Rhodovulum sp. P5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodovulum; unclassified Rhodovulum

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0PVT6|A0A1V0PVT6_9RHOB Uncharacterized protein OS=Rhodovulum sp. P5 OX=1564506 GN=RGUI_2111 PE=4 SV=1
MM1 pKa = 7.48IAGLALGAGMAHH13 pKa = 7.29AASVSPVSYY22 pKa = 11.52DD23 pKa = 3.4MLDD26 pKa = 3.54GGTGSYY32 pKa = 10.26QYY34 pKa = 10.91WDD36 pKa = 3.34DD37 pKa = 4.12TYY39 pKa = 11.07PGNNNTVSYY48 pKa = 11.08DD49 pKa = 3.44SLTGGTGDD57 pKa = 3.8LTDD60 pKa = 4.7GVIATDD66 pKa = 2.63NWFITEE72 pKa = 4.33APAGPGPYY80 pKa = 9.95VGWSNYY86 pKa = 9.73NPVITFNFATAYY98 pKa = 10.25DD99 pKa = 3.88FLSATFHH106 pKa = 7.34FDD108 pKa = 4.17DD109 pKa = 4.44SDD111 pKa = 4.34GNGGVSQPRR120 pKa = 11.84SVNINGTDD128 pKa = 3.49YY129 pKa = 10.74TVPTNDD135 pKa = 3.02GSAPFAFTADD145 pKa = 3.86LAGLTTDD152 pKa = 3.87TLVVTITRR160 pKa = 11.84SNSWVFLSEE169 pKa = 4.06VEE171 pKa = 3.76FDD173 pKa = 4.19ARR175 pKa = 11.84IDD177 pKa = 3.74NQVPLPASVWLLAAGLGGLGLTRR200 pKa = 11.84ARR202 pKa = 11.84RR203 pKa = 11.84KK204 pKa = 8.73VV205 pKa = 3.07

Molecular weight: 21.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0PYF8|A0A1V0PYF8_9RHOB Uncharacterized protein ImpI/VasC OS=Rhodovulum sp. P5 OX=1564506 GN=RGUI_3042 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.43GGRR28 pKa = 11.84KK29 pKa = 8.99VLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4261

0

4261

1307351

37

7038

306.8

33.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.847 ± 0.06

0.895 ± 0.015

6.351 ± 0.072

5.907 ± 0.039

3.652 ± 0.025

8.931 ± 0.058

2.03 ± 0.02

4.913 ± 0.029

2.885 ± 0.036

10.051 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.66 ± 0.025

2.359 ± 0.024

5.274 ± 0.038

2.913 ± 0.026

7.029 ± 0.058

4.843 ± 0.03

5.599 ± 0.059

7.357 ± 0.033

1.372 ± 0.018

2.133 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski