Streptococcus phage Javan527
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AGV5|A0A4D6AGV5_9CAUD Uncharacterized protein OS=Streptococcus phage Javan527 OX=2548240 GN=Javan527_0071 PE=4 SV=1
MM1 pKa = 7.55 IPNFRR6 pKa = 11.84 AFNKK10 pKa = 9.9 KK11 pKa = 7.2 IQKK14 pKa = 9.28 MYY16 pKa = 10.77 GVDD19 pKa = 3.38 GFKK22 pKa = 10.66 SSEE25 pKa = 3.76 RR26 pKa = 11.84 KK27 pKa = 9.31 IYY29 pKa = 10.26 RR30 pKa = 11.84 CSLADD35 pKa = 3.43 DD36 pKa = 4.18 EE37 pKa = 4.8 FRR39 pKa = 11.84 SGRR42 pKa = 11.84 LEE44 pKa = 3.85 TFHH47 pKa = 7.61 FVEE50 pKa = 5.77 DD51 pKa = 3.96 NLDD54 pKa = 4.08 DD55 pKa = 4.74 YY56 pKa = 11.72 ILMQSTGLFDD66 pKa = 4.23 KK67 pKa = 11.22 NGVEE71 pKa = 3.93 IFEE74 pKa = 4.64 GDD76 pKa = 3.57 VVEE79 pKa = 4.7 YY80 pKa = 10.98 DD81 pKa = 3.24 DD82 pKa = 6.11 GEE84 pKa = 4.3 YY85 pKa = 10.96 LFAGKK90 pKa = 8.98 VVKK93 pKa = 9.3 TVFGTYY99 pKa = 9.05 VKK101 pKa = 10.17 SYY103 pKa = 10.87 SFFSFEE109 pKa = 5.09 DD110 pKa = 4.3 FSDD113 pKa = 4.13 EE114 pKa = 4.07 NTMTADD120 pKa = 3.34 VEE122 pKa = 4.63 IIGNIYY128 pKa = 9.78 EE129 pKa = 4.24 EE130 pKa = 4.59 SVEE133 pKa = 4.19 EE134 pKa = 4.1
Molecular weight: 15.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.349
IPC2_protein 4.304
IPC_protein 4.228
Toseland 4.05
ProMoST 4.317
Dawson 4.19
Bjellqvist 4.38
Wikipedia 4.075
Rodwell 4.062
Grimsley 3.961
Solomon 4.177
Lehninger 4.139
Nozaki 4.291
DTASelect 4.469
Thurlkill 4.075
EMBOSS 4.088
Sillero 4.342
Patrickios 3.617
IPC_peptide 4.19
IPC2_peptide 4.329
IPC2.peptide.svr19 4.265
Protein with the highest isoelectric point:
>tr|A0A4D6AHN7|A0A4D6AHN7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan527 OX=2548240 GN=Javan527_0015 PE=4 SV=1
MM1 pKa = 7.55 NKK3 pKa = 10.03 RR4 pKa = 11.84 IKK6 pKa = 10.22 KK7 pKa = 9.09 KK8 pKa = 10.34 RR9 pKa = 11.84 KK10 pKa = 9.22 LEE12 pKa = 4.06 TAVVMLVAEE21 pKa = 4.59 NAMQAKK27 pKa = 10.09 ALRR30 pKa = 11.84 NQNKK34 pKa = 9.65 QIAEE38 pKa = 4.03 LRR40 pKa = 11.84 AIIQQNVQAINRR52 pKa = 11.84 EE53 pKa = 3.91 FVTAKK58 pKa = 9.31 NTILDD63 pKa = 3.57 NQLAIKK69 pKa = 10.46 SIGDD73 pKa = 3.61 DD74 pKa = 3.59 VNHH77 pKa = 7.08 IKK79 pKa = 10.52 QNYY82 pKa = 6.74 KK83 pKa = 10.47 RR84 pKa = 11.84 KK85 pKa = 8.22 WRR87 pKa = 11.84 KK88 pKa = 8.88
Molecular weight: 10.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.208
IPC2_protein 9.794
IPC_protein 10.058
Toseland 11.038
ProMoST 10.613
Dawson 11.082
Bjellqvist 10.701
Wikipedia 11.228
Rodwell 11.594
Grimsley 11.111
Solomon 11.184
Lehninger 11.169
Nozaki 11.008
DTASelect 10.701
Thurlkill 11.008
EMBOSS 11.418
Sillero 11.008
Patrickios 11.33
IPC_peptide 11.199
IPC2_peptide 9.077
IPC2.peptide.svr19 8.776
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
75
0
75
13466
40
1211
179.5
20.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.04 ± 0.838
0.505 ± 0.091
6.379 ± 0.354
7.634 ± 0.596
3.876 ± 0.189
6.015 ± 0.499
1.478 ± 0.146
7.003 ± 0.225
9.171 ± 0.437
8.57 ± 0.282
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.755 ± 0.166
5.213 ± 0.275
2.436 ± 0.18
4.367 ± 0.228
4.166 ± 0.247
6.312 ± 0.502
6.327 ± 0.223
6.208 ± 0.22
1.017 ± 0.116
3.527 ± 0.315
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here