Pasteurella multocida (strain Pm70)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Pasteurella; Pasteurella multocida; Pasteurella multocida subsp. multocida

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2007 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9CK60|Q9CK60_PASMU Uncharacterized protein OS=Pasteurella multocida (strain Pm70) OX=272843 GN=PM1774 PE=4 SV=1
MM1 pKa = 7.95LDD3 pKa = 3.58TNPPAEE9 pKa = 4.32SQYY12 pKa = 11.07KK13 pKa = 7.81PTYY16 pKa = 10.92ADD18 pKa = 4.19ILAQLNDD25 pKa = 3.41IQEE28 pKa = 4.37SLEE31 pKa = 3.76IAIDD35 pKa = 3.31KK36 pKa = 10.14GYY38 pKa = 8.61PTIKK42 pKa = 10.71AEE44 pKa = 4.09GVPDD48 pKa = 3.27IRR50 pKa = 11.84MSDD53 pKa = 3.19KK54 pKa = 11.11DD55 pKa = 3.69YY56 pKa = 11.68LEE58 pKa = 4.3WALEE62 pKa = 4.22RR63 pKa = 11.84INCLIYY69 pKa = 10.62DD70 pKa = 3.82VEE72 pKa = 4.61NIQEE76 pKa = 4.32KK77 pKa = 10.07LAQLLQINCDD87 pKa = 3.97TNCEE91 pKa = 4.02LL92 pKa = 5.06

Molecular weight: 10.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7DAY1|Q7DAY1_PASMU HexD OS=Pasteurella multocida (strain Pm70) OX=272843 GN=hexD PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84SRR14 pKa = 11.84THH16 pKa = 6.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2007

0

2007

666911

37

3919

332.3

37.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.582 ± 0.065

1.088 ± 0.022

4.907 ± 0.045

6.102 ± 0.055

4.426 ± 0.043

6.55 ± 0.06

2.37 ± 0.028

6.855 ± 0.056

5.922 ± 0.05

10.922 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.028

4.323 ± 0.046

3.859 ± 0.029

5.099 ± 0.064

4.445 ± 0.043

5.663 ± 0.035

5.298 ± 0.039

6.767 ± 0.049

1.171 ± 0.022

3.226 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski