FSIRR18 pKa = 11.84 IWFVLSRR25 pKa = 11.84 WLISLNRR32 pKa = 11.84 RR33 pKa = 11.84 HH34 pKa = 6.16
Molecular weight: 4.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.465
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.106
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.135
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2999
0
2999
928556
23
2409
309.6
33.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.305 ± 0.071
0.812 ± 0.014
6.349 ± 0.038
6.605 ± 0.047
3.699 ± 0.031
8.802 ± 0.044
1.915 ± 0.023
5.088 ± 0.029
3.326 ± 0.043
9.627 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.488 ± 0.028
2.628 ± 0.031
5.083 ± 0.035
3.222 ± 0.024
6.888 ± 0.04
5.341 ± 0.037
5.232 ± 0.044
6.943 ± 0.04
1.432 ± 0.02
2.216 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here