Amino acid dipepetide frequency for Escherichia phage ECBP5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.987AlaAla: 9.987 ± 1.445
0.287AlaCys: 0.287 ± 0.169
4.67AlaAsp: 4.67 ± 0.558
5.964AlaGlu: 5.964 ± 0.562
3.449AlaPhe: 3.449 ± 0.497
6.826AlaGly: 6.826 ± 0.867
1.724AlaHis: 1.724 ± 0.382
5.317AlaIle: 5.317 ± 0.52
6.395AlaLys: 6.395 ± 0.86
5.532AlaLeu: 5.532 ± 0.639
2.874AlaMet: 2.874 ± 0.415
4.455AlaAsn: 4.455 ± 0.509
2.371AlaPro: 2.371 ± 0.484
5.029AlaGln: 5.029 ± 0.865
3.305AlaArg: 3.305 ± 0.598
4.67AlaSer: 4.67 ± 0.506
5.389AlaThr: 5.389 ± 0.542
6.323AlaVal: 6.323 ± 0.754
1.509AlaTrp: 1.509 ± 0.312
3.161AlaTyr: 3.161 ± 0.442
0.0AlaXaa: 0.0 ± 0.0
Cys
0.503CysAla: 0.503 ± 0.17
0.144CysCys: 0.144 ± 0.098
0.575CysAsp: 0.575 ± 0.222
0.359CysGlu: 0.359 ± 0.158
0.575CysPhe: 0.575 ± 0.278
1.006CysGly: 1.006 ± 0.322
0.359CysHis: 0.359 ± 0.157
0.647CysIle: 0.647 ± 0.217
0.647CysLys: 0.647 ± 0.19
0.934CysLeu: 0.934 ± 0.294
0.431CysMet: 0.431 ± 0.193
0.144CysAsn: 0.144 ± 0.089
0.575CysPro: 0.575 ± 0.213
0.503CysGln: 0.503 ± 0.247
0.575CysArg: 0.575 ± 0.196
0.934CysSer: 0.934 ± 0.277
0.647CysThr: 0.647 ± 0.229
0.431CysVal: 0.431 ± 0.167
0.144CysTrp: 0.144 ± 0.105
0.575CysTyr: 0.575 ± 0.281
0.0CysXaa: 0.0 ± 0.0
Asp
5.748AspAla: 5.748 ± 0.599
0.862AspCys: 0.862 ± 0.276
4.239AspAsp: 4.239 ± 0.606
4.455AspGlu: 4.455 ± 0.473
2.587AspPhe: 2.587 ± 0.374
5.245AspGly: 5.245 ± 0.703
0.718AspHis: 0.718 ± 0.259
3.018AspIle: 3.018 ± 0.415
3.305AspLys: 3.305 ± 0.324
5.532AspLeu: 5.532 ± 0.48
1.724AspMet: 1.724 ± 0.349
2.802AspAsn: 2.802 ± 0.493
2.299AspPro: 2.299 ± 0.407
1.653AspGln: 1.653 ± 0.377
2.371AspArg: 2.371 ± 0.54
3.952AspSer: 3.952 ± 0.576
3.664AspThr: 3.664 ± 0.49
3.952AspVal: 3.952 ± 0.414
1.078AspTrp: 1.078 ± 0.337
3.305AspTyr: 3.305 ± 0.453
0.0AspXaa: 0.0 ± 0.0
Glu
6.969GluAla: 6.969 ± 0.985
0.575GluCys: 0.575 ± 0.213
4.311GluAsp: 4.311 ± 0.547
4.527GluGlu: 4.527 ± 0.637
2.155GluPhe: 2.155 ± 0.455
3.808GluGly: 3.808 ± 0.48
1.293GluHis: 1.293 ± 0.333
2.371GluIle: 2.371 ± 0.451
4.095GluLys: 4.095 ± 0.724
7.544GluLeu: 7.544 ± 0.77
2.012GluMet: 2.012 ± 0.401
1.796GluAsn: 1.796 ± 0.343
1.94GluPro: 1.94 ± 0.345
4.527GluGln: 4.527 ± 0.548
2.946GluArg: 2.946 ± 0.506
3.592GluSer: 3.592 ± 0.365
2.73GluThr: 2.73 ± 0.557
5.029GluVal: 5.029 ± 0.58
1.509GluTrp: 1.509 ± 0.383
2.443GluTyr: 2.443 ± 0.363
0.0GluXaa: 0.0 ± 0.0
Phe
2.515PheAla: 2.515 ± 0.435
0.575PheCys: 0.575 ± 0.2
2.874PheAsp: 2.874 ± 0.425
2.227PheGlu: 2.227 ± 0.4
1.509PhePhe: 1.509 ± 0.296
3.161PheGly: 3.161 ± 0.545
0.718PheHis: 0.718 ± 0.25
2.299PheIle: 2.299 ± 0.31
2.946PheLys: 2.946 ± 0.408
2.874PheLeu: 2.874 ± 0.425
1.365PheMet: 1.365 ± 0.333
2.946PheAsn: 2.946 ± 0.388
1.006PhePro: 1.006 ± 0.304
1.221PheGln: 1.221 ± 0.245
1.94PheArg: 1.94 ± 0.37
2.012PheSer: 2.012 ± 0.351
2.227PheThr: 2.227 ± 0.43
2.227PheVal: 2.227 ± 0.343
0.287PheTrp: 0.287 ± 0.134
1.365PheTyr: 1.365 ± 0.432
0.0PheXaa: 0.0 ± 0.0
Gly
5.029GlyAla: 5.029 ± 0.733
1.15GlyCys: 1.15 ± 0.309
5.676GlyAsp: 5.676 ± 0.666
4.814GlyGlu: 4.814 ± 0.635
3.233GlyPhe: 3.233 ± 0.432
4.742GlyGly: 4.742 ± 0.671
1.365GlyHis: 1.365 ± 0.4
3.736GlyIle: 3.736 ± 0.598
5.964GlyLys: 5.964 ± 0.839
6.035GlyLeu: 6.035 ± 0.731
2.227GlyMet: 2.227 ± 0.443
3.305GlyAsn: 3.305 ± 0.744
0.144GlyPro: 0.144 ± 0.091
2.371GlyGln: 2.371 ± 0.477
3.521GlyArg: 3.521 ± 0.549
3.736GlySer: 3.736 ± 0.587
4.67GlyThr: 4.67 ± 0.595
5.245GlyVal: 5.245 ± 0.53
0.934GlyTrp: 0.934 ± 0.179
3.018GlyTyr: 3.018 ± 0.461
0.0GlyXaa: 0.0 ± 0.0
His
0.862HisAla: 0.862 ± 0.219
0.287HisCys: 0.287 ± 0.144
1.724HisAsp: 1.724 ± 0.358
1.15HisGlu: 1.15 ± 0.295
0.79HisPhe: 0.79 ± 0.345
1.437HisGly: 1.437 ± 0.278
0.503HisHis: 0.503 ± 0.3
0.718HisIle: 0.718 ± 0.211
1.437HisLys: 1.437 ± 0.387
2.155HisLeu: 2.155 ± 0.467
0.431HisMet: 0.431 ± 0.166
0.718HisAsn: 0.718 ± 0.255
0.575HisPro: 0.575 ± 0.259
1.006HisGln: 1.006 ± 0.246
1.006HisArg: 1.006 ± 0.332
0.934HisSer: 0.934 ± 0.316
1.365HisThr: 1.365 ± 0.352
1.15HisVal: 1.15 ± 0.365
0.359HisTrp: 0.359 ± 0.155
0.79HisTyr: 0.79 ± 0.268
0.0HisXaa: 0.0 ± 0.0
Ile
5.101IleAla: 5.101 ± 0.654
0.359IleCys: 0.359 ± 0.215
3.018IleAsp: 3.018 ± 0.47
2.658IleGlu: 2.658 ± 0.403
1.581IlePhe: 1.581 ± 0.351
3.018IleGly: 3.018 ± 0.45
1.796IleHis: 1.796 ± 0.417
2.371IleIle: 2.371 ± 0.431
3.664IleLys: 3.664 ± 0.487
3.305IleLeu: 3.305 ± 0.479
1.365IleMet: 1.365 ± 0.258
2.515IleAsn: 2.515 ± 0.424
2.227IlePro: 2.227 ± 0.446
1.581IleGln: 1.581 ± 0.372
2.587IleArg: 2.587 ± 0.427
3.592IleSer: 3.592 ± 0.604
3.88IleThr: 3.88 ± 0.539
2.73IleVal: 2.73 ± 0.469
0.359IleTrp: 0.359 ± 0.162
1.221IleTyr: 1.221 ± 0.304
0.0IleXaa: 0.0 ± 0.0
Lys
6.538LysAla: 6.538 ± 0.872
0.718LysCys: 0.718 ± 0.25
4.383LysAsp: 4.383 ± 0.542
4.239LysGlu: 4.239 ± 0.49
2.084LysPhe: 2.084 ± 0.367
4.455LysGly: 4.455 ± 0.711
0.934LysHis: 0.934 ± 0.286
2.227LysIle: 2.227 ± 0.401
3.521LysLys: 3.521 ± 0.539
6.969LysLeu: 6.969 ± 0.79
2.371LysMet: 2.371 ± 0.47
2.084LysAsn: 2.084 ± 0.408
3.233LysPro: 3.233 ± 0.46
3.521LysGln: 3.521 ± 0.484
3.521LysArg: 3.521 ± 0.473
4.024LysSer: 4.024 ± 0.602
2.658LysThr: 2.658 ± 0.315
5.029LysVal: 5.029 ± 0.637
1.221LysTrp: 1.221 ± 0.257
2.299LysTyr: 2.299 ± 0.381
0.0LysXaa: 0.0 ± 0.0
Leu
8.55LeuAla: 8.55 ± 0.812
0.503LeuCys: 0.503 ± 0.2
4.958LeuAsp: 4.958 ± 0.49
6.395LeuGlu: 6.395 ± 0.87
2.946LeuPhe: 2.946 ± 0.417
5.748LeuGly: 5.748 ± 0.6
1.509LeuHis: 1.509 ± 0.303
3.449LeuIle: 3.449 ± 0.473
5.461LeuLys: 5.461 ± 0.503
5.676LeuLeu: 5.676 ± 0.878
3.161LeuMet: 3.161 ± 0.503
3.161LeuAsn: 3.161 ± 0.523
4.024LeuPro: 4.024 ± 0.431
3.664LeuGln: 3.664 ± 0.588
4.527LeuArg: 4.527 ± 0.545
5.604LeuSer: 5.604 ± 0.675
4.958LeuThr: 4.958 ± 0.6
6.035LeuVal: 6.035 ± 0.636
1.078LeuTrp: 1.078 ± 0.285
2.515LeuTyr: 2.515 ± 0.385
0.0LeuXaa: 0.0 ± 0.0
Met
2.73MetAla: 2.73 ± 0.337
0.287MetCys: 0.287 ± 0.139
1.581MetAsp: 1.581 ± 0.321
2.084MetGlu: 2.084 ± 0.564
1.293MetPhe: 1.293 ± 0.278
1.653MetGly: 1.653 ± 0.337
0.647MetHis: 0.647 ± 0.203
1.221MetIle: 1.221 ± 0.317
2.443MetLys: 2.443 ± 0.356
2.515MetLeu: 2.515 ± 0.324
1.078MetMet: 1.078 ± 0.249
1.509MetAsn: 1.509 ± 0.256
1.15MetPro: 1.15 ± 0.303
1.796MetGln: 1.796 ± 0.451
1.653MetArg: 1.653 ± 0.343
2.299MetSer: 2.299 ± 0.468
1.796MetThr: 1.796 ± 0.359
1.653MetVal: 1.653 ± 0.327
0.503MetTrp: 0.503 ± 0.187
1.365MetTyr: 1.365 ± 0.248
0.0MetXaa: 0.0 ± 0.0
Asn
3.018AsnAla: 3.018 ± 0.536
0.359AsnCys: 0.359 ± 0.189
1.94AsnAsp: 1.94 ± 0.264
2.299AsnGlu: 2.299 ± 0.459
1.653AsnPhe: 1.653 ± 0.369
3.377AsnGly: 3.377 ± 0.632
0.431AsnHis: 0.431 ± 0.159
2.874AsnIle: 2.874 ± 0.48
2.802AsnLys: 2.802 ± 0.357
3.521AsnLeu: 3.521 ± 0.46
1.509AsnMet: 1.509 ± 0.235
2.443AsnAsn: 2.443 ± 0.574
2.012AsnPro: 2.012 ± 0.439
1.581AsnGln: 1.581 ± 0.265
2.73AsnArg: 2.73 ± 0.357
2.443AsnSer: 2.443 ± 0.363
3.018AsnThr: 3.018 ± 0.578
2.874AsnVal: 2.874 ± 0.58
0.79AsnTrp: 0.79 ± 0.202
1.796AsnTyr: 1.796 ± 0.251
0.0AsnXaa: 0.0 ± 0.0
Pro
3.736ProAla: 3.736 ± 0.424
0.503ProCys: 0.503 ± 0.222
2.73ProAsp: 2.73 ± 0.423
3.736ProGlu: 3.736 ± 0.633
1.221ProPhe: 1.221 ± 0.264
0.359ProGly: 0.359 ± 0.145
0.647ProHis: 0.647 ± 0.185
1.724ProIle: 1.724 ± 0.614
1.724ProLys: 1.724 ± 0.28
2.587ProLeu: 2.587 ± 0.484
0.79ProMet: 0.79 ± 0.231
1.365ProAsn: 1.365 ± 0.322
1.581ProPro: 1.581 ± 0.312
1.94ProGln: 1.94 ± 0.424
0.79ProArg: 0.79 ± 0.237
2.73ProSer: 2.73 ± 0.481
2.658ProThr: 2.658 ± 0.442
3.521ProVal: 3.521 ± 0.496
0.503ProTrp: 0.503 ± 0.202
1.724ProTyr: 1.724 ± 0.447
0.0ProXaa: 0.0 ± 0.0
Gln
5.389GlnAla: 5.389 ± 0.659
0.287GlnCys: 0.287 ± 0.166
2.227GlnAsp: 2.227 ± 0.422
3.664GlnGlu: 3.664 ± 0.388
1.724GlnPhe: 1.724 ± 0.282
2.443GlnGly: 2.443 ± 0.424
0.862GlnHis: 0.862 ± 0.291
1.365GlnIle: 1.365 ± 0.243
2.299GlnLys: 2.299 ± 0.356
5.532GlnLeu: 5.532 ± 0.643
1.796GlnMet: 1.796 ± 0.453
1.581GlnAsn: 1.581 ± 0.525
1.293GlnPro: 1.293 ± 0.298
3.449GlnGln: 3.449 ± 0.714
2.443GlnArg: 2.443 ± 0.384
3.449GlnSer: 3.449 ± 0.656
1.868GlnThr: 1.868 ± 0.327
3.377GlnVal: 3.377 ± 0.595
0.503GlnTrp: 0.503 ± 0.185
1.293GlnTyr: 1.293 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
4.383ArgAla: 4.383 ± 0.684
0.431ArgCys: 0.431 ± 0.173
2.874ArgAsp: 2.874 ± 0.421
2.874ArgGlu: 2.874 ± 0.561
2.012ArgPhe: 2.012 ± 0.316
4.311ArgGly: 4.311 ± 0.458
0.79ArgHis: 0.79 ± 0.246
2.802ArgIle: 2.802 ± 0.395
2.874ArgLys: 2.874 ± 0.385
3.808ArgLeu: 3.808 ± 0.452
1.509ArgMet: 1.509 ± 0.361
1.581ArgAsn: 1.581 ± 0.311
1.94ArgPro: 1.94 ± 0.375
1.724ArgGln: 1.724 ± 0.328
1.653ArgArg: 1.653 ± 0.27
2.515ArgSer: 2.515 ± 0.43
2.155ArgThr: 2.155 ± 0.324
3.09ArgVal: 3.09 ± 0.516
0.647ArgTrp: 0.647 ± 0.239
2.155ArgTyr: 2.155 ± 0.351
0.0ArgXaa: 0.0 ± 0.0
Ser
3.736SerAla: 3.736 ± 0.544
0.934SerCys: 0.934 ± 0.236
3.521SerAsp: 3.521 ± 0.478
4.383SerGlu: 4.383 ± 0.614
2.299SerPhe: 2.299 ± 0.341
5.892SerGly: 5.892 ± 0.685
1.078SerHis: 1.078 ± 0.317
3.377SerIle: 3.377 ± 0.523
3.808SerLys: 3.808 ± 0.539
5.245SerLeu: 5.245 ± 0.749
1.94SerMet: 1.94 ± 0.347
2.587SerAsn: 2.587 ± 0.518
2.658SerPro: 2.658 ± 0.535
2.658SerGln: 2.658 ± 0.384
2.946SerArg: 2.946 ± 0.493
3.592SerSer: 3.592 ± 0.614
3.09SerThr: 3.09 ± 0.456
3.808SerVal: 3.808 ± 0.506
0.718SerTrp: 0.718 ± 0.29
2.443SerTyr: 2.443 ± 0.404
0.0SerXaa: 0.0 ± 0.0
Thr
4.598ThrAla: 4.598 ± 0.471
0.647ThrCys: 0.647 ± 0.244
4.167ThrAsp: 4.167 ± 0.504
3.305ThrGlu: 3.305 ± 0.675
2.155ThrPhe: 2.155 ± 0.335
4.455ThrGly: 4.455 ± 0.556
1.653ThrHis: 1.653 ± 0.369
2.73ThrIle: 2.73 ± 0.316
4.598ThrLys: 4.598 ± 0.571
4.67ThrLeu: 4.67 ± 0.538
1.437ThrMet: 1.437 ± 0.258
1.94ThrAsn: 1.94 ± 0.363
2.658ThrPro: 2.658 ± 0.436
3.161ThrGln: 3.161 ± 0.56
2.155ThrArg: 2.155 ± 0.334
3.161ThrSer: 3.161 ± 0.477
4.167ThrThr: 4.167 ± 0.606
3.808ThrVal: 3.808 ± 0.52
0.431ThrTrp: 0.431 ± 0.219
2.73ThrTyr: 2.73 ± 0.488
0.0ThrXaa: 0.0 ± 0.0
Val
5.676ValAla: 5.676 ± 0.491
1.006ValCys: 1.006 ± 0.29
3.88ValAsp: 3.88 ± 0.561
4.383ValGlu: 4.383 ± 0.541
2.371ValPhe: 2.371 ± 0.437
5.101ValGly: 5.101 ± 0.581
1.15ValHis: 1.15 ± 0.336
3.736ValIle: 3.736 ± 0.398
4.886ValLys: 4.886 ± 0.481
5.317ValLeu: 5.317 ± 0.531
1.868ValMet: 1.868 ± 0.401
3.808ValAsn: 3.808 ± 0.467
2.587ValPro: 2.587 ± 0.446
2.874ValGln: 2.874 ± 0.501
2.874ValArg: 2.874 ± 0.541
4.455ValSer: 4.455 ± 0.5
5.029ValThr: 5.029 ± 0.61
4.814ValVal: 4.814 ± 0.676
0.862ValTrp: 0.862 ± 0.337
2.084ValTyr: 2.084 ± 0.581
0.0ValXaa: 0.0 ± 0.0
Trp
0.934TrpAla: 0.934 ± 0.294
0.431TrpCys: 0.431 ± 0.151
0.862TrpAsp: 0.862 ± 0.321
0.718TrpGlu: 0.718 ± 0.224
0.647TrpPhe: 0.647 ± 0.176
0.79TrpGly: 0.79 ± 0.274
0.431TrpHis: 0.431 ± 0.17
0.503TrpIle: 0.503 ± 0.194
1.078TrpLys: 1.078 ± 0.239
1.509TrpLeu: 1.509 ± 0.285
0.359TrpMet: 0.359 ± 0.206
0.934TrpAsn: 0.934 ± 0.206
0.503TrpPro: 0.503 ± 0.224
0.934TrpGln: 0.934 ± 0.228
0.647TrpArg: 0.647 ± 0.21
1.006TrpSer: 1.006 ± 0.238
0.647TrpThr: 0.647 ± 0.223
0.647TrpVal: 0.647 ± 0.202
0.072TrpTrp: 0.072 ± 0.069
0.287TrpTyr: 0.287 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.233TyrAla: 3.233 ± 0.363
0.359TyrCys: 0.359 ± 0.149
2.299TyrAsp: 2.299 ± 0.403
1.94TyrGlu: 1.94 ± 0.306
2.012TyrPhe: 2.012 ± 0.281
3.09TyrGly: 3.09 ± 0.404
0.79TyrHis: 0.79 ± 0.262
2.443TyrIle: 2.443 ± 0.488
2.155TyrLys: 2.155 ± 0.496
2.658TyrLeu: 2.658 ± 0.38
0.934TyrMet: 0.934 ± 0.282
1.796TyrAsn: 1.796 ± 0.311
1.796TyrPro: 1.796 ± 0.471
1.653TyrGln: 1.653 ± 0.326
1.94TyrArg: 1.94 ± 0.37
2.012TyrSer: 2.012 ± 0.424
2.012TyrThr: 2.012 ± 0.336
3.018TyrVal: 3.018 ± 0.526
0.431TyrTrp: 0.431 ± 0.173
1.006TyrTyr: 1.006 ± 0.257
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (13919 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski