Sphingobacterium olei

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4341 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U0NKD8|A0A4U0NKD8_9SPHI Uncharacterized protein OS=Sphingobacterium olei OX=2571155 GN=FAZ15_15115 PE=4 SV=1
AA1 pKa = 7.03GAVYY5 pKa = 10.26NVAVAKK11 pKa = 10.87GKK13 pKa = 10.1DD14 pKa = 3.32PKK16 pKa = 11.17DD17 pKa = 3.78RR18 pKa = 11.84DD19 pKa = 3.87VEE21 pKa = 4.51GSSTDD26 pKa = 3.66NNPLDD31 pKa = 4.25PTDD34 pKa = 4.47PDD36 pKa = 4.03NPPVDD41 pKa = 4.38PNCPEE46 pKa = 4.03CTIVEE51 pKa = 4.16IDD53 pKa = 3.38QEE55 pKa = 4.45GAIEE59 pKa = 4.32VIKK62 pKa = 9.57TADD65 pKa = 2.9LSQRR69 pKa = 11.84FRR71 pKa = 11.84YY72 pKa = 10.04AGEE75 pKa = 3.85QIAYY79 pKa = 8.48EE80 pKa = 3.96ILIKK84 pKa = 10.95NVGNVTLHH92 pKa = 6.94DD93 pKa = 4.0IKK95 pKa = 11.07VTDD98 pKa = 4.36ANADD102 pKa = 3.66DD103 pKa = 4.41VNVGNIANLAVGEE116 pKa = 4.46SVTLQAYY123 pKa = 7.37HH124 pKa = 7.49TITQADD130 pKa = 3.86MEE132 pKa = 4.55RR133 pKa = 11.84GYY135 pKa = 11.27VSNVAIASGKK145 pKa = 9.83DD146 pKa = 3.08PKK148 pKa = 11.13DD149 pKa = 3.48RR150 pKa = 11.84DD151 pKa = 3.98VIDD154 pKa = 4.22EE155 pKa = 4.36SVSGNVPEE163 pKa = 5.49AGDD166 pKa = 5.12PIDD169 pKa = 5.08PDD171 pKa = 3.84CTNCTIAPLPWKK183 pKa = 10.42VIEE186 pKa = 4.87ANNDD190 pKa = 2.67TPAPVNGKK198 pKa = 9.81DD199 pKa = 3.42GSTTPSVLDD208 pKa = 3.34NDD210 pKa = 4.49MLNGSPVIPAEE221 pKa = 4.22VTLTPGTSPHH231 pKa = 6.29TGIVMNPDD239 pKa = 2.82GTVTVSAEE247 pKa = 4.39TPAGTYY253 pKa = 7.51TYY255 pKa = 9.55PYY257 pKa = 8.94TICEE261 pKa = 4.03VLNPSNCDD269 pKa = 3.28NAVVTILVEE278 pKa = 3.93AAVIEE283 pKa = 4.5ANNDD287 pKa = 2.73TPAPVNGKK295 pKa = 9.95DD296 pKa = 3.28GGTTPSVLDD305 pKa = 3.4NDD307 pKa = 4.49MLNGSPVIPAEE318 pKa = 4.22VTLTPGTSPHH328 pKa = 6.29TGIVMNPDD336 pKa = 2.82GTVTVSAEE344 pKa = 4.39TPAGTYY350 pKa = 7.51TYY352 pKa = 9.55PYY354 pKa = 8.94TICEE358 pKa = 4.03VLNPSNCDD366 pKa = 3.28NAVVTILVEE375 pKa = 3.93AAVIEE380 pKa = 4.5ANNDD384 pKa = 2.73TPAPVNGKK392 pKa = 9.95DD393 pKa = 3.28GGTTPSVLDD402 pKa = 3.4NDD404 pKa = 4.49MLNGSPVIPSEE415 pKa = 4.21VTLTPGTSPHH425 pKa = 6.36TGITMNPDD433 pKa = 2.74GTVTVSAEE441 pKa = 4.39TPAGTYY447 pKa = 7.51TYY449 pKa = 9.55PYY451 pKa = 8.94TICEE455 pKa = 4.03VLNPSNCDD463 pKa = 3.28NAVMTIVVEE472 pKa = 4.09AAVIEE477 pKa = 4.7ANDD480 pKa = 3.96DD481 pKa = 3.42TPAPINGKK489 pKa = 10.01DD490 pKa = 3.34GGTTPSVLDD499 pKa = 3.4NDD501 pKa = 4.49MLNGSPVIPSEE512 pKa = 4.31VTLTWSPDD520 pKa = 3.42NVEE523 pKa = 4.29SPAIILNTDD532 pKa = 3.17GTVTVLPEE540 pKa = 4.18TPEE543 pKa = 4.48GIYY546 pKa = 10.16EE547 pKa = 3.87FSYY550 pKa = 10.51RR551 pKa = 11.84ICEE554 pKa = 4.03VLNPTNCDD562 pKa = 2.9EE563 pKa = 4.22AAITIVIEE571 pKa = 3.93PAPIVAMDD579 pKa = 4.66DD580 pKa = 3.79NIQIEE585 pKa = 4.26WDD587 pKa = 3.43RR588 pKa = 11.84TSIITMSVLDD598 pKa = 3.76NDD600 pKa = 4.12AFNGGSIDD608 pKa = 3.95LNEE611 pKa = 4.16VTLTPGVPSDD621 pKa = 4.58PNLSMNADD629 pKa = 3.44GTINIPASTRR639 pKa = 11.84PGTYY643 pKa = 8.8TYY645 pKa = 9.94PYY647 pKa = 8.92TICEE651 pKa = 4.03VLNPSNCSTAVATIVIEE668 pKa = 4.07ASNLFIPNVITPNGDD683 pKa = 3.27GQNDD687 pKa = 3.35RR688 pKa = 11.84FEE690 pKa = 6.18IIGSEE695 pKa = 3.82NYY697 pKa = 10.29DD698 pKa = 3.28RR699 pKa = 11.84VEE701 pKa = 3.88VTIVNRR707 pKa = 11.84WGNEE711 pKa = 2.88IFRR714 pKa = 11.84NDD716 pKa = 3.42NYY718 pKa = 11.29DD719 pKa = 3.68NNWTGRR725 pKa = 11.84GLNEE729 pKa = 3.37GTYY732 pKa = 10.27YY733 pKa = 10.89YY734 pKa = 10.06IIKK737 pKa = 9.95LYY739 pKa = 10.35KK740 pKa = 8.96GSKK743 pKa = 9.16VEE745 pKa = 3.97QHH747 pKa = 6.41KK748 pKa = 10.69GWVLIKK754 pKa = 9.01THH756 pKa = 5.97

Molecular weight: 79.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U0P0R3|A0A4U0P0R3_9SPHI GtrA family protein OS=Sphingobacterium olei OX=2571155 GN=FAZ15_12560 PE=3 SV=1
MM1 pKa = 7.5SGSIHH6 pKa = 6.15PTRR9 pKa = 11.84DD10 pKa = 3.03DD11 pKa = 5.03AYY13 pKa = 10.44FKK15 pKa = 11.08PNDD18 pKa = 3.53IPHH21 pKa = 6.68LKK23 pKa = 10.38EE24 pKa = 4.58SICHH28 pKa = 5.5NPAGISPEE36 pKa = 4.02RR37 pKa = 11.84NEE39 pKa = 3.72VRR41 pKa = 11.84PNTGSVLLNRR51 pKa = 11.84SDD53 pKa = 3.89APPIPRR59 pKa = 11.84SIHH62 pKa = 5.38FLRR65 pKa = 11.84NDD67 pKa = 3.13VPLKK71 pKa = 10.26RR72 pKa = 11.84NEE74 pKa = 3.96VRR76 pKa = 11.84PKK78 pKa = 10.5ARR80 pKa = 11.84NIFLNTPSVRR90 pKa = 11.84HH91 pKa = 5.55MPQSIYY97 pKa = 9.7FVRR100 pKa = 11.84VNVHH104 pKa = 6.12HH105 pKa = 6.6KK106 pKa = 10.93RR107 pKa = 11.84NDD109 pKa = 3.16GSLNAGRR116 pKa = 11.84VFLNPPSVCHH126 pKa = 6.43IPQSIYY132 pKa = 9.36FVRR135 pKa = 11.84VNVYY139 pKa = 10.2HH140 pKa = 7.24KK141 pKa = 10.99

Molecular weight: 16.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4341

0

4341

1597812

20

3026

368.1

41.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.965 ± 0.032

0.695 ± 0.01

5.638 ± 0.022

6.052 ± 0.032

4.838 ± 0.025

6.859 ± 0.035

1.944 ± 0.018

7.306 ± 0.033

6.425 ± 0.039

9.398 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.013

5.673 ± 0.033

3.656 ± 0.021

3.764 ± 0.023

4.275 ± 0.026

6.562 ± 0.024

5.722 ± 0.032

6.401 ± 0.027

1.26 ± 0.015

4.371 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski