Streptococcus phage Javan527

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D6AGV5|A0A4D6AGV5_9CAUD Uncharacterized protein OS=Streptococcus phage Javan527 OX=2548240 GN=Javan527_0071 PE=4 SV=1
MM1 pKa = 7.55IPNFRR6 pKa = 11.84AFNKK10 pKa = 9.9KK11 pKa = 7.2IQKK14 pKa = 9.28MYY16 pKa = 10.77GVDD19 pKa = 3.38GFKK22 pKa = 10.66SSEE25 pKa = 3.76RR26 pKa = 11.84KK27 pKa = 9.31IYY29 pKa = 10.26RR30 pKa = 11.84CSLADD35 pKa = 3.43DD36 pKa = 4.18EE37 pKa = 4.8FRR39 pKa = 11.84SGRR42 pKa = 11.84LEE44 pKa = 3.85TFHH47 pKa = 7.61FVEE50 pKa = 5.77DD51 pKa = 3.96NLDD54 pKa = 4.08DD55 pKa = 4.74YY56 pKa = 11.72ILMQSTGLFDD66 pKa = 4.23KK67 pKa = 11.22NGVEE71 pKa = 3.93IFEE74 pKa = 4.64GDD76 pKa = 3.57VVEE79 pKa = 4.7YY80 pKa = 10.98DD81 pKa = 3.24DD82 pKa = 6.11GEE84 pKa = 4.3YY85 pKa = 10.96LFAGKK90 pKa = 8.98VVKK93 pKa = 9.3TVFGTYY99 pKa = 9.05VKK101 pKa = 10.17SYY103 pKa = 10.87SFFSFEE109 pKa = 5.09DD110 pKa = 4.3FSDD113 pKa = 4.13EE114 pKa = 4.07NTMTADD120 pKa = 3.34VEE122 pKa = 4.63IIGNIYY128 pKa = 9.78EE129 pKa = 4.24EE130 pKa = 4.59SVEE133 pKa = 4.19EE134 pKa = 4.1

Molecular weight:
15.56 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D6AHN7|A0A4D6AHN7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan527 OX=2548240 GN=Javan527_0015 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 10.03RR4 pKa = 11.84IKK6 pKa = 10.22KK7 pKa = 9.09KK8 pKa = 10.34RR9 pKa = 11.84KK10 pKa = 9.22LEE12 pKa = 4.06TAVVMLVAEE21 pKa = 4.59NAMQAKK27 pKa = 10.09ALRR30 pKa = 11.84NQNKK34 pKa = 9.65QIAEE38 pKa = 4.03LRR40 pKa = 11.84AIIQQNVQAINRR52 pKa = 11.84EE53 pKa = 3.91FVTAKK58 pKa = 9.31NTILDD63 pKa = 3.57NQLAIKK69 pKa = 10.46SIGDD73 pKa = 3.61DD74 pKa = 3.59VNHH77 pKa = 7.08IKK79 pKa = 10.52QNYY82 pKa = 6.74KK83 pKa = 10.47RR84 pKa = 11.84KK85 pKa = 8.22WRR87 pKa = 11.84KK88 pKa = 8.88

Molecular weight:
10.28 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

13466

40

1211

179.5

20.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.04 ± 0.838

0.505 ± 0.091

6.379 ± 0.354

7.634 ± 0.596

3.876 ± 0.189

6.015 ± 0.499

1.478 ± 0.146

7.003 ± 0.225

9.171 ± 0.437

8.57 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.755 ± 0.166

5.213 ± 0.275

2.436 ± 0.18

4.367 ± 0.228

4.166 ± 0.247

6.312 ± 0.502

6.327 ± 0.223

6.208 ± 0.22

1.017 ± 0.116

3.527 ± 0.315

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski