Diplodia corticola

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetes incertae sedis; Botryosphaeriales; Botryosphaeriaceae; Diplodia

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10836 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J9RLD5|A0A1J9RLD5_9PEZI Ste ste20 ysk protein kinase OS=Diplodia corticola OX=236234 GN=BKCO1_3100084 PE=4 SV=1
MM1 pKa = 7.4SPHH4 pKa = 6.95LSFLSATLAVFPFWVSPVSTIALSSLKK31 pKa = 10.77NEE33 pKa = 4.18STPAAAHH40 pKa = 6.57LDD42 pKa = 3.35IATSSGTTVSSTPPAQLAASITPTPANSTTQHH74 pKa = 6.68DD75 pKa = 4.27ASDD78 pKa = 3.57AAALSRR84 pKa = 11.84NRR86 pKa = 11.84ACEE89 pKa = 3.9SLIQSWNSASASFAASILAGSPDD112 pKa = 3.49AAASALATIPHH123 pKa = 6.01SLITTTYY130 pKa = 8.28PGGYY134 pKa = 8.45TISTSYY140 pKa = 11.35NPLLLPHH147 pKa = 6.25FTSTPSAPSTPSPNATISPQPSASVSTPCADD178 pKa = 5.0RR179 pKa = 11.84IPRR182 pKa = 11.84APGAAYY188 pKa = 10.69SLLTVATASPTTLVSTRR205 pKa = 11.84IAYY208 pKa = 7.69EE209 pKa = 3.84ALVPAPACAYY219 pKa = 10.02DD220 pKa = 3.67RR221 pKa = 11.84RR222 pKa = 11.84GCALEE227 pKa = 4.51LAEE230 pKa = 5.25KK231 pKa = 10.41PKK233 pKa = 9.95TLPLCAQGGNVAAGFVNDD251 pKa = 4.5EE252 pKa = 4.35KK253 pKa = 11.22CVLPSGVVGCRR264 pKa = 11.84VAAGGVGVYY273 pKa = 7.83YY274 pKa = 10.12WPRR277 pKa = 11.84EE278 pKa = 4.04DD279 pKa = 4.34GGEE282 pKa = 4.24GACAGGGEE290 pKa = 4.55GEE292 pKa = 4.57GEE294 pKa = 4.41GEE296 pKa = 4.29GKK298 pKa = 10.62VGVSAAPTTTKK309 pKa = 7.45TTKK312 pKa = 9.77TVVVGTNTFTSPTVYY327 pKa = 10.66VSFSEE332 pKa = 3.98IDD334 pKa = 3.19AAPYY338 pKa = 9.83CLPTYY343 pKa = 10.25RR344 pKa = 11.84NVVVGLPPEE353 pKa = 4.38SVSWVSMSRR362 pKa = 11.84GWPITKK368 pKa = 10.08SLNWNDD374 pKa = 3.35LNYY377 pKa = 9.68PVPSTAYY384 pKa = 9.92HH385 pKa = 6.24GMEE388 pKa = 3.88ACQVNTAALRR398 pKa = 11.84PDD400 pKa = 3.74GCATIYY406 pKa = 11.13DD407 pKa = 4.38DD408 pKa = 6.35DD409 pKa = 4.13YY410 pKa = 11.63HH411 pKa = 7.23PYY413 pKa = 10.37LALPTDD419 pKa = 3.65PALYY423 pKa = 9.69QQALGGSDD431 pKa = 3.18GGGGFANCTALTWQKK446 pKa = 11.02YY447 pKa = 6.55VTDD450 pKa = 4.31PPIPLTTAGALIPQSSEE467 pKa = 3.63VPAATTTTTTTTGADD482 pKa = 3.74PEE484 pKa = 4.72STTAAAPVTEE494 pKa = 4.77TNAPTAPATATATTGGGGGNNAQATGSSSAGSSDD528 pKa = 3.54PADD531 pKa = 4.0PSATSAVVDD540 pKa = 3.73TNPWDD545 pKa = 3.72NLGSMISEE553 pKa = 4.36AWSTSFADD561 pKa = 4.13PAAEE565 pKa = 4.07TATTTTGTAAGQGGGGEE582 pKa = 4.36ASDD585 pKa = 3.8GLPVVEE591 pKa = 5.22TSAPASSAPEE601 pKa = 4.25PPSSAAVVATIGTAVASPAAPASAADD627 pKa = 3.87SSDD630 pKa = 3.24PTATATAGNNNDD642 pKa = 4.17NNDD645 pKa = 4.74DD646 pKa = 4.0DD647 pKa = 6.1DD648 pKa = 4.83EE649 pKa = 5.14NNNSNSNNSDD659 pKa = 3.39NNDD662 pKa = 3.33NGSPGGSQTLPAGHH676 pKa = 6.18TTATAPLYY684 pKa = 10.2TPTASSSPAPASPAPPIAITLSDD707 pKa = 3.98DD708 pKa = 3.62SGSGSGSGSGSHH720 pKa = 7.07VIAADD725 pKa = 3.72PSDD728 pKa = 3.63PTAGVIVVGGGAGGDD743 pKa = 3.73AQEE746 pKa = 4.91DD747 pKa = 4.16ASTQTIAAGATTTIDD762 pKa = 3.2GTQVVVSSVPAAEE775 pKa = 4.67GDD777 pKa = 3.74SDD779 pKa = 5.85GDD781 pKa = 5.23DD782 pKa = 4.96SDD784 pKa = 6.6DD785 pKa = 6.38DD786 pKa = 6.47DD787 pKa = 7.7DD788 pKa = 7.64DD789 pKa = 7.65DD790 pKa = 7.68DD791 pKa = 7.68DD792 pKa = 7.63DD793 pKa = 7.58DD794 pKa = 7.46DD795 pKa = 7.79DD796 pKa = 5.46DD797 pKa = 4.57AQPPAVVVVVVGGSRR812 pKa = 11.84TYY814 pKa = 9.91TLPGGATTGGSGSGSGSGGGIVGATTVPLSSLAAGTAGGAGRR856 pKa = 11.84TGAVVTFTAGGAGGGGEE873 pKa = 4.27KK874 pKa = 9.69TLSAAQLAPGTVVVGGKK891 pKa = 7.04TLSAGGPAATVTVSGTGSGLGSGLSGDD918 pKa = 3.97GEE920 pKa = 4.85VVVVASVAPGGGLVVGSAGGGGGGSSSTSRR950 pKa = 11.84RR951 pKa = 11.84LSSAAATASRR961 pKa = 11.84TMSADD966 pKa = 2.87NTATRR971 pKa = 11.84NSALPTTTATATTPDD986 pKa = 5.02DD987 pKa = 5.03DD988 pKa = 7.05DD989 pKa = 6.33SDD991 pKa = 5.38NDD993 pKa = 5.03DD994 pKa = 5.75DD995 pKa = 6.99DD996 pKa = 6.86DD997 pKa = 6.02DD998 pKa = 4.6SQSTDD1003 pKa = 2.91AAAAAATATTAGAASGGSVPPLLAMLCSFLLTCSIPSVFSIFAA1046 pKa = 4.02

Molecular weight: 101.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J9R6V3|A0A1J9R6V3_9PEZI Branchpoint-bridging protein OS=Diplodia corticola OX=236234 GN=BKCO1_12000130 PE=3 SV=1
MM1 pKa = 7.34ATKK4 pKa = 10.4QSTKK8 pKa = 9.15YY9 pKa = 10.05EE10 pKa = 4.05RR11 pKa = 11.84LEE13 pKa = 4.08GLSQRR18 pKa = 11.84RR19 pKa = 11.84GVDD22 pKa = 3.3TQHH25 pKa = 6.39AQQAEE30 pKa = 3.94RR31 pKa = 11.84DD32 pKa = 3.68RR33 pKa = 11.84RR34 pKa = 11.84LRR36 pKa = 11.84WAQEE40 pKa = 3.87MIMQNPALCAEE51 pKa = 5.15VYY53 pKa = 10.47CAMSKK58 pKa = 10.28RR59 pKa = 11.84PAMPPRR65 pKa = 11.84RR66 pKa = 11.84EE67 pKa = 4.01SSLSSSSSSSRR78 pKa = 11.84RR79 pKa = 11.84SPSTAAGSMPSPGISSNSSINSGSPTT105 pKa = 3.32

Molecular weight: 11.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10836

0

10836

5743668

55

7609

530.1

58.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.894 ± 0.026

1.162 ± 0.009

5.859 ± 0.02

6.145 ± 0.024

3.52 ± 0.015

7.395 ± 0.028

2.432 ± 0.011

4.136 ± 0.016

4.472 ± 0.018

8.523 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.067 ± 0.008

3.39 ± 0.013

6.43 ± 0.033

3.901 ± 0.019

6.345 ± 0.023

8.097 ± 0.03

5.946 ± 0.024

6.227 ± 0.018

1.464 ± 0.009

2.592 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski