Rhodococcus phage Takoda

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rerduovirus; Rhodococcus virus Takoda

Average proteome isoelectric point is 5.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4Q8F9|A0A2Z4Q8F9_9CAUD Tape measure protein OS=Rhodococcus phage Takoda OX=2201455 GN=21 PE=4 SV=1
MM1 pKa = 7.64IEE3 pKa = 4.26SKK5 pKa = 10.99SLGEE9 pKa = 3.8VSGFEE14 pKa = 4.6IEE16 pKa = 5.05AGWIYY21 pKa = 11.35DD22 pKa = 3.59DD23 pKa = 5.01VGPDD27 pKa = 3.51FYY29 pKa = 11.57GDD31 pKa = 3.54EE32 pKa = 4.51VYY34 pKa = 11.07DD35 pKa = 4.0DD36 pKa = 3.87QTIEE40 pKa = 4.25AYY42 pKa = 9.81EE43 pKa = 3.88NNEE46 pKa = 3.39WHH48 pKa = 6.38FVVLIVEE55 pKa = 4.27ARR57 pKa = 11.84FNGHH61 pKa = 5.81VMGTAYY67 pKa = 10.87LGGVEE72 pKa = 4.51VGFFPGVAEE81 pKa = 4.48PLDD84 pKa = 4.03PLTNSDD90 pKa = 4.19YY91 pKa = 11.2LDD93 pKa = 4.26DD94 pKa = 4.15MVAEE98 pKa = 4.69AVNSARR104 pKa = 11.84IEE106 pKa = 4.07LQDD109 pKa = 4.02TITKK113 pKa = 10.0GLEE116 pKa = 3.75VLLL119 pKa = 4.65

Molecular weight: 13.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q8A2|A0A2Z4Q8A2_9CAUD Cas4 family exonuclease OS=Rhodococcus phage Takoda OX=2201455 GN=56 PE=4 SV=1
MM1 pKa = 7.85RR2 pKa = 11.84SMGKK6 pKa = 10.02AIKK9 pKa = 8.42WTFKK13 pKa = 10.46ATMFLALATVSVIVVLGGLLVLVAGVRR40 pKa = 11.84KK41 pKa = 9.82LL42 pKa = 3.4

Molecular weight: 4.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14075

42

1115

210.1

23.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.641 ± 0.479

0.817 ± 0.116

6.65 ± 0.206

7.417 ± 0.431

3.453 ± 0.186

8.54 ± 0.397

2.075 ± 0.189

4.647 ± 0.219

3.901 ± 0.22

8.476 ± 0.341

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.38 ± 0.183

3.545 ± 0.201

4.703 ± 0.22

3.218 ± 0.14

5.968 ± 0.323

5.812 ± 0.217

6.011 ± 0.248

7.801 ± 0.252

1.84 ± 0.169

3.105 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski