Escherichia phage ECBP5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Gajwadongvirus; Escherichia virus ECBP5

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6N5M8|A0A0F6N5M8_9CAUD Uncharacterized protein OS=Escherichia phage ECBP5 OX=1498172 GN=ECBP5_0058 PE=4 SV=1
MM1 pKa = 7.41EE2 pKa = 4.71VSCSSWCQLKK12 pKa = 8.59WQEE15 pKa = 4.15AVEE18 pKa = 4.57SGDD21 pKa = 4.31DD22 pKa = 3.75LAASDD27 pKa = 4.1YY28 pKa = 11.42LKK30 pKa = 10.75LYY32 pKa = 8.42EE33 pKa = 4.26LWSSRR38 pKa = 11.84GMM40 pKa = 3.65

Molecular weight: 4.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6N5N0|A0A0F6N5N0_9CAUD Uncharacterized protein OS=Escherichia phage ECBP5 OX=1498172 GN=ECBP5_0003 PE=4 SV=1
MM1 pKa = 7.92SYY3 pKa = 10.15PISYY7 pKa = 9.1QWCSKK12 pKa = 10.26LKK14 pKa = 9.68TVAHH18 pKa = 4.66QTKK21 pKa = 10.36DD22 pKa = 2.52RR23 pKa = 11.84YY24 pKa = 6.93TQQRR28 pKa = 11.84TSTRR32 pKa = 11.84VQQATALSSSQSASSHH48 pKa = 3.79WQANRR53 pKa = 11.84SHH55 pKa = 6.65SGAEE59 pKa = 3.79AHH61 pKa = 6.5SGSLAAHH68 pKa = 5.98NRR70 pKa = 11.84LSVSHH75 pKa = 6.89LASSQQLPDD84 pKa = 3.51QPLVDD89 pKa = 3.58QSVV92 pKa = 2.77

Molecular weight: 10.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13919

38

1287

224.5

25.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.923 ± 0.423

1.07 ± 0.143

6.25 ± 0.245

6.617 ± 0.352

3.829 ± 0.191

7.091 ± 0.262

1.94 ± 0.19

4.893 ± 0.203

6.61 ± 0.282

8.391 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.125 ± 0.169

4.347 ± 0.276

3.657 ± 0.166

4.684 ± 0.31

4.612 ± 0.219

6.071 ± 0.3

5.862 ± 0.257

6.875 ± 0.302

1.394 ± 0.128

3.757 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski