Defluviitoga tunisiensis

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Petrotogales; Petrotogaceae; Defluviitoga

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1811 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C7P419|A0A0C7P419_DEFTU SGNH hydrolase subfamily protein OS=Defluviitoga tunisiensis OX=1006576 GN=DTL3_1325 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.13RR3 pKa = 11.84ILGILSVLLVLAVSVLAVADD23 pKa = 4.05TEE25 pKa = 4.56TVPVYY30 pKa = 10.94LEE32 pKa = 3.91VPAFVRR38 pKa = 11.84ITVNEE43 pKa = 4.13EE44 pKa = 3.21VDD46 pKa = 3.61GAFNLVFDD54 pKa = 4.78PADD57 pKa = 3.78PEE59 pKa = 4.58STVEE63 pKa = 3.99DD64 pKa = 4.19TVALLAEE71 pKa = 4.59ANVNYY76 pKa = 10.6DD77 pKa = 2.51VDD79 pKa = 4.18YY80 pKa = 11.5SLLPVQSYY88 pKa = 10.51SNWANLISVTIDD100 pKa = 2.98KK101 pKa = 10.63SVSGFGQPGEE111 pKa = 4.06VSFNATAYY119 pKa = 10.28FNATAYY125 pKa = 10.04INDD128 pKa = 3.69YY129 pKa = 10.58LSYY132 pKa = 9.81GTVPANTHH140 pKa = 5.35IADD143 pKa = 3.73VVFTISSII151 pKa = 3.56

Molecular weight: 16.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C7P131|A0A0C7P131_DEFTU Ribosomal RNA small subunit methyltransferase G OS=Defluviitoga tunisiensis OX=1006576 GN=rsmG PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.17QPSKK9 pKa = 8.92IKK11 pKa = 10.49RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 6.75GFLARR21 pKa = 11.84KK22 pKa = 6.71STPGGRR28 pKa = 11.84NVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.42GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1811

0

1811

601742

37

1648

332.3

37.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.22 ± 0.051

0.7 ± 0.016

5.386 ± 0.041

7.723 ± 0.059

5.138 ± 0.049

6.124 ± 0.05

1.398 ± 0.021

9.748 ± 0.052

8.602 ± 0.056

9.614 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.215 ± 0.025

5.88 ± 0.052

3.419 ± 0.028

2.656 ± 0.028

3.445 ± 0.037

6.276 ± 0.04

4.757 ± 0.033

6.405 ± 0.042

0.892 ± 0.023

4.402 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski