Amino acid dipepetide frequency for Rhodovulum sp. P5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.029AlaAla: 18.029 ± 0.194
1.205AlaCys: 1.205 ± 0.034
7.801AlaAsp: 7.801 ± 0.123
9.076AlaGlu: 9.076 ± 0.11
4.448AlaPhe: 4.448 ± 0.056
11.391AlaGly: 11.391 ± 0.179
2.482AlaHis: 2.482 ± 0.048
5.799AlaIle: 5.799 ± 0.066
3.505AlaLys: 3.505 ± 0.071
14.386AlaLeu: 14.386 ± 0.149
3.79AlaMet: 3.79 ± 0.059
2.581AlaAsn: 2.581 ± 0.042
6.261AlaPro: 6.261 ± 0.086
3.974AlaGln: 3.974 ± 0.07
9.558AlaArg: 9.558 ± 0.114
5.247AlaSer: 5.247 ± 0.067
6.201AlaThr: 6.201 ± 0.084
8.739AlaVal: 8.739 ± 0.111
1.507AlaTrp: 1.507 ± 0.035
2.491AlaTyr: 2.491 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
1.088CysAla: 1.088 ± 0.034
0.113CysCys: 0.113 ± 0.01
0.627CysAsp: 0.627 ± 0.022
0.462CysGlu: 0.462 ± 0.021
0.296CysPhe: 0.296 ± 0.016
0.947CysGly: 0.947 ± 0.03
0.293CysHis: 0.293 ± 0.017
0.405CysIle: 0.405 ± 0.017
0.202CysLys: 0.202 ± 0.012
0.877CysLeu: 0.877 ± 0.026
0.168CysMet: 0.168 ± 0.011
0.212CysAsn: 0.212 ± 0.012
0.565CysPro: 0.565 ± 0.021
0.22CysGln: 0.22 ± 0.012
0.637CysArg: 0.637 ± 0.024
0.438CysSer: 0.438 ± 0.018
0.466CysThr: 0.466 ± 0.022
0.617CysVal: 0.617 ± 0.02
0.112CysTrp: 0.112 ± 0.01
0.203CysTyr: 0.203 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
7.547AspAla: 7.547 ± 0.133
0.552AspCys: 0.552 ± 0.021
3.865AspAsp: 3.865 ± 0.171
3.46AspGlu: 3.46 ± 0.051
2.415AspPhe: 2.415 ± 0.045
6.136AspGly: 6.136 ± 0.219
1.407AspHis: 1.407 ± 0.038
3.38AspIle: 3.38 ± 0.074
1.57AspLys: 1.57 ± 0.038
6.831AspLeu: 6.831 ± 0.079
1.727AspMet: 1.727 ± 0.038
1.305AspAsn: 1.305 ± 0.041
3.954AspPro: 3.954 ± 0.066
1.648AspGln: 1.648 ± 0.034
4.9AspArg: 4.9 ± 0.068
2.383AspSer: 2.383 ± 0.106
3.389AspThr: 3.389 ± 0.175
4.247AspVal: 4.247 ± 0.059
1.248AspTrp: 1.248 ± 0.038
1.544AspTyr: 1.544 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
8.383GluAla: 8.383 ± 0.108
0.366GluCys: 0.366 ± 0.015
3.699GluAsp: 3.699 ± 0.057
3.722GluGlu: 3.722 ± 0.082
1.792GluPhe: 1.792 ± 0.038
4.899GluGly: 4.899 ± 0.065
1.038GluHis: 1.038 ± 0.032
3.765GluIle: 3.765 ± 0.063
2.108GluLys: 2.108 ± 0.052
4.835GluLeu: 4.835 ± 0.073
1.825GluMet: 1.825 ± 0.04
1.603GluAsn: 1.603 ± 0.038
2.574GluPro: 2.574 ± 0.054
1.744GluGln: 1.744 ± 0.042
4.478GluArg: 4.478 ± 0.08
2.171GluSer: 2.171 ± 0.043
4.166GluThr: 4.166 ± 0.067
4.326GluVal: 4.326 ± 0.059
0.631GluTrp: 0.631 ± 0.024
0.939GluTyr: 0.939 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
4.564PheAla: 4.564 ± 0.067
0.401PheCys: 0.401 ± 0.018
2.89PheAsp: 2.89 ± 0.042
2.197PheGlu: 2.197 ± 0.048
1.417PhePhe: 1.417 ± 0.035
3.603PheGly: 3.603 ± 0.063
0.737PheHis: 0.737 ± 0.024
1.456PheIle: 1.456 ± 0.04
0.811PheLys: 0.811 ± 0.027
3.426PheLeu: 3.426 ± 0.052
0.804PheMet: 0.804 ± 0.031
0.967PheAsn: 0.967 ± 0.026
1.606PhePro: 1.606 ± 0.039
1.018PheGln: 1.018 ± 0.032
2.335PheArg: 2.335 ± 0.046
2.055PheSer: 2.055 ± 0.047
2.162PheThr: 2.162 ± 0.065
2.668PheVal: 2.668 ± 0.051
0.537PheTrp: 0.537 ± 0.022
0.862PheTyr: 0.862 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
10.539GlyAla: 10.539 ± 0.14
0.916GlyCys: 0.916 ± 0.034
5.27GlyAsp: 5.27 ± 0.264
4.809GlyGlu: 4.809 ± 0.062
3.577GlyPhe: 3.577 ± 0.062
7.717GlyGly: 7.717 ± 0.204
2.058GlyHis: 2.058 ± 0.042
4.335GlyIle: 4.335 ± 0.064
2.961GlyLys: 2.961 ± 0.055
9.521GlyLeu: 9.521 ± 0.129
2.531GlyMet: 2.531 ± 0.053
2.036GlyAsn: 2.036 ± 0.071
4.073GlyPro: 4.073 ± 0.066
3.227GlyGln: 3.227 ± 0.056
6.323GlyArg: 6.323 ± 0.089
3.997GlySer: 3.997 ± 0.081
5.23GlyThr: 5.23 ± 0.144
6.435GlyVal: 6.435 ± 0.074
1.537GlyTrp: 1.537 ± 0.038
2.221GlyTyr: 2.221 ± 0.043
0.0GlyXaa: 0.0 ± 0.0
His
2.557HisAla: 2.557 ± 0.06
0.217HisCys: 0.217 ± 0.013
1.348HisAsp: 1.348 ± 0.032
1.02HisGlu: 1.02 ± 0.028
0.809HisPhe: 0.809 ± 0.027
1.95HisGly: 1.95 ± 0.048
0.494HisHis: 0.494 ± 0.02
0.898HisIle: 0.898 ± 0.028
0.482HisLys: 0.482 ± 0.019
2.05HisLeu: 2.05 ± 0.047
0.537HisMet: 0.537 ± 0.021
0.392HisAsn: 0.392 ± 0.016
1.461HisPro: 1.461 ± 0.041
0.51HisGln: 0.51 ± 0.018
1.455HisArg: 1.455 ± 0.039
0.881HisSer: 0.881 ± 0.027
0.816HisThr: 0.816 ± 0.025
1.589HisVal: 1.589 ± 0.038
0.347HisTrp: 0.347 ± 0.02
0.486HisTyr: 0.486 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
6.981IleAla: 6.981 ± 0.067
0.553IleCys: 0.553 ± 0.021
3.659IleAsp: 3.659 ± 0.067
3.417IleGlu: 3.417 ± 0.048
1.64IlePhe: 1.64 ± 0.038
4.77IleGly: 4.77 ± 0.067
0.934IleHis: 0.934 ± 0.025
1.775IleIle: 1.775 ± 0.039
1.201IleLys: 1.201 ± 0.032
4.643IleLeu: 4.643 ± 0.069
0.939IleMet: 0.939 ± 0.032
1.144IleAsn: 1.144 ± 0.037
2.253IlePro: 2.253 ± 0.047
1.055IleGln: 1.055 ± 0.028
3.16IleArg: 3.16 ± 0.057
2.676IleSer: 2.676 ± 0.049
2.803IleThr: 2.803 ± 0.092
3.828IleVal: 3.828 ± 0.063
0.68IleTrp: 0.68 ± 0.024
1.016IleTyr: 1.016 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.84LysAla: 3.84 ± 0.069
0.174LysCys: 0.174 ± 0.013
1.622LysAsp: 1.622 ± 0.044
1.422LysGlu: 1.422 ± 0.039
0.769LysPhe: 0.769 ± 0.026
2.604LysGly: 2.604 ± 0.055
0.525LysHis: 0.525 ± 0.021
1.502LysIle: 1.502 ± 0.036
1.046LysLys: 1.046 ± 0.038
2.723LysLeu: 2.723 ± 0.052
0.778LysMet: 0.778 ± 0.027
0.672LysAsn: 0.672 ± 0.024
1.715LysPro: 1.715 ± 0.045
0.77LysGln: 0.77 ± 0.027
2.113LysArg: 2.113 ± 0.046
1.56LysSer: 1.56 ± 0.037
1.947LysThr: 1.947 ± 0.045
2.132LysVal: 2.132 ± 0.052
0.354LysTrp: 0.354 ± 0.018
0.584LysTyr: 0.584 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
13.54LeuAla: 13.54 ± 0.15
0.965LeuCys: 0.965 ± 0.031
6.216LeuAsp: 6.216 ± 0.091
5.188LeuGlu: 5.188 ± 0.076
3.551LeuPhe: 3.551 ± 0.061
8.638LeuGly: 8.638 ± 0.116
1.838LeuHis: 1.838 ± 0.042
5.101LeuIle: 5.101 ± 0.069
3.151LeuLys: 3.151 ± 0.058
8.773LeuLeu: 8.773 ± 0.114
2.491LeuMet: 2.491 ± 0.053
2.522LeuAsn: 2.522 ± 0.051
5.701LeuPro: 5.701 ± 0.086
2.585LeuGln: 2.585 ± 0.05
7.078LeuArg: 7.078 ± 0.09
6.739LeuSer: 6.739 ± 0.084
6.203LeuThr: 6.203 ± 0.085
6.981LeuVal: 6.981 ± 0.087
1.301LeuTrp: 1.301 ± 0.035
1.944LeuTyr: 1.944 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
3.448MetAla: 3.448 ± 0.067
0.187MetCys: 0.187 ± 0.011
1.352MetAsp: 1.352 ± 0.032
1.414MetGlu: 1.414 ± 0.037
0.758MetPhe: 0.758 ± 0.026
2.285MetGly: 2.285 ± 0.049
0.499MetHis: 0.499 ± 0.021
1.433MetIle: 1.433 ± 0.039
0.987MetLys: 0.987 ± 0.027
2.51MetLeu: 2.51 ± 0.055
0.702MetMet: 0.702 ± 0.025
0.745MetAsn: 0.745 ± 0.025
1.508MetPro: 1.508 ± 0.036
0.912MetGln: 0.912 ± 0.03
1.888MetArg: 1.888 ± 0.039
1.576MetSer: 1.576 ± 0.031
2.06MetThr: 2.06 ± 0.041
1.784MetVal: 1.784 ± 0.048
0.228MetTrp: 0.228 ± 0.013
0.32MetTyr: 0.32 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.05AsnAla: 3.05 ± 0.049
0.249AsnCys: 0.249 ± 0.015
1.437AsnAsp: 1.437 ± 0.059
1.049AsnGlu: 1.049 ± 0.028
0.862AsnPhe: 0.862 ± 0.028
2.269AsnGly: 2.269 ± 0.06
0.467AsnHis: 0.467 ± 0.024
1.171AsnIle: 1.171 ± 0.032
0.532AsnLys: 0.532 ± 0.019
2.367AsnLeu: 2.367 ± 0.042
0.63AsnMet: 0.63 ± 0.022
0.586AsnAsn: 0.586 ± 0.024
1.695AsnPro: 1.695 ± 0.041
0.574AsnGln: 0.574 ± 0.023
1.77AsnArg: 1.77 ± 0.04
0.945AsnSer: 0.945 ± 0.033
1.24AsnThr: 1.24 ± 0.043
1.752AsnVal: 1.752 ± 0.043
0.382AsnTrp: 0.382 ± 0.018
0.566AsnTyr: 0.566 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
6.398ProAla: 6.398 ± 0.094
0.412ProCys: 0.412 ± 0.019
4.449ProAsp: 4.449 ± 0.069
4.13ProGlu: 4.13 ± 0.064
1.986ProPhe: 1.986 ± 0.048
4.87ProGly: 4.87 ± 0.076
1.034ProHis: 1.034 ± 0.031
2.075ProIle: 2.075 ± 0.039
1.599ProLys: 1.599 ± 0.04
4.811ProLeu: 4.811 ± 0.07
1.264ProMet: 1.264 ± 0.034
1.222ProAsn: 1.222 ± 0.027
2.702ProPro: 2.702 ± 0.07
1.534ProGln: 1.534 ± 0.032
3.075ProArg: 3.075 ± 0.062
2.365ProSer: 2.365 ± 0.04
2.434ProThr: 2.434 ± 0.05
4.537ProVal: 4.537 ± 0.067
0.678ProTrp: 0.678 ± 0.025
1.163ProTyr: 1.163 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
3.935GlnAla: 3.935 ± 0.072
0.201GlnCys: 0.201 ± 0.013
1.627GlnAsp: 1.627 ± 0.043
1.497GlnGlu: 1.497 ± 0.033
1.034GlnPhe: 1.034 ± 0.032
2.487GlnGly: 2.487 ± 0.048
0.519GlnHis: 0.519 ± 0.02
1.76GlnIle: 1.76 ± 0.037
0.917GlnLys: 0.917 ± 0.03
2.398GlnLeu: 2.398 ± 0.048
0.95GlnMet: 0.95 ± 0.029
0.801GlnAsn: 0.801 ± 0.027
1.53GlnPro: 1.53 ± 0.033
0.928GlnGln: 0.928 ± 0.038
2.103GlnArg: 2.103 ± 0.049
1.543GlnSer: 1.543 ± 0.034
1.749GlnThr: 1.749 ± 0.042
2.287GlnVal: 2.287 ± 0.051
0.318GlnTrp: 0.318 ± 0.016
0.548GlnTyr: 0.548 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
8.646ArgAla: 8.646 ± 0.106
0.518ArgCys: 0.518 ± 0.022
4.364ArgAsp: 4.364 ± 0.072
3.849ArgGlu: 3.849 ± 0.072
2.881ArgPhe: 2.881 ± 0.047
4.811ArgGly: 4.811 ± 0.067
1.662ArgHis: 1.662 ± 0.046
3.915ArgIle: 3.915 ± 0.062
2.301ArgLys: 2.301 ± 0.047
7.957ArgLeu: 7.957 ± 0.099
2.061ArgMet: 2.061 ± 0.041
1.674ArgAsn: 1.674 ± 0.04
3.789ArgPro: 3.789 ± 0.062
2.431ArgGln: 2.431 ± 0.049
5.603ArgArg: 5.603 ± 0.086
3.126ArgSer: 3.126 ± 0.06
3.175ArgThr: 3.175 ± 0.065
4.973ArgVal: 4.973 ± 0.071
1.001ArgTrp: 1.001 ± 0.03
1.557ArgTyr: 1.557 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
5.735SerAla: 5.735 ± 0.08
0.418SerCys: 0.418 ± 0.02
3.155SerAsp: 3.155 ± 0.063
2.671SerGlu: 2.671 ± 0.056
2.107SerPhe: 2.107 ± 0.051
5.422SerGly: 5.422 ± 0.096
1.047SerHis: 1.047 ± 0.03
2.333SerIle: 2.333 ± 0.049
1.263SerLys: 1.263 ± 0.036
4.723SerLeu: 4.723 ± 0.072
1.259SerMet: 1.259 ± 0.034
1.184SerAsn: 1.184 ± 0.033
2.455SerPro: 2.455 ± 0.046
1.289SerGln: 1.289 ± 0.035
3.067SerArg: 3.067 ± 0.049
2.391SerSer: 2.391 ± 0.059
2.32SerThr: 2.32 ± 0.049
3.801SerVal: 3.801 ± 0.073
0.627SerTrp: 0.627 ± 0.035
1.163SerTyr: 1.163 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
6.808ThrAla: 6.808 ± 0.088
0.492ThrCys: 0.492 ± 0.021
3.562ThrAsp: 3.562 ± 0.151
3.042ThrGlu: 3.042 ± 0.05
1.921ThrPhe: 1.921 ± 0.044
5.942ThrGly: 5.942 ± 0.115
1.054ThrHis: 1.054 ± 0.028
2.668ThrIle: 2.668 ± 0.086
1.291ThrLys: 1.291 ± 0.034
6.288ThrLeu: 6.288 ± 0.13
1.303ThrMet: 1.303 ± 0.029
1.187ThrAsn: 1.187 ± 0.042
3.391ThrPro: 3.391 ± 0.055
1.464ThrGln: 1.464 ± 0.044
3.592ThrArg: 3.592 ± 0.065
2.415ThrSer: 2.415 ± 0.056
2.813ThrThr: 2.813 ± 0.065
4.709ThrVal: 4.709 ± 0.144
0.699ThrTrp: 0.699 ± 0.026
1.352ThrTyr: 1.352 ± 0.056
0.0ThrXaa: 0.0 ± 0.0
Val
9.466ValAla: 9.466 ± 0.1
0.655ValCys: 0.655 ± 0.026
4.163ValAsp: 4.163 ± 0.072
4.788ValGlu: 4.788 ± 0.068
2.885ValPhe: 2.885 ± 0.047
5.445ValGly: 5.445 ± 0.073
1.378ValHis: 1.378 ± 0.038
3.927ValIle: 3.927 ± 0.061
1.966ValLys: 1.966 ± 0.045
7.662ValLeu: 7.662 ± 0.108
1.99ValMet: 1.99 ± 0.04
1.847ValAsn: 1.847 ± 0.053
3.758ValPro: 3.758 ± 0.066
2.082ValGln: 2.082 ± 0.041
4.397ValArg: 4.397 ± 0.071
4.049ValSer: 4.049 ± 0.057
4.928ValThr: 4.928 ± 0.115
5.708ValVal: 5.708 ± 0.079
0.99ValTrp: 0.99 ± 0.03
1.486ValTyr: 1.486 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.475TrpAla: 1.475 ± 0.035
0.131TrpCys: 0.131 ± 0.01
0.784TrpAsp: 0.784 ± 0.032
0.633TrpGlu: 0.633 ± 0.022
0.503TrpPhe: 0.503 ± 0.02
1.027TrpGly: 1.027 ± 0.03
0.33TrpHis: 0.33 ± 0.016
0.605TrpIle: 0.605 ± 0.023
0.425TrpLys: 0.425 ± 0.016
1.593TrpLeu: 1.593 ± 0.04
0.391TrpMet: 0.391 ± 0.018
0.354TrpAsn: 0.354 ± 0.016
0.729TrpPro: 0.729 ± 0.028
0.574TrpGln: 0.574 ± 0.022
1.169TrpArg: 1.169 ± 0.033
0.782TrpSer: 0.782 ± 0.029
0.769TrpThr: 0.769 ± 0.025
0.953TrpVal: 0.953 ± 0.027
0.201TrpTrp: 0.201 ± 0.013
0.29TrpTyr: 0.29 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.442TyrAla: 2.442 ± 0.046
0.222TyrCys: 0.222 ± 0.013
1.615TyrAsp: 1.615 ± 0.041
1.22TyrGlu: 1.22 ± 0.042
0.864TyrPhe: 0.864 ± 0.026
2.101TyrGly: 2.101 ± 0.041
0.501TyrHis: 0.501 ± 0.021
0.823TyrIle: 0.823 ± 0.026
0.533TyrLys: 0.533 ± 0.022
2.13TyrLeu: 2.13 ± 0.042
0.457TyrMet: 0.457 ± 0.02
0.557TyrAsn: 0.557 ± 0.023
1.021TyrPro: 1.021 ± 0.031
0.594TyrGln: 0.594 ± 0.024
1.593TyrArg: 1.593 ± 0.042
1.092TyrSer: 1.092 ± 0.033
1.123TyrThr: 1.123 ± 0.049
1.508TyrVal: 1.508 ± 0.036
0.338TyrTrp: 0.338 ± 0.016
0.589TyrTyr: 0.589 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4261 proteins (1307351 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski